Effect of electroacupuncture at Jiaji (EX-B2) points on the expression of microRNA-21 and neuronal apoptosis in rats with acute spinal cord injury

Objective:To observe the effect of electroacupuncture at Jiaji (EX-B2) points on the expression of microRNA-21 and neuronal apoptosis in rats with acute spinal cord injury. Methods:SD rats were randomly divided into sham-operated group, model group, electro-acupuncture group and methylprednisolone group. The model of acute spinal cord injury was reproduced by modified Allen's method. Each group was given corresponding intervention treatment 2 hours after modeling. The electroacupuncture group was treated with electro-acupuncture at Jiaji (EX-B2) points in T8 to T12, the methylprednisolone group was given intraperitoneal injection of 30 mg/kg methylprednisolone sodium succinate, and the sham operation group and model group were not treated. After treatment, BBB scores were observed to evaluate the motor function of posterior limb in each group, and the injured spinal cord tissues were taken. The pathological changes of spinal cord neuropathy were observed by Nissl staining. The expression of microRNA-21 was detected by RT-qPCR, and the expression of apoptotic proteins (Bax, Bcl-2 and cleaved-Caspase-3) was detected by Western-Blot. Result:Compared with sham operation group, BBB, neuron survival rate and expression of microRNA-21 were significantly lower, apoptotic rate of neurons was significantly higher, expression of Bax, Bcl-2, cleaved Caspase-3 protein and Bax/Bcl-2 ratio were significantly increased in model group (P< 0.05). Electroacupuncture could significantly increase BBB score, neuron survival rate and expression of microRNA-21, reduce apoptotic rate of neurons and the ratio of Bax/Bcl-2, and inhibit the expression of Bax, Bcl-2 and cleaved Caspase-3 protein in tissues, which were significantly different from those of model group (P< 0.05). Conclusions: Electroacupuncture at Jiaji (EX-B2) points can significantly promote the recovery of neurological function in rats after ASCI, and its mechanism may be closely related to the up-regulation of the expression of microRNA-21 in tissues and the inhibition of the activation of Bax/Bcl-2/cleaved Caspase-3 signaling pathway.

THE TREATMENT OF CERVICAL SPONDYLOPATHY BY LASER ACUPUNCTURE AT THE CERVICAL JIAJI POINTS: AN ANALYSIS OF THERAPEUTIC EFFECT OF 102 CASES

Laser acupuncture therapy was employed in the treatment of spondylopathy.Thetherapeutic effect of the three types of the spondylopathy,nerve root type,vertebral artery type andspinal cord type were stuied and compared.The results indicate a significant difference between thethree types of spondylopathy.The best therapeutic effect was obtained in nerve root type with a remarkable effective rate of 98.5％;the effective rates for the vertebral artery type and the spinal cordtype were 92.3％and 25％,respectively.Combining laser irradiatlon with acupuncture,the therapyposseses the functions of acupuncture,heat effect and mild moxibustion action.Therefore,it showedeffect quickly and had the advantages of short treatment course and high therapeutic effect.Clinicalobservation has confirmed that laser acupuncture is more effective than single laser irradiation.Thistherapy offers another simple but effective method for the treatment of spondylopathy.

Effectiveness of electroacupuncture at Jiaji acupoints(EX-B 2), plus moxibustion and intermediate on postherpetic neuralgia: a randomized controlled trial

OBJECTIVE:To investigate the effectiveness of electroacupuncture at Jiaji acupoints(EX-B 2)plus moxibustion and intermediate frequency on postherpetic neuralgia(PHN).METHODS:A total of 140 outpatients who satisfied the inclusion criteria and volunteered for this treatment were randomly divided into treatment(n=70)and control(n=70)groups.Both groups received a localized lesion area and electroacupuncture treatment combined with moxibustion and intermediate frequency.The treatment group(TG)increased acupuncture at Jiaji acupoints(EX-B 2)and electroacupuncture.Pain and anxiety were assessed before and after 5,10,15,and 20 treatments by using visual pain simulation score(VAS)and Hamilton anxiety scale(HAMA),respectively.Clinical efficacy was also evaluated.RESULTS:The baseline between the two groups did not significantly differ(P>0.05).The VAS and HAMA scores of the two groups after treatment significantly decreased compared with those of various treatment stages(P>0.05).The HAMA score(P<0.01)of TG was lower than that of the control group(CG).The VAS score of TG was lower than that of CG in the 5th and 10th treatments(P<0.01).In the 15 th and 10 th scores,CG was also superior to TG(P<0.05).CONCLUSION:The combined treatment of electroacupuncture at Jiaji acupoints(EX-B 2),moxibustion,and intermediate frequency can relieve the pain and anxiety symptoms of PHN.The efficacy of the combined treatment was superior to traditional acupuncture.

