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Effects of Shenggu Injection (生骨注射液) on mRNA Expression of Vascular Endothelia Growth Factor in Rat Osteoblasts in vitro 被引量:2
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作者 程杰 彭锐 +3 位作者 余渊 王永清 邹阳 夏仁云 《Chinese Journal of Integrated Traditional and Western Medicine》 2005年第3期191-194,共4页
Objective: To study the effects of Shenggu injection (生骨注射液,SGI) on mRNA expression of vascular endothelia growth factor (VEGF) in rat osteoblasts in vitro and to explore its possible molecular mechanisms in... Objective: To study the effects of Shenggu injection (生骨注射液,SGI) on mRNA expression of vascular endothelia growth factor (VEGF) in rat osteoblasts in vitro and to explore its possible molecular mechanisms in promoting fracture healing. Methods: Rat osteoblasts cultured in vitro were stimulated with SGI according to the protocol. The expression levels of VEGF mRNA in the cells in every group were examined by reverse-transcriptase ploymerase chain reaction (RT-POR). Results: When osteoblasts were stimulated with different concentrations of SGI for 5 days, the expression of VEGF mRNA peaked with 1 mg/ml SGI on the 5th day. When treated with 1 mg/ml SGI from the 1st to the 5th day, the expression of VEGF mRNA increased gradually with the increase of culturing time. Conclusion: SGI could promote significantly the expression of VEGF mRNA in rat osteoblasts in vitro. The levels of expression of VEGF mRNA changed along with different concentrations and stimulating time of SGI. 展开更多
关键词 Shenggu injection osteoblast vascular endothelia growth factor reverse-transcriptase ploymerase chain reaction fracture healing
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