Electroacupuncture at the Renzhong (DU 26) and Neiguan (PC 6) acupoints repairs the DNA of nervous tissue supplied by the middle cerebral artery in rats

This study compares the changes of DNA repair in brain tissue dominated by the middle cerebral artery after electroacupuncture at the acupoints of Renzhong （DU 26）, Neiguan （PC 6）, Quchi （LI 11） and Zusanli （ST 36） in rats. In the apurinic/apyrimidinic endonuclease DNA basic group reparative excision experiments, the apurinic/apyrimidinic endonuclease activity of brain tissue increased slightly after electroacupuncture in rats. In the DNA polymerase β （Pol β）experiments, the Pol β ac-tivity of brain tissue increased after electroacupuncture, especially at DU 26 and PC 6. In the DNA ligase experiments, the expression of DNA ligase 1 in brain tissue increased significantly after electroacupuncture. These findings demonstrate increased activity of apurinic/apyrimidinic en-donuclease, Pol β and DNA ligase 1 after electroacupuncture at DU 26 and PC 6. Also, DNA repair in brain tissue supplied by the middle cerebral artery is promoted after electroacupuncture at DU 26 and PC 6, which are more effective than the LI 11 and ST 36 acupoints .

CLINICAL APPLICATION OF NEIGUAN (内关 PC 6) IN THE TREATMENT OF DISORDERS OF THE DIGESTIVE SYSTEM——THE RELATED RESEARCH PROGRESS

In the present paper, the authors review recent situations of clinical application of Neiguan (PC 6内关), etc. in the treatment of disorders of the digestive system and its related research progresses. In clinical practice, Neiguan (PC 6) is usually selected as one of the main acupoints to treat gastrointestinal reactions induced by ① nausea and vomiting, ② hiccup, ③ gastrointestinal dyskinesia, ④ gastritis, and ⑤ intra-gastric examination, cesarean section, etc. Concerning experimental studies, the effects of Neiguan (PC 6), etc. on ① gastric electrical activities, ② gastrointestinal motion, ③ gastrointestinal hormone secretion, and ④ gastric mucosal structure were observed. Results indicated that Neiguan (PC 6) is an important and effective acupoint for treatment of gastrointestinal disorders in clinic. Stimulation of Neiguan (PC 6) induced favorable regulation of both the peripheral nervous system and central nervous system, and changes of the gastrointestinal hormone secretion may contribute to its effects in treating various disorders.

Effect of electroacupuncture at Neiguan(PC6)at different time points on myocardial ischemia reperfusion arrhythmia in rats

OBJECTIVE:To observe the effects of electroacupuncture at Neiguan(PC6)at different time points on reperfusion arrhythmia(RA)after myocardial ischemia and reperfusion in rats,and to investigate the correlation of this protective effect with nerve growth factor(NGF),tyrosine kinase A(Trk A),tyrosine hydroxylase(TH),and norepinephrine(NE).METHODS:A total of 72 Sprague-Dawley male rats were randomly divided into six groups(n=12 rats/group):normal group(Norm),sham operation group(Sham),ischemia reperfusion group(I/R),pre-ischemic electroacupuncture group(EAI),pre-reperfusion electroacupuncture group(EAII),post-reperfusion electroacupuncture group(EAIII).The myocardial ischemia-reperfusion injury(MIRI)model was induced by occlusion of left anterior descending coronary artery for 20 min followed by reperfusion for 40 min in rats.With no intervention in the Norm group and only threading without ligation in the Sham group.Electroacupuncture pretreatment at 20 min/d for 7 d before ligation in the EAⅠgroup,20 min of electroacupuncture before reperfusion in the EAII group and 20 min of electroacupuncture after reperfusion in the EAIII group.The electrocardiogram(ECG)of each group was recorded throughout the whole process,and the success of the MIRI model was determined based on the changs of J-point and T-wave in the ECG.The arrhythmia score was used to record premature ventricular contractions,ventricular tachycardia and ventricular fibrillation during the reperfusion period to assess the reperfusion induced arrhythmias.The expression levels of NGF,Trk A,TH protein were measured by Western blot.Moreover,the expression levels of plasma and myocardial NE levels were detected by enzyme linked immunosorbent assay.RESULTS:The differences between Norm group and Sham group were not statistically significant in all indexes.Arrhythmia score,myocardial NGF,Trk A,TH,and NE expression were significantly higher in the I/R group compared with the Sham group.Arrhythmia score,myocardial NGF,Trk A,TH,and NE expression were significantly lower in each EA group compared with the I/R group.CONCLUSION:Electroacupuncture at Neiguan(PC6)at different time points can reduce the incidence and severity of reperfusion arrhythmias in rats.This protective effect is related to electroacupuncture regulating NGF,Trk A,TH,NE expression and reducing sympathetic hyperactivation.

