Objective:To observe the effect of acupuncture on local skin tissue metabolites of Tianshu(ST25)in rats with Crohn's disease(CD)using metabolomics and to explore the mechanism of acupuncture treatment metabolites ...Objective:To observe the effect of acupuncture on local skin tissue metabolites of Tianshu(ST25)in rats with Crohn's disease(CD)using metabolomics and to explore the mechanism of acupuncture treatment metabolites and pathway on local skin tissue of Tianshu(ST25).Materials and Methods:Thirty-eight Sprague-Dawley rats were randomly assigned to a normal group(n=14)and a CD modeling group(n=24).Rats in the CD modeling group were administered with 2,4,6-trinitrobenzene sulfonic acid and ethanol enema,in addition to the normal group.Four normal rats and four CD modeling rats were selected at random after model identification was established.Furthermore,CD modeling group rats were randomly assigned to two groups of 10 rats:the model group and acupuncture group.Rats in the acupuncture group underwent acupuncture of Tianshu(ST25)and Shangjuxu(ST37)for 7 days.After the intervention,the colon tissue was collected from each group of rats and the pathological changes were observed through hematoxylin and eosin(HE)staining.The local skin tissues at Tianshu(ST25)of the rats in each group were taken,and the proton nuclear magnetic resonance technique was used to detect the metabonomics of the local skin tissues of Tianshu(ST25)in rats.Results:Compared with the normal group,the following were observed in the model group after HE staining:irregular colon morphology and other pathological changes such as intestinal mucosal hyperemia,edema,ulcers,polyps,and inflammatory cell infiltration.In the acupuncture group,colon tissue structure was relatively complete and layered,the colon gland structure was restored,and inflammatory cell infiltration was significantly improved.The choline,glycerin,glycine,guanidoacetic acid,and proline levels were significantly higher in the model group than in the normal group(P<0.05 or P<0.01).Compared with the model group,the acupuncture group had significantly increased contents of alanine,leucine,L-phenylalanine,and tyrosine in the skin(P<0.05 or P<0.01).Valine,leucine,and L-isoleucine biosynthesis and L-phenylalanine metabolism were the main metabolic pathways involved in the changes in the local skin tissues of the rats in each group,biosynthesis of tyrosine,and tryptophan.Conclusion:The change in the metabolites in the local area of Tianshu(ST25)may be related to intestinal disease.The acupuncture of Tianshu(ST25)can improve intestinal inflammatory reaction in rats with CD,and this finding may be related to the regulation of amino acid metabolites and their pathways in the local acupoint tissues of Tianshu(ST25)by acupuncture.展开更多
目的:观察电针天枢、上巨虚穴对结肠炎大鼠肠黏膜中维生素D受体(VDR)表达的影响,探讨电针治疗溃疡性结肠炎(UC)的可能机制。方法:按照随机数字表法将雄性Wistar大鼠分为对照组、模型组和电针组,每组8只。采用三硝基苯磺酸(TNBS)灌肠的...目的:观察电针天枢、上巨虚穴对结肠炎大鼠肠黏膜中维生素D受体(VDR)表达的影响,探讨电针治疗溃疡性结肠炎(UC)的可能机制。方法:按照随机数字表法将雄性Wistar大鼠分为对照组、模型组和电针组,每组8只。采用三硝基苯磺酸(TNBS)灌肠的方法来诱导溃疡性结肠炎制备大鼠模型,同时采用大鼠疾病活动指数(DAI)评分和组织学活动度评分(HAI)来评估结肠炎症程度。电针组大鼠经TNBS造模成功后次日取天枢、上巨虚穴进行电针治疗,共14 d。于第15天处死大鼠后收集结肠组织,分别采用免疫组化和实时荧光定量PCR法(qRT-PCR)检测肠黏膜VDR的表达水平,采用分光光度法测定结肠组织髓过氧化物酶(MPO)活性。结果:与模型组比较,电针组HAI明显下降(P=0.005)。与对照组比较,模型组和电针组结肠组织中MPO活性均明显增加。与模型组比较,电针组结肠组织中MPO活性下降(P<0.001)。与对照组比较,模型组、电针组大鼠肠黏膜中VDR mRNA及蛋白表达降低(P<0.01)。与模型组比较,电针组大鼠肠黏膜VDR m RNA及蛋白表达升高(P<0.05)。蛋白免疫组化提示VDR主要在上皮细胞中表达。VDR蛋白表达水平与MPO活性成负相关(P<0.05)。结论:电针天枢、上巨虚两穴可能通过上调肠黏膜VDR表达来缓解大鼠UC症状。展开更多
OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine (DA) and dopamine r...OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine (DA) and dopamine receptor 1 (D1 R) in the colon,spinal dorsal horn and hypothalamus.METHODS:The rats were randomly divided into the normal,model (CD),herb-partitioned moxibustion (Mox) and mesalazine (Mesa) groups.Damage in the colons was scored and observed by hematoxylin and eosin staining.DA and D1R protein expression in the colonic mucosa were detected by immunohistochemistry.The concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay,and D1R mRNA expression was evaluated by quantitative real-time polymerase chain reaction.RESULTS:In the colon,compared with the normal group,DA,D1 R protein expressions and D1 R mRNA expression were significantly higher in the model group,while decreased in the Mox group and the Mesa group.In the spinal dorsal horn and hypothalamus,compared with the normal group,the concentrations of DA and D1 R,and the D1R mRNA expressions were significantly higher in the model group,and decreased in the Mox group and the Mesa group.CONCLUSION:Herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints relieved ulceration in CD rats,the underlying mechanism maybe relative with the regulation of DA and D1R in the colon,spinal dorsal horn and hypothalamus by moxibustion.展开更多
基金National Natural Science Foundation of China(No.81603705,No.81774438)China Postdoctoral Science Foundation Project(No.2107M612567)+4 种基金Natural Science Foundation of Hunan Province(No.2021JJ30513,No.2017JJ3245)Scientific Research Project of Hunan Provincial Department of Education(No.21A0235,No.20B444)Hunan Traditional Chinese Medicine Research Project(No.C2022027)Hunan Province Youth Backbone Teacher Training Target Project(2021)Changsha Natural Science Foundation(No.kq2208183).
文摘Objective:To observe the effect of acupuncture on local skin tissue metabolites of Tianshu(ST25)in rats with Crohn's disease(CD)using metabolomics and to explore the mechanism of acupuncture treatment metabolites and pathway on local skin tissue of Tianshu(ST25).Materials and Methods:Thirty-eight Sprague-Dawley rats were randomly assigned to a normal group(n=14)and a CD modeling group(n=24).Rats in the CD modeling group were administered with 2,4,6-trinitrobenzene sulfonic acid and ethanol enema,in addition to the normal group.Four normal rats and four CD modeling rats were selected at random after model identification was established.Furthermore,CD modeling group rats were randomly assigned to two groups of 10 rats:the model group and acupuncture group.Rats in the acupuncture group underwent acupuncture of Tianshu(ST25)and Shangjuxu(ST37)for 7 days.After the intervention,the colon tissue was collected from each group of rats and the pathological changes were observed through hematoxylin and eosin(HE)staining.The local skin tissues at Tianshu(ST25)of the rats in each group were taken,and the proton nuclear magnetic resonance technique was used to detect the metabonomics of the local skin tissues of Tianshu(ST25)in rats.Results:Compared with the normal group,the following were observed in the model group after HE staining:irregular colon morphology and other pathological changes such as intestinal mucosal hyperemia,edema,ulcers,polyps,and inflammatory cell infiltration.In the acupuncture group,colon tissue structure was relatively complete and layered,the colon gland structure was restored,and inflammatory cell infiltration was significantly improved.The choline,glycerin,glycine,guanidoacetic acid,and proline levels were significantly higher in the model group than in the normal group(P<0.05 or P<0.01).Compared with the model group,the acupuncture group had significantly increased contents of alanine,leucine,L-phenylalanine,and tyrosine in the skin(P<0.05 or P<0.01).Valine,leucine,and L-isoleucine biosynthesis and L-phenylalanine metabolism were the main metabolic pathways involved in the changes in the local skin tissues of the rats in each group,biosynthesis of tyrosine,and tryptophan.Conclusion:The change in the metabolites in the local area of Tianshu(ST25)may be related to intestinal disease.The acupuncture of Tianshu(ST25)can improve intestinal inflammatory reaction in rats with CD,and this finding may be related to the regulation of amino acid metabolites and their pathways in the local acupoint tissues of Tianshu(ST25)by acupuncture.