Clinical observation on acupuncture at Jiaji(EX-B 2) points plus tuina in treating thoracic facet joint disorder

Objective： To observe the clinical effects of acupuncture at Jiaji （EX-B 2） points plus tuina manipulation for thoracic facet joint disorder, and get new clinical evidence for treatment of thoracic facet joint disorder. Methods： Totally 106 patients with thoracic facet joint disorder were randomly allocated into an observation group and a control group based on the random number table. Patients in the control group were treated by tuina manipulation, while those in the observation group were treated by acupuncture at Jiaji （EX-B 2） points before tuina manipulation. Patients in the two groups were treated once a day. The improvements of signs and symptoms and the efficacy were observed after 3 treatments. Results： After treatment, there were intra-group statistical differences in scores of 8 signs and symptoms in both groups （P〈0.01}; the score of each item in the observation group was lower than that in the control group, and there was statistical significance in the inter-group difference （P〈0.05）. The cure rate of the observation group was 50.9%, versus 26.4% in the control group, and there was a significant difference between the two groups （P〈0.05）. Conclusion： In treating thoracic facet joint disorder, acupuncture plus tuina manipulation can restore the biomechanical balance of thoracic vertebrae, fully maximized the combined effect, and significantly improve the clinical efficacy.

Acupuncture-moxibustion at Jiaji(EX-B 2) points for intervertebral disc herniation: a systematic review

By organizing and classifying the literatures related to acupuncture-moxibustion at Jiaji （EX-B 2） points in treating intervertebral disc herniation （IDH） published before December 32, 2013, to summarize the application status and features of Jiaji （EX-B 2） points. It＇s found that its most favorable indication is IDH. It also further analyzed the action mechanism of acupuncture-moxibustion at Jiaji （EX-B 2） points in treating IDH, and brought up some ideas to highlight the further study.

Observation on Therapeutic Effects of Electroacupuncture at Jiaji (Ex-B 2) Points plus Herbal Medicine for Herpes Zoster

Objective： To observe the clinical effects of electroacupuncture （EA） at Jiaji （Ex-B 2） points plus herbal medicine for herpes zoster （HZ）. Methods： Sixty-two cases were randomly divided into a treatment group and a control group. The treatment group was treated with EA at Jiaji （Ex-B 2） points plus herbal medicine based upon the pattern differentiation. The control group was treated with oral administration of Aciclovir tablets for resisting virus, and Mecobalamin tablets for nourishing the nerves and Aciclovir cream for skin rash. The therapeutic effects were compared between the two groups, after two-week treatment Results： After 7 d of the treatment, the therapeutic effect was remarkably better in the treatment group than that in the control group （P〈0.01）. After two-week treatment, the therapeutic effects were still different between the two groups （P〈0.05）. Conclusion： The therapeutic effect of EA at Jiaji （Ex-B 2） points plus herbal medicine for HZ is better than that of Western medications in resisting virus and nourishing nerves.

Intractable post-herpetic neuralgia in the trunk treated with point injection at Jiájǐ(夹脊 EX-B 2)

ABSTRACT Objective To compare the efficacy difference between point injection at Jiiaji （夹脊 EX-B 2） and oral medication in the treatment of intractable post-herpetic neuralgia （PHN） in the trunk. Methods One hundred and thirty cases were randomly divided into an point injection group and an medication group, 65 cases in each one. In point injection group, according to the invasion site of herpes zoster, Jiiaji （夹脊 EX-B 2） of corresponding segments and the meridian points on skin lesion areas, such as Fengchi （风池GB 20）, Tianzhu （天柱 BL 10）, Feishu （肺俞 BL 13） and Geguan （膈关 BL 46） were selected and injected with the mixed solution of compound Betamethasone （administered only in the first injection）, Vitamin B12 and Lidoeaine Carbonate, 2 mL on each point, once per day. In medication group, Diclofenac Sodium sustained release tablets, 75 mg were administered, twice per day. The clinical efficacy was compared 10 days later between two groups. Results The total effective rate in point injection group was 100.0% （65/65）, which was better than 66.1% （43/65） in medication group （P〈0.01）. Conclusion Point injection mainly at Jiiaji （夹脊 EX-B 2） is effective significantly on intractable PHN in the trunk, which is superior to Diclofenac Sodium sustained release tablets.

