Objective:To observe the clinical efficacy of point application with De Huang(Rhizome Rhei Crude)powder at Shenque(CV 8)plus moxa-salt hot compress on the umbilicus for preventing gastrointestinal adverse reactions af...Objective:To observe the clinical efficacy of point application with De Huang(Rhizome Rhei Crude)powder at Shenque(CV 8)plus moxa-salt hot compress on the umbilicus for preventing gastrointestinal adverse reactions after chemotherapy for non-Hodgkin lymphoma(NHL).Methods:A total of 60 cases with NHL under chemotherapy were divided into two groups by hospitalization order,with 30 cases in each group.The control group was treated with routine nursing and the observation group was additionally given point application with Da Hueng(Rhizome Rhei Crude)powder plus moxa-salt hot compress on the umbilicus,to compare the effect in preventing gastrointestinal adverse reactions after chemotherapy between the two groups.Results:The occurrence rates of constipation,nausea,vomiting and poor appetite on the second day and fifth day after chemotherapy were obviously lower in the observation group than those in the control group,with statistically significant differences between the two groups(all P〈0.05).Conclusion;The point application with De Hueng(Rhizome Rhei Crude)powder at Shenque(CV 8)plus maxa-salt hot compress on the umbilicus can produce more significant efficacy in preventing the gastrointestinal adverse reactions after chemotherapy for NHL than routine nursing.Moreover,it is simple and easy to be used and popularized.展开更多
Objective: To observe the treatment effect of acupoint sticking at Shenque (CV 8) with ginger-preparedBan Xia (Rhizoma Pinelliae) on nausea and vomiting induced by Amifostine for myelodysplastic syndromes (MDS)...Objective: To observe the treatment effect of acupoint sticking at Shenque (CV 8) with ginger-preparedBan Xia (Rhizoma Pinelliae) on nausea and vomiting induced by Amifostine for myelodysplastic syndromes (MDS). Methods: Totally 124 eligible subjects intervened by Amifostine were randomized into 2 groups by the visiting order,an observation group and a control group,62 in each group. The control group was intervened by conventional treatment, while the observation group was by acupoint sticking at Shenque (CV 8) with ginger-preparedBan Xia (Rhizoma Pinelliae) in addition to the same conventional treatment. The occurrence rate of nausea and vomiting in the two groups were observed. Results: After intervention, the occurrence rate of nausea and vomiting in the observation group was significantly lower than that in the control group (P<0.01). Conclusion: Acupoint sticking at Shenque (CV 8) with ginger-prepared Ban Xia (Rhizoma Pinelliae)can produce a content effect on nausea and vomiting induced by Amifostine for MDS.展开更多
Background:Shenque(CV8)acupoint is located on the navel and has been therapeutically used for more than 2000 years in Traditional Chinese Medicine(TCM).However,clinical research on the underlying therapeutic molecular...Background:Shenque(CV8)acupoint is located on the navel and has been therapeutically used for more than 2000 years in Traditional Chinese Medicine(TCM).However,clinical research on the underlying therapeutic molecular mechanisms of the CV8 acupoint lags far behind.This study aimed to study the mechanisms of umbilical acupoint therapy by using stem cells.Methods:The morphological characteristics of CV8 acupoint were detected under a stereomicroscope using hematoxylin and eosin(H&E)staining.Oil Red,Masson,and immunohistochemical staining on multi-layered slices were used to identify the type of cells at the CV8 acupoint.Cell proliferation was measured by a cell counting kit-8(CCK-8)method.Flow cytometry and immunohistochemistry were used for cell identification.Induced differentiation was used to compare the differentiation of cells derived from CV8 acupoint and non-acupoint somatic stem cells into other cell types,such as osteogenic,adipogenic,and neural stem cell-like cells.Results:Morphological observations showed that adipose tissues at the linea alba of the CV8 acupoint in mice had a mass-like distribution.Immunohistochemical staining confirmed the distribution of stem cell antigen-1(Sca-1)positive cells in the multi-layered slices of CV8 acupoint tissues.Cells isolated from adipose tissues at the CV8 acupoint exhibited high expression of Sca-1 and CD44 and low expression of CD31 and CD34,and these cells possessed osteogenic,adipogenic,and neurogenic stem cell-like cell differentiation ability.The cell proliferation(day 4:0.5138±0.0111 vs.0.4107±0.0180,t=8.447,P=0.0011;day 5:0.6890±0.0070 vs.0.5520±0.0118,t=17.310,P<0.0001;day 6:0.7320±0.0090 vs.0.6157±0.0123,t=13.190,P=0.0002;and day 7:0.7550±0.0050 vs.0.6313±0.0051,t=42.560,P<0.0001),adipogenic([9.224±0.345]%vs.[3.933±1.800]%,t=5.000,P=0.0075),and neurogenic stem cell-like cell differentiation(diameter<50 mm:7.2000±1.3040 vs.2.6000±0.5477,t=7.273,P<0.0001;diameter 50–100 mm:2.6000±0.5477 vs.1.0000±0.7071,t=4.000,P=0.0039;and diameter>100 mm:2.6000±0.5477 vs.0.8000±0.8367,t=4.025,P=0.0038)were significantly enhanced in somatic stem cells derived from the CV8 acupoint compared to somatic stem cells from the groin non-acupoint.However,cells possessed significantly weaker osteogenicity([2.697±0.627]%vs.[7.254±0.958]%,t=6.893,P=0.0023)in the CV8 acupoint group.Conclusions:Our study showed that CV8 acupoint was rich with adipose tissues that contained abundant somatic stem cells.The biological examination of somatic stem cells derived from the CV8 acupoint provided novel insights for future research on the mechanisms of umbilical therapy.展开更多
Objective: To observe the clinical effect of acupoint sticking of Chinese medicine at Shenque (CV 8) for preventing spleenstomach disharmony caused by venous analgesia pump. Methods: A total of 120 post-surgery pa...Objective: To observe the clinical effect of acupoint sticking of Chinese medicine at Shenque (CV 8) for preventing spleenstomach disharmony caused by venous analgesia pump. Methods: A total of 120 post-surgery patients using venous analgesia pump and also conforming to the inclusion criteria were randomized into 2 groups by the random number table. There were 58 patients in the control group who received conventional post-surgery nursing care, and there were 62 patients in the treatment group who received acupoint sticking at Shenque (CV 8) on the basis of conventional nursing care. After treatment, the incidences of postoperative nausea and vomiting, and abdominal bloating were measured. Results: The total incidence of nausea, vomiting and abdominal bloating in the treatment group was 11.3%, versus 39.7% in the control group, and the difference of the incidence between the two groups showed a statistical significance (P〈0.05). In comparison of the severity of spleen-stomach dishormony which happened during 72 h after surgery, cases in the treatment group showed lower severity than those in the control group (P〈0.05). Conclusion: Acupoint sticking at Shenque (CV 8) can effectively prevent spleen-stomach disharmony caused by venous analgesia pump, which will alleviate sufferings of the patients.展开更多
