Effect of electroacupuncture at Jiaji (EX-B2) points on the expression of microRNA-21 and neuronal apoptosis in rats with acute spinal cord injury

Objective:To observe the effect of electroacupuncture at Jiaji (EX-B2) points on the expression of microRNA-21 and neuronal apoptosis in rats with acute spinal cord injury. Methods:SD rats were randomly divided into sham-operated group, model group, electro-acupuncture group and methylprednisolone group. The model of acute spinal cord injury was reproduced by modified Allen's method. Each group was given corresponding intervention treatment 2 hours after modeling. The electroacupuncture group was treated with electro-acupuncture at Jiaji (EX-B2) points in T8 to T12, the methylprednisolone group was given intraperitoneal injection of 30 mg/kg methylprednisolone sodium succinate, and the sham operation group and model group were not treated. After treatment, BBB scores were observed to evaluate the motor function of posterior limb in each group, and the injured spinal cord tissues were taken. The pathological changes of spinal cord neuropathy were observed by Nissl staining. The expression of microRNA-21 was detected by RT-qPCR, and the expression of apoptotic proteins (Bax, Bcl-2 and cleaved-Caspase-3) was detected by Western-Blot. Result:Compared with sham operation group, BBB, neuron survival rate and expression of microRNA-21 were significantly lower, apoptotic rate of neurons was significantly higher, expression of Bax, Bcl-2, cleaved Caspase-3 protein and Bax/Bcl-2 ratio were significantly increased in model group (P< 0.05). Electroacupuncture could significantly increase BBB score, neuron survival rate and expression of microRNA-21, reduce apoptotic rate of neurons and the ratio of Bax/Bcl-2, and inhibit the expression of Bax, Bcl-2 and cleaved Caspase-3 protein in tissues, which were significantly different from those of model group (P< 0.05). Conclusions: Electroacupuncture at Jiaji (EX-B2) points can significantly promote the recovery of neurological function in rats after ASCI, and its mechanism may be closely related to the up-regulation of the expression of microRNA-21 in tissues and the inhibition of the activation of Bax/Bcl-2/cleaved Caspase-3 signaling pathway.

THE TREATMENT OF CERVICAL SPONDYLOPATHY BY LASER ACUPUNCTURE AT THE CERVICAL JIAJI POINTS: AN ANALYSIS OF THERAPEUTIC EFFECT OF 102 CASES

Laser acupuncture therapy was employed in the treatment of spondylopathy.Thetherapeutic effect of the three types of the spondylopathy,nerve root type,vertebral artery type andspinal cord type were stuied and compared.The results indicate a significant difference between thethree types of spondylopathy.The best therapeutic effect was obtained in nerve root type with a remarkable effective rate of 98.5％;the effective rates for the vertebral artery type and the spinal cordtype were 92.3％and 25％,respectively.Combining laser irradiatlon with acupuncture,the therapyposseses the functions of acupuncture,heat effect and mild moxibustion action.Therefore,it showedeffect quickly and had the advantages of short treatment course and high therapeutic effect.Clinicalobservation has confirmed that laser acupuncture is more effective than single laser irradiation.Thistherapy offers another simple but effective method for the treatment of spondylopathy.