NEIGUAN(PC 6) BLOCKING FOR PREVENTING NAUSEA AND VOMITING CAUSED BY SUBARACHOID MORPHINE ANALGESIA

To find a simple and an inexpensive by method to reduce postoperative nausea and vom iting abbreviated to (PONV) caused subarachoid morphine analgesia. Methods: ninty-eight gyneeco logic patients who took 0. 5 mg morphine by subarahaid space to control postoperative pain, randomly divided into group Ⅰ (n = 50) whose bilateral Neiguan were blocked by 0. 25% bupivacaine 2 ml, group Ⅱ (n= 48), who were intravenously injected ondansetron 4 mg, preoperatively and postopera tively to prevent nausea and vomiting. The numbers of the patients experienced PONV were observed for 48 hrs after operation. Result: group i had 15 patients, group Ⅱ had 14 ones, who experienced ~ (P >0. 05 ), Conclusion: Neiguan blocking can effectively prevnt PONV caused by subara choid morphine anslgesia without side effects.

内关、太冲穴位埋线对自发性高血压前期大鼠心肌损伤和能量代谢的影响

目的:探讨内关、太冲穴位埋线对自发性高血压(SHR)前期大鼠心肌损伤和能量代谢的影响。方法:选择16只4周龄自发性高血压大鼠,随机分为埋线组与模型组(n=8),另取8只同龄的京都种Wistar(WKY)大鼠为对照组。埋线组大鼠定位内关、太冲并进行穴位埋线干预,模型组及对照组仅予以抓取固定干预,实验周期为2周。在干预前1 h及干预2、7、14 d后,测定各组大鼠收缩压(SBP)、舒张压(DBP)、平均动脉压(MAP)及心率(HR);并取心肌组织,采用HE染色比较各组大鼠左心室前壁心肌组织病理改变,电镜观察左心室前壁心肌组织线粒体的结构差异,酶联免疫吸附试验(ELISA)试剂盒测定心肌组织血管紧张素Ⅱ(AngⅡ)水平,三磷酸腺苷(ATP)检测试剂盒测定心肌组织中ATP水平。结果:干预2、7和14 d后,与模型组比较,埋线组大鼠DBP和MBP显著降低(P<0.01);干预7 d和14 d后,与模型组比较,埋线组大鼠SBP明显降低(P<0.01)。3组大鼠HR比较,差异无统计学意义(P>0.05)。模型组心肌细胞排列紊乱,心肌细胞横径和细胞间隙明显增大;与模型组比较,埋线组心肌细胞排列规则,心肌细胞横径明显减小(P<0.05),细胞间隙基本正常。在高倍透射电镜下,模型组线粒体排列紊乱,形状不规则,线粒体密度减小,埋线组较模型组有所改善。与模型组比较,埋线组左心室心肌组织中ATP水平升高,AngⅡ水平降低(P<0.05)。结论:内关、太冲穴位埋线可调节自发性高血压前期大鼠心肌能量代谢,对改善心肌损伤和降低血压水平有积极作用。

针刺内关穴治疗低脉压综合症106例临床观察

平均动脉压正常而脉压差降低，可以产生一系列临床表现，可谓之低脉压综合症。针刺双侧内关穴具有升高收缩压、降低舒张压、增大脉压差并消除相应的临床症状的作用，有效率在９２％以上。

Effect of electronic stimulation at Neiguan(PC 6) acupoint on gene expression of adenosine triphosphate-sensitive potassium channel and protein kinases in rats with myocardial ischemia