To treat 30 cases of diarrhea of insufficiency of the spleen-yang type by moxibustion with salt at Shenque (CV 8). The total effective rate was 93.3%. Key Words Diarrhea, chronic - Salt-partitioned Moxibustion - Poin...To treat 30 cases of diarrhea of insufficiency of the spleen-yang type by moxibustion with salt at Shenque (CV 8). The total effective rate was 93.3%. Key Words Diarrhea, chronic - Salt-partitioned Moxibustion - Point CV 8 - Acupuncture-moxibustion Translator: WANG Si-you展开更多
Male S.D rats weighing 200g were used(as experimental animals).Moxibus-tion at Shenque(RN 8)was performed for 15 minutes each day.The course lasted for weeks.In ratsundergoing oral infusion of taurocholate,index of ga...Male S.D rats weighing 200g were used(as experimental animals).Moxibus-tion at Shenque(RN 8)was performed for 15 minutes each day.The course lasted for weeks.In ratsundergoing oral infusion of taurocholate,index of gastric mucosal injury were 9.0±6.1 and 4.6±2.5 in two and four week groups respectively.Compared with control group(index:16.8±7.6),Pwas less than 0.05 and 0.01.This indicated that moxibustion had protective effect on gastric mucosalinjury.Time-effect relationship also existed.In experiment of analysis about mechanism of moxibustion,we found that moxibustion.1.stim-ulated the secretion of gastric mucus(P【0.001);2.increased the PGE 2 content in gastric mucosa(P【0.05);3.increased the number of β-endorphin-like immunoactive cells in gastric mucosa(P【0.05);4.enhanced the transformation of lymphocytes(P【0.01);5.reinforced the function of an-tibody-producing cells in the spleen.The results suggested that protective effect of moxibustion might result from the increase in mu-cus,the PGE 2 and the endogenous β-endorphin.It might also be related to the reinforcing effect ofmoxibustion on transformation of lymphocytes and antibody-producing function of spleen cells.展开更多
Objective: To observe the effect of point sticking therapy on biochemical markers and Rating of Perceived Exertion (RPE) in athletes with exercise-induced fatigue. Methods: A total of 35 football players were rand...Objective: To observe the effect of point sticking therapy on biochemical markers and Rating of Perceived Exertion (RPE) in athletes with exercise-induced fatigue. Methods: A total of 35 football players were randomly allocated into a blank group, a control group and an observation group. The athletes in the blank group received no treatment, those in the control group take Xi Yang Shen (Radix Panacis Quinquefolii) orally and those in the observation group were treated by point sticking therapy. The athletes' RPE, hemoglobin (Hb), red blood cell count (RBC), blood urea nitrogen (BUN) and blood glucose (BG) were recorded every day for analysis. Results: The RPE value in the blank group tends to increase, while that in the treatment and the blank groups tend to increase first but decrease afterwards, and the corresponding RPE value in the observation group was smaller than that in the control group. After treatment, athletes in the blank group showed a decrease in Hb, RBC and BG, but an increase in BUN in the blank group; while athletes in the observation and the control groups showed increases in Hb, RBC and BG, but a decrease in BUN. Conclusion: Point sticking can alleviate clinical symptoms induced by exercise-induced fatigue.展开更多
Objective:To observe the effects of moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)on the energy metabolism and endocrine metabolism indicators of rats undergoing one-time exhaustive swimming,and to expl...Objective:To observe the effects of moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)on the energy metabolism and endocrine metabolism indicators of rats undergoing one-time exhaustive swimming,and to explore the differences between moxibustion at different points in the effects on anti-exercise fatigue.Methods:Forty-eight male SPF rats were randomly divided into a blank group,a model group,a non-meridian and non-acupoint group,a Shenshu(BL 23)group,a Zusanli(ST 36)group,and a Shenque(CV 8)group using random number table method,with eight rats in each group.Except for the blank group,rats in the other groups were subjected to replicating the one-time exhaustive model using the weight-bearing swimming experiment.Except for the model group,the other model rats received mild moxibustion immediately after swimming.Rats in the non-meridian and non-acupoint group received mild moxibustion at bilateral subcostal non-meridian and non-acupoint points,those in the Shenshu(BL 23)group received mild moxibustion at bilateral Shenshu(BL 23),those in the Zusanli(ST 36)group received mild moxibustion at bilateral Zusanli(ST 36),and those in the Shenque(CV 8)group received mild moxibustion at Shenque(CV 8)for 15 min.Four hours after the exhaustive swimming,femoral artery blood was collected to detect blood lactate(BLA),lactate dehydrogenase(LDH),creatine kinase(CK),creatinine(CRE),blood urea nitrogen(BUN),cortisol(C)and testosterone(T)levels,and calculate the T/C ratio.Results:Compared with the blank group,rat's serum levels of BLA,LDH,CK,BUN and C in the model group and the non-meridian and non-acupoint group were increased,and serum levels of CRE and T,and T/C ratios were decreased(P<0.01 or P<0.05);compared with the model group and the non-meridian and non-acupoint group,the serum levels of BLA,LDH,CK,BUN and C in the Shenshu(BL 23)group,Zusanli(ST 36)group and Shenque(CV 8)group were decreased,and the serum CRE and T levels,and the T/C ratios were increased(all P<0.01);compared with the Shenshu(BL 23)group,the serum CK level was decreased in the Shenque(CV 8)group(P<0.01),the serum levels of T and C were decreased in the Zusanli(ST 36)group and Shenque(CV 8)group(P<0.01 or P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.01);compared with the Zusanli(ST 36)group,the serum CK and BUN levels were decreased(P<0.01,P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.05).Conclusion:Moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)shows different anti-fatigue effects by regulating the energy metabolism and endocrine metabolism in rats undergoing one-time exhaustive swimming.Moxibustion at Shenshu(BL 23)is better in promoting energy synthesis.Moxibustion at Shenque(CV 8)is more effective in regulating synthesis and decomposition of the skeletal muscle proteins.展开更多
