Thrombosis formation on disrupted atherosclerotic plaque is the most common acuse of cardiovascular diseases, in the pathophysiology, increased platelet reactivity is a descriptor of the risk of cardiovascular events ...Thrombosis formation on disrupted atherosclerotic plaque is the most common acuse of cardiovascular diseases, in the pathophysiology, increased platelet reactivity is a descriptor of the risk of cardiovascular events in healthy persons and in patients with overt coronary artery disease. Regardless of the stimulus for activation platelet-platelet interation and thrombus formation is ultimately regulated through the GP Ⅱ b/Ⅲ a receptor展开更多
Fetal liver tissues obtained from 28 human fetuses with gestation age from 3 to 6 months and fetal bone marrow from 35 human fetuses from 3 to 7 months were observed by immunochemical staining with anti-platelet GPⅡ ...Fetal liver tissues obtained from 28 human fetuses with gestation age from 3 to 6 months and fetal bone marrow from 35 human fetuses from 3 to 7 months were observed by immunochemical staining with anti-platelet GPⅡ b / Ⅲa monoclonal antibody and ABC technique. In the fetal liver, megakaryocytes were wholly located among growing fetal liver cells and near foci of hemopoiesis. Some megakaryocytes in the fetal liver were small7890- lymphoid-like megakaryocytes. The size of megakaryocytes both in the fetal liver (14.79 ± 4.52μm) and in the fetal bone marrow (16.08±7.39 μm) was small, which did not vary significantly over the gestation age ranging from 3 to 6 or 7 months. However, the maturation stage of megakaryocytes in the fetal liver shifted to more mature stage with the advancement of gestation, although the maturation stage of megakaryocytes in the fetal bone marrow did not change with the advancement of gestation from 4 to 7 months, the megakaryocyte in the fetal bone marrow was less mature展开更多
目的探讨冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的变化。方法采用流式细胞仪三色免疫荧光分析检测健康老年人和冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的阳性百分率、蛋白酪氨酸磷酸酶(CD148)的几何荧光强度、P-选择素(CD6...目的探讨冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的变化。方法采用流式细胞仪三色免疫荧光分析检测健康老年人和冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的阳性百分率、蛋白酪氨酸磷酸酶(CD148)的几何荧光强度、P-选择素(CD62P)的阳性百分率、P-选择素(CD62P)的几何荧光强度及膜糖蛋白Ⅱb/Ⅲa复合物(PAC-1)的阳性百分率、膜糖蛋白Ⅱb/Ⅲa复合物(PAC-1)的几何荧光强度的表达水平。结果冠心病患者和健康老年人的血小板膜CD62P、CD62P几何荧光强度表达量相比较(6.24 vs 0.89,P<0.01;45.64 vs 25.38,P<0.05),差异有统计学意义;CD148、CD148几何荧光强度表达量相比较(7.12 vs 0.45,P<0.01;41.35 vs 20.12,P<0.01),差异有统计学意义;PAC-1及PAC-1几何荧光强度的表达量(22.64 vs 1.84,P<0.01;43.46 vs 22.45,P<0.01),差异有统计学意义,但与当日血压、血糖及血脂无相关关系。结论 CD62P及PAC-1、CD148是血小板活化的敏感指标,冠心病患者有血小板活化现象,为冠心病患者提供了理论检测指标。展开更多
Platelet activation plays an important role in thrombosis. Platelet glycoprotein Ⅱ b/Ⅲ a ( GPⅡ b/Ⅲ a ) is the receptor of fibrinogen. Platelet cross-linking with fibrinogen through GP Ⅱ b/Ⅲ a is the process of...Platelet activation plays an important role in thrombosis. Platelet glycoprotein Ⅱ b/Ⅲ a ( GPⅡ b/Ⅲ a ) is the receptor of fibrinogen. Platelet cross-linking with fibrinogen through GP Ⅱ b/Ⅲ a is the process of thrombosis. Ca^2+ is an important intracellular second messenger in platelet activation. It has been reported that GP Ⅱ b/Ⅲ a receptors were involved in the calcium influx of activated platelet, and GP Ⅱ b/Ⅲ a receptor had characteristics of calcium channel or an adjacent calcium channel.展开更多