OBJECTIVE:To investigate the effects of electronic stimulation at acupoints Neiguan(PC 6) and Lieque(LU 7) on the gene expression of the adenosine triphosphate(ATP)-Sensitive potassium channel(KATP:Kir6.1,Kir6.2,SUR2 A,and SUR2B) and protein kinases(PKA,PKG,and PKCβ2) in myocardial cells of rats with myocardial ischemia(Ml) induced by isoproterenol(ISO).METHODS:Rats were randomly divided into a control,model,Neiguan(PC 6),Lieque(LU 7),and non-acupoint groups.The Ml model was established by injecting rats with ISO.Electro-acupuncture treatment was given to the acupuncture groups,once a day for 7 days.Gene expression was analyzed with real-time PCR.RESULTS:The gene expression of KATP and protein kinases in the model group was higher than those in the control group(P < 0.05).After acupuncture treatment,the KATP and protein kinase expression levels were significantly lower in the Neiguan(PC 6)and Lieque(LU 7) groups compared with the model group[P < 0.05).The Neiguan(PC 6) group lowered these levels significantly more than that of the Lieque(LU 7) group(P < 0.05).No significant differences were observed between the model and non-acupoint groups(P > 0.05).CONCLUSION:Our findings suggest that electronic needling of Neiguan(PC 6) can both reduce the gene expression of KATP and protein kinases in rats with ISO-induced Ml.

Effect of Acupuncture at Acupoint Neiguan(PC6)on the Myocardial c-fos Gene Expression of Acute Myocardial Ischemia in Rats

Objective：To investigate the mechanism of improvement effect of electro acupuncture at acupoint Neiguan （PC 6） on acute myocardial ischemia （AMI）. Methods： Ninety-six rats were randomly divided into sham-operation group, myocardial ischemia model group and myocardial ischemia model plus electro-acupuncture group, the rat model of AMI was established by occlusion of the anterior descending branch of the left coronary artery, then electro-acupuncture therapy was performed at bilateral acupoint Neiguan （PC 6）. The myocardial enzyme activities in plasma was detected with biochemical method, and the myocardial c-fos gent expression was detected with reverse transcription polymerase chain reaction（RT PCR） method. Results： The myocardial enzyme activities in plasma and the myocardial c-fos gene expression in myocardial ischemia model group were obviously higher than those in sham group（P〈 0.05）, after electro-acupuncture treatment, the myocardial enzyme activities in plasma and the myocardial c-fos gene expression were decreased, there was a significant difference （P〈 0.05）. Conclusion. The mechanism of improvement effect of electro acupuncture at acupoint Neiguan （PC 6） on AMI is related to down-regulation of the myocardial c-fos mRNA expression.

Efficacy on rabbits with arrhythmia: needling acupoint of Neiguan(PC6) at shallow or deep depth, and retaining needles for 10, 20, or 30 min

OBJECTIVE:To compare the effects of Neiguan(PC6)acupuncture at different depths and retention time on arrhythmia duration,myocardial tissue morphology,mRNA expression level of L-type calcium channelα1C subunit and Ca^(2+)-Mg^(2+)-At Pase activity in tachirrhythmia model of rabbits.METHODS:The tachyarrhythmia model was made by intravenous injection of barium chloride into the ears of rabbits.A total of 56 healthy adult male New Zealand big-eared white rabbits,apply the random number table method,divided into normal control group(group A),model group(group B),shallow needling Neiguan(PC6)10 min group(group C),shallow needling Neiguan(PC6)20 min group(group D),shallow needling Neiguan(PC6)30 min group(group E),deep needling Neiguan(PC6)10 min group(group F),deep needling Neiguan(PC6)20 min group(group G),deep needling Neiguan(PC6)30 min group(group H),7 animals in each group.Electrocardiograms were used to collect the duration of arrhythmia;hematoxylin-eosin staining method was performed on myocardial tissue,RT-PCR tested the expression ofα1C subunit mRNA,and the activity of Ca^(2+)-Mg^(2+)-ATPase were quantified by phosphorus determination method.RESULTS:The duration of arrhythmia in each acupuncture treatment group was shortened to varying degrees.Compare to the model group,the tissue damage from barium chloride inducing was improved in the acupuncture group.Compared to the model group,except for group E,most treatment groups had varying degrees of improvement with significantly down-regulated L-type calcium channelα1C subunit mRNA expressions level and increased Ca^(2+)-Mg^(2+)-ATPase activity.CONCLUSIONS:The effect of acupuncture at Neiguan(PC6)with different depths and retention time can reduce the duration of arrhythmia induced by barium chloride relatively,improve the induced pathological changes,down regulate L-type calcium channelα1C subunit mRNA expressions level and increase Ca^(2+)-Mg^(2+)-ATPase activity.Both the shallow and deep tissues of Neiguan(PC6)may be involved in transmitting acupuncture information.There is an optimal induction period for shallow needling at Neiguan(PC6)to reach the best therapeutic effect.