Objective To explore the effect of moxibustion at Shenque(CV 8)on myocardial structure and function in exercise-induced fatigue rats.Methods A 12-week treadmill running training was performed to create an exercise-ind...Objective To explore the effect of moxibustion at Shenque(CV 8)on myocardial structure and function in exercise-induced fatigue rats.Methods A 12-week treadmill running training was performed to create an exercise-induced fatigue rat model.Sixty eligible male specific-pathogen-free grade Sprague-Dawley rats were randomly divided into a blank group,a control group,a model group,a non-meridian non-acupoint group,a Zusanli(ST 36)group and a Shenque(CV 8)group,with 10 rats in each group.Rats in the blank group did not receive treadmill running training or moxibustion.Rats in the control group did not receive treadmill running training but received mild moxibustion at Shenque(CV 8).Rats in the model group received treadmill running training but no moxibustion.Rats in the non-meridian non-acupoint group,the Zusanli(ST 36)group and the Shenque(CV 8)group received moxibustion at the non-meridian non-acupoint points,Zusanli(ST 36)or Shenque(CV 8)immediately after each treadmill running training,15 min each time,once a day for 5 consecutive days a week at a 2-day interval,60 times of moxibustion in total.Left ventricular end-diastolic diameter(LVEDd),left ventricular end-systolic diameter(LVESd),left ventricular diastolic volume(LVDv),left ventricular systolic volume(LVSv),ejection fraction(EF),stroke volume(SV),early diastolic peak flow velocity of mitral valve(E)and late diastolic peak flow velocity of mitral valve(A)of each group before and after the last treadmill running training were measured.Blood was collected 6 h after the last treadmill running training,and serum C-reactive protein(CRP),myoglobin(Mb),creatine kinase-myocardial band(CK-MB),cardiac troponin I(cTnI)and cardiac troponin T(cTnT)levels were detected.Finally,the heart was separated,the heart mass(HM)was measured,the cTnT level of the myocardial tissue was detected,the ultrastructural changes of the left ventricular myocardium were observed by transmission electron microscope,the left ventricular fraction shortening(LVFS),E/A and heart mass index(HMI)were calculated.Results Compared with the same group before treatment,the rat cardiac LVEDd,LVESd,LVDv,LVSv,SV,E and A were significantly increased(all P<0.01),and the rat LVFS,E/A and EF were significantly decreased(all P<0.01)in the model group and the non-meridian non-acupoint group after treatment;the rat cardiac SV,LVDv,LVSv,E and A were all increased(all P<0.01),while E/A and EF were decreased(all P<0.01)in the Zusanli(ST 36)group after treatment;the rat cardiac LVDv,E and A were significantly increased(P<0.01 or P<0.05),and E/A was significantly decreased(P<0.01)in the Shenque(CV 8)group after treatment.After treatment,compared with the blank group,the rat cardiac LVEDd,LVESd,SV,LVDv,LVSv,E,A,the serum CRP,Mb,CK-MB,cTnI,cTnT and HMI,and the myocardial cTnT were increased(all P<0.01),and the LVFS,E/A and EF were all reduced(all P<0.01)in the model group;compared with the model group and the non-meridian non-acupoint group,rats in the Zusanli(ST 36)group and the Shenque(CV 8)group showed decreased LVEDd,LVESd,SV,LVDv,LVSv,E,A,serum CRP,Mb,CK-MB,cTnI,cTnT and HMI,and myocardial cTnT(P<0.01 or P<0.05),along with increased LVFS,E/A and EF(all P<0.01);compared with the Zusanli(ST 36)group,Mb and A of the Shenque(CV 8)group were decreased(both P<0.01),while both E/A and EF were increased(P<0.01,P<0.05).Transmission electron microscopy examination showed that myofibrils in the blank group and the control group were neatly arranged with clear light and dark bands;the model group and the non-meridian non-acupoint group showed different degrees of myofibril disintegration and breakage,increased and aggregated mitochondria of different sizes,and increased electron density.The myofibrils in the Shenque(CV 8)group and Zusanli(ST 36)group were arranged neatly with clear light and dark bands,and compensatory hyperplasia of mitochondria.Conclusion Moxibustion at Shenque(CV 8)and Zusanli(ST 36)both can effectively improve the occurrence of myocardial remodeling in exercise-induced fatigue rats,and the effect of moxibustion at Shenque(CV 8)is better in improving cardiac function.展开更多
Objective: To observe the effect of Da Huang(Rheum Officinale) application at Shenque(CV 8) on constipation after operation for lumbar vertebral fracture.Methods: Seventy-four patients with constipation after op...Objective: To observe the effect of Da Huang(Rheum Officinale) application at Shenque(CV 8) on constipation after operation for lumbar vertebral fracture.Methods: Seventy-four patients with constipation after operation for lumbar vertebral fracture were divided into an observation group and a control group by the random number table, 37 cases in each group. The two groups both received ordinary treatment(including healthcare guidance, emotional care, diet arrangement, and defecation nursing), while the observation group was additionally given Da Huang(Rheum Officinale) application at Shenque(CV 8) for 6 h each day. The therapeutic efficacy was evaluated after successive 3-day treatment. Results: After 3-day treatment, it took significantly less time for the observation group to conduct the first flatulence and defecation than for the control group(P〈0.05); the therapeutic efficacy of the observation group was significantly higher than that of the control group(P〈0.01). Conclusion: Da Huang(Rheum Officinale) application at Shenque(CV 8) is effective in treating constipation after lumbar vertebral fracture operation, and it's easy-to-operate and well accepted by patients, hence it's proper to promote this method in clinic.展开更多
Objective:To observe the safety and efficacy of moxibustion at Shenque(CV 8)for chronic urticaria.Methods:A total of 80 cases who met the inclusion criteria were randomly allocated into a treatment group and a control...Objective:To observe the safety and efficacy of moxibustion at Shenque(CV 8)for chronic urticaria.Methods:A total of 80 cases who met the inclusion criteria were randomly allocated into a treatment group and a control group,40 in each.Cases in the treatment group were treated with thunder-fire moxibustion at Shenque(CV 8),whereas cases in the control group were treated with Mizolastine.Changes in clinical symptoms such as itching and skin lesion were observed before and after treatment and the therapeutic efficacies were assessed.Results:After treatment,the total scores of clinical symptoms in both groups were markedly reduced(P<0.05),and the reduction was more significant in the treatment group than that in the control group(P<0.05).The total effective rate in the treatment group was 77.5%,versus 65.0%in the control group,showing no significant difference(P>0.05).The Ridit analysis showed a between-group significant difference in therapeutic efficacy grades(P<0.05).There were no reports of adverse reactions in the treatment group and 2 cases experienced mild somnolence in the control group.Conclusion:With an exact effect for chronic urticaria,moxibustion at Shenque(CV 8)obtained better results in overall efficacy and improving symptoms than oral administration of Mizolastine.展开更多
