Biological Characteristics of Fusarium oxysporum f. sp.niveum Isolated from Watermelon Cultivated in Plastic Greenhouse and Indoor Toxicity Determination of Different Fungicides in Hainan Province of China

[ Objective] The paper was to study biological characteristics of Fusarium oxysporum f. sp. niveum in watermelon cultivated in plastic greenhouse in Hainan Province of China and to determine indoor toxicity of fungicides. [ Method] Effects of various factors on mycelial growth and spore germination were stud- ied, and the lethal temperature of spores and indoor toxicity of fungicides were also determined. [Result] The fungal mycelium had a better growth rate on water- melon juice medium than the others. The optimum temperature for mycelial growth and spore germination were 28 ℃ and 30 ℃, and the optimum pH values were 7 - 8 and 7 - 9, respectively. Light-dark alternation was conducive for mycelial growth. The lethal temperature of spore was 60 ℃ for 5 min. The fungal mycelium grew better while taking fructose and glucose as carbon sources and yeast extract and peptone as nitrogen sources. Toxicity determination showed that 50% pro- chloraz-manganese chloride complex WP had the greatest inhibition effect on mycelial growth, with the ECso value of 0.730 9 μg/mL; followed by 32.5% benzoic azoxystrobin SC, 30% benzoylate· propieanazol EC, 10% difenoconazole WG, 25% bromothalonil · carbendazim WP and 25% bromothalonil WP, with the ECso values from 1.884 7μg/mL to 8. 161 0μg/mL. [Conclusion] The study provided basic data for field control against F. oxysporum f. sp. niveum on watermelon cultivated in plastic greenhouse in Hainan Province.

Combined effects of enhanced ultraviolet-B radiation and doubled CO_(2) concentration on growth,fruit quality and yield of tomato in winter plastic greenhouse

Five different doses of ultraviolet-B(UV-B)radiation were supplied to tomato(Lycopersicon esculeutum.Mill)with the doubled CO_(2)concentration(700μmol·mol^(-1))in the winter plastic greenhouse.The influences on the seedling growth,fruit quality and yield of tomato were investigated.Results showed that the seedling growth,and the contents of UV absorbing compounds,soluble sugar,organic acid,vitamin C and lycopene of tomato fruits,and yield of tomato increased under doubled CO_(2)concentration.Under the doubled CO_(2)concentration the effects of lost doses of UV-B radiation could further promote the effects of doubled CO_(2)concentration.However,there is no significant increase in yield of tomato.The best dose of UV-B radiation is about 1.163 kJ·m^(-2).When the dose of UV-B radiation is more than it,the effects of UV-B will be reduced.

Analysis of Phthalate Esters in Air, Soil and Plants in Plastic Film Greenhouse

The phthalate esters such as DMP, DEP, DBP and DEHP in air, soil and plant samples in plastic film greenhouse were clean up with fine silica gel column and determined with HPLC. It was found that the concentrations of PEs in air and soil samples in plastic film greenhouse are much higher than those of contrast samples. But concentrations of PEs in plants in plastic film greenhouse are not remarkably affected by the pollution of air and soil.

Effects of plastic film mulching on soil greenhouse gases(CO2,CH4 and N2O) concentration within soil profiles in maize fields on the Loess Plateau,China

To better understand the effects of plastic film mulching on soil greenhouse gases（GHGs） emissions,we compared seasonal and vertical variations of GHG concentrations at seven soil depths in maize（Zea mays L.） fields at Changwu station in Shaanxi,a semi-humid region,between 2012 and 2013.Gas samples were taken simultaneously every one week from non-mulched（BP） and plastic film-mulched（FM） field plots.The results showed that the concentration of GHGs varied distinctly at the soil-atmosphere interface and in the soil profile during the maize growing season（MS）.Both carbon dioxide（CO_2） and nitrous oxide（N_2O） concentrations increased with increasement of soil depth,while the methane（CH_4）concentrations decreased with increasement of soil depth.A strong seasonal variation pattern was found for CO_2 and N_2O concentrations,as compared to an inconspicuous seasonal variation of CH_4 concentrations.The mean CO_2 and N_2O concentrations were higher,but the mean CH_4 concentration in the soil profiles was lower in the FM plots than in the BP plots.The results of this study suggested that plastic film mulching significantly increased the potential emissions of CO_2and N_2O from the soil,and promoted CH_4 absorption by the soil,particularly during the MS.

CFD Based Study of Heterogeneous Microclimate in a Typical Chinese Greenhouse in Central China

Indoor microclimate is important for crop production and quality in greenhouse cultivation. This paper focuses on microclimate study based on a computational fluid dynamics （CFD） model of a typical plastic greenhouse （with a sector shape vertical cross-section） popularly used in central China. A radiation model is added into the CFD model so as to simulate coupling of convective transfers and radiative exchanges at the cover and the roof, instead of using the usual coupling approach based on energy balance. In addition, a fractal permeability model is innovatively adopted in the modeling of the crop canopy. Compared the numerical results with measured experimental data, the model simulation is proved with success. This model then is used to explore the microclimate variable distributions in the greenhouse. It shows that the airflow pattern, temperature and humidity profiles are different from those in a sawtooth Mediterranean- type greenhouse. The study suggests that this deliberately developed CFD model can be served as a useful tool in macroclimate research and greenhouse design investigating.

Irrigation system and land use effect on surface water quality in river, at lake Dianchi, Yunnan, China

The surface water samples were collected in river Dahe and its tributaries, which flow into severely eutrophic lake Dianchi, Yunnan Province, China, in order to elucidate factors controlling water quality fluctuations. The temporal and spatial distribution of water quality tendency was observed. The water quality of each river is dependent on the hydrology effect such water gate and circulating irrigation system. We must consider the hydrology effect to accurately understand water quality variations of river in this study field. In river without highly circulating irrigation system or water gate effect, the downstream nitrate nitrogen （NO3-N） concentration increase occurred in area dominated by open field cultivation, whereas the NO3-N concentration was constant or decreased in area dominated by greenhouse land use. This result suggests that greenhouse covers the soil from precipitation, and nitrate load of greenhouse could be less than that of open field cultivation while the rainfall event. In the upper reaches of river, where is dominated by open field cultivation, there were no sharp increase dissolved molybdate reactive phosphorus and total phosphorus concentration, but P load was accumulated in the lower reaches of river, whose predominant land use is greenhouse. Although the P sources is unclear in this study, greenhouse area may have potential of P loads due to its high P content in greenhouse soil. Considering hydrology effect is necessary to determine what the major factor is influencing the water quality variation, especially in area with highly complicated irrigation system in this studying site.