[Objectives]To explore the optimal extraction process and the antioxidant activity of flavonoidsof Cacumen platycladi. [Methods]FCPwas obtained from C. platycladi by a new set extraction process of microwave-assisted ...[Objectives]To explore the optimal extraction process and the antioxidant activity of flavonoidsof Cacumen platycladi. [Methods]FCPwas obtained from C. platycladi by a new set extraction process of microwave-assisted extraction,freeze thawing,superfine crushingand freeze drying technology and purification. On the basis of single-factor tests,the quadratic regression parasitism model was established by the extraction amount of total flavonoids with microwave power,alcohol concentration,and liquid-to-solid ratio through the response surface methodology. [Results] The optimal extraction conditions were microwave power of 757 W,alcohol concentration of 69%,liquid-to-solid ratio of21∶ 1,with the optimal extraction yield of 15. 302 9 mg/g. Under the real conditions( microwave power of 800 W,alcohol concentration of69%,liquid-to-solid ratio of 21∶ 1) in our laboratory,the maximum yield of total flavonoids was 15. 9 mg/g,the results fitwell with the prediction of quadratic regression parasitism model. The good antioxidant activity has been determined byscavenging effect of FCP on 1,1-diphenyl-2-picrylhydrazyl( DPPH) radical and superoxide anion free radical with the scavenging yield of 70. 2% and 68. 8% respectively. [Conclusions]All results established a good foundation for furtherdevelopment of C. platycladi products.展开更多
红色毛癣菌(Trichophyton rubrum,T.rubrum)是皮肤癣最主要病原;侧柏叶(Cacumen Platycladi)是侧柏[Platycladus orientali s(L.)Franco]的干燥嫩枝梢及叶,被《苗族医学》收录用于治疗皮肤癣。本研究旨在探讨侧柏叶抗T.rubrum的主要活...红色毛癣菌(Trichophyton rubrum,T.rubrum)是皮肤癣最主要病原;侧柏叶(Cacumen Platycladi)是侧柏[Platycladus orientali s(L.)Franco]的干燥嫩枝梢及叶,被《苗族医学》收录用于治疗皮肤癣。本研究旨在探讨侧柏叶抗T.rubrum的主要活性物质及其抗真菌机制,为抗皮肤癣新药研发提供帮助。本研究通过测定最小抑菌浓度和孢子萌发率分析了侧柏叶活性物质对T.rubrum的抑菌活性,使用电镜观察T.rubrum菌丝形态和孢子超微结构,并检测侧柏叶活性物质对T.rubrum细胞膜通透性、完整性和麦角甾醇含量的影响,从而阐述侧柏叶活性物质对T.rubrum抑菌机理,最后采用GC-MS和qRT-PCR方法找到其抗T.rubrum的作用靶点。结果显示,侧柏叶石油醚部位的α-蒎烯为主要抗菌活性成分,MIC为32μg·mL^(-1),极显著抑制孢子萌发(P<0.01);α-蒎烯可致细胞膜发生明显损伤和内容物流出、通透性极显著增加(P<0.01)以及24 h内细胞膜中麦角甾醇和24(28)脱氢麦角甾醇含量显著下降(P<0.05);引起脂质色谱图中ERG3活性抑制相关的“麦角甾烷-7,24-二烯-3β-醇”色谱峰出现;可极显著降低ERG3的相对表达量(P<0.01)。结果表明,α-蒎烯是侧柏叶中发挥抗T.rubrum活性最主要成分且以ERG3为其抗真菌靶点。展开更多
基金Sponsored by Qing Lan Project of Jiangsu Province(2016)Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals(JSBGFC12010)+1 种基金Excellent Scientific and Technological Innovation Team of Universities in Jiangsu Province(2017-31)Nanjing Polytechnic Institute NHKY-2017-09
文摘[Objectives]To explore the optimal extraction process and the antioxidant activity of flavonoidsof Cacumen platycladi. [Methods]FCPwas obtained from C. platycladi by a new set extraction process of microwave-assisted extraction,freeze thawing,superfine crushingand freeze drying technology and purification. On the basis of single-factor tests,the quadratic regression parasitism model was established by the extraction amount of total flavonoids with microwave power,alcohol concentration,and liquid-to-solid ratio through the response surface methodology. [Results] The optimal extraction conditions were microwave power of 757 W,alcohol concentration of 69%,liquid-to-solid ratio of21∶ 1,with the optimal extraction yield of 15. 302 9 mg/g. Under the real conditions( microwave power of 800 W,alcohol concentration of69%,liquid-to-solid ratio of 21∶ 1) in our laboratory,the maximum yield of total flavonoids was 15. 9 mg/g,the results fitwell with the prediction of quadratic regression parasitism model. The good antioxidant activity has been determined byscavenging effect of FCP on 1,1-diphenyl-2-picrylhydrazyl( DPPH) radical and superoxide anion free radical with the scavenging yield of 70. 2% and 68. 8% respectively. [Conclusions]All results established a good foundation for furtherdevelopment of C. platycladi products.