电针夹脊穴治疗脊髓损伤机制的实验研究进展

通过对近年来电针夹脊穴治疗脊髓损伤(spinal cord injury,SCI)相关实验研究文献进行整理和分析,从改善神经修复的微环境、抑制细胞凋亡和促进神经修复三方面论述电针夹脊穴治疗SCI的机制,并尝试探讨其未来可能的发展方向。

颈夹脊穴注射合颈神经根阻滞治疗神经根型颈椎病临床观察

目的观察颈夹脊穴注射复方当归注射液联合颈神经根阻滞治疗神经根型颈椎病的临床疗效。方法将96例神经根型颈椎病患者随机分为3组,A组(32例)行颈夹脊穴注射当归联合颈神经根阻滞治疗,B组(32例)单行颈神经根阻滞治疗,C组(32例)给予常规方法治疗,包括口服消炎镇痛药物、神经营养药物。治疗后1星期、3星期、3个月对3组疗效进行随访。结果 3组治疗后1星期、3星期、3个月VAS值较治疗前明显降低(P<0.01);与C组比较,A组和B组各时间点的VAS评分明显降低(P<0.05);但是治疗后3个月的VAS评分,A组明显低于B组(P<0.05)。3组治疗后3个月改良Macnab疗效差异有统计学意义(P<0.05),有效率分别为96.9%,87.5%,75.0%,根据卡方检验的结果,A组疗效优于B组和C组。3组之间治疗前后CGRP水平的差异有统计学意义(P<0.01),认为A组、B组、C组在治疗后3个月外周血CGRP水平均有所下降,但A组下降较B组明显,B组下降较C组明显。结论治疗神经根型颈椎病,单用常规药物治疗虽然有效,但是起效慢,显效率不高,不是最优化的治疗方法;单行颈神经阻滞治疗神经根型颈椎病与颈夹脊穴注射当归联合颈神经根阻滞治疗神经根型颈椎病起效快,显效率高,但后者的远期疗效优于前者。

Spinal stenosis treated with acupuncture on Huátuó Jiájǐ(夹脊EX-B 2) and vertebral curvature adjustment:report of therapeutic effect on 189 cases

Objective To observe the clinical effects on spinal stenosis treated with acupuncture on Huatuo Jiaji （夹脊EX-B 2） and vertebral curvature adjustment. Methods Comprehensive therapy was applied to all of 189 cases of any type of spinal stenosis, in which gukong needling technique was applied to Huatuo Jiaji （夹脊EX-B 2） at C4 - T5 and T12 - L5, associated with vertebral curvature adjustment with traction. The therapeutic effects were observed. Results Total effective rate was 96.8%, in which, clinical cured rate was 31.7% and excellent and good rate was 89.9% in average 28-month fol- low-up visit. Conclusion Spinal stenosis is segmental dynamic stenosis. It can be cured by acupuncture on Huatuo Jiaji （夹脊EX-B 2） and vertebral curvature adjustment and satisfactory result is achieved.

温灸对气血不足型椎动脉型颈椎病血液流变学的影响

目的观察温灸对气血不足型椎动脉型颈椎病血液流变学的影响。方法将确诊为气血不足型椎动脉型颈椎病患者60例,按照就诊先后顺序随机分为治疗组和对照组,每组30例,对照组采用针刺颈夹脊穴常规治疗,治疗组在此基础上加温灸,治疗前后均做TCD检查,观察两组血液流变学的变化,并判断临床疗效。结果治疗组总有效率为96.7%,对照组总有效率为90.0%。两组总有效率比较差异有统计学意义(P<0.05),治疗前后TCD检测均有差异(P<0.05),治疗组能更好改善椎基底动脉的血流状况(P<0.05)。结论温灸能较好地改善椎动脉型颈椎病的椎基底动脉的血流状况,是治疗椎动脉型颈椎病的临床有效方法。

