Background: To explore the effects of electroacupuncture on cardiac function and myocardial fibrosis in rat models of heart failure, and to elucidate the underlying mechanism of electroacupuncture in heart failure tre...Background: To explore the effects of electroacupuncture on cardiac function and myocardial fibrosis in rat models of heart failure, and to elucidate the underlying mechanism of electroacupuncture in heart failure treatment. Methods: Healthy male Sprague-Dawley rats were allocated into three groups: Sham group, Model group, and electroacupuncture (Model + EA) group, with each group comprising 8 rats. The model underwent a procedure involving the ligation of the left anterior descending coronary artery to induce a model of heart failure. The Model + EA group was used for 7 consecutive days for electroacupuncture of bilateral Shenmen (HT7) and Tongli (HT5), once a day for 30 min each time. Left ventricular parameters in rats were assessed using a small-animal ultrasound machine to analyze changes in left ventricular end-diastolic volume, left ventricular end-systolic volume, left ventricular ejection fraction, and left ventricular fractional shortening. Serum interleukin-1β (IL-1β), cardiac troponin (cTn), and N-terminal brain natriuretic peptide precursor levels were measured using ELISA. Histopathological changes in rat myocardium were observed through HE staining, while collagen deposition in rat myocardial tissue was assessed using the Masson staining method. Picro sirius red staining, immunohistochemical staining, and RT-qPCR were utilized to distinguish between the various types of collagen deposition. The expression level of TGF-β1 and SMAD2/3/4/7 mRNA in rat myocardial tissues was determined using RT-qPCR. Additionally, western blot analysis was conducted to assess the protein expression levels of TGF-β1, SMAD3/7, and p-SMAD3 in rat myocardial tissues. Results: Compared with the Sham group, the left ventricular ejection fraction and left ventricular fractional shortening values of the Model group were significantly decreased (P < 0.01);the left ventricular end-diastolic volume and left ventricular end-systolic volume values were remarkably increased (P < 0.01);serum N-terminal brain natriuretic peptide precursor content was increased (P < 0.01);serum IL-1β and cTn levels were increased (P < 0.01);myocardial collagen volume fraction were increased (P < 0.01);and those of the expression of TGF-β1 and SMAD2/3/4 mRNA was increased (P < 0.01);the expression of SMAD7 mRNA was decreased (P < 0.01);the protein expression levels of TGF-β1, SMAD3, and p-Smad3 were increased (P < 0.01);the protein expression level of SMAD7 was decreased (P < 0.01) in the Model group. Compared to the Model group, the expression levels of the proteins TGF-β1, SMAD3, and p-Smad3 in myocardial tissue were found to be decreased (P < 0.01), and the expression level of the protein SMAD7 was found to be increased (P < 0.01) in the Model + EA group;the collagen volume fraction and deposition of type Ⅰ /Ⅲ collagen were decreased (P < 0.01) in the Model + EA group. Conclusion: Electroacupuncture alleviates myocardial fibrosis in rats with heart failure, and this effect is likely due to attributed to the modulation of the TGF-β1/Smads signaling pathway, which helps reduce collagen deposition in the extracellular matrix.展开更多
目的探讨子午流注联合穴位贴敷治疗冠心病心绞痛的临床疗效。方法收集2018年12月—2019年6月期间在沈阳市中医院心病科治疗的患者100例,采用随机数表法随机分为对照组和治疗组。对照组患者进行常规的西医基础性治疗,嘱患者低盐低脂饮食...目的探讨子午流注联合穴位贴敷治疗冠心病心绞痛的临床疗效。方法收集2018年12月—2019年6月期间在沈阳市中医院心病科治疗的患者100例,采用随机数表法随机分为对照组和治疗组。对照组患者进行常规的西医基础性治疗,嘱患者低盐低脂饮食,予以0.9%氯化钠注射液50 m L/5%葡萄糖注射液50 m L+硝酸甘油注射液10 mg,3~5 mL/h静脉注射,并在西医治疗的基础上予以穴位贴敷治疗。治疗组在对照组西医治疗基础上选择每日午时(11:00~13:00)予以穴位贴敷治疗,1次/d,每次时间持续20 min。两组患者均进行20 d持续性治疗。观察两组患者治疗前后中医证候积分(主症)、Hcy、NO、hs-CRP和NF-kB水平及临床有效率。结果两组患者中医证候主症评分、Hcy、NO、hs-CRP及NF-kB含量较治疗前均有所改善,且治疗后治疗组明显优于对照组,差异有统计学意义(P<0.05);治疗后,对照组总有效率为66.0%,治疗组总有效率为90.0%,差异有统计学意义(P<0.05)。结论在西医治疗基础上联合子午流注穴位贴敷治疗冠心病心绞痛能够有效地抑制炎症反应,抗血小板聚集,延缓血栓形成速率,改善胸闷、胸痛等症状,提高临床有效率。展开更多
基金the China’s National Key Research and Development Program Projects(No.2022YFC3500500 and No.2022YFC3500502).