Objective: To investigate the effect of moxibustion at Shenque (CV 8) on free radical metabolism in rat's hippocampus after different degrees of exhaustive exercise. Methods: A total of 72 male Sprague-Dawley (S...Objective: To investigate the effect of moxibustion at Shenque (CV 8) on free radical metabolism in rat's hippocampus after different degrees of exhaustive exercise. Methods: A total of 72 male Sprague-Dawley (SD) rats were randomly divided into a normal group (n=8), a model group (n=32) and a moxibustion group (n=32). According to the times of modeling or treatment, the model group was further randomly divided into different subgroups of a 1-time model subgroup, a 4-time model subgroup, a 7-time model subgroup and a 10-time model subgroup (n=8); the moxibustion group was also further randomly divided into different subgroups of a 1-time moxibustion subgroup, a 4-time moxibustion subgroup, a 7-time moxibustion subgroup and a 10-time moxibustion subgroup (n=8). Rats in both model and moxibustion subgroups were subjected to establishing the swimming exhaustive model. Rats in each moxibustion subgroup received mild moxibustion at Shenque (CV 8) for 15 min immediately after modeling, once every other day. The concentration of malic dialdehyde (MDA), as well as the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in rat's hippocampus in each group were detected 24 h after the exhaustive exercise. Results: Compared with the 1-time model subgroup, the exhaustive swimming time of rats was significantly prolonged in the 4-time model subgroup (P〈0.01), while it was significantly shortened in the 7-time and 10-time model subgroups (both P〈0.01). Compared with the matched model subgroup, the exhaustive swimming time of rats in the 7-time and 10-time moxibustion subgroups was significantly prolonged (both P〈0.01). Compared with the normal group, the MDA concentration was increased significantly (P〈0.01), and the activities stress response of SOD and T-AOC were increased in the 1-time model subgroup (both P〈0.05); the MDA concentration was increased (all P〈0.01), and the activities of SOD, GSH-Px and T-AOC were decreased differently (P〈0.05 or P〈0.01) in the 4-time, 7-time and 10-time model subgroups. Compared with the matched model subgroup, the concentration of MDA was significantly reduced (P〈0.05 or P〈0.01), and the activities of SOD, GSH-Px and T-AOC were significantly increased in the 4-time, 7-time and 10-time moxibustion subgroups (all P〈0.01). Conclusion: Moxibustion at Shenque (CV 8) can improve the fatigue status of the body after long-term exhaustive exercise by regulating free radical metabolism in rat's hippocampus. To some extent, this provides an experimental basis for moxibustion at Shenque (CV 8) against exercise-induced fatigue.展开更多
Objective:To investigate the effect of moxibustion at Shenque(CV 8)on fatigue in rats with chronic exercise-induced exhaustion.Methods:Thirty male Sprague-Dawley(SD)rats were randomly divided into a blank group,a mode...Objective:To investigate the effect of moxibustion at Shenque(CV 8)on fatigue in rats with chronic exercise-induced exhaustion.Methods:Thirty male Sprague-Dawley(SD)rats were randomly divided into a blank group,a model group and a moxibustion group,10 rats in each group.Except rats in the blank group,the remaining rats were subjected to create long-term exhaustion models by repeated swimming.After successful modeling,rats in the moxibustion group received mild moxibustion at Shenque(CV 8)for 15 min,once every other day with a total of 10 times.Rats in the model group and the blank group did not receive moxibustion.At the end of the treatment,the exhausted times,and the body weight of rats before and after the experiment were compared among groups.The levels of blood malondialdehyde(MDA)and urea nitrogen(BUN),as well as the activities of aspartate transarninase(AST),alanine aminotransferase(ALT)and lactate dehydrogenase(LDH)were also measured by the automatic biochemical analyzer,24 h after the exhausting excise.Results:The 10 th swimming time was significantly longer in the moxibustion group than that in the model group(P〈0.01).The increase rate of the body weight was lower in the rats of the moxibustion group than that in the model group before the 7 th and the 10 th exhausting excise(P〈0.05,P〈0.01).The levels of serum MDA and BUN,as well as the activities of AST,ALT and LDH in the model group were higher than those in the blank group(all P〈0.01).The levels of serum MDA and BUN,as well as the activities of AST,ALT and LDH in the moxibustion group were lower than those in the model group(P〈0.01).Conclusion:Moxibustion at Shenque(CV 8)can decrease the serum levels of MDA and BUN,as well as activities of AST,ALT and LDH in the long-term fatigue rats,thus to improve the symptoms of fatigue.展开更多
Objective: To investigate the effect of moxibustion at Shenque (CV8) on the immune system in rats with different levels of exhaustive exercise. Methods: Fifty-six male Sprague-Dawley (SD) rats were randomly divided in...Objective: To investigate the effect of moxibustion at Shenque (CV8) on the immune system in rats with different levels of exhaustive exercise. Methods: Fifty-six male Sprague-Dawley (SD) rats were randomly divided into a blank group (n=8), an exhaustive group (n=24), and a moxibustion group (n=24). The exhaustive group was randomly divided into a 1-time exhaustive group, a 4-time exhaustive group and a 7-time exhaustive group, with 8 rats in each group. According to the treatment time, the moxibustion group was randomly divided into a 1-time moxibustion group, a 4-time moxibustion group and a 7-time moxibustion group, with 8 rats in each group. Rats in the exhaustive groups and the moxibustion groups were subjected to replicating the exhaustive swimming models. Rats in each moxibustion group received mild moxibustion for 15 min immediately after the exhaustive modeling, once every other day. Twenty-four hours after the corresponding exhaustive exercise, the rats in each group were tested for the levels of serum immunoglobulin (Ig) G, IgA, IgM and acid phosphatase (ACP), and the morphological changes of spleen tissues were observed. The level of IgA was detected by immunoturbidimetric assay, and the levels of IgG, IgM and ACP were detected by en zyme-linked imm uno sorb ent assay (ELISA). Results: Compared with the 1-time exhaustive group, swimming time of rats in the 4-time exhaustive group was significantly proIonged (P<0.01), and swimming time of rats in the 7-time exhaustive group was significantly shortened (P<0.01). Compared with the 7-time exhaustive group, exhaustive swimming time of rats in the 7-time moxibustion group