电针夹脊穴联合祛风骨痛凝胶膏治疗2型糖尿病足气虚血瘀证临床研究

目的:观察电针夹脊穴联合祛风骨痛凝胶膏治疗2型糖尿病足气虚血瘀证患者的临床效果。方法:选择2型糖尿病足气虚血瘀证患者80例,以随机数字表法分为对照组(n=40)、治疗组(n=40)。治疗组采用电针夹脊穴联合祛风骨痛凝胶膏治疗;对照组应用电针夹脊穴治疗。比较两组患者临床症状积分、血液流变学指标、微循环血流情况。结果:治疗组的总有效率92.50%高于对照组的总有效率87.50%,差异有统计学意义(P<0.05)。治疗4周后,两组患者皮肤温度、疼痛程度、麻木程度、皮肤色泽、间歇性跛行距离的积分均较治疗前降低,且治疗组低于对照组,差异有统计学意义(均P<0.05)。治疗组血液流变学指标低于对照组,两组足背温度、温控血流、阻断后反应性充血、经皮氧分压均高于治疗前,且治疗组高于对照组,差异有统计学意义(均P<0.05)。结论:电针夹脊穴联合祛风骨痛凝胶膏能有效改善2型糖尿病足气虚血瘀证患者的临床症状,降低血液黏度,改善微循环血流状态,临床应用安全、作用持久。

Effectiveness of electroacupuncture at Jiaji acupoints(EX-B 2), plus moxibustion and intermediate on postherpetic neuralgia: a randomized controlled trial

OBJECTIVE:To investigate the effectiveness of electroacupuncture at Jiaji acupoints(EX-B 2)plus moxibustion and intermediate frequency on postherpetic neuralgia(PHN).METHODS:A total of 140 outpatients who satisfied the inclusion criteria and volunteered for this treatment were randomly divided into treatment(n=70)and control(n=70)groups.Both groups received a localized lesion area and electroacupuncture treatment combined with moxibustion and intermediate frequency.The treatment group(TG)increased acupuncture at Jiaji acupoints(EX-B 2)and electroacupuncture.Pain and anxiety were assessed before and after 5,10,15,and 20 treatments by using visual pain simulation score(VAS)and Hamilton anxiety scale(HAMA),respectively.Clinical efficacy was also evaluated.RESULTS:The baseline between the two groups did not significantly differ(P>0.05).The VAS and HAMA scores of the two groups after treatment significantly decreased compared with those of various treatment stages(P>0.05).The HAMA score(P<0.01)of TG was lower than that of the control group(CG).The VAS score of TG was lower than that of CG in the 5th and 10th treatments(P<0.01).In the 15 th and 10 th scores,CG was also superior to TG(P<0.05).CONCLUSION:The combined treatment of electroacupuncture at Jiaji acupoints(EX-B 2),moxibustion,and intermediate frequency can relieve the pain and anxiety symptoms of PHN.The efficacy of the combined treatment was superior to traditional acupuncture.

Clinical observation on acupuncture at Jiaji(EX-B 2) points plus tuina in treating thoracic facet joint disorder

Objective： To observe the clinical effects of acupuncture at Jiaji （EX-B 2） points plus tuina manipulation for thoracic facet joint disorder, and get new clinical evidence for treatment of thoracic facet joint disorder. Methods： Totally 106 patients with thoracic facet joint disorder were randomly allocated into an observation group and a control group based on the random number table. Patients in the control group were treated by tuina manipulation, while those in the observation group were treated by acupuncture at Jiaji （EX-B 2） points before tuina manipulation. Patients in the two groups were treated once a day. The improvements of signs and symptoms and the efficacy were observed after 3 treatments. Results： After treatment, there were intra-group statistical differences in scores of 8 signs and symptoms in both groups （P〈0.01}; the score of each item in the observation group was lower than that in the control group, and there was statistical significance in the inter-group difference （P〈0.05）. The cure rate of the observation group was 50.9%, versus 26.4% in the control group, and there was a significant difference between the two groups （P〈0.05）. Conclusion： In treating thoracic facet joint disorder, acupuncture plus tuina manipulation can restore the biomechanical balance of thoracic vertebrae, fully maximized the combined effect, and significantly improve the clinical efficacy.

Acupuncture-moxibustion at Jiaji(EX-B 2) points for intervertebral disc herniation: a systematic review

By organizing and classifying the literatures related to acupuncture-moxibustion at Jiaji （EX-B 2） points in treating intervertebral disc herniation （IDH） published before December 32, 2013, to summarize the application status and features of Jiaji （EX-B 2） points. It＇s found that its most favorable indication is IDH. It also further analyzed the action mechanism of acupuncture-moxibustion at Jiaji （EX-B 2） points in treating IDH, and brought up some ideas to highlight the further study.