针刺华佗夹脊穴对脑卒中后肢体功能障碍的改善作用观察

【目的】评价针刺华佗夹脊穴疗法改善脑卒中后肢体功能障碍的临床疗效。【方法】将64例缺血性脑卒中患者随机分为治疗组和对照组,每组各32例。治疗组采用华佗夹脊穴联合常规康复方法治疗,对照组采用手足十二针为主联合常规康复方法治疗,每日1次,每周治疗6次,2周为1个疗程,共治疗4个疗程。观察2组患者治疗前后肌张力评分、运动功能评分和日常生活活动能力评分,并评价其临床疗效。【结果】(1)研究过程中,治疗组和对照组各脱落2例。最终治疗组和对照组各30例纳入疗效统计。(2)治疗4周和8周后,2组患者的肌张力评分均明显改善(P<0.05),且治疗组的改善作用明显优于对照组(P<0.05)。(3)治疗4周和8周后,2组患者的运动功能评分均明显改善(P<0.05),且治疗组的改善作用明显优于对照组(P<0.05)。(4)治疗4周和8周后,2组患者的日常生活活动能力评分均明显改善(P<0.05),且治疗组的改善作用明显优于对照组(P<0.05)。(5)治疗组总有效率为86.70%,对照组为60.00%。治疗组疗效优于对照组,差异有统计学意义(P<0.05)。(6)试验过程中,2组患者均未见明显不良反应。【结论】针刺华佗夹脊穴改善脑卒中后肢体功能障碍的临床效果明显。

温针灸华佗夹脊穴治疗亚健康临床观察

目的由于亚健康缺乏特异性治疗,该文以温针灸华佗夹脊穴的方法观察治疗亚健康的疗效。方法将60例亚健康患者随机分为治疗组和对照组,治疗组取华佗夹脊穴温针灸治疗,对照组采用传统穴位温针灸治疗。观察两组临床疗效及治疗前后临床症状积分改善情况。结果治疗组疗效明显优于对照组(P<0.05)。两组治疗后临床症状积分均有下降(P<0.05),但治疗组下降更明显(P<0.05)。结论相对传统取穴温针灸治疗,温针灸华佗夹脊治疗亚健康有独特的优势。

穴位注射配合药物治疗带状疱疹后遗神经痛的临床研究

目的观察穴位注射配合药物治疗带状疱疹后遗神经痛的临床疗效。方法将64例带状疱疹后遗神经痛患者随机分为治疗组和对照组,每组32例。治疗组采用夹脊穴注射丹参注射液配合口服加巴喷丁片治疗,对照组采用单纯口服加巴喷丁片治疗。两组如疼痛严重可加服盐酸曲马多缓释片治疗。观察两组治疗前后视觉模拟评分法(visual analogue scale, VAS)评分、SF-36健康量表(the MOS 36-item short-form health survey,SF-36)各项评分的变化情况,并比较两组不同时间点服用加巴喷丁片和盐酸曲马多缓释片的剂量及各项不良反应的发生率。结果两组治疗后不同时间点(治疗1周、3周、6周、12周、24周后)VAS评分与同组治疗前比较,差异均有统计学意义(P<0.01)。治疗组治疗后不同时间点VAS评分与对照组比较,差异具有统计学意义(P<0.01)。两组治疗3周、6周、12周、24周后加巴喷丁片和盐酸曲马多缓释片的服用剂量比较,差异均具有统计学意义(P<0.05)。两组治疗12周、24周后SF-36各项评分(PF、RP、BP、GH、VT、SF、RE、MH)与同组治疗前比较,差异均具有统计学意义(P<0.05)。治疗组治疗12周、24周后SF-36中PF、RP、BP、VT、MH评分与对照组比较,差异均具有统计学意义(P<0.05)。治疗组治疗后头晕、嗜睡、恶心的发生率分别为6.7%、3.3%和3.3%,对照组分别为24.1%、17.2%和20.7%,两组比较差异均具有统计学意义(P<0.05)。结论穴位注射配合药物是一种治疗带状疱疹后遗神经痛的有效方法,能减少服用加巴喷丁片的剂量,从而减少不良反应。

头针结合电针治疗腰椎间盘突出症疗效观察

目的观察头针结合电针夹脊穴治疗腰椎间盘突出症的临床疗效。方法选取腰椎间盘突出症患者86例,随机分为观察组和对照组,对照组予以电针夹脊穴治疗,观察组予以头针结合电针夹脊穴治疗,采用日本骨科学会(JOA)制定的腰椎间盘突出症的评分标准作为评价指标,2个疗程后对比两组患者的临床疗效。结果两组患者治疗前后组内5个项目症状积分比较差异均有统计学意义(P<0.01);两组患者治疗后组间5个项目症状积分比较差异均有统计学意义(P<0.05);治疗后观察组优良率为88.4%,对照组优良率为69.8%,观察组优于对照组(P<0.05)。结论头针结合电针夹脊穴治疗腰椎间盘突出症优良率优于电针夹脊穴治疗。