was significantly proIonged (P<0.01). Compared with the blank group, the IgG level in the 1-time exhaustive group was significantly decreased (PvO.Ol), and the levels of IgG, IgA and IgM in the 4-time exhaustive group and the 7-time exhaustive group were all sign ifica ntly decreased (P<0.05 or PvO.Ol), while the ACP level was in creased sign ifica ntly (both P<0.01). Microscopically, the number of splenic corpuscles in the 1-time exhaustive group was reduced;the center of some splenic corpuscles in the 4-time exhaustive group was damaged;the number of splenic corpuscles in the 7-time exhaustive group was reduced, and there was no obvious germinal center. Compared with the 4-time exhaustive group, the IgA level in the 4-time moxibusti on group was sign ifica ntly in creased (P<0.01), and the ACP level was sign ifica ntly decreased (P<0.01). Compared with the 7-time exhaustive group, the levels of IgG, IgA and IgM in the 7-time moxibustion group were sign ifica ntly in creased (all PvO.Ol), and the ACP level was sign ifica ntly decreased (P<0.01). Microscopically, the nu mber of splenic corpuscles in the 1-time moxibustion group was reduced;the center of some splenic corpuscles in the 4-time moxibustion group was damaged together with hyperplasia of some splenic corpuscles;blast cells were proliferated in the center of some splenic corpuscles in the 7-time moxibustion group. Conclusion: Moxibustion at Shenque (CV 8) can improve the levels of IgG, IgA and IgM, reduce the ACP level, repair damaged spleen tissues, and enhance the immunity of the body to some extent in the Iong-term fatigue rats.展开更多
文摘Objective:To observe the clinical efficacy of point application with De Huang(Rhizome Rhei Crude)powder at Shenque(CV 8)plus moxa-salt hot compress on the umbilicus for preventing gastrointestinal adverse reactions after chemotherapy for non-Hodgkin lymphoma(NHL).Methods:A total of 60 cases with NHL under chemotherapy were divided into two groups by hospitalization order,with 30 cases in each group.The control group was treated with routine nursing and the observation group was additionally given point application with Da Hueng(Rhizome Rhei Crude)powder plus moxa-salt hot compress on the umbilicus,to compare the effect in preventing gastrointestinal adverse reactions after chemotherapy between the two groups.Results:The occurrence rates of constipation,nausea,vomiting and poor appetite on the second day and fifth day after chemotherapy were obviously lower in the observation group than those in the control group,with statistically significant differences between the two groups(all P〈0.05).Conclusion;The point application with De Hueng(Rhizome Rhei Crude)powder at Shenque(CV 8)plus maxa-salt hot compress on the umbilicus can produce more significant efficacy in preventing the gastrointestinal adverse reactions after chemotherapy for NHL than routine nursing.Moreover,it is simple and easy to be used and popularized.
基金supported by Zhejiang Provincial Hospital of Traditional Chinese Medicine
文摘Objective: To observe the treatment effect of acupoint sticking at Shenque (CV 8) with ginger-preparedBan Xia (Rhizoma Pinelliae) on nausea and vomiting induced by Amifostine for myelodysplastic syndromes (MDS). Methods: Totally 124 eligible subjects intervened by Amifostine were randomized into 2 groups by the visiting order,an observation group and a control group,62 in each group. The control group was intervened by conventional treatment, while the observation group was by acupoint sticking at Shenque (CV 8) with ginger-preparedBan Xia (Rhizoma Pinelliae) in addition to the same conventional treatment. The occurrence rate of nausea and vomiting in the two groups were observed. Results: After intervention, the occurrence rate of nausea and vomiting in the observation group was significantly lower than that in the control group (P&lt;0.01). Conclusion: Acupoint sticking at Shenque (CV 8) with ginger-prepared Ban Xia (Rhizoma Pinelliae)can produce a content effect on nausea and vomiting induced by Amifostine for MDS.
基金the National Natural Science Foundation Projects(No.81671462)Shanxi Natural Science Foundation Projects(No.201901D211315 and 201901D111184)Science and Technology Develop-ment Program of Xinzhou(No.20180103).
文摘Background:Shenque(CV8)acupoint is located on the navel and has been therapeutically used for more than 2000 years in Traditional Chinese Medicine(TCM).However,clinical research on the underlying therapeutic molecular mechanisms of the CV8 acupoint lags far behind.This study aimed to study the mechanisms of umbilical acupoint therapy by using stem cells.Methods:The morphological characteristics of CV8 acupoint were detected under a stereomicroscope using hematoxylin and eosin(H&E)staining.Oil Red,Masson,and immunohistochemical staining on multi-layered slices were used to identify the type of cells at the CV8 acupoint.Cell proliferation was measured by a cell counting kit-8(CCK-8)method.Flow cytometry and immunohistochemistry were used for cell identification.Induced differentiation was used to compare the differentiation of cells derived from CV8 acupoint and non-acupoint somatic stem cells into other cell types,such as osteogenic,adipogenic,and neural stem cell-like cells.Results:Morphological observations showed that adipose tissues at the linea alba of the CV8 acupoint in mice had a mass-like distribution.Immunohistochemical staining confirmed the distribution of stem cell antigen-1(Sca-1)positive cells in the multi-layered slices of CV8 acupoint tissues.Cells isolated from adipose tissues at the CV8 acupoint exhibited high expression of Sca-1 and CD44 and low expression of CD31 and CD34,and these cells possessed osteogenic,adipogenic,and neurogenic stem cell-like cell differentiation ability.The cell proliferation(day 4:0.5138±0.0111 vs.0.4107±0.0180,t=8.447,P=0.0011;day 5:0.6890±0.0070 vs.0.5520±0.0118,t=17.310,P<0.0001;day 6:0.7320±0.0090 vs.0.6157±0.0123,t=13.190,P=0.0002;and day 7:0.7550±0.0050 vs.0.6313±0.0051,t=42.560,P<0.0001),adipogenic([9.224±0.345]%vs.[3.933±1.800]%,t=5.000,P=0.0075),and neurogenic stem cell-like cell differentiation(diameter<50 mm:7.2000±1.3040 vs.2.6000±0.5477,t=7.273,P<0.0001;diameter 50–100 mm:2.6000±0.5477 vs.1.0000±0.7071,t=4.000,P=0.0039;and diameter>100 mm:2.6000±0.5477 vs.0.8000±0.8367,t=4.025,P=0.0038)were significantly enhanced in somatic stem cells derived from the CV8 acupoint compared to somatic stem cells from the groin non-acupoint.However,cells possessed significantly weaker osteogenicity([2.697±0.627]%vs.[7.254±0.958]%,t=6.893,P=0.0023)in the CV8 acupoint group.Conclusions:Our study showed that CV8 acupoint was rich with adipose tissues that contained abundant somatic stem cells.The biological examination of somatic stem cells derived from the CV8 acupoint provided novel insights for future research on the mechanisms of umbilical therapy.