Observation on Therapeutic Effects of Electroacupuncture at Jiaji (Ex-B 2) Points plus Herbal Medicine for Herpes Zoster

Objective： To observe the clinical effects of electroacupuncture （EA） at Jiaji （Ex-B 2） points plus herbal medicine for herpes zoster （HZ）. Methods： Sixty-two cases were randomly divided into a treatment group and a control group. The treatment group was treated with EA at Jiaji （Ex-B 2） points plus herbal medicine based upon the pattern differentiation. The control group was treated with oral administration of Aciclovir tablets for resisting virus, and Mecobalamin tablets for nourishing the nerves and Aciclovir cream for skin rash. The therapeutic effects were compared between the two groups, after two-week treatment Results： After 7 d of the treatment, the therapeutic effect was remarkably better in the treatment group than that in the control group （P〈0.01）. After two-week treatment, the therapeutic effects were still different between the two groups （P〈0.05）. Conclusion： The therapeutic effect of EA at Jiaji （Ex-B 2） points plus herbal medicine for HZ is better than that of Western medications in resisting virus and nourishing nerves.

Treatment of 100 Cases of Cervical Spondylotic Radiculopathy by Electroacupuncture of Jiaji(Ex-B2)plus Oblique Needling Ashi Point

Summary： One hundred patients were diagnosed with cervicalspondylotic radiculopathy definitely by symptoms, signs and Xray examination. The therapeutic method was acupuncture of Jiaji（Ex-B 2） on the affected segment and the upper and lower vertebrae close together. After the arrival of qi, the needles were connected to acupuncture therapeutic instrument. Meanwhile, the numb, heavy and uncomfortable area on the shoulder and upper limb, where something like cord could be touched, was selected as Ashi point. The needle was inserted perpendicularly into the skin on the place that the cord was most obvious and inserted obliquely and deeply along the cord. After 3 4 courses of treatment, 90 cases got marked effectiveness and 10 cases got effectiveness.

针刺夹脊穴为主治疗2型糖尿病胃轻瘫的临床观察

目的:针刺夹脊穴为主治疗2型糖尿病胃轻瘫(DGP)的临床效果。方法:选取2015年3月至2016年2月汕头潮阳区大峰医院60例2型糖尿病胃轻瘫患者,随机分为观察组和对照组,每组各30例。对照组接受莫沙必利口服,观察组接受T5～T12夹脊穴为主穴的针刺治疗,疗程为30 d。以疗效指数、症状积分改善情况和不良反应为观察指标,并进行统计学处理。结果:(1)经治疗后,观察组总有效率达100.0%,对照组达93.3%,两组比较,差异具有统计学意义(P<0.05)。(2)两组患者治疗后餐后饱胀不适、早饱、上腹痛、上腹烧灼感的症状积分和症状总积分较治疗前显著改善,差异具有统计学意义(P<0.05);且治疗后两组患者上腹痛、上腹烧灼感症状积分和总症状积分比较,差异具有统计学意义(P<0.05)。(3)观察组中,3例患者先后出现针刺穴位处血肿,未经处理,血肿自行吸收,其余未见不良反应。结论:以夹脊穴为主穴的针刺疗法对DGP有较好的近期效果,可以明显减轻患者的症状,特别对症状上腹痛和上腹烧灼感的改善,优于莫沙必利。

TREATMENT OF 29 CASES OF LUMBAR INTERVETEBRAL DISC PROTRUSION WITH GINGER-SEPARATED MOXIBUSTION

: To observe the effect of ginger-separated moxibustion for treatment of lumbar intervertebral disc prolapse. Methods: 29 lumbar intervertebral disc protrusion patients including 21 males and 8 females were treated with ginger-separated moxibustion of Ashi-point and Jiaji (EX-B 2 , the affected region), 5-6 cones every time, once every other day, continuously for 5 times. Then the therapeutic effect was analyzed. Results: After treatment, of the 29 cases, 23(79.3%) were cured, and the rest 6(20.7%) experienced improvement. Conclusion: Ginger-separated moxibustion is fairly effective and safe in treatment of lumbar intervertebral disc protrusion.

