Removal of competitive native species combined with tree planting can accelerate the initial afforestation process: an experiment in an old field in Japan invaded by dwarf bamboo and kudzu

Restoring natural forests after field abandon- ment is a land management objective that fosters the re- covery of forest biodiversity. We performed seeding and transplanting of native tree species 40 years after the abandonment of an arable field that became dominated by a dwarf bamboo （Pleioblastus chino （Franch. et Sav.） Makino） and by kudzu （Pueraria lobata （Willd.） Ohwi）. By permutation tests, the removal of competing vegetation （gap creation） significantly increased the survival of three seeded species of Fagaceae and of eight transplanted spe- cies. In contrast, intact vegetation prevented most indi- viduals of all species from surviving for 1 year after planting. The lack of natural recruitment of Fagaceae in the nonseeded subplots indicated that seed limitation was a cause of the slow afforestation. Although litter accumula- tion in gaps at the time of seeding slightly increased sur- vival for late-germinating Quercus myrsinifolia Blume andCastanopsis sieboldii （Makino） Hatus. ex T. Yamaz. et Mashiba, the effect was not consistent among plots and was not statistically significant. Our results suggest that for successful afforestation using native trees in abandoned fields, it will be necessary to remove competitive native species to avoid severe limitations on microsite availability and that simultaneous tree establishment by seeding or transplanting should be implemented to accelerate the establishment of native tree species.

Interactions among dwarf bamboo, litter and consumption by small vertebrates place multiple constraints on the establishment of native tree seedlings in a Japanese agricultural landscape

Aims Faunal assemblage alterations due to anthropogenic impacts have changed herbivore-vegetation relationships in various ecosystems,but the influences of small vertebrates on revegetation processes remain unclear.In a peri-urban agricultural landscape in eastern Japan that lacks large ungulates but supports small generalist herbivores,fewer native seedlings have become established in thickets dominated by native dwarf bamboo,Pleioblastus chino(Franch.et Sav.)Makino.The mechanisms limiting tree seedling establishment are unknown.Our aim here was to evaluate the influence of interactions among the dwarf bamboo,its litter cover and small vertebrate herbivores on the micro-site conditions governing the establishment of native tree seedlings from different successional stages in an old field in temperate Japan.Methods First,seedling emergence and survival of seeded Pinus densiflora Siebold et Zucc.,Celtis sinensis Pers.,Aphananthe aspera(Thunb.)Planch.,Quercus serrata Murray,Quercus myrsinifolia Blume and Castanopsis sieboldii(Makino)Hatus.ex T.Yamaz.et Mashiba were observed in a factorial design that accounted for the presence or absence of dwarf bamboo and its litter cover,as well as the exclu-sion or access of small vertebrate fauna,in 2009 and 2010.To identify small vertebrate herbivores that reduced the emergence or survival of tree seedlings in the first experiment,reseeding experi-ments using P.densiflora,C.sinensis,A.aspera and Q.myrsinifolia were conducted by video trapping in 2011 and video and still-cam-era trapping in 2012.Important Findings The abiotic conditions under the dwarf bamboo were lethal to early-to mid-successional P.densiflora,C.sinensis,A.aspera and Q.serrata but led to better survival-although spindly growth-of late-succes-sional Q.myrsinifolia and C.sieboldii.The main consumers and the plants they found palatable differed between the bamboo thickets and the intervening gaps.Predation by mice appeared to have severely limited the emergence of all species,particularly in the bamboo thick-ets but occasionally in the gaps,whereas litter cover slightly mitigated this limitation.However,bamboo litter cover reduced the emergence of P.densiflora in the gaps.Chinese bamboo partridge(Bambusicola thoracicus Temminck),an introduced game bird,selectively and severely fed on the cotyledons-and probably seeds-of A.aspera and moderately preyed upon those of P.densiflora in the gaps.Apparent consumption of seedlings in the gaps by the Japanese hare(Lepus brachyurus Temminck)suppressed the growth of all species but apparently did not severely affect emergence and survival.Thus,the net balance of positive and negative interactions at the bamboo thicket scale(i.e.the presence of dominant dwarf bamboo cover or intervening gaps and the herbivore behaviours in these two environments)and at the microsite level(i.e.litter cover)shapes the early establishment patterns of native tree seedlings,and this balance differs among tree seedling species.