文摘Background: To explore the effects of electroacupuncture on cardiac function and myocardial fibrosis in rat models of heart failure, and to elucidate the underlying mechanism of electroacupuncture in heart failure treatment. Methods: Healthy male Sprague-Dawley rats were allocated into three groups: Sham group, Model group, and electroacupuncture (Model + EA) group, with each group comprising 8 rats. The model underwent a procedure involving the ligation of the left anterior descending coronary artery to induce a model of heart failure. The Model + EA group was used for 7 consecutive days for electroacupuncture of bilateral Shenmen (HT7) and Tongli (HT5), once a day for 30 min each time. Left ventricular parameters in rats were assessed using a small-animal ultrasound machine to analyze changes in left ventricular end-diastolic volume, left ventricular end-systolic volume, left ventricular ejection fraction, and left ventricular fractional shortening. Serum interleukin-1β (IL-1β), cardiac troponin (cTn), and N-terminal brain natriuretic peptide precursor levels were measured using ELISA. Histopathological changes in rat myocardium were observed through HE staining, while collagen deposition in rat myocardial tissue was assessed using the Masson staining method. Picro sirius red staining, immunohistochemical staining, and RT-qPCR were utilized to distinguish between the various types of collagen deposition. The expression level of TGF-β1 and SMAD2/3/4/7 mRNA in rat myocardial tissues was determined using RT-qPCR. Additionally, western blot analysis was conducted to assess the protein expression levels of TGF-β1, SMAD3/7, and p-SMAD3 in rat myocardial tissues. Results: Compared with the Sham group, the left ventricular ejection fraction and left ventricular fractional shortening values of the Model group were significantly decreased (P < 0.01);the left ventricular end-diastolic volume and left ventricular end-systolic volume values were remarkably increased (P < 0.01);serum N-terminal brain natriuretic peptide precursor content was increased (P < 0.01);serum IL-1β and cTn levels were increased (P < 0.01);myocardial collagen volume fraction were increased (P < 0.01);and those of the expression of TGF-β1 and SMAD2/3/4 mRNA was increased (P < 0.01);the expression of SMAD7 mRNA was decreased (P < 0.01);the protein expression levels of TGF-β1, SMAD3, and p-Smad3 were increased (P < 0.01);the protein expression level of SMAD7 was decreased (P < 0.01) in the Model group. Compared to the Model group, the expression levels of the proteins TGF-β1, SMAD3, and p-Smad3 in myocardial tissue were found to be decreased (P < 0.01), and the expression level of the protein SMAD7 was found to be increased (P < 0.01) in the Model + EA group;the collagen volume fraction and deposition of type Ⅰ /Ⅲ collagen were decreased (P < 0.01) in the Model + EA group. Conclusion: Electroacupuncture alleviates myocardial fibrosis in rats with heart failure, and this effect is likely due to attributed to the modulation of the TGF-β1/Smads signaling pathway, which helps reduce collagen deposition in the extracellular matrix.
文摘目的探讨子午流注联合穴位贴敷治疗冠心病心绞痛的临床疗效。方法收集2018年12月—2019年6月期间在沈阳市中医院心病科治疗的患者100例,采用随机数表法随机分为对照组和治疗组。对照组患者进行常规的西医基础性治疗,嘱患者低盐低脂饮食,予以0.9%氯化钠注射液50 m L/5%葡萄糖注射液50 m L+硝酸甘油注射液10 mg,3~5 mL/h静脉注射,并在西医治疗的基础上予以穴位贴敷治疗。治疗组在对照组西医治疗基础上选择每日午时(11:00~13:00)予以穴位贴敷治疗,1次/d,每次时间持续20 min。两组患者均进行20 d持续性治疗。观察两组患者治疗前后中医证候积分(主症)、Hcy、NO、hs-CRP和NF-kB水平及临床有效率。结果两组患者中医证候主症评分、Hcy、NO、hs-CRP及NF-kB含量较治疗前均有所改善,且治疗后治疗组明显优于对照组,差异有统计学意义(P<0.05);治疗后,对照组总有效率为66.0%,治疗组总有效率为90.0%,差异有统计学意义(P<0.05)。结论在西医治疗基础上联合子午流注穴位贴敷治疗冠心病心绞痛能够有效地抑制炎症反应,抗血小板聚集,延缓血栓形成速率,改善胸闷、胸痛等症状,提高临床有效率。
