OBJECTIVE: To investigate the effect of acupuncture at acupoint of Shenshu (BL 23) in ovariectomized rats and sham-operated rats. METHODS: Female Sprague-Dawley rats were divided into the following four groups (n...OBJECTIVE: To investigate the effect of acupuncture at acupoint of Shenshu (BL 23) in ovariectomized rats and sham-operated rats. METHODS: Female Sprague-Dawley rats were divided into the following four groups (n = 12 each): sham operation only (Sham), sham operation with acupuncture (Sham + ACP), ovariectomy only (OVX), and ovariectomy with acupuncture (OVX + ACP). Operations were performed at the 9th week of age. Acupuncture of Shenshu (BL 23) was started at the 1 lth week of age and conducted 3 times per week until the 26th week of age. RESULTS: Among ovariectomized rats, the acupuncture-treated rats had significantly lower body weights and cross-linked N-telopeptide of type 1 collagen levels in the urine after 3 weeks of acu- puncture treatment, higher bone mineral density of the lumbar vertebra after 7 weeks, and smaller trabecular separation of the sixth lumbar vertebra, measured by micro-computed tomography, after 17 weeks than control rats. These effects were not observed in sham-operated rats. Blood concentrations of estradiol were significantly higher in the OVX + ACP group than in the untreated group. Acupuncture treatment increased plasma testosterone levels in sham-operated rats, while this increase was not observed in OVX rats. CONCLUSION: Acupuncture of Shenshu (BL 23) prevents bone loss and structural changes by sup- pressing bone resorption.展开更多
Lumbar three point needling”, meaning puncturing Shenshu (BL 23), Dachangshu (BL 25) and Ciliao(BL 32) at the same time, is a commonly used acupuncture method in the treatment of lumbocrural diseases. Points suppleme...Lumbar three point needling”, meaning puncturing Shenshu (BL 23), Dachangshu (BL 25) and Ciliao(BL 32) at the same time, is a commonly used acupuncture method in the treatment of lumbocrural diseases. Points supplemented according to symptoms: Weizhong(BL 40) is supplemented for lumbar vertebra hypertrophy spondylitis; Weizhong (BL 40) and Kunlun(BL 60) supplemented for acute lumbar muscle sprain; Zhibian (BL 54), Huantiao (GB 30) and Ahshi points supplemented for nervi cluniam superior inflammation, and Sanyinjiao (SP 6) and Mingmen(GV 4) supplemented for impotence. Gauge 28 2 cun long needles are used. The needles are inserted perpendicularly into Shenshu(BL 23), Dachengshu(BL 25) and Ciliao(BL 32) to a depth of about 1.2~1.5 cun. Other supplementary points are needled and stimulated with routine manipulations, generally the reducing method is used for excess syndrome, and the reinforcing method applied to deficiency syndrome. The author, on the basis of many years of clinical practice, considers that “lumbar three point needling” is fairly effective in treating lumbosacral diseases.展开更多
Objective: To investigate the effects of acupuncture of Shenshu (BL 23), Huiyang (BL 35) and Shenshu (BL 23) plus Huiyang (BL 35) on the main symptoms of unstable bladder (USB) and explore the specificity o...Objective: To investigate the effects of acupuncture of Shenshu (BL 23), Huiyang (BL 35) and Shenshu (BL 23) plus Huiyang (BL 35) on the main symptoms of unstable bladder (USB) and explore the specificity of different points in treating USB through a clinical randomized controlled trial. Methods: Eighty-nine USB patients were allocated to three groups: Shenshu (BL 23), Huiyang (BL 35) and Shenshu (BL 23) plus Huiyang (BL 35). I-PSS was used as an observation index. The effects were evaluated and a comparison was made between the groups after three and six treatments. Results: I-PSS significantly improved in all Shenshu (BL 23), Huiyang (BL 35) and Shenshu (BL 23) plus Huiyang (BL 35) groups of USB patients (P〈0.05). The effect was significantly better in the Huiyang (BL 35) and Shenshu (BL 23) plus Huiyang (BL 35) groups than in the Shenshu (BL 23) group (P〈0.05). The efficacy rate was 50.0% in the Shenshu (BL 23) group, 82.8% in the Huiyang (BL 35) group and 90.0% in the Shenshu (BL 23) plus Huiyang (BL 35) group. It was significantly higher in the Huiyang (BL 35) and Shenshu (BL 23) plus Huiyang (BL 35) groups than in the Shenshu (BL 23) group (P〈0.05). Conclusion: Points Shenshu (BL 23), Huiyang (BL 35) and Shenshu (BL 23) plus Huiyang (BL 35) can all regulate voiding dysfunction and improve quality of life in USB patients. Shenshu (BL 23) plus Huiyang (BL 35) has the most marked therapeutic effect.展开更多