Clinical Application of Acupoint Neiguan (PC 6)

Acupoint Neiguan (PC 6) is first recorded in LingShu (Spritual Pivot). It is the Luo-Connecting pointof the Pericardium Meridian and communicateswith acupoint Waiguan (TE 15); it is also one ofeight confluent points, and associates with the YinLink Vessel. We often use Neiguan (PC 6) alone oras the cardinal acupoint to treat many disorders inclinical practice. It is now reported as follows.

Effect of acupuncture at Neiguan (PC 6) on cardiac function using echocardiography in myocardial ischemia rats induced by isoproterenol

OBJECTIVE: To investigate the effect of acupuncture at Neiguan(PC 6) on cardiac function using echocardiography in rat models of myocardial ischemia(MI) induced by isoproterenol(ISO).METHODS: Twenty-seven Sprague-Dawley rats were randomly assigned to normal, model, and acupuncture groups. The model and acupuncture groups were given injections of ISO(85 mg/kg) to establish the MI model. After model establishment,the acupuncture group was treated with acupuncture at Neiguan(PC 6) for 30 min. Echocardiography was used to monitor diastolic and systolic function for 30 min starting from the time after the acupuncture needles were removed. Changes in the length of left ventricular internal diameter at end-diastole(LVIDd), length of left ventricular internal diameter at end-systole(LVIDs), the ratio of mitral peak velocity at early diastole and atrial contraction(E/A), ejection fraction(EF), fractional shortening(FS), and stroke volume(SV) were measured.RESULTS: Compared with the model group at 0and 15 min after needles were removed, the means of LVIDd and LVIDs were significantly lower(P <0.01) and E/A, EF, FS, and SV significantly higher(P < 0.01) in the acupuncture group. In the acupuncture group, the means of LVIDd and LVIDs15 min after the needles were removed were significantly higher(P < 0.01) than those at 0 min. The means of E/A, EF, FS, and SV significantly decreased(P < 0.01) from 0 to 15 min in the acupuncture group.CONCLUSION: These findings indicate that acupuncture at Neiguan(PC 6) can affect cardiac function by increasing left ventricular diastolic and systolic function in MI rat models, but the effect only lasts for 15 min.

Effect of electroacpuncture of Neiguan(PC 6)and Tianquan(PC 2)on skin temperature,blood perfusion,and adrenoceptor response in rats with acute myocardial ischemia

OBJECTIVE: To investigate the effect of electroacupuncture(EA) of acupoints Neiguan(PC 6) and Tianquan(PC 2) on the skin temperature, blood perfusion, and alpha1-and beta2-adrenoreceptor(α1-AR and β2-AR) protein and m RNA level in rats with acute myocardial ischemia.METHODS: Thirty male adult Wistar rats [(230 ±20) g] were randomly assigned into five groups(n = 6 each): a control group, sham operation group, model group, model group treated with EA at low frequency(L-EA, 2 Hz, 1 m A) and model group treated with EA at high frequency(H-EA, 100 Hz, 1 m A). The rat model was prepared by ligating the left anterior descending coronary artery. Electroacupuncture was performed at the left Neiguan(PC 6) for 20 min daily for 3 d. After the 3 rd time of the treatment, measurements of skin blood perfusion and temperature in Neiguan(PC 6) and Tianquan(PC 2) in all groups were made by laser speckle contrast imaging and infrared thermal image instrument. Real-time PCR and ELISA were used to measure m RNA level and protein level of α1-AR andβ2-AR in the skin tissues of Neiguan(PC 6) and Tianquan(PC 2), respectively. Serum levels of c Tn T and electrocardiogram were used to identify the state of myocardium.RESULTS: In the group receiving electroacupunture at Neiguan(PC 6), compared with control group,the skin temperature, blood perfusion, and β2-AR m RNA and protein level of model group significantly decreased(P < 0.05), α1-AR m RNA and protein level of model group significantly increased(P < 0.05);compared with model group, the skin temperature,blood perfusion, and β2-AR m RNA and protein level of L-EA significantly increased(P < 0.05), α1-AR m RNA and protein level of L-EA and H-EA significantly decreased(P < 0.05). The same trend has taken place in the former four groups of the Tianquan(PC 2).CONCLUSION: Low-or high-frequency electroacupuncture can improve the skin temperature and blood perfusion which may be induced by decreasingthelevelofα1-ARandincreasingthelevelofβ2-AR of the Neiguan(PC 6) and Tianquan(PC 2) in the rat with acute myocaidial ischemia.

