RNA interference(RNAi)technology is a promising crop protection strategy against agricultural pests.The soybean pod borer(SPB),Leguminivora glycinivorella(Mats.),is a serious pest of soybean in northeastern Asia.The n...RNA interference(RNAi)technology is a promising crop protection strategy against agricultural pests.The soybean pod borer(SPB),Leguminivora glycinivorella(Mats.),is a serious pest of soybean in northeastern Asia.The neonate larvae of SPB are endophagous,initially feeding on the inner layer of the pod and then on the immature soybean seeds,a behavior that makes them difficult to rear.Therefore,a suitable artificial diet is desirable to facilitate RNAi research.Seven artificial diets and soybean pods were used for rearing SPB neonate larvae.The survival and mean weight of the larvae reared on artificial diet 6(A6,included 2 g soybean flour and 10 g R3 immature soybean pod powder)were found to be significantly higher than those for larvae reared on all other diets.Subsequently,A6 diet was prepared in DEPC-treated water to obtain a RNase-free artificial diet(RF).The survival and mean weight of the larvae were not significantly different between A6 and RF diets.Feeding neonate larvae with RF-SpbP0 dsRNA(ribosomal protein P0)silenced SpbP0 gene,resulting in arrested development and increased mortality.The results of this study suggested that the newly developed A6 or RF diets could rear SPB neonate larvae and help towards developing an effective method for oral delivery of dsRNA to SPB larvae.展开更多
Information on mechanisms and inheritance of resistance is critical to plan an effective strategy to breed for resistance to insect pests. Therefore, we evaluated a diverse array of chickpea genotypes (eight desi and ...Information on mechanisms and inheritance of resistance is critical to plan an effective strategy to breed for resistance to insect pests. Therefore, we evaluated a diverse array of chickpea genotypes (eight desi and one kabuli) with varying levels of resistance to the pod borer, Helicoverpa armigera to gain an understanding of the nature of gene action and possible maternal effects. The test genotypes were crossed in all possible combinations for a full diallel. The 72 F1s (36 direct and 36 reciprocal crosses) along with the parents were evaluated for resistance to H. armigera under field conditions, and for antibiosis mechanism of resistance (larval survival and larval weight gain) by using detached leaf assay under laboratory conditions, and grain yield under un-protected conditions in the field. Additive gene action governed the inheritance of resistance to H. armigera, while non-additive type of gene action was predominant for inheritance of antibiosis component of resistance (larval survival and larval weight) and grain yield. Greater magnitude of σ2 A(17.39 and 1.42) than σ2 D (3.93 and 1.21) indicated the preponderance of σ2 Ain inheritance of resistance to pod borer, H. armigera under laboratory and field conditions, respectively. There were no maternal effects for inheritance of resistance to pod borer and grain yield. Lines with significant gca effects for pod borer damage and grain yield were identified for further use in the resistance breeding program. The implications of the inheritance pattern of pod borer resistance and grain yield are discussed in the context of strategies to enhance pod borer resistance and grain yield in chickpea.展开更多
基金Supported by the Harbin Science and Technology Innovation Program(2017RAQXJ122)China National Novel Transgenic Organisms Breeding Project(2016ZX08004-004-006)+2 种基金the Heilongjiang Natural Science Foundation(C2018012)the Chinese National Natural Science Foundation(31201229)Soybean Research&Development Center(CARS-04-PS04)。
文摘RNA interference(RNAi)technology is a promising crop protection strategy against agricultural pests.The soybean pod borer(SPB),Leguminivora glycinivorella(Mats.),is a serious pest of soybean in northeastern Asia.The neonate larvae of SPB are endophagous,initially feeding on the inner layer of the pod and then on the immature soybean seeds,a behavior that makes them difficult to rear.Therefore,a suitable artificial diet is desirable to facilitate RNAi research.Seven artificial diets and soybean pods were used for rearing SPB neonate larvae.The survival and mean weight of the larvae reared on artificial diet 6(A6,included 2 g soybean flour and 10 g R3 immature soybean pod powder)were found to be significantly higher than those for larvae reared on all other diets.Subsequently,A6 diet was prepared in DEPC-treated water to obtain a RNase-free artificial diet(RF).The survival and mean weight of the larvae were not significantly different between A6 and RF diets.Feeding neonate larvae with RF-SpbP0 dsRNA(ribosomal protein P0)silenced SpbP0 gene,resulting in arrested development and increased mortality.The results of this study suggested that the newly developed A6 or RF diets could rear SPB neonate larvae and help towards developing an effective method for oral delivery of dsRNA to SPB larvae.
文摘Information on mechanisms and inheritance of resistance is critical to plan an effective strategy to breed for resistance to insect pests. Therefore, we evaluated a diverse array of chickpea genotypes (eight desi and one kabuli) with varying levels of resistance to the pod borer, Helicoverpa armigera to gain an understanding of the nature of gene action and possible maternal effects. The test genotypes were crossed in all possible combinations for a full diallel. The 72 F1s (36 direct and 36 reciprocal crosses) along with the parents were evaluated for resistance to H. armigera under field conditions, and for antibiosis mechanism of resistance (larval survival and larval weight gain) by using detached leaf assay under laboratory conditions, and grain yield under un-protected conditions in the field. Additive gene action governed the inheritance of resistance to H. armigera, while non-additive type of gene action was predominant for inheritance of antibiosis component of resistance (larval survival and larval weight) and grain yield. Greater magnitude of σ2 A(17.39 and 1.42) than σ2 D (3.93 and 1.21) indicated the preponderance of σ2 Ain inheritance of resistance to pod borer, H. armigera under laboratory and field conditions, respectively. There were no maternal effects for inheritance of resistance to pod borer and grain yield. Lines with significant gca effects for pod borer damage and grain yield were identified for further use in the resistance breeding program. The implications of the inheritance pattern of pod borer resistance and grain yield are discussed in the context of strategies to enhance pod borer resistance and grain yield in chickpea.