Objective: To discuss the acupoints selection pattern in acupuncture-moxibustion treatment of perimenopausal syndrome(PMS) from 2007 to 2016. Methods: Clinical literatures related to PMS treated with acupuncture-m...Objective: To discuss the acupoints selection pattern in acupuncture-moxibustion treatment of perimenopausal syndrome(PMS) from 2007 to 2016. Methods: Clinical literatures related to PMS treated with acupuncture-moxibustion published from 2007 to 2016 were collected from Chinese Biomedical Literature Database(CBM), Chongqing VIP Database(CQVIP), China National Knowledge Infrastructure(CNKI), and Wanfang Academic Journal Full-text Database(Wanfang). The retrieved data underwent descriptive analysis, cluster analysis and association pattern analysis to determine the acupoints selection principle in acupuncture-moxibustion treatment of PMS. Results: The top five acupoints used in acupuncture-moxibustion treatment of PMS were Sanyinjiao(SP 6), Shenshu(BL 23), Guanyuan(CV 4), Baihui(GV 20), and Shenmen(HT 7). The leading 4 meridians were Bladder Meridian, Conception Vessel, Spleen Meridian, and Governor Vessel. The clustering analysis showed that the 5 core acupoint groups were:(1) Sanyinjiao(SP 6);(2) Shenshu(BL 23) and Guanyuan(CV 4);(3) Baihui(GV 20), Shenmen(HT 7), Zusanli(ST 36), Ganshu(BL 18) and Taichong(LR 3);(4) Taixi(KI 3), Pishu(BL 20), Xinshu(BL 15), Qihai(CV 6) and Neiguan(PC 6);(5) Sishencong(EX-HN 1), Zhongwan(CV 12), Hegu(LI 4), Yintang(GV 29), Fengchi(GB 20), Zhongji(CV 3) and Feishu(BL 13). The three most significant acupoints were Sanyinjiao(SP 6), Shenshu(BL 23) and Guanyuan(CV 4). Acupoint groups based on syndrome differentiation included:(1) Hegu(LI 4), Zhongwan(CV 12) and Sishencong(EX-HN 1);(2) Feishu(BL 13), Zhongji(CV 3), Fengchi(GB 20) and Yintang(GV 29);(3) Xinshu(BL 15), Pishu(BL 20), Qihai(CV 6), Neiguan(PC 6) and Taixi(KI 3);(4) Ganshu(BL 18), Zusanli(ST 36), Shenmen(HT 7), Taichong(LR 3) and Baihui(GV 20). The analysis of association pattern elaborated that Shenshu(BL 23) and Sanyinjiao(SP 6) won the highest support rate in the paired groups; Ganshu(BL 18), Shenshu(BL 23) and Sanyinjiao(SP 6) had the highest support rate among the acupoint groups. Conclusion: The data mining results of acupuncture-moxibustion treatment of PMS substantially conform to the general principle in traditional acupuncture-moxibustion theories, able to reflect the acupoints selection and grouping pattern and provide references for acupuncture-moxibustion treatment of PMS.展开更多
Objective:To observe the clinical efficacy of acupoint injection with salmon calcitonin for back pain in elderly patients with primary osteoporosis.Methods:A total of 76 patients were selected and randomly divided int...Objective:To observe the clinical efficacy of acupoint injection with salmon calcitonin for back pain in elderly patients with primary osteoporosis.Methods:A total of 76 patients were selected and randomly divided into two groups by the random number table method,with 39 cases in the treatment group and 37 cases in the control group,respectively.Patients in both groups received routine anti-osteoporosis treatment.Patients in the treatment group received additional acupoint injection with salmon calcitonin at bilateral Pishu(BL 20)and Shenshu(BL 23),while patients in the control group received additional intramuscular injection with salmon calcitonin.The treatments for both groups were given once a day and lasted for 4 weeks.Visual analog scale(VAS)and Chinese Oswestry disability index(CODI)scores were observed before treatment,after 2 weeks and 4 weeks of treatment,and the use of analgesics during the treatment were recorded.Results:After treatment,the clinical efficacy in the treatment group was more significant than that in the control group(P<0.05).After 2 weeks and 4 weeks of treatment,the VAS scores in both groups showed significant intra-group and between-group differences(all P<0.05),and the CODI scores in both groups showed significant intra-group differences(all P<0.05).After 2 weeks of treatment,the CODI score showed no significant between-group difference(P>0.05).After 4 weeks of treatment,the improvement of CODI score in the treatment group was more significant than that in the control group(P<0.05).During the treatment,2 cases in the treatment group took analgesics versus 8 cases in the control group,and the result showed a significant between-group difference(P<0.05).Conclusion:For back pain in elderly patients with primary osteoporosis,based on the routine treatment of oral medication,the clinical efficacy of acupoint injection with salmon calcitonin at bilateral Pishu(BL 20)and Shenshu(BL 23)is more significant than that of intramuscular injection.Acupoint injection treatment can improve patients'conditions and reduce the use of analgesics.展开更多
Objective:To observe the effects of moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)on the energy metabolism and endocrine metabolism indicators of rats undergoing one-time exhaustive swimming,and to expl...Objective:To observe the effects of moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)on the energy metabolism and endocrine metabolism indicators of rats undergoing one-time exhaustive swimming,and to explore the differences between moxibustion at different points in the effects on anti-exercise fatigue.Methods:Forty-eight male SPF rats were randomly divided into a blank group,a model group,a non-meridian and non-acupoint group,a Shenshu(BL 23)group,a Zusanli(ST 36)group,and a Shenque(CV 8)group using random number table method,with eight rats in each group.Except for the blank group,rats in the other groups were subjected to