Intractable post-herpetic neuralgia in the trunk treated with point injection at Jiájǐ(夹脊 EX-B 2)

ABSTRACT Objective To compare the efficacy difference between point injection at Jiiaji （夹脊 EX-B 2） and oral medication in the treatment of intractable post-herpetic neuralgia （PHN） in the trunk. Methods One hundred and thirty cases were randomly divided into an point injection group and an medication group, 65 cases in each one. In point injection group, according to the invasion site of herpes zoster, Jiiaji （夹脊 EX-B 2） of corresponding segments and the meridian points on skin lesion areas, such as Fengchi （风池GB 20）, Tianzhu （天柱 BL 10）, Feishu （肺俞 BL 13） and Geguan （膈关 BL 46） were selected and injected with the mixed solution of compound Betamethasone （administered only in the first injection）, Vitamin B12 and Lidoeaine Carbonate, 2 mL on each point, once per day. In medication group, Diclofenac Sodium sustained release tablets, 75 mg were administered, twice per day. The clinical efficacy was compared 10 days later between two groups. Results The total effective rate in point injection group was 100.0% （65/65）, which was better than 66.1% （43/65） in medication group （P〈0.01）. Conclusion Point injection mainly at Jiiaji （夹脊 EX-B 2） is effective significantly on intractable PHN in the trunk, which is superior to Diclofenac Sodium sustained release tablets.

Effects of electroacupuncture at “Jiaji ” (EX-B 2) on myocardial apoptosis and the expression of Bcl-2 and Bax in rats with ischemia-reperfusion injury

Objective To explore the protective mechanism of acupuncture on myocardial ischemia-reperfusion injury.Methods Fifty Wistar rats were randomly divided into a sham-operation group,a model group,a Jiaji group,a Neiguan group and a Yanglingquan group,10-rats

针刺颈夹脊穴调节颈椎病所致椎动脉血液动力学紊乱的即刻与近期效应

目的 :评价针刺疗程对椎动脉血液动力学的影响。方法 :对 32例椎动脉血液动力学紊乱的颈椎病患者 ,用TCD检测针刺颈夹脊穴的即刻与近期效应。 结果 :近期效应明显优于即刻效应(TCD参数Vp、Vm、Vd均P <0 0 1)。结论

针刺颈椎夹脊穴治疗椎-基底动脉供血不足综合征疗效观察

目的 :观察比较针刺颈椎夹脊穴和药物治疗椎 基底动脉供血不足综合征的临床疗效。方法 :临床随机分为针刺组 5 5例 ,药物组 4 2例。针刺组采用针刺颈椎夹脊穴 ,药物组静滴丹参注射液、口服太极通天液和西比灵。结果 :针刺组治愈率为 5 2 7% ,总有效率为 96 3% ;药物组治愈率为2 8 6 % ,总有效率为 73 8%。经统计学处理 ,两组疗效差异具有非常显著性意义 (P <0 .0 1)。 结论

Spinal stenosis treated with acupuncture on Huátuó Jiájǐ(夹脊EX-B 2) and vertebral curvature adjustment:report of therapeutic effect on 189 cases

Objective To observe the clinical effects on spinal stenosis treated with acupuncture on Huatuo Jiaji （夹脊EX-B 2） and vertebral curvature adjustment. Methods Comprehensive therapy was applied to all of 189 cases of any type of spinal stenosis, in which gukong needling technique was applied to Huatuo Jiaji （夹脊EX-B 2） at C4 - T5 and T12 - L5, associated with vertebral curvature adjustment with traction. The therapeutic effects were observed. Results Total effective rate was 96.8%, in which, clinical cured rate was 31.7% and excellent and good rate was 89.9% in average 28-month fol- low-up visit. Conclusion Spinal stenosis is segmental dynamic stenosis. It can be cured by acupuncture on Huatuo Jiaji （夹脊EX-B 2） and vertebral curvature adjustment and satisfactory result is achieved.

