In order to investigate the intratumoral DNA ploid heterogeneity (PH) in esophageal squamous cell carcinoma and its clinical-pathological significance, nuclear DNA ploidy of 80 cases of squamous cell carcinoma of the ...In order to investigate the intratumoral DNA ploid heterogeneity (PH) in esophageal squamous cell carcinoma and its clinical-pathological significance, nuclear DNA ploidy of 80 cases of squamous cell carcinoma of the esophagus were determined with multiple samples removed from the same tumor, using a flow cytometry (FCM) technique. 240 samples for flow cytimetric DNA analysis were taken from 3 different parts if each tumor of 80 cases of specimens. DNA measurement was were present in a tumor or the variation in DI value among 3 peaks in each tumor was greater than 10%. Further more at last, comparison or clinical-pathological characteristics was performed between PHTs and N-PHT which has a similar ploid pattern in 3 sampling spots of each tumor (non PHT). DNA indices ranged from 0.77～1.74, and the incidence of DNA AN was 88. 8% (71/80) in this series. Of 80 cases, 38 cases (47. 5%, 38/80) showed intratumoral heterogeneity in DNA ploidy. The heterogeneity in DNA ploidy was related to the extent of wall penetration by the tumor,the incidence of lymph node metastasis and the patients's prognosis, not to histological grades and size of the tumor. There is PH phenomena in esophageal squmous cell carcinoma, and DNA PH may be a more exact indicator in reflecting the biological chatacteristics of the tumor and patient's prognosis.展开更多
Fifty-one cases of nephroblastoma were pathologically studied by light and electron microscopy as well as microspectrophotometry. Under light and electron microscope, the tumor appeared to be carcinosarcoma structural...Fifty-one cases of nephroblastoma were pathologically studied by light and electron microscopy as well as microspectrophotometry. Under light and electron microscope, the tumor appeared to be carcinosarcoma structurally, consisting of epithelial, sarcomatous and undlfferentiated tumor cells. Their ultrastructural feature was similar to that of renal tubular epithelial cells and tibroblast. It could differentially be diagnosed from renal metoblastic tumors by electron microscopy when the tumor was mainly composed of sarcomatous cells. Microspectrophotometer measurement showed an Increased mean nuclear DNA content (DI>1. 04), belonging to aneuplold tumor, which together with polyploid cells, serving as one of the Important characteristics of malignant tumors. In addition, the poorer the tumor differentiation the higher was the DNA content of the tumor cells, suggesting that the measurement of nuclear DNA of tumor cells might also serve as one of signs to acertain the degree of differentiation and malignant of nephroblastomas with more preciseneas than general morphological examinations.展开更多
Objective: To study the value of DNA ploid detection for the evaluation of cancer cell proliferation and invasion activity in cervical cancer. Methods: Patients with cervical cancer who underwent surgical resection in...Objective: To study the value of DNA ploid detection for the evaluation of cancer cell proliferation and invasion activity in cervical cancer. Methods: Patients with cervical cancer who underwent surgical resection in Yan'an University Affiliated Hospital between March 2015 and May 2017 were selected as cervical cancer group of the research, and patients with benign lesions who underwent hysterectomy in Yan'an University Affiliated Hospital during the same period were selected as the control group of the research. Before operation, cervical exfoliated cytology was taken for DNA ploid detection;before operation, the cervical tissue was taken to determine the expression of proliferation genes and invasion genes. Results: The number of exfoliated cells with abnormal DNA ploid in cervical cancer group was significantly higher than that in control group;Id-1, Ki-67, TET1, S6K1, CatL and ILK mRNA expression in cervical cancer tissue of cervical cancer group were significantly higher than those of control group whereas HSG, MCPH1, p16, TIMP1 and TIMP2 mRNA expression were significantly lower than those of control group;Id-1, Ki-67, TET1, S6K1, CatL and ILK mRNA expression in cervical cancer tissue with 3 exfoliated cells with abnormal DNA ploid were significantly higher than those in cervical cancer tissue with 1-2 exfoliated cells with abnormal DNA ploid whereas HSG, MCPH1, p16, TIMP1 and TIMP2 mRNA expression were significantly lower than those in cervical cancer tissue with 1-2 exfoliated cells with abnormal DNA ploid. Conclusion: DNA ploid detection can be used to evaluate the proliferation and invasion of cancer cells in cervical cancer.展开更多