replicating the one-time exhaustive model using the weight-bearing swimming experiment.Except for the model group,the other model rats received mild moxibustion immediately after swimming.Rats in the non-meridian and non-acupoint group received mild moxibustion at bilateral subcostal non-meridian and non-acupoint points,those in the Shenshu(BL 23)group received mild moxibustion at bilateral Shenshu(BL 23),those in the Zusanli(ST 36)group received mild moxibustion at bilateral Zusanli(ST 36),and those in the Shenque(CV 8)group received mild moxibustion at Shenque(CV 8)for 15 min.Four hours after the exhaustive swimming,femoral artery blood was collected to detect blood lactate(BLA),lactate dehydrogenase(LDH),creatine kinase(CK),creatinine(CRE),blood urea nitrogen(BUN),cortisol(C)and testosterone(T)levels,and calculate the T/C ratio.Results:Compared with the blank group,rat's serum levels of BLA,LDH,CK,BUN and C in the model group and the non-meridian and non-acupoint group were increased,and serum levels of CRE and T,and T/C ratios were decreased(P<0.01 or P<0.05);compared with the model group and the non-meridian and non-acupoint group,the serum levels of BLA,LDH,CK,BUN and C in the Shenshu(BL 23)group,Zusanli(ST 36)group and Shenque(CV 8)group were decreased,and the serum CRE and T levels,and the T/C ratios were increased(all P<0.01);compared with the Shenshu(BL 23)group,the serum CK level was decreased in the Shenque(CV 8)group(P<0.01),the serum levels of T and C were decreased in the Zusanli(ST 36)group and Shenque(CV 8)group(P<0.01 or P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.01);compared with the Zusanli(ST 36)group,the serum CK and BUN levels were decreased(P<0.01,P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.05).Conclusion:Moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)shows different anti-fatigue effects by regulating the energy metabolism and endocrine metabolism in rats undergoing one-time exhaustive swimming.Moxibustion at Shenshu(BL 23)is better in promoting energy synthesis.Moxibustion at Shenque(CV 8)is more effective in regulating synthesis and decomposition of the skeletal muscle proteins.展开更多
Objective: To observe the effect of electroacupuncture (EA) on the expression of erythropoie-tin-producing hepatocyte receptor B2 (EphB2) in the cortex around the infracted area of middle cerebral artery occlusi...Objective: To observe the effect of electroacupuncture (EA) on the expression of erythropoie-tin-producing hepatocyte receptor B2 (EphB2) in the cortex around the infracted area of middle cerebral artery occlusion (MCAO) rats at different timing, and to reveal the possible mechanism of acupuncture in the treatment of cerebral ischemia. Methods: A total of 180 male Sprague-Dawley (SD) rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, with 45 rats in each group. Rats in each group were further divided into three subgroups: postoperative 3 d, postoperative 14 d and postoperative 2l d groups, with 15 rats in each subgroup. The MCAO model was made by the modified occlusion method. The neurological function score, 2,3,5-triphenyl tetrazolium chloride (TFC) staining, immunohistochemistry assay, immunofluorescence double labeling method and Western blot were used to detect the corresponding indicators. Results: The neurological impairment of rats was most obvious at postoperative 3 d, and then gradually improved with time, which was more significant in the acupoint group (P〈0.05). The change of infarcted volume was consistent with the neurological function impairment. The number of EphB2 positive cells (EphB2+) around the infarcted area was decreased significantly at postoperative 3 d, and then gradually improved with time, which returned to the same level as that in the sham operation group at postoperative 21 d. The increase was most significant in the acupoint group (P〈0.05), and the positive cell number was higher than that in the sham operation group (P〈0.01). Western blot and immunohistochemistry results were basically consistent. Immunofluorescence displayed that EphB2+ and postsynaptic density-95 positive (PSD-95+) were co-expressed, after the MCAO operation, in the cortical neuron around the infracted area, and the number of co-expressing cells was increased gradually with time, which was most significant in the acupoint group (P〈0.05). Conclusion: Electroacupuncture at Ganshu (BL 18) and Shenshu (BL 23) can significantly improve the neurological function and cerebral infarcted volume ratio of MCAO rats, which may be related to the activation of EphB2 expression in cortex around the infracted area and the promotion of synaptic remodeling.展开更多