文摘Objective: To observe the clinical effect of acupoint sticking of Chinese medicine at Shenque (CV 8) for preventing spleenstomach disharmony caused by venous analgesia pump. Methods: A total of 120 post-surgery patients using venous analgesia pump and also conforming to the inclusion criteria were randomized into 2 groups by the random number table. There were 58 patients in the control group who received conventional post-surgery nursing care, and there were 62 patients in the treatment group who received acupoint sticking at Shenque (CV 8) on the basis of conventional nursing care. After treatment, the incidences of postoperative nausea and vomiting, and abdominal bloating were measured. Results: The total incidence of nausea, vomiting and abdominal bloating in the treatment group was 11.3%, versus 39.7% in the control group, and the difference of the incidence between the two groups showed a statistical significance (P〈0.05). In comparison of the severity of spleen-stomach dishormony which happened during 72 h after surgery, cases in the treatment group showed lower severity than those in the control group (P〈0.05). Conclusion: Acupoint sticking at Shenque (CV 8) can effectively prevent spleen-stomach disharmony caused by venous analgesia pump, which will alleviate sufferings of the patients.
文摘To treat 30 cases of diarrhea of insufficiency of the spleen-yang type by moxibustion with salt at Shenque (CV 8). The total effective rate was 93.3%. Key Words Diarrhea, chronic - Salt-partitioned Moxibustion - Point CV 8 - Acupuncture-moxibustion Translator: WANG Si-you
文摘Male S.D rats weighing 200g were used(as experimental animals).Moxibus-tion at Shenque(RN 8)was performed for 15 minutes each day.The course lasted for weeks.In ratsundergoing oral infusion of taurocholate,index of gastric mucosal injury were 9.0±6.1 and 4.6±2.5 in two and four week groups respectively.Compared with control group(index:16.8±7.6),Pwas less than 0.05 and 0.01.This indicated that moxibustion had protective effect on gastric mucosalinjury.Time-effect relationship also existed.In experiment of analysis about mechanism of moxibustion,we found that moxibustion.1.stim-ulated the secretion of gastric mucus(P【0.001);2.increased the PGE 2 content in gastric mucosa(P【0.05);3.increased the number of β-endorphin-like immunoactive cells in gastric mucosa(P【0.05);4.enhanced the transformation of lymphocytes(P【0.01);5.reinforced the function of an-tibody-producing cells in the spleen.The results suggested that protective effect of moxibustion might result from the increase in mu-cus,the PGE 2 and the endogenous β-endorphin.It might also be related to the reinforcing effect ofmoxibustion on transformation of lymphocytes and antibody-producing function of spleen cells.
基金supported by Boom Plan Project of Shanghai Municipal Sports Bureau(07TF005)Boom Plan Project of Shanghai Sports Science and Technology(09TF020)+1 种基金Project of Shanghai Science and Technology Committee(10DZ1973800)Shanghai Key Discipline Construction Project(S30304)
文摘Objective: To observe the effect of point sticking therapy on biochemical markers and Rating of Perceived Exertion (RPE) in athletes with exercise-induced fatigue. Methods: A total of 35 football players were randomly allocated into a blank group, a control group and an observation group. The athletes in the blank group received no treatment, those in the control group take Xi Yang Shen (Radix Panacis Quinquefolii) orally and those in the observation group were treated by point sticking therapy. The athletes' RPE, hemoglobin (Hb), red blood cell count (RBC), blood urea nitrogen (BUN) and blood glucose (BG) were recorded every day for analysis. Results: The RPE value in the blank group tends to increase, while that in the treatment and the blank groups tend to increase first but decrease afterwards, and the corresponding RPE value in the observation group was smaller than that in the control group. After treatment, athletes in the blank group showed a decrease in Hb, RBC and BG, but an increase in BUN in the blank group; while athletes in the observation and the control groups showed increases in Hb, RBC and BG, but a decrease in BUN. Conclusion: Point sticking can alleviate clinical symptoms induced by exercise-induced fatigue.
文摘Objective:To observe the effects of moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)on the energy metabolism and endocrine metabolism indicators of rats undergoing one-time exhaustive swimming,and to explore the differences between moxibustion at different points in the effects on anti-exercise fatigue.Methods:Forty-eight male SPF rats were randomly divided into a blank group,a model group,a non-meridian and non-acupoint group,a Shenshu(BL 23)group,a Zusanli(ST 36)group,and a Shenque(CV 8)group using random number table method,with eight rats in each group.Except for the blank group,rats in the other groups were subjected to replicating the one-time exhaustive model using the weight-bearing swimming experiment.Except for the model group,the other model rats received mild moxibustion immediately after swimming.Rats in the non-meridian and non-acupoint group received mild moxibustion at bilateral subcostal non-meridian and non-acupoint points,those in the Shenshu(BL 23)group received mild moxibustion at bilateral Shenshu(BL 23),those in the Zusanli(ST 36)group received mild moxibustion at bilateral Zusanli(ST 36),and those in the Shenque(CV 8)group received mild moxibustion at Shenque(CV 8)for 15 min.Four hours after the exhaustive swimming,femoral artery blood was collected to detect blood lactate(BLA),lactate dehydrogenase(LDH),creatine kinase(CK),creatinine(CRE),blood urea nitrogen(BUN),cortisol(C)and testosterone(T)levels,and calculate the T/C ratio.Results:Compared with the blank group,rat's serum levels of BLA,LDH,CK,BUN and C in the model group and the non-meridian and non-acupoint group were increased,and serum levels of CRE and T,and T/C ratios were decreased(P<0.01 or P<0.05);compared with the model group and the non-meridian and non-acupoint group,the serum levels of BLA,LDH,CK,BUN and C in the Shenshu(BL 23)group,Zusanli(ST 36)group and Shenque(CV 8)group were decreased,and the serum CRE and T levels,and the T/C ratios were increased(all P<0.01);compared with the Shenshu(BL 23)group,the serum CK level was decreased in the Shenque(CV 8)group(P<0.01),the serum levels of T and C were decreased in the Zusanli(ST 36)group and Shenque(CV 8)group(P<0.01 or P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.01);compared with the Zusanli(ST 36)group,the serum CK and BUN levels were decreased(P<0.01,P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.05).Conclusion:Moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)shows different anti-fatigue effects by regulating the energy metabolism and endocrine metabolism in rats undergoing one-time exhaustive swimming.Moxibustion at Shenshu(BL 23)is better in promoting energy synthesis.Moxibustion at Shenque(CV 8)is more effective in regulating synthesis and decomposition of the skeletal muscle proteins.