华佗夹脊穴理论构建的认识与启示

华佗夹脊穴作为以古代解剖知识建立起来的代表性理论,反映了华佗一派的学术思想。通过对针灸经典理论和文史资料的挖掘,认为五体是华佗夹脊穴的结构基础,血气是其物质基础,标本、气街是其功能体现;华佗夹脊穴揭示了经穴横向分布与主治相关的规律,为针灸治疗提供了理论依据;强调熟悉解剖知识对针灸疗效的重要性,提出了建立有效的经穴分类体系的新思路。

Tuina plus Cupping Therapy for 60 Cases of Infantile Cough

Objective： To observe the clinical effect oftuina plus cupping therapy for infantile cough. Methods： Sixty subjects were mainly treated with pinching and pushing the spine, coordinating with the tuina manipulations and cupping therapy based on syndrome differentiation. Results： Among the 60 patients, 40 cases were cured and 20 cases got improved, the total effective rate was 100%. Conclusion： Tuina plus cupping therapy works effectively on infantile cough and without adverse reactions.

Application of Nape Acupuncture in Treatment of Vertebral-artery Type Cervical Spondylosis

Objective： To observe the effect of nape acupuncture on vertebral-artery type cervical spondylosis. Methods： Eighty patients with vertebral-artery type cervical spondylosis were randomly divided into nape acupuncture group and Jiaji acupuncture group, with 40 cases in each group. The patients in the nape acupuncture group were treated with the nape acupuncture plus Jiaji acupuncture, while the patients in the Jiaji group were treated with Jiaji acupuncture only, to observe the functional scale before and after treatment. Results： The total effective rate in the nape acupuncture group was 95.0%, while the total effective rate in the Jiaji acupuncture group was 82.5%. By Ridit analysis, u=5.186, P〈0.01. It indicated that the therapeutic effect in the nape acupuncture group was better than that in the Jiaji acupuncture group. Compared with Jiaji acupuncture group, the difference value of pre- and post-treatment about functional scale of vertebral-artery type cervical spondylosis and the post-treatment score have statistic differences （P〈0.01）. It indicated that the therapeutic effect of nape acupuncture group was more obvious in treating vertigo than that in the Jiaji acupuncture group. Conclusion： The treatment of vertebral-artery type cervical spondylosis by Jiaji acupuncture plus the nape acupuncture may enhance the therapeutic effect significantly.

Treatment of Herpes Zoster with Electroacupuncture plus Collateral-pricking and Cupping Therapy

Objective： To observe the clinical effect of electroacupuncture （EA） plus collateral-pricking and cupping therapy for herpes zoster. Methods： Fifty-three cases with herpes zoster were randomly divided into two groups. Thirty-one cases in the treatment group were treated with EA at Jiaji （EX-B 2） points plus local collateral-pricking and cupping therapy. Twenty-two cases in the control group were treated with oral administration of VaLacido~;ir Hvdrochloride and Indomethaci~. Results： The curative and remarkable effective rate was 93.5% in the treatment group and 72.7% in the control group. The difference between the two groups was statistically significant （P〈0.01）, indicating that EA plus collateral-pricking and cupping therapy is better than Western medications in the clinical effects for herpes zoster. Conclusion： The EA plus collateral-pricking and cupping method is an effective therapy for herpes zoster.

Clinical observation of warm needling moxibustion for rheumatoid arthritis

Objective： To observe the clinical efficacy of warm needling moxibustion at points on the back in treating rheumatoid arthritis （RA）. Methods： Sixty RA patients were randomized into two groups by the random number table, 30 in each group. The observation group was intervened by warm needling moxibustion at the points from the Governor Vessel on the back and Jiaji （EX-B 2） points, while the control group was by regular acupuncture. Results： After intervention, the morning stiffness, joint pain index, joint swelling index and erythrocyte sedimentation rate （ESR） were significantly improved in both groups （P〈0.01）; the inter-group difference was also statistically significant （P〈0.05）. The total effective rate was 93.3% in the observation group versus 76.6% in the control group, and the difference was statistically significant （P〈0.01）. Conclusion： Warm needling moxibustion at points from the Governor Vessel on the back and Jiaji （EX-B 2） points can produce a higher efficacy than regular acupuncture in treating RA.