【目的】观察电针百会、风府、双侧肾俞穴联合人脐带间充质干细胞(hUC-MSCs)移植对缺血性脑损伤大鼠的脑保护作用及机制。【方法】将40只SD大鼠随机分为正常组、模型组、移植组、联合组,每组10只。除正常组,其他各组大鼠建立脑缺血再灌...【目的】观察电针百会、风府、双侧肾俞穴联合人脐带间充质干细胞(hUC-MSCs)移植对缺血性脑损伤大鼠的脑保护作用及机制。【方法】将40只SD大鼠随机分为正常组、模型组、移植组、联合组,每组10只。除正常组,其他各组大鼠建立脑缺血再灌注损伤模型。在造模结束24 h后,移植组大鼠给予0.5 m L 2×10^(6)个hUC-MSCs细胞悬液一次性尾静脉植入,联合组在移植组治疗基础上,给予电针百会穴、风府穴、双侧肾俞穴,每次30 min,每日1次,连续针刺3周。治疗结束后,苏木素-伊红(HE)染色法观察海马组织病理形态,脱氧核糖核苷酸末端转移酶介导的缺口末端标记(TUNEL)法观察脑组织细胞凋亡情况,免疫荧光法检测脑组织腺苷A2A受体(ADORA2A)表达,免疫组织化学法检测脑组织糖原合酶激酶3β(GSK-3β)、β-连环蛋白(β-catenin)表达,Western Blot法检测脑组织跨膜蛋白闭锁蛋白(Occludin)、胞质附着蛋白(ZO-1)表达。【结果】与正常组比较,模型组脑组织细胞凋亡率升高,脑组织ADORA2A阳性表达率及GSK-3β蛋白表达水平升高,Occludin、ZO-1、β-catenin蛋白表达水平降低(P<0.05),HE染色结果显示,模型组海马组织结构明显异常;与模型组比较,移植组和联合组大鼠脑组织细胞凋亡率降低,脑组织ADORA2A阳性表达率及GSK-3β蛋白表达水平降低,Occludin、ZO-1、β-catenin蛋白表达水平升高(P<0.05),海马组织病理程度明显减轻;与移植组比较,联合组脑组织细胞凋亡率降低,脑组织ADORA2A阳性表达率及GSK-3β蛋白表达水平降低,Occludin、ZO-1、β-catenin蛋白表达水平升高(P<0.05)。【结论】电针百会、风府、双侧肾俞穴联合hUC-MSCs可改善缺血性脑损伤大鼠血脑屏障,抑制脑组织ADORA2A、GSK-3β表达,提高β-catenin表达,抑制脑组织细胞凋亡,起到脑保护作用,且作用效果优于单纯hUC-MSCs移植。展开更多
Objective:To observe the effect of moxibustion on learning and memory abilities,corticosterone and glucocorticoid receptor(GR) in subacute aging rats.Methods:Twenty four Sprague-Dawley(SD) rats were randomly div...Objective:To observe the effect of moxibustion on learning and memory abilities,corticosterone and glucocorticoid receptor(GR) in subacute aging rats.Methods:Twenty four Sprague-Dawley(SD) rats were randomly divided into a normal group,a model group and a moxibustion group,8 rats in each group.Rats in the model group and the moxibustion group were subcutaneously injected with 25% D-galactose [125 mg/(kg·bw)] for 40 d continuous;rats in the normal group were injected with saline at the same position for 40 d continuous.Rats in the moxibustion group were given mild moxibustion at bilateral Shenshu(BL 23) at the same time of modeling;rats in the normal group and the model group were only identically grabbed without moxibustion for 40 d.The learning and memory abilities of rats were observed using the Morris water maze at the end of the experiment.Abdominal aorta blood and thymus were collected after water maze experiment.Enzyme-linked immunosorbent assay(ELISA) was used to detect serum corticosterone level,and immunohistochemical method was used to detect the expression of thymus GR.Results:Compared with the normal group,rats in the model group showed that a significantly longer escape latency time(P〈0.01) on the third and the fourth days;number of times crossing the platform in 70 s significantly reduced(P〈0.01);activity times in the fourth quadrant significantly decreased(P〈0.05);serum corticosterone levels increased(P〈0.01);thymus GR expression decreased(P〈0.05).Compared with the model group,rats in the moxibustion group showed that the escape latency times were significantly shorter on the third,the fourth and the fifth days(P〈0.01,P〈0.05);number of times crossing the platform in 70 s significantly increased(P〈0.05);activity times in the fourth quadrant significantly increased(P〈0.05);serum corticosterone levels decreased(P〈0.05);thymus GR expression increased(P〈0.05).Conclusion:Moxibustion could improve the learning and memory abilities of subacute aging rats,down-regulate serum corticosterone levels,and increase thymus GR content.展开更多
OBJECTIVE:To examine whether specific stimulation of Shenshu(BL23)affects sympathetic nervous activity(SNA)-associated plasma renin concentration(PRC).METHODS:Eight healthy volunteers participated in three pattern con...OBJECTIVE:To examine whether specific stimulation of Shenshu(BL23)affects sympathetic nervous activity(SNA)-associated plasma renin concentration(PRC).METHODS:Eight healthy volunteers participated in three pattern conditions in random order:control(Cont),stimulation of Shenshu(BL23),and stimulation of sham point(Sham).All participants were initially in the supine position for>60 min,and then remained in the standing position during the experimental procedure to increase SNA.An electrocardiogram was used to calculate low frequency/high frequency(LF/HF)ratio;blood was collected to analyze PRC.RESULTS:The LF/HF ratio was significantly increased in the standing position when compared with the supine position(P<0.01).There was no difference in LF/HF ratio during or after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,the LF/HF ratio was significantly increased in Cont and Sham conditions(P<0.01).There was no difference in PRC after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,there was a significant increase in PRC in the Cont and Sham conditions(Cont P<0.05,Sham P<0.01).CONCLUSION:Our results demonstrated that specific acupuncture stimulation of Shenshu(BL23)in the standing position decreased SNA-associated PRC,which was not observed during acupuncture stimulation of the sham point.展开更多
