The effects of different doses of swainonine（ SW）,thermopsine（ Ts） and their mixture on transformation of mouse T lymphocytes were studied,in order to figure out the poisoning mechanism of Oxytropis glabra. Totall...The effects of different doses of swainonine（ SW）,thermopsine（ Ts） and their mixture on transformation of mouse T lymphocytes were studied,in order to figure out the poisoning mechanism of Oxytropis glabra. Totally 240 mice were randomly grouped,and orally administrated with different concentrations of SW,Ts and their mixture. Four mice were randomly selected from each group at the 7th,14th,21st and 35th days,and lymphocytes were separated from anti-coagulated blood collected from eyeball. After Con A stimulation,T lymphocyte proliferation was detected by MTT assay. The results showed that low dose of SW promoted proliferation of mouse T lymphocyte; medium and high dose of SW also promoted proliferation of mouse T lymphocyte within a short period,but would seriously suppress its proliferation after a long period. Low dose of Ts almost had no impact on proliferation of T lymphocyte,while high dose also suppressed its proliferation.A similar result with SW was observed on alkaloid mixture. This indicated that different doses of SW,Ts and their mixture affected proliferation of T lymphocyte in different degrees.展开更多
基金Supported by Natural Science Foundation of Inner Mongolia(2012MS0404)
文摘The effects of different doses of swainonine（ SW）,thermopsine（ Ts） and their mixture on transformation of mouse T lymphocytes were studied,in order to figure out the poisoning mechanism of Oxytropis glabra. Totally 240 mice were randomly grouped,and orally administrated with different concentrations of SW,Ts and their mixture. Four mice were randomly selected from each group at the 7th,14th,21st and 35th days,and lymphocytes were separated from anti-coagulated blood collected from eyeball. After Con A stimulation,T lymphocyte proliferation was detected by MTT assay. The results showed that low dose of SW promoted proliferation of mouse T lymphocyte; medium and high dose of SW also promoted proliferation of mouse T lymphocyte within a short period,but would seriously suppress its proliferation after a long period. Low dose of Ts almost had no impact on proliferation of T lymphocyte,while high dose also suppressed its proliferation.A similar result with SW was observed on alkaloid mixture. This indicated that different doses of SW,Ts and their mixture affected proliferation of T lymphocyte in different degrees.