Batifiban, a synthetic cyclic peptide, is a potent platelet glycoprotein GPⅡb/Ⅲa antagonist which may be useful in the treatment and prevention of acute coronary syndromes. The pharmacokinetics and pharmacodymanic ...Batifiban, a synthetic cyclic peptide, is a potent platelet glycoprotein GPⅡb/Ⅲa antagonist which may be useful in the treatment and prevention of acute coronary syndromes. The pharmacokinetics and pharmacodymanic (inhibition of platelet aggregation) effects, and tolerability of batifiban were investigated in healthy subjects following single bolus injection with doses of 55, 110, or 220 μg/kg, or multiple doses of an bolus followed intravenous infusion for 24 h (180 μg/kg plus 2.0 μg/minokg, and 220 μg/kg plus 2.5μg/minokg) in this phase I clinical trial. Plasma levels of batifiban and areas under the curve were found to be proportional to doses. Batifiban was rapidly eliminated with a half-life of approximately 2.5 h. Significant differences were noted for plasma levels of batifiban and areas under the curve between males and females. No significant differences in the terminal half-life were found between males and females. Batifiban reversibly inhibited ex vivo platelet aggregation in a dose- and concentration-dependent manner, consistent with its mechanism as a GPⅡ b/Ⅲa antagonist. Single and multiple intravenous doses of batifiban were found to be safe and well tolerated in healthy subjects. These results support a bolus injection plus intravenous infusion regimen of batifiban for the treatment and prevention of acute coronary syndromes.展开更多
To compare clinical outcomes and safety of eptifibatide or tirofiban in patients with acute coronary syndrome(ACS) undergoing percutaneous coronary intervention(PCI). Methods:Thirty-six patients with ACS(unstabl...To compare clinical outcomes and safety of eptifibatide or tirofiban in patients with acute coronary syndrome(ACS) undergoing percutaneous coronary intervention(PCI). Methods:Thirty-six patients with ACS(unstable angina/non-ST-segment elevation myocardial infarction, UA/NSTEMI) who underwent PCI were randomly divided into two groups to receive eptifibatide or tirofiban treatment. Eptifibatide or tirofiban was predominantly initiated in the catheter laboratory before the intervention. In-hospital and 30-day MACE outcomes; bleeding as well as platelet counting were investigated in those two groups. Results:No in-hospital and 30-day MACE event occurred in the two groups. The number of ischemia leads after treatment reduced compared to that before PCI in the two groups. There was improvement in the number of ischemia leads for 24 h after administration in the tirofiban group than those in eptifibatide group(4.21 ± 2.46 vs. 3.89 ± 3.31, P =0.03). The two groups showed no incidence of massive bleeding. Minor bleeding rates were 16.7% and 22.2% in the two groups respectively. Conclusion:Eptifibatide as an adjunct to PCI may further decrease the incidence of ischemia event in patients with ACS and improve the safety, but its long-term efficacy and side effects need further observation.展开更多