OBJECTIVE: It is unknown whether the psoas major muscle, thought to be a key muscle for treatment of lower back pain, can be punctured at Shenshu(BL23).METHODS: Twelve dissected specimens were used for studying th...OBJECTIVE: It is unknown whether the psoas major muscle, thought to be a key muscle for treatment of lower back pain, can be punctured at Shenshu(BL23).METHODS: Twelve dissected specimens were used for studying the needling pathway of BL23 by perpendicularly inserting the depth-measuring blade of a vernier caliper at BL23. Dimensions of psoas muscle were measured. Correlation studies were conducted. In addition, our samples were grouped by gender and underlying medical conditions for analysis.RESULTS: Half(50%) of the needle insertions successfully punctured psoas muscle. The mean depth of needle insertion to puncture psoas muscle(D_(min)) in the group with short-term underlying medical conditions was 38.0 mm(interquartile range 29.0–51.8 mm), approximately 6 mm deeper than 32.0(29.3–42.5) mm in the group with long-term health problems(P = 0.041). The cross-sectional area(CSA) of psoas muscle in the former group was on average approximately 1.5 times that of the latter group(P = 0.04). When the data were analysed by gender, the thickness of psoas muscle in the male group was 19.0(6.5–24.0) mm compared to 19.5(5.8–34.8) mm in the female group(P = 0.02). The age in the female group(P = 0.04) and the body length of the total group(P = 0.04) negatively correlated to D_(min).CONCLUSION: Needle insertion at BL23 might be able to puncture psoas muscle. Differences in the CSA of psoas muscle and D_(min) were observed in groups with short-term and long-term underlying medical conditions.展开更多
基金Supported by"High-Tech Research Center"Project for Private Universities:Matching Fund Subsidy from Ministry of Education,Culture,Sports,Science,and Technology(No.2006-2010)
文摘OBJECTIVE: To investigate the effect of acupuncture at acupoint of Shenshu (BL 23) in ovariectomized rats and sham-operated rats. METHODS: Female Sprague-Dawley rats were divided into the following four groups (n = 12 each): sham operation only (Sham), sham operation with acupuncture (Sham + ACP), ovariectomy only (OVX), and ovariectomy with acupuncture (OVX + ACP). Operations were performed at the 9th week of age. Acupuncture of Shenshu (BL 23) was started at the 1 lth week of age and conducted 3 times per week until the 26th week of age. RESULTS: Among ovariectomized rats, the acupuncture-treated rats had significantly lower body weights and cross-linked N-telopeptide of type 1 collagen levels in the urine after 3 weeks of acu- puncture treatment, higher bone mineral density of the lumbar vertebra after 7 weeks, and smaller trabecular separation of the sixth lumbar vertebra, measured by micro-computed tomography, after 17 weeks than control rats. These effects were not observed in sham-operated rats. Blood concentrations of estradiol were significantly higher in the OVX + ACP group than in the untreated group. Acupuncture treatment increased plasma testosterone levels in sham-operated rats, while this increase was not observed in OVX rats. CONCLUSION: Acupuncture of Shenshu (BL 23) prevents bone loss and structural changes by sup- pressing bone resorption.
文摘Lumbar three point needling”, meaning puncturing Shenshu (BL 23), Dachangshu (BL 25) and Ciliao(BL 32) at the same time, is a commonly used acupuncture method in the treatment of lumbocrural diseases. Points supplemented according to symptoms: Weizhong(BL 40) is supplemented for lumbar vertebra hypertrophy spondylitis; Weizhong (BL 40) and Kunlun(BL 60) supplemented for acute lumbar muscle sprain; Zhibian (BL 54), Huantiao (GB 30) and Ahshi points supplemented for nervi cluniam superior inflammation, and Sanyinjiao (SP 6) and Mingmen(GV 4) supplemented for impotence. Gauge 28 2 cun long needles are used. The needles are inserted perpendicularly into Shenshu(BL 23), Dachengshu(BL 25) and Ciliao(BL 32) to a depth of about 1.2~1.5 cun. Other supplementary points are needled and stimulated with routine manipulations, generally the reducing method is used for excess syndrome, and the reinforcing method applied to deficiency syndrome. The author, on the basis of many years of clinical practice, considers that “lumbar three point needling” is fairly effective in treating lumbosacral diseases.
基金National Nature Science Fund(30572410)Shanghai Leading Research Discipline Project(T0302)
文摘Objective: To investigate the effects of acupuncture of Shenshu (BL 23), Huiyang (BL 35) and Shenshu (BL 23) plus Huiyang (BL 35) on the main symptoms of unstable bladder (USB) and explore the specificity of different points in treating USB through a clinical randomized controlled trial. Methods: Eighty-nine USB patients were allocated to three groups: Shenshu (BL 23), Huiyang (BL 35) and Shenshu (BL 23) plus Huiyang (BL 35). I-PSS was used as an observation index. The effects were evaluated and a comparison was made between the groups after three and six treatments. Results: I-PSS significantly improved in all Shenshu (BL 23), Huiyang (BL 35) and Shenshu (BL 23) plus Huiyang (BL 35) groups of USB patients (P〈0.05). The effect was significantly better in the Huiyang (BL 35) and Shenshu (BL 23) plus Huiyang (BL 35) groups than in the Shenshu (BL 23) group (P〈0.05). The efficacy rate was 50.0% in the Shenshu (BL 23) group, 82.8% in the Huiyang (BL 35) group and 90.0% in the Shenshu (BL 23) plus Huiyang (BL 35) group. It was significantly higher in the Huiyang (BL 35) and Shenshu (BL 23) plus Huiyang (BL 35) groups than in the Shenshu (BL 23) group (P〈0.05). Conclusion: Points Shenshu (BL 23), Huiyang (BL 35) and Shenshu (BL 23) plus Huiyang (BL 35) can all regulate voiding dysfunction and improve quality of life in USB patients. Shenshu (BL 23) plus Huiyang (BL 35) has the most marked therapeutic effect.