Effect of electroacupuncture at Neiguan(PC 6) and Baihui(GV 20) on CHOP and caspase-12 gene expressions in rats after ischemia-reperfusion injury

Objective： To investigate the effects of electroacupuncture （EA） at Neiguan （PC 6） and Baihui （GV 20） by observing the changes of CCAAT/enhancer-binding protein（C/EBP） homologous protein （CHOP） and caspase-12 gene expressions in rats after cerebral ischemia-reperfusion injury （IRI）, and explore whether the apoptosis pathway of endoplasmic reticulum stress （ERS） is involved in the protective mechanisms of EA. Methods： Sixty rats were randomly assigned to five groups （12 in each group）： a normal control group （group A）, a sham-operation group （group B）, an operation group （group C）, an Edaravone group （group D） and an EA group （group E）. The cerebral IRI rat model was induced by middle cerebral artery occlusion （MCAO） using intraluminal monofilament. 2,3,5-triphenyl tetrazolium chloride （TTC） staining was adopted in the measurement of cerebral infarction volume. Real-time polymerase chain reaction （RT-PCR） was used to determine the mRNA expressions of CHOP and caspase-12. Results： Compared with group A and group B, the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group C, group D and group E were increased, with statistical significances （P〈0.05 or P〈O.01）; compared with group C, the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group D and group E were decreased significantly （P〈0.05 or P〈0.01）; there were no significant differences between group D and group E in comparing the above items （P〉0.05）. Conclusion： EA at Neiguan （PC 6） and Baihui （GV 20） can effectively suppress the volume of cerebral infarction. Furthermore, the underlying mechanism of EA at Neiguan （PC 6） and Baihui （GV 20） is possibly related to the down-regulation of CHOP and caspase-12 mRNA expressions, so as to decrease cell apoptosis.

Effect of transcutaneous electroacupuncture at Neiguan(PC 6) on refractory vomiting in patients in intensive care unit

OBJECTIVE: To observe the effect of transcutaneous electroacupuncture(TEA) at Neiguan(PC 6) on refractory vomiting in critically ill patients in intensive care(ICU) setting.METHODS: Ten patients admitted in ICU and presenting vomiting refractory to one or more antiemetic drugs were prospectively included in the study. TEA was applied at acupoint of Neiguan(PC6) during 30 min with a neuromuscular transmission monitor(single-twitch stimulation with 1 Hz at a constant current of 10 m A). Nausea and Vomiting were evaluated at the following intervals: immediately after 30 min of TEA at Neiguan(PC 6),30 min-6h and 6-24 h. The presence of nausea and/or vomiting throughout the observational period was defined as the primary end point.RESULTS: The presence of nausea or vomiting throughout the observational period was 10% at the end of TEA, 40% between 30 min and 6 h, and50% between 30 min and 24 h(P < 0.001, P = 0.01 and P = 0.03 vs pre-TEA, respectively). There were no complications or side effects related to TEA.CONCLUSION: TEA at Neiguan(PC 6) seems effective in reducing refractory vomiting in the patients in ICU setting, even if larger trials are needed to define optimal modalities.

Electroacupuncture of Neiguan(PC 6) inhibits enhanced voltage-gated sodium currents in ischemic ventricular myocytes

OBJECTIVE: To examine the effect of electroacupuncture(EA) at bilateral Neiguan(PC 6) on voltage-gated Na+currents(INa) and channels(Nav) in ischemic ventricular myocytes.METHODS: EA serum was prepared from six male adult Sprague-Dawley rats that had received EA at bilateral Neiguan(PC 6). Eighteen ventricular myocytes were prepared from six SD rats using an enzymolysis approach. Myocardial ischemia was mimicked by perfusion of ischemic solution. Whole-cell patch-clamping was used to record three currents evoked from isolated cells. The first current was the control, and recorded in absence of ischemic solution current. The second was the ischemic current,and recorded after perfusion of ischemic solution for 5 min, while the EA current was last, and recorded after perfusion of EA serum for 5 min. Navkinetic curves were fitted using related formulas.RESULTS: Compared with those in controls, in the presence of ischemic solution, peak amplitudes of INasignificantly increased from ﹣40 m V to +30 m V,and half-maximal inactivation potentials of Navincreased significantly, while half-maximal activation potentials, slope factors and the recovery time from inactivation to activation of Navwere unchanged. Compared with those in the ischemic solution, in the presence of EA serum, peak ischemic current amplitudes significantly reduced from ﹣40m V to + 40 m V, and half-maximal inactivation potentials were restored, while half-maximal activation potentials, slope factors and the recovery time from inactivation to activation of Navwere unchanged.CONCLUSION: EA at bilateral Neiguan(PC 6) can reduce enhanced INavia restoration of delayed Navinactivation in ischemic ventricular myocytes.