文摘Objective To explore the effect of moxibustion at Shenque(CV 8)on myocardial structure and function in exercise-induced fatigue rats.Methods A 12-week treadmill running training was performed to create an exercise-induced fatigue rat model.Sixty eligible male specific-pathogen-free grade Sprague-Dawley rats were randomly divided into a blank group,a control group,a model group,a non-meridian non-acupoint group,a Zusanli(ST 36)group and a Shenque(CV 8)group,with 10 rats in each group.Rats in the blank group did not receive treadmill running training or moxibustion.Rats in the control group did not receive treadmill running training but received mild moxibustion at Shenque(CV 8).Rats in the model group received treadmill running training but no moxibustion.Rats in the non-meridian non-acupoint group,the Zusanli(ST 36)group and the Shenque(CV 8)group received moxibustion at the non-meridian non-acupoint points,Zusanli(ST 36)or Shenque(CV 8)immediately after each treadmill running training,15 min each time,once a day for 5 consecutive days a week at a 2-day interval,60 times of moxibustion in total.Left ventricular end-diastolic diameter(LVEDd),left ventricular end-systolic diameter(LVESd),left ventricular diastolic volume(LVDv),left ventricular systolic volume(LVSv),ejection fraction(EF),stroke volume(SV),early diastolic peak flow velocity of mitral valve(E)and late diastolic peak flow velocity of mitral valve(A)of each group before and after the last treadmill running training were measured.Blood was collected 6 h after the last treadmill running training,and serum C-reactive protein(CRP),myoglobin(Mb),creatine kinase-myocardial band(CK-MB),cardiac troponin I(cTnI)and cardiac troponin T(cTnT)levels were detected.Finally,the heart was separated,the heart mass(HM)was measured,the cTnT level of the myocardial tissue was detected,the ultrastructural changes of the left ventricular myocardium were observed by transmission electron microscope,the left ventricular fraction shortening(LVFS),E/A and heart mass index(HMI)were calculated.Results Compared with the same group before treatment,the rat cardiac LVEDd,LVESd,LVDv,LVSv,SV,E and A were significantly increased(all P<0.01),and the rat LVFS,E/A and EF were significantly decreased(all P<0.01)in the model group and the non-meridian non-acupoint group after treatment;the rat cardiac SV,LVDv,LVSv,E and A were all increased(all P<0.01),while E/A and EF were decreased(all P<0.01)in the Zusanli(ST 36)group after treatment;the rat cardiac LVDv,E and A were significantly increased(P<0.01 or P<0.05),and E/A was significantly decreased(P<0.01)in the Shenque(CV 8)group after treatment.After treatment,compared with the blank group,the rat cardiac LVEDd,LVESd,SV,LVDv,LVSv,E,A,the serum CRP,Mb,CK-MB,cTnI,cTnT and HMI,and the myocardial cTnT were increased(all P<0.01),and the LVFS,E/A and EF were all reduced(all P<0.01)in the model group;compared with the model group and the non-meridian non-acupoint group,rats in the Zusanli(ST 36)group and the Shenque(CV 8)group showed decreased LVEDd,LVESd,SV,LVDv,LVSv,E,A,serum CRP,Mb,CK-MB,cTnI,cTnT and HMI,and myocardial cTnT(P<0.01 or P<0.05),along with increased LVFS,E/A and EF(all P<0.01);compared with the Zusanli(ST 36)group,Mb and A of the Shenque(CV 8)group were decreased(both P<0.01),while both E/A and EF were increased(P<0.01,P<0.05).Transmission electron microscopy examination showed that myofibrils in the blank group and the control group were neatly arranged with clear light and dark bands;the model group and the non-meridian non-acupoint group showed different degrees of myofibril disintegration and breakage,increased and aggregated mitochondria of different sizes,and increased electron density.The myofibrils in the Shenque(CV 8)group and Zusanli(ST 36)group were arranged neatly with clear light and dark bands,and compensatory hyperplasia of mitochondria.Conclusion Moxibustion at Shenque(CV 8)and Zusanli(ST 36)both can effectively improve the occurrence of myocardial remodeling in exercise-induced fatigue rats,and the effect of moxibustion at Shenque(CV 8)is better in improving cardiac function.
基金supported by Planning Project of Chinese Medicine Science of Zhejiang ProvinceNo.2012ZB034~~
文摘Objective: To observe the effect of Da Huang(Rheum Officinale) application at Shenque(CV 8) on constipation after operation for lumbar vertebral fracture.Methods: Seventy-four patients with constipation after operation for lumbar vertebral fracture were divided into an observation group and a control group by the random number table, 37 cases in each group. The two groups both received ordinary treatment(including healthcare guidance, emotional care, diet arrangement, and defecation nursing), while the observation group was additionally given Da Huang(Rheum Officinale) application at Shenque(CV 8) for 6 h each day. The therapeutic efficacy was evaluated after successive 3-day treatment. Results: After 3-day treatment, it took significantly less time for the observation group to conduct the first flatulence and defecation than for the control group(P〈0.05); the therapeutic efficacy of the observation group was significantly higher than that of the control group(P〈0.01). Conclusion: Da Huang(Rheum Officinale) application at Shenque(CV 8) is effective in treating constipation after lumbar vertebral fracture operation, and it's easy-to-operate and well accepted by patients, hence it's proper to promote this method in clinic.
基金supported by Jiading Hospital of Traditional Chinese Medicine
文摘Objective:To observe the safety and efficacy of moxibustion at Shenque(CV 8)for chronic urticaria.Methods:A total of 80 cases who met the inclusion criteria were randomly allocated into a treatment group and a control group,40 in each.Cases in the treatment group were treated with thunder-fire moxibustion at Shenque(CV 8),whereas cases in the control group were treated with Mizolastine.Changes in clinical symptoms such as itching and skin lesion were observed before and after treatment and the therapeutic efficacies were assessed.Results:After treatment,the total scores of clinical symptoms in both groups were markedly reduced(P<0.05),and the reduction was more significant in the treatment group than that in the control group(P<0.05).The total effective rate in the treatment group was 77.5%,versus 65.0%in the control group,showing no significant difference(P>0.05).The Ridit analysis showed a between-group significant difference in therapeutic efficacy grades(P<0.05).There were no reports of adverse reactions in the treatment group and 2 cases experienced mild somnolence in the control group.Conclusion:With an exact effect for chronic urticaria,moxibustion at Shenque(CV 8)obtained better results in overall efficacy and improving symptoms than oral administration of Mizolastine.