基金supported by Scientific Research Project of Hebei Provincial Administration Bureau of Traditional Chinese Medicine,No.2015162Hebei Tangshan Science and Technology Project,No.14130264B+1 种基金Cultivation Fund of North China University of Science and Technology,No.GP201512Undergraduate Innovation and Entrepreneurship Training Programs of North China University of Science and Technology,No.X2015203,No.X2016281~~
文摘Objective: To discuss the acupoints selection pattern in acupuncture-moxibustion treatment of perimenopausal syndrome(PMS) from 2007 to 2016. Methods: Clinical literatures related to PMS treated with acupuncture-moxibustion published from 2007 to 2016 were collected from Chinese Biomedical Literature Database(CBM), Chongqing VIP Database(CQVIP), China National Knowledge Infrastructure(CNKI), and Wanfang Academic Journal Full-text Database(Wanfang). The retrieved data underwent descriptive analysis, cluster analysis and association pattern analysis to determine the acupoints selection principle in acupuncture-moxibustion treatment of PMS. Results: The top five acupoints used in acupuncture-moxibustion treatment of PMS were Sanyinjiao(SP 6), Shenshu(BL 23), Guanyuan(CV 4), Baihui(GV 20), and Shenmen(HT 7). The leading 4 meridians were Bladder Meridian, Conception Vessel, Spleen Meridian, and Governor Vessel. The clustering analysis showed that the 5 core acupoint groups were:(1) Sanyinjiao(SP 6);(2) Shenshu(BL 23) and Guanyuan(CV 4);(3) Baihui(GV 20), Shenmen(HT 7), Zusanli(ST 36), Ganshu(BL 18) and Taichong(LR 3);(4) Taixi(KI 3), Pishu(BL 20), Xinshu(BL 15), Qihai(CV 6) and Neiguan(PC 6);(5) Sishencong(EX-HN 1), Zhongwan(CV 12), Hegu(LI 4), Yintang(GV 29), Fengchi(GB 20), Zhongji(CV 3) and Feishu(BL 13). The three most significant acupoints were Sanyinjiao(SP 6), Shenshu(BL 23) and Guanyuan(CV 4). Acupoint groups based on syndrome differentiation included:(1) Hegu(LI 4), Zhongwan(CV 12) and Sishencong(EX-HN 1);(2) Feishu(BL 13), Zhongji(CV 3), Fengchi(GB 20) and Yintang(GV 29);(3) Xinshu(BL 15), Pishu(BL 20), Qihai(CV 6), Neiguan(PC 6) and Taixi(KI 3);(4) Ganshu(BL 18), Zusanli(ST 36), Shenmen(HT 7), Taichong(LR 3) and Baihui(GV 20). The analysis of association pattern elaborated that Shenshu(BL 23) and Sanyinjiao(SP 6) won the highest support rate in the paired groups; Ganshu(BL 18), Shenshu(BL 23) and Sanyinjiao(SP 6) had the highest support rate among the acupoint groups. Conclusion: The data mining results of acupuncture-moxibustion treatment of PMS substantially conform to the general principle in traditional acupuncture-moxibustion theories, able to reflect the acupoints selection and grouping pattern and provide references for acupuncture-moxibustion treatment of PMS.
文摘Objective:To observe the clinical efficacy of acupoint injection with salmon calcitonin for back pain in elderly patients with primary osteoporosis.Methods:A total of 76 patients were selected and randomly divided into two groups by the random number table method,with 39 cases in the treatment group and 37 cases in the control group,respectively.Patients in both groups received routine anti-osteoporosis treatment.Patients in the treatment group received additional acupoint injection with salmon calcitonin at bilateral Pishu(BL 20)and Shenshu(BL 23),while patients in the control group received additional intramuscular injection with salmon calcitonin.The treatments for both groups were given once a day and lasted for 4 weeks.Visual analog scale(VAS)and Chinese Oswestry disability index(CODI)scores were observed before treatment,after 2 weeks and 4 weeks of treatment,and the use of analgesics during the treatment were recorded.Results:After treatment,the clinical efficacy in the treatment group was more significant than that in the control group(P<0.05).After 2 weeks and 4 weeks of treatment,the VAS scores in both groups showed significant intra-group and between-group differences(all P<0.05),and the CODI scores in both groups showed significant intra-group differences(all P<0.05).After 2 weeks of treatment,the CODI score showed no significant between-group difference(P>0.05).After 4 weeks of treatment,the improvement of CODI score in the treatment group was more significant than that in the control group(P<0.05).During the treatment,2 cases in the treatment group took analgesics versus 8 cases in the control group,and the result showed a significant between-group difference(P<0.05).Conclusion:For back pain in elderly patients with primary osteoporosis,based on the routine treatment of oral medication,the clinical efficacy of acupoint injection with salmon calcitonin at bilateral Pishu(BL 20)and Shenshu(BL 23)is more significant than that of intramuscular injection.Acupoint injection treatment can improve patients'conditions and reduce the use of analgesics.