文摘目的:观察电针天枢、上巨虚穴对结肠炎大鼠肠黏膜中维生素D受体(VDR)表达的影响,探讨电针治疗溃疡性结肠炎(UC)的可能机制。方法:按照随机数字表法将雄性Wistar大鼠分为对照组、模型组和电针组,每组8只。采用三硝基苯磺酸(TNBS)灌肠的方法来诱导溃疡性结肠炎制备大鼠模型,同时采用大鼠疾病活动指数(DAI)评分和组织学活动度评分(HAI)来评估结肠炎症程度。电针组大鼠经TNBS造模成功后次日取天枢、上巨虚穴进行电针治疗,共14 d。于第15天处死大鼠后收集结肠组织,分别采用免疫组化和实时荧光定量PCR法(qRT-PCR)检测肠黏膜VDR的表达水平,采用分光光度法测定结肠组织髓过氧化物酶(MPO)活性。结果:与模型组比较,电针组HAI明显下降(P=0.005)。与对照组比较,模型组和电针组结肠组织中MPO活性均明显增加。与模型组比较,电针组结肠组织中MPO活性下降(P<0.001)。与对照组比较,模型组、电针组大鼠肠黏膜中VDR mRNA及蛋白表达降低(P<0.01)。与模型组比较,电针组大鼠肠黏膜VDR m RNA及蛋白表达升高(P<0.05)。蛋白免疫组化提示VDR主要在上皮细胞中表达。VDR蛋白表达水平与MPO活性成负相关(P<0.05)。结论:电针天枢、上巨虚两穴可能通过上调肠黏膜VDR表达来缓解大鼠UC症状。
基金Supported by the National Basic Research Program of China(973 Program,No.2015CB554501)Program of National Natural Science Foundation of China(No.81574079)+2 种基金Program for outstanding medical academic leader(2015,No.80)Shuguang Program of Shanghai Education Commission(No.14SG39)Shanghai Rising-Star Program(No.16QA1403400)
文摘OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine (DA) and dopamine receptor 1 (D1 R) in the colon,spinal dorsal horn and hypothalamus.METHODS:The rats were randomly divided into the normal,model (CD),herb-partitioned moxibustion (Mox) and mesalazine (Mesa) groups.Damage in the colons was scored and observed by hematoxylin and eosin staining.DA and D1R protein expression in the colonic mucosa were detected by immunohistochemistry.The concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay,and D1R mRNA expression was evaluated by quantitative real-time polymerase chain reaction.RESULTS:In the colon,compared with the normal group,DA,D1 R protein expressions and D1 R mRNA expression were significantly higher in the model group,while decreased in the Mox group and the Mesa group.In the spinal dorsal horn and hypothalamus,compared with the normal group,the concentrations of DA and D1 R,and the D1R mRNA expressions were significantly higher in the model group,and decreased in the Mox group and the Mesa group.CONCLUSION:Herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints relieved ulceration in CD rats,the underlying mechanism maybe relative with the regulation of DA and D1R in the colon,spinal dorsal horn and hypothalamus by moxibustion.