夹脊穴为主治疗腰椎骨质增生症的疗效评价

目的:评价针刺夹脊穴为主治疗腰椎骨质增生症的疗效.方法:72例病人以针刺L2～L5华佗夹脊穴为主,治疗前后均用Oswestry功能障碍指数(ODI)评分并进行临床疗效评定.结果:治疗前患者ODI评分为0.562±0.199治疗后为0.123±0.196,治疗前后比较差异有非常显著性意义(P＜0.01).72例中临床治愈36例,好转32例,有效率为94.4%.结论:夹脊穴治疗腰椎骨质增生症能明显改善患者功能活动状况.

针刺头部穴配合夹脊治疗慢性疲劳综合征疗效观察

目的观察针刺头部穴配合夹脊治疗慢性疲劳综合征的临床疗效。方法将70例慢性疲劳综合征患者随机分为治疗组和对照组,每组35例。治疗组采用针刺头部穴配合夹脊治疗,对照组采用常规针刺治疗。观察两组治疗前后疲劳量表-14评分,并比较两组临床疗效。结果两组治疗后疲劳量表-14评分与同组治疗前比较,差异均具有统计学意义(P<0.01)。治疗组治疗后疲劳量表-14评分与对照组比较,差异具有统计学意义(P<0.05)。治疗组总有效率和愈显率分别为91.4%和71.4%,对照组分别为82.9%和45.7%。两组愈显率比较,差异具有统计学意义(P<0.05)。结论针刺头部穴配合夹脊是一种治疗慢性疲劳综合征的有效方法。

颈夹脊穴针刺深度对颈椎病疗效的影响

目的观察颈夹脊穴针刺不同深度对颈椎病疗效的影响。方法将60例颈椎病患者随机分为治疗组和对照组,每组30例。两组均采用针刺颈夹脊穴治疗,治疗组直刺0.3～0.6寸,对照组直刺1.0～1.3寸。比较两组临床疗效。结果两组治疗后颈椎功能评分及总有效率比较,差异均无统计学意义(P>0.05)。结论在得气的基础上,颈夹脊穴针刺深度对颈椎病的疗效无显著性差异。

夹脊穴电针配合走罐治疗单纯性肥胖症疗效观察

目的观察夹脊穴电针配合走罐治疗单纯性肥胖症患者近远期的临床疗效,探讨针刺治疗单纯性肥胖的疗程与疗效的关系。方法 67例单纯性肥胖症患者均采用夹脊穴电针配合走罐治疗,1个月为1个疗程,治疗3个疗程后比较患者治疗前后及治疗后1年各项肥胖指标。结果患者治疗后及治疗后1年各项肥胖指标和生化指标与治疗前比较,差异均具有统计学意义(P<0.05)。结论夹脊穴电针配合走罐是一种治疗单纯性肥胖的有效方法。

穴位注射夹脊穴加针刺治疗老年带状疱疹后遗神经痛疗效观察

方法 :采用夹脊穴注射药物加毫针围刺病损治疗老年带状疱疹后遗神经痛 80例 ,并与针刺治疗 2 8例及西药治疗 30例进行比较。结果 :前者治愈率为 81 2 % ,总有效率为 10 0 0 % ,后两者分别为 32 1%、78 6 %和 33 3%、80 0 %。前者与后两者之间分别相比较 ,差异有非常显著性意义 ,P <0 0 1,前者疗效明显优于后两者。结论 :本法治疗老年带状疱疹后遗神经痛疗效较好。