Objective:To evaluate the efficacy and safety of tirofiban hydrochloride in the treatment of ischemic stroke with thrombolytic therapy.Method:Two hundred patients with acute ischemic stroke thrombolysis were randomly ...Objective:To evaluate the efficacy and safety of tirofiban hydrochloride in the treatment of ischemic stroke with thrombolytic therapy.Method:Two hundred patients with acute ischemic stroke thrombolysis were randomly divided into the experimental group and the control group.The experimental group was given tirofiban with the addition of rtpa in the control group and the therapeutic effects of the two groups were compared.Results:In comparison with the control group,the NIHSS improvement rate was 98%in the experimental group within 14 days.The platelet aggregation rate and efficacy in the experimental group were significantly reduced than the control group(P<0.01).The major adverse reaction in the two groups was hemorrhage with an incidence rate of 3%.Conclusion:Tirofiban hydrochloride is a highly effective and selective platelet glycoprotein Ⅱb/Ⅲa receptor inhibitor,which is safe and effective in combination with heparin and aspirin.展开更多
This paper designed a detailed procedure for monogenic hypertension diagnosis by Whole Exome Sequencing(WES)and provided reliable precise medication guidance.Identification of mutated points provides the clinician val...This paper designed a detailed procedure for monogenic hypertension diagnosis by Whole Exome Sequencing(WES)and provided reliable precise medication guidance.Identification of mutated points provides the clinician valuable information for effective individualized therapeutic option.Therapeutic options that specifically restore the pathway disturbed by these point mutations can be selected to give a precise medicinal guidance.展开更多
目的观察不同血管分型的进展性缺血性脑卒中(PIS)的基线特征及应用替罗非班的临床效果。方法回顾性选取2020年3月—2022年3月湘潭市中心医院收治的PIS患者89例,根据治疗方法不同分为观察组42例和对照组47例。对照组患者予以常规治疗及...目的观察不同血管分型的进展性缺血性脑卒中(PIS)的基线特征及应用替罗非班的临床效果。方法回顾性选取2020年3月—2022年3月湘潭市中心医院收治的PIS患者89例,根据治疗方法不同分为观察组42例和对照组47例。对照组患者予以常规治疗及阿司匹林100 mg联合氢氯吡格雷75 mg抗血小板聚集,观察组患者在对照组基础上加用替罗非班治疗,根据责任血管(小动脉闭塞型和大动脉粥样硬化型)进一步亚组分析,比较各组NIHSS评分、住院时间,随访90 d,比较各组mRS评分、焦虑、抑郁及胃肠道出血发生率。结果观察组与对照组及各亚组基线特征、入院时NIHSS评分、加重时NIHSS评分比较差异均无统计学意义(P>0.05);观察组治疗7 d后NIHSS评分低于对照组,ΔNIHSS评分及随访90 d mRS评分良好率高于对照组,住院时间短于对照组(P<0.05)。根据责任血管分型的亚组分析显示,在小动脉闭塞亚组中,观察亚组加重7 d后的NIHSS评分低于对照亚组,ΔNIHSS评分及随访90 d mRS评分良好率高于对照亚组,住院时间短于对照亚组(P<0.05);而在大动脉粥样硬化亚组中,2亚组患者基线特征、入院时NIHSS评分、加重时NIHSS评分、治疗7 d后NIHSS评分、ΔNIHSS评分、住院时间及随访90 d mRS评分良好率比较差异均无统计学意义(P>0.05)。随访90 d,观察组与对照组及各亚组焦虑、抑郁、胃肠道出血发生率比较差异均无统计学意义(P>0.05)。结论与口服抗血小板药物比较,替罗非班对不同责任血管导致的PIS疗效可能存在差异,替罗非班可显著改善小动脉闭塞型PIS的神经功能缺损,疗效确切,且安全性高,值得推广应用。展开更多
文摘Thrombosis formation on disrupted atherosclerotic plaque is the most common acuse of cardiovascular diseases, in the pathophysiology, increased platelet reactivity is a descriptor of the risk of cardiovascular events in healthy persons and in patients with overt coronary artery disease. Regardless of the stimulus for activation platelet-platelet interation and thrombus formation is ultimately regulated through the GP Ⅱ b/Ⅲ a receptor