Influence of Transdermal Electric Stimulation of Neiguan(PC 6)On TCD in Patients with Acute Migraine

Purpose： In order to explore the clinical mechanism of Neiguan （PC 6） in the treatment of acute seizure of migraine and the relationship between Neiguan （PC 6） and cerebral blood flow. Methods： Clinically, 30 cases of the patients with acute seizure of migraine （ascending phase） were selected, in TCD abnormality, and divided randomly into the treatment group and control group, 15 cases in each group. In acute seizure of migraine, Neiguan （PC 6） was punctured with electric stimulation on the healthy side of the patients in the treatment group and Xuehai （SP 10） was punctured with electric stimulation on the healthy side of the patients in the control group. TCD was determined in the patients 30 min before and after the treatment. Results and conclusion： After transdermal electric stimulation on Neiguan （PC 6）, the velocity of intracranial blood flow was obviously slowed down, indicating that transdermal electric stimulation on Neiguan （PC 6） can effectively relieve the spasm of intracranial blood vessels and also shows its relative specificity.

Influence of acupuncture and moxibustion pretreatment at“Neiguan”( PC 6) on Connexin 43 in myocardial cell in rabbits of myocardial ischemia reperfusion

Objective To observe change of expression level of Connexin 43(Cx 43)in myocardial mitochondria of ischemia reperfusion injury after pretreatment of acupuncture and moxibustion at"Neiguan"(PC 6)in rabbits,and compare the different effect between electroacupuncture and moxibustion.Methods Thirty-two New Zealand big-eared white rabbits were randomly divided into a model group,an electroacupuncture group,a moxibustion group and a sham-operation group,8 cases in each one.The model

电针内关和神门穴对脑功能成像不同影响的观察

目的 :观察针刺内关和神门对脑功能成像的影响作用。方法 :采用功能磁共振成像技术研究电针内关和神门对不同脑区的激活状态。结果 :电针刺激内关主要引起额叶的激活 ,刺激神门主要引起颞叶的激活。结论 :针刺刺激不同的穴位 ,可以激活不同的脑区 ,电针内关和神门穴激活脑区的功能与智能有关 ,提示应用内关、神门穴治疗智能障碍有一定的客观性。

电针“内关”对心肌缺血再灌注损伤内皮素及超微结构的影响

目的 :探讨“内关”在心肌缺血再灌注损伤过程中对心肌细胞的保护作用。方法 :电针家兔左右侧“内关”穴 2 0min后 ,结扎左冠状动脉前降支 ,心电图 (ECG)监测。 4 0min后恢复灌流 ,再灌注 1小时 ,取静脉血及心肌组织 ,观察血浆内皮素 (ET)及透射电镜下组织超微结构的变化。结果 :针刺“内关”组ET含量明显降低 (P <0 0 1) ,心肌组织损伤轻微 ,肌纤维正常走向 ,少量线粒体空泡变 ,肌丝无明显坏死、溶解。结论 :电针“内关”可明显降低缺血再灌注损伤的程度 。

针刺内关穴对机体不同功能状态下fMRI脑功能成像的影响

目的:观察机体在生理或病理状态下,针刺同一穴位时fMRI脑功能成像的变化,探讨内关穴的作用机制。方法:对老年健康人(正常组)和阿尔茨海默病患者(AD组)进行电针刺激内关穴,同时运用1.5T磁共振成像系统,采集两组被试者脑部的fMRI功能像数据,通过SPM99进行统计分析,获取正常组和AD组脑内不同区域的功能活动图像。结果:正常组两侧额叶及颞叶均被不同程度激活,AD组表现在额叶、颞叶和扣带回、小脑等部位不同程度的激活,其他脑区无明显激活。结论:针刺内关穴对正常组和AD组的脑功能活动激活区域表现不同,说明针刺内关穴对机体不同功能状态下fMRI脑功能成像影响不同。