文摘In order to investigate the intratumoral DNA ploid heterogeneity (PH) in esophageal squamous cell carcinoma and its clinical-pathological significance, nuclear DNA ploidy of 80 cases of squamous cell carcinoma of the esophagus were determined with multiple samples removed from the same tumor, using a flow cytometry (FCM) technique. 240 samples for flow cytimetric DNA analysis were taken from 3 different parts if each tumor of 80 cases of specimens. DNA measurement was were present in a tumor or the variation in DI value among 3 peaks in each tumor was greater than 10%. Further more at last, comparison or clinical-pathological characteristics was performed between PHTs and N-PHT which has a similar ploid pattern in 3 sampling spots of each tumor (non PHT). DNA indices ranged from 0.77～1.74, and the incidence of DNA AN was 88. 8% (71/80) in this series. Of 80 cases, 38 cases (47. 5%, 38/80) showed intratumoral heterogeneity in DNA ploidy. The heterogeneity in DNA ploidy was related to the extent of wall penetration by the tumor,the incidence of lymph node metastasis and the patients's prognosis, not to histological grades and size of the tumor. There is PH phenomena in esophageal squmous cell carcinoma, and DNA PH may be a more exact indicator in reflecting the biological chatacteristics of the tumor and patient's prognosis.
文摘Fifty-one cases of nephroblastoma were pathologically studied by light and electron microscopy as well as microspectrophotometry. Under light and electron microscope, the tumor appeared to be carcinosarcoma structurally, consisting of epithelial, sarcomatous and undlfferentiated tumor cells. Their ultrastructural feature was similar to that of renal tubular epithelial cells and tibroblast. It could differentially be diagnosed from renal metoblastic tumors by electron microscopy when the tumor was mainly composed of sarcomatous cells. Microspectrophotometer measurement showed an Increased mean nuclear DNA content (DI>1. 04), belonging to aneuplold tumor, which together with polyploid cells, serving as one of the Important characteristics of malignant tumors. In addition, the poorer the tumor differentiation the higher was the DNA content of the tumor cells, suggesting that the measurement of nuclear DNA of tumor cells might also serve as one of signs to acertain the degree of differentiation and malignant of nephroblastomas with more preciseneas than general morphological examinations.
文摘Objective: To study the value of DNA ploid detection for the evaluation of cancer cell proliferation and invasion activity in cervical cancer. Methods: Patients with cervical cancer who underwent surgical resection in Yan'an University Affiliated Hospital between March 2015 and May 2017 were selected as cervical cancer group of the research, and patients with benign lesions who underwent hysterectomy in Yan'an University Affiliated Hospital during the same period were selected as the control group of the research. Before operation, cervical exfoliated cytology was taken for DNA ploid detection;before operation, the cervical tissue was taken to determine the expression of proliferation genes and invasion genes. Results: The number of exfoliated cells with abnormal DNA ploid in cervical cancer group was significantly higher than that in control group;Id-1, Ki-67, TET1, S6K1, CatL and ILK mRNA expression in cervical cancer tissue of cervical cancer group were significantly higher than those of control group whereas HSG, MCPH1, p16, TIMP1 and TIMP2 mRNA expression were significantly lower than those of control group;Id-1, Ki-67, TET1, S6K1, CatL and ILK mRNA expression in cervical cancer tissue with 3 exfoliated cells with abnormal DNA ploid were significantly higher than those in cervical cancer tissue with 1-2 exfoliated cells with abnormal DNA ploid whereas HSG, MCPH1, p16, TIMP1 and TIMP2 mRNA expression were significantly lower than those in cervical cancer tissue with 1-2 exfoliated cells with abnormal DNA ploid. Conclusion: DNA ploid detection can be used to evaluate the proliferation and invasion of cancer cells in cervical cancer.