文摘Fetal liver tissues obtained from 28 human fetuses with gestation age from 3 to 6 months and fetal bone marrow from 35 human fetuses from 3 to 7 months were observed by immunochemical staining with anti-platelet GPⅡ b / Ⅲa monoclonal antibody and ABC technique. In the fetal liver, megakaryocytes were wholly located among growing fetal liver cells and near foci of hemopoiesis. Some megakaryocytes in the fetal liver were small7890- lymphoid-like megakaryocytes. The size of megakaryocytes both in the fetal liver (14.79 ± 4.52μm) and in the fetal bone marrow (16.08±7.39 μm) was small, which did not vary significantly over the gestation age ranging from 3 to 6 or 7 months. However, the maturation stage of megakaryocytes in the fetal liver shifted to more mature stage with the advancement of gestation, although the maturation stage of megakaryocytes in the fetal bone marrow did not change with the advancement of gestation from 4 to 7 months, the megakaryocyte in the fetal bone marrow was less mature
文摘目的探讨冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的变化。方法采用流式细胞仪三色免疫荧光分析检测健康老年人和冠心病患者血小板膜上蛋白酪氨酸磷酸酶(CD148)的阳性百分率、蛋白酪氨酸磷酸酶(CD148)的几何荧光强度、P-选择素(CD62P)的阳性百分率、P-选择素(CD62P)的几何荧光强度及膜糖蛋白Ⅱb/Ⅲa复合物(PAC-1)的阳性百分率、膜糖蛋白Ⅱb/Ⅲa复合物(PAC-1)的几何荧光强度的表达水平。结果冠心病患者和健康老年人的血小板膜CD62P、CD62P几何荧光强度表达量相比较(6.24 vs 0.89,P<0.01;45.64 vs 25.38,P<0.05),差异有统计学意义;CD148、CD148几何荧光强度表达量相比较(7.12 vs 0.45,P<0.01;41.35 vs 20.12,P<0.01),差异有统计学意义;PAC-1及PAC-1几何荧光强度的表达量(22.64 vs 1.84,P<0.01;43.46 vs 22.45,P<0.01),差异有统计学意义,但与当日血压、血糖及血脂无相关关系。结论 CD62P及PAC-1、CD148是血小板活化的敏感指标,冠心病患者有血小板活化现象,为冠心病患者提供了理论检测指标。
文摘Platelet activation plays an important role in thrombosis. Platelet glycoprotein Ⅱ b/Ⅲ a ( GPⅡ b/Ⅲ a ) is the receptor of fibrinogen. Platelet cross-linking with fibrinogen through GP Ⅱ b/Ⅲ a is the process of thrombosis. Ca^2+ is an important intracellular second messenger in platelet activation. It has been reported that GP Ⅱ b/Ⅲ a receptors were involved in the calcium influx of activated platelet, and GP Ⅱ b/Ⅲ a receptor had characteristics of calcium channel or an adjacent calcium channel.
文摘Batifiban, a synthetic cyclic peptide, is a potent platelet glycoprotein GPⅡb/Ⅲa antagonist which may be useful in the treatment and prevention of acute coronary syndromes. The pharmacokinetics and pharmacodymanic (inhibition of platelet aggregation) effects, and tolerability of batifiban were investigated in healthy subjects following single bolus injection with doses of 55, 110, or 220 μg/kg, or multiple doses of an bolus followed intravenous infusion for 24 h (180 μg/kg plus 2.0 μg/minokg, and 220 μg/kg plus 2.5μg/minokg) in this phase I clinical trial. Plasma levels of batifiban and areas under the curve were found to be proportional to doses. Batifiban was rapidly eliminated with a half-life of approximately 2.5 h. Significant differences were noted for plasma levels of batifiban and areas under the curve between males and females. No significant differences in the terminal half-life were found between males and females. Batifiban reversibly inhibited ex vivo platelet aggregation in a dose- and concentration-dependent manner, consistent with its mechanism as a GPⅡ b/Ⅲa antagonist. Single and multiple intravenous doses of batifiban were found to be safe and well tolerated in healthy subjects. These results support a bolus injection plus intravenous infusion regimen of batifiban for the treatment and prevention of acute coronary syndromes.
基金supported by a grant from ShenZhen Hybio Engineering Co.,Ltd.