文摘Objective:To observe the effects of moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)on the energy metabolism and endocrine metabolism indicators of rats undergoing one-time exhaustive swimming,and to explore the differences between moxibustion at different points in the effects on anti-exercise fatigue.Methods:Forty-eight male SPF rats were randomly divided into a blank group,a model group,a non-meridian and non-acupoint group,a Shenshu(BL 23)group,a Zusanli(ST 36)group,and a Shenque(CV 8)group using random number table method,with eight rats in each group.Except for the blank group,rats in the other groups were subjected to replicating the one-time exhaustive model using the weight-bearing swimming experiment.Except for the model group,the other model rats received mild moxibustion immediately after swimming.Rats in the non-meridian and non-acupoint group received mild moxibustion at bilateral subcostal non-meridian and non-acupoint points,those in the Shenshu(BL 23)group received mild moxibustion at bilateral Shenshu(BL 23),those in the Zusanli(ST 36)group received mild moxibustion at bilateral Zusanli(ST 36),and those in the Shenque(CV 8)group received mild moxibustion at Shenque(CV 8)for 15 min.Four hours after the exhaustive swimming,femoral artery blood was collected to detect blood lactate(BLA),lactate dehydrogenase(LDH),creatine kinase(CK),creatinine(CRE),blood urea nitrogen(BUN),cortisol(C)and testosterone(T)levels,and calculate the T/C ratio.Results:Compared with the blank group,rat's serum levels of BLA,LDH,CK,BUN and C in the model group and the non-meridian and non-acupoint group were increased,and serum levels of CRE and T,and T/C ratios were decreased(P<0.01 or P<0.05);compared with the model group and the non-meridian and non-acupoint group,the serum levels of BLA,LDH,CK,BUN and C in the Shenshu(BL 23)group,Zusanli(ST 36)group and Shenque(CV 8)group were decreased,and the serum CRE and T levels,and the T/C ratios were increased(all P<0.01);compared with the Shenshu(BL 23)group,the serum CK level was decreased in the Shenque(CV 8)group(P<0.01),the serum levels of T and C were decreased in the Zusanli(ST 36)group and Shenque(CV 8)group(P<0.01 or P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.01);compared with the Zusanli(ST 36)group,the serum CK and BUN levels were decreased(P<0.01,P<0.05),and the T/C ratio was increased in the Shenque(CV 8)group(P<0.05).Conclusion:Moxibustion at Shenshu(BL 23),Zusanli(ST 36)and Shenque(CV 8)shows different anti-fatigue effects by regulating the energy metabolism and endocrine metabolism in rats undergoing one-time exhaustive swimming.Moxibustion at Shenshu(BL 23)is better in promoting energy synthesis.Moxibustion at Shenque(CV 8)is more effective in regulating synthesis and decomposition of the skeletal muscle proteins.
基金supported by the Open Fund for Colleges and Universities Innovation Platform of Hunan Province,No.14K070Key Project of Hunan Province Administration of Traditional Chinese Medicine,No.201310Innovation Fund Project for Graduate Student of Hunan Province,No.CX2014B360~~
文摘Objective: To observe the effect of electroacupuncture (EA) on the expression of erythropoie-tin-producing hepatocyte receptor B2 (EphB2) in the cortex around the infracted area of middle cerebral artery occlusion (MCAO) rats at different timing, and to reveal the possible mechanism of acupuncture in the treatment of cerebral ischemia. Methods: A total of 180 male Sprague-Dawley (SD) rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, with 45 rats in each group. Rats in each group were further divided into three subgroups: postoperative 3 d, postoperative 14 d and postoperative 2l d groups, with 15 rats in each subgroup. The MCAO model was made by the modified occlusion method. The neurological function score, 2,3,5-triphenyl tetrazolium chloride (TFC) staining, immunohistochemistry assay, immunofluorescence double labeling method and Western blot were used to detect the corresponding indicators. Results: The neurological impairment of rats was most obvious at postoperative 3 d, and then gradually improved with time, which was more significant in the acupoint group (P〈0.05). The change of infarcted volume was consistent with the neurological function impairment. The number of EphB2 positive cells (EphB2+) around the infarcted area was decreased significantly at postoperative 3 d, and then gradually improved with time, which returned to the same level as that in the sham operation group at postoperative 21 d. The increase was most significant in the acupoint group (P〈0.05), and the positive cell number was higher than that in the sham operation group (P〈0.01). Western blot and immunohistochemistry results were basically consistent. Immunofluorescence displayed that EphB2+ and postsynaptic density-95 positive (PSD-95+) were co-expressed, after the MCAO operation, in the cortical neuron around the infracted area, and the number of co-expressing cells was increased gradually with time, which was most significant in the acupoint group (P〈0.05). Conclusion: Electroacupuncture at Ganshu (BL 18) and Shenshu (BL 23) can significantly improve the neurological function and cerebral infarcted volume ratio of MCAO rats, which may be related to the activation of EphB2 expression in cortex around the infracted area and the promotion of synaptic remodeling.