文摘Objective: To investigate the effect of moxibustion at Shenque (CV 8) on free radical metabolism in rat's hippocampus after different degrees of exhaustive exercise. Methods: A total of 72 male Sprague-Dawley (SD) rats were randomly divided into a normal group (n=8), a model group (n=32) and a moxibustion group (n=32). According to the times of modeling or treatment, the model group was further randomly divided into different subgroups of a 1-time model subgroup, a 4-time model subgroup, a 7-time model subgroup and a 10-time model subgroup (n=8); the moxibustion group was also further randomly divided into different subgroups of a 1-time moxibustion subgroup, a 4-time moxibustion subgroup, a 7-time moxibustion subgroup and a 10-time moxibustion subgroup (n=8). Rats in both model and moxibustion subgroups were subjected to establishing the swimming exhaustive model. Rats in each moxibustion subgroup received mild moxibustion at Shenque (CV 8) for 15 min immediately after modeling, once every other day. The concentration of malic dialdehyde (MDA), as well as the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in rat's hippocampus in each group were detected 24 h after the exhaustive exercise. Results: Compared with the 1-time model subgroup, the exhaustive swimming time of rats was significantly prolonged in the 4-time model subgroup (P〈0.01), while it was significantly shortened in the 7-time and 10-time model subgroups (both P〈0.01). Compared with the matched model subgroup, the exhaustive swimming time of rats in the 7-time and 10-time moxibustion subgroups was significantly prolonged (both P〈0.01). Compared with the normal group, the MDA concentration was increased significantly (P〈0.01), and the activities stress response of SOD and T-AOC were increased in the 1-time model subgroup (both P〈0.05); the MDA concentration was increased (all P〈0.01), and the activities of SOD, GSH-Px and T-AOC were decreased differently (P〈0.05 or P〈0.01) in the 4-time, 7-time and 10-time model subgroups. Compared with the matched model subgroup, the concentration of MDA was significantly reduced (P〈0.05 or P〈0.01), and the activities of SOD, GSH-Px and T-AOC were significantly increased in the 4-time, 7-time and 10-time moxibustion subgroups (all P〈0.01). Conclusion: Moxibustion at Shenque (CV 8) can improve the fatigue status of the body after long-term exhaustive exercise by regulating free radical metabolism in rat's hippocampus. To some extent, this provides an experimental basis for moxibustion at Shenque (CV 8) against exercise-induced fatigue.
文摘Objective:To investigate the effect of moxibustion at Shenque(CV 8)on fatigue in rats with chronic exercise-induced exhaustion.Methods:Thirty male Sprague-Dawley(SD)rats were randomly divided into a blank group,a model group and a moxibustion group,10 rats in each group.Except rats in the blank group,the remaining rats were subjected to create long-term exhaustion models by repeated swimming.After successful modeling,rats in the moxibustion group received mild moxibustion at Shenque(CV 8)for 15 min,once every other day with a total of 10 times.Rats in the model group and the blank group did not receive moxibustion.At the end of the treatment,the exhausted times,and the body weight of rats before and after the experiment were compared among groups.The levels of blood malondialdehyde(MDA)and urea nitrogen(BUN),as well as the activities of aspartate transarninase(AST),alanine aminotransferase(ALT)and lactate dehydrogenase(LDH)were also measured by the automatic biochemical analyzer,24 h after the exhausting excise.Results:The 10 th swimming time was significantly longer in the moxibustion group than that in the model group(P〈0.01).The increase rate of the body weight was lower in the rats of the moxibustion group than that in the model group before the 7 th and the 10 th exhausting excise(P〈0.05,P〈0.01).The levels of serum MDA and BUN,as well as the activities of AST,ALT and LDH in the model group were higher than those in the blank group(all P〈0.01).The levels of serum MDA and BUN,as well as the activities of AST,ALT and LDH in the moxibustion group were lower than those in the model group(P〈0.01).Conclusion:Moxibustion at Shenque(CV 8)can decrease the serum levels of MDA and BUN,as well as activities of AST,ALT and LDH in the long-term fatigue rats,thus to improve the symptoms of fatigue.
文摘Objective: To investigate the effect of moxibustion at Shenque (CV8) on the immune system in rats with different levels of exhaustive exercise. Methods: Fifty-six male Sprague-Dawley (SD) rats were randomly divided into a blank group (n=8), an exhaustive group (n=24), and a moxibustion group (n=24). The exhaustive group was randomly divided into a 1-time exhaustive group, a 4-time exhaustive group and a 7-time exhaustive group, with 8 rats in each group. According to the treatment time, the moxibustion group was randomly divided into a 1-time moxibustion group, a 4-time moxibustion group and a 7-time moxibustion group, with 8 rats in each group. Rats in the exhaustive groups and the moxibustion groups were subjected to replicating the exhaustive swimming models. Rats in each moxibustion group received mild moxibustion for 15 min immediately after the exhaustive modeling, once every other day. Twenty-four hours after the corresponding exhaustive exercise, the rats in each group were tested for the levels of serum immunoglobulin (Ig) G, IgA, IgM and acid phosphatase (ACP), and the morphological changes of spleen tissues were observed. The level of IgA was detected by immunoturbidimetric assay, and the levels of IgG, IgM and ACP were detected by en zyme-linked imm uno sorb ent assay (ELISA). Results: Compared with the 1-time exhaustive group, swimming time of rats in the 4-time exhaustive group was significantly proIonged (P<0.01), and swimming time of rats in the 7-time exhaustive group was significantly shortened (P<0.01). Compared with the 7-time exhaustive group, exhaustive swimming time of rats in the 7-time moxibustion group was significantly proIonged (P<0.01). Compared with the blank group, the IgG level in the 1-time exhaustive group was significantly decreased (PvO.Ol), and the levels of IgG, IgA and IgM in the 4-time exhaustive group and the 7-time exhaustive group were all sign ifica ntly decreased (P<0.05 or PvO.Ol), while the ACP level was in creased sign ifica ntly (both P<0.01). Microscopically, the number of splenic corpuscles in the 1-time exhaustive group was reduced;the center of some splenic corpuscles in the 4-time exhaustive group was damaged;the number of splenic corpuscles in the 7-time exhaustive group was reduced, and there was no obvious germinal center. Compared with the 4-time exhaustive group, the IgA level in the 4-time moxibusti on group was sign ifica ntly in creased (P<0.01), and the ACP level was sign ifica ntly decreased (P<0.01). Compared with the 7-time exhaustive group, the levels of IgG, IgA and IgM in the 7-time moxibustion group were sign ifica ntly in creased (all PvO.Ol), and the ACP level was sign ifica ntly decreased (P<0.01). Microscopically, the nu mber of splenic corpuscles in the 1-time moxibustion group was reduced;the center of some splenic corpuscles in the 4-time moxibustion group was damaged together with hyperplasia of some splenic corpuscles;blast cells were proliferated in the center of some splenic corpuscles in the 7-time moxibustion group. Conclusion: Moxibustion at Shenque (CV 8) can improve the levels of IgG, IgA and IgM, reduce the ACP level, repair damaged spleen tissues, and enhance the immunity of the body to some extent in the Iong-term fatigue rats.