文摘To compare clinical outcomes and safety of eptifibatide or tirofiban in patients with acute coronary syndrome(ACS) undergoing percutaneous coronary intervention(PCI). Methods:Thirty-six patients with ACS(unstable angina/non-ST-segment elevation myocardial infarction, UA/NSTEMI) who underwent PCI were randomly divided into two groups to receive eptifibatide or tirofiban treatment. Eptifibatide or tirofiban was predominantly initiated in the catheter laboratory before the intervention. In-hospital and 30-day MACE outcomes; bleeding as well as platelet counting were investigated in those two groups. Results:No in-hospital and 30-day MACE event occurred in the two groups. The number of ischemia leads after treatment reduced compared to that before PCI in the two groups. There was improvement in the number of ischemia leads for 24 h after administration in the tirofiban group than those in eptifibatide group(4.21 ± 2.46 vs. 3.89 ± 3.31, P =0.03). The two groups showed no incidence of massive bleeding. Minor bleeding rates were 16.7% and 22.2% in the two groups respectively. Conclusion:Eptifibatide as an adjunct to PCI may further decrease the incidence of ischemia event in patients with ACS and improve the safety, but its long-term efficacy and side effects need further observation.
文摘Objective:To evaluate the efficacy and safety of tirofiban hydrochloride in the treatment of ischemic stroke with thrombolytic therapy.Method:Two hundred patients with acute ischemic stroke thrombolysis were randomly divided into the experimental group and the control group.The experimental group was given tirofiban with the addition of rtpa in the control group and the therapeutic effects of the two groups were compared.Results:In comparison with the control group,the NIHSS improvement rate was 98%in the experimental group within 14 days.The platelet aggregation rate and efficacy in the experimental group were significantly reduced than the control group(P<0.01).The major adverse reaction in the two groups was hemorrhage with an incidence rate of 3%.Conclusion:Tirofiban hydrochloride is a highly effective and selective platelet glycoprotein Ⅱb/Ⅲa receptor inhibitor,which is safe and effective in combination with heparin and aspirin.
文摘This paper designed a detailed procedure for monogenic hypertension diagnosis by Whole Exome Sequencing(WES)and provided reliable precise medication guidance.Identification of mutated points provides the clinician valuable information for effective individualized therapeutic option.Therapeutic options that specifically restore the pathway disturbed by these point mutations can be selected to give a precise medicinal guidance.
文摘目的观察不同血管分型的进展性缺血性脑卒中(PIS)的基线特征及应用替罗非班的临床效果。方法回顾性选取2020年3月—2022年3月湘潭市中心医院收治的PIS患者89例,根据治疗方法不同分为观察组42例和对照组47例。对照组患者予以常规治疗及阿司匹林100 mg联合氢氯吡格雷75 mg抗血小板聚集,观察组患者在对照组基础上加用替罗非班治疗,根据责任血管(小动脉闭塞型和大动脉粥样硬化型)进一步亚组分析,比较各组NIHSS评分、住院时间,随访90 d,比较各组mRS评分、焦虑、抑郁及胃肠道出血发生率。结果观察组与对照组及各亚组基线特征、入院时NIHSS评分、加重时NIHSS评分比较差异均无统计学意义(P>0.05);观察组治疗7 d后NIHSS评分低于对照组,ΔNIHSS评分及随访90 d mRS评分良好率高于对照组,住院时间短于对照组(P<0.05)。根据责任血管分型的亚组分析显示,在小动脉闭塞亚组中,观察亚组加重7 d后的NIHSS评分低于对照亚组,ΔNIHSS评分及随访90 d mRS评分良好率高于对照亚组,住院时间短于对照亚组(P<0.05);而在大动脉粥样硬化亚组中,2亚组患者基线特征、入院时NIHSS评分、加重时NIHSS评分、治疗7 d后NIHSS评分、ΔNIHSS评分、住院时间及随访90 d mRS评分良好率比较差异均无统计学意义(P>0.05)。随访90 d,观察组与对照组及各亚组焦虑、抑郁、胃肠道出血发生率比较差异均无统计学意义(P>0.05)。结论与口服抗血小板药物比较,替罗非班对不同责任血管导致的PIS疗效可能存在差异,替罗非班可显著改善小动脉闭塞型PIS的神经功能缺损,疗效确切,且安全性高,值得推广应用。