文摘【目的】观察电针百会、风府、双侧肾俞穴联合人脐带间充质干细胞(hUC-MSCs)移植对缺血性脑损伤大鼠的脑保护作用及机制。【方法】将40只SD大鼠随机分为正常组、模型组、移植组、联合组,每组10只。除正常组,其他各组大鼠建立脑缺血再灌注损伤模型。在造模结束24 h后,移植组大鼠给予0.5 m L 2×10^(6)个hUC-MSCs细胞悬液一次性尾静脉植入,联合组在移植组治疗基础上,给予电针百会穴、风府穴、双侧肾俞穴,每次30 min,每日1次,连续针刺3周。治疗结束后,苏木素-伊红(HE)染色法观察海马组织病理形态,脱氧核糖核苷酸末端转移酶介导的缺口末端标记(TUNEL)法观察脑组织细胞凋亡情况,免疫荧光法检测脑组织腺苷A2A受体(ADORA2A)表达,免疫组织化学法检测脑组织糖原合酶激酶3β(GSK-3β)、β-连环蛋白(β-catenin)表达,Western Blot法检测脑组织跨膜蛋白闭锁蛋白(Occludin)、胞质附着蛋白(ZO-1)表达。【结果】与正常组比较,模型组脑组织细胞凋亡率升高,脑组织ADORA2A阳性表达率及GSK-3β蛋白表达水平升高,Occludin、ZO-1、β-catenin蛋白表达水平降低(P<0.05),HE染色结果显示,模型组海马组织结构明显异常;与模型组比较,移植组和联合组大鼠脑组织细胞凋亡率降低,脑组织ADORA2A阳性表达率及GSK-3β蛋白表达水平降低,Occludin、ZO-1、β-catenin蛋白表达水平升高(P<0.05),海马组织病理程度明显减轻;与移植组比较,联合组脑组织细胞凋亡率降低,脑组织ADORA2A阳性表达率及GSK-3β蛋白表达水平降低,Occludin、ZO-1、β-catenin蛋白表达水平升高(P<0.05)。【结论】电针百会、风府、双侧肾俞穴联合hUC-MSCs可改善缺血性脑损伤大鼠血脑屏障,抑制脑组织ADORA2A、GSK-3β表达,提高β-catenin表达,抑制脑组织细胞凋亡,起到脑保护作用,且作用效果优于单纯hUC-MSCs移植。
基金supported by National Natural Science Foundation of China(No.81303029)National Basic Research Program of China(973 Program,No.2009CB522900)Natural Science Foundation of Shanghai(No.12ZR1429700)~~
文摘Objective:To observe the effect of moxibustion on learning and memory abilities,corticosterone and glucocorticoid receptor(GR) in subacute aging rats.Methods:Twenty four Sprague-Dawley(SD) rats were randomly divided into a normal group,a model group and a moxibustion group,8 rats in each group.Rats in the model group and the moxibustion group were subcutaneously injected with 25% D-galactose [125 mg/(kg·bw)] for 40 d continuous;rats in the normal group were injected with saline at the same position for 40 d continuous.Rats in the moxibustion group were given mild moxibustion at bilateral Shenshu(BL 23) at the same time of modeling;rats in the normal group and the model group were only identically grabbed without moxibustion for 40 d.The learning and memory abilities of rats were observed using the Morris water maze at the end of the experiment.Abdominal aorta blood and thymus were collected after water maze experiment.Enzyme-linked immunosorbent assay(ELISA) was used to detect serum corticosterone level,and immunohistochemical method was used to detect the expression of thymus GR.Results:Compared with the normal group,rats in the model group showed that a significantly longer escape latency time(P〈0.01) on the third and the fourth days;number of times crossing the platform in 70 s significantly reduced(P〈0.01);activity times in the fourth quadrant significantly decreased(P〈0.05);serum corticosterone levels increased(P〈0.01);thymus GR expression decreased(P〈0.05).Compared with the model group,rats in the moxibustion group showed that the escape latency times were significantly shorter on the third,the fourth and the fifth days(P〈0.01,P〈0.05);number of times crossing the platform in 70 s significantly increased(P〈0.05);activity times in the fourth quadrant significantly increased(P〈0.05);serum corticosterone levels decreased(P〈0.05);thymus GR expression increased(P〈0.05).Conclusion:Moxibustion could improve the learning and memory abilities of subacute aging rats,down-regulate serum corticosterone levels,and increase thymus GR content.
文摘OBJECTIVE:To examine whether specific stimulation of Shenshu(BL23)affects sympathetic nervous activity(SNA)-associated plasma renin concentration(PRC).METHODS:Eight healthy volunteers participated in three pattern conditions in random order:control(Cont),stimulation of Shenshu(BL23),and stimulation of sham point(Sham).All participants were initially in the supine position for>60 min,and then remained in the standing position during the experimental procedure to increase SNA.An electrocardiogram was used to calculate low frequency/high frequency(LF/HF)ratio;blood was collected to analyze PRC.RESULTS:The LF/HF ratio was significantly increased in the standing position when compared with the supine position(P<0.01).There was no difference in LF/HF ratio during or after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,the LF/HF ratio was significantly increased in Cont and Sham conditions(P<0.01).There was no difference in PRC after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,there was a significant increase in PRC in the Cont and Sham conditions(Cont P<0.05,Sham P<0.01).CONCLUSION:Our results demonstrated that specific acupuncture stimulation of Shenshu(BL23)in the standing position decreased SNA-associated PRC,which was not observed during acupuncture stimulation of the sham point.
文摘OBJECTIVE: It is unknown whether the psoas major muscle, thought to be a key muscle for treatment of lower back pain, can be punctured at Shenshu(BL23).METHODS: Twelve dissected specimens were used for studying the needling pathway of BL23 by perpendicularly inserting the depth-measuring blade of a vernier caliper at BL23. Dimensions of psoas muscle were measured. Correlation studies were conducted. In addition, our samples were grouped by gender and underlying medical conditions for analysis.RESULTS: Half(50%) of the needle insertions successfully punctured psoas muscle. The mean depth of needle insertion to puncture psoas muscle(D_(min)) in the group with short-term underlying medical conditions was 38.0 mm(interquartile range 29.0–51.8 mm), approximately 6 mm deeper than 32.0(29.3–42.5) mm in the group with long-term health problems(P = 0.041). The cross-sectional area(CSA) of psoas muscle in the former group was on average approximately 1.5 times that of the latter group(P = 0.04). When the data were analysed by gender, the thickness of psoas muscle in the male group was 19.0(6.5–24.0) mm compared to 19.5(5.8–34.8) mm in the female group(P = 0.02). The age in the female group(P = 0.04) and the body length of the total group(P = 0.04) negatively correlated to D_(min).CONCLUSION: Needle insertion at BL23 might be able to puncture psoas muscle. Differences in the CSA of psoas muscle and D_(min) were observed in groups with short-term and long-term underlying medical conditions.