The pot experiment was carried out in this study to further explore the impact of plant nutrient, slaked lime, pokeberry root powder on cadmium （Cd） absorption by rice, and to screen practical agronomic measures whi...The pot experiment was carried out in this study to further explore the impact of plant nutrient, slaked lime, pokeberry root powder on cadmium （Cd） absorption by rice, and to screen practical agronomic measures which could reduce the accumulation of cadmium. In the control treatment nothing but water was sprayed into rice, while in other treatments, plant nutrient, slaked lime and pokeberry root powder were sprayed on rice at the tillering stage, the heading stage and the dough stage, respectively. The plant samples and soil samples were collected after the harvest and then analyzed on aimed indexes. The results showed that Cd contents in rice plants were significantly decreased, total Cd contents in brown rice were significantly （P〈0.05） decreased after rice was sprayed with plant nutrient, slaked lime and pokeberry root powder at different growing stages. However, while rice was sprayed with the three materials all at the tillering stage, the heading stage and the dough stage, the total Cd contents in brown rice were decreased by a maximum reduction that was 53.4% to 57.5% below that of the control, which was signifcantly （P〈0.05） lower than other treatments. Meanwhile, the biggest reduction of Cd inrice sprayed with the three materials at the tillering stage was 19.6% to 35.1% below that of the control, which was signifcantly （P〈0.05） lower than other treatments. In conclusion, spraying plant nutrient, slaked lime and pokeberry root powder on rice at different growing stages could not only reduce the absorption accumulation of Cd by root system, but also change the activities of rice and physical and chemical properties of soil, and then inhibit the absorption of Cd by the root system and the transfer of Cd in the rice plant. Therefore, the multiple factors may be the main causes of reducing Cd contents in the brown rice when the three materials are sprayed on rice at different growing stages.展开更多
从美洲商陆(Phytolacca americana L.)夏天采集的2—4年生宿根中分离了二种抗真菌蛋白,称为PAFP-R_1和PAFP-R_2。分离程序包括用盐溶液提取,经CM-Sephadex离子交换层析、凝胶过滤层析和羟基磷灰石柱层析纯化。在PGA培养基上,0.1mg/ml蛋...从美洲商陆(Phytolacca americana L.)夏天采集的2—4年生宿根中分离了二种抗真菌蛋白,称为PAFP-R_1和PAFP-R_2。分离程序包括用盐溶液提取,经CM-Sephadex离子交换层析、凝胶过滤层析和羟基磷灰石柱层析纯化。在PGA培养基上,0.1mg/ml蛋白明显抑制木霉菌丝的生长;但对细菌的增殖,即使1mg/ml也无抑制作用。用SDS-PAGE测得二者的相对分子量各为13kD和15kD,单多肽链,等电点约为5.8。用酚-硫酸法未测出含糖。二种蛋白均高含半胱氨酸(19mol/mol)。用Edman降解法测得二者的N末端均为Ala。秋天采集的根中,这二种蛋白含量均很低,但富含由二条PAFP-R_1肽链以双硫键联结的二聚体,它无抗真菌活性。冬天宿根中抗真菌蛋白主要成分是Mr为17kD的单肽链蛋白。上述蛋白对于人红血球均无凝集活性,因此不是PWM的成分。以上结果说明,商陆根中有多种具抗真菌活性的蛋白,成分随季节发生变化,它们都是不含糖,高含半胱氨酸,分子量小于20kD的单肽链蛋白。展开更多
文摘The pot experiment was carried out in this study to further explore the impact of plant nutrient, slaked lime, pokeberry root powder on cadmium （Cd） absorption by rice, and to screen practical agronomic measures which could reduce the accumulation of cadmium. In the control treatment nothing but water was sprayed into rice, while in other treatments, plant nutrient, slaked lime and pokeberry root powder were sprayed on rice at the tillering stage, the heading stage and the dough stage, respectively. The plant samples and soil samples were collected after the harvest and then analyzed on aimed indexes. The results showed that Cd contents in rice plants were significantly decreased, total Cd contents in brown rice were significantly （P〈0.05） decreased after rice was sprayed with plant nutrient, slaked lime and pokeberry root powder at different growing stages. However, while rice was sprayed with the three materials all at the tillering stage, the heading stage and the dough stage, the total Cd contents in brown rice were decreased by a maximum reduction that was 53.4% to 57.5% below that of the control, which was signifcantly （P〈0.05） lower than other treatments. Meanwhile, the biggest reduction of Cd inrice sprayed with the three materials at the tillering stage was 19.6% to 35.1% below that of the control, which was signifcantly （P〈0.05） lower than other treatments. In conclusion, spraying plant nutrient, slaked lime and pokeberry root powder on rice at different growing stages could not only reduce the absorption accumulation of Cd by root system, but also change the activities of rice and physical and chemical properties of soil, and then inhibit the absorption of Cd by the root system and the transfer of Cd in the rice plant. Therefore, the multiple factors may be the main causes of reducing Cd contents in the brown rice when the three materials are sprayed on rice at different growing stages.
文摘从美洲商陆(Phytolacca americana L.)夏天采集的2—4年生宿根中分离了二种抗真菌蛋白,称为PAFP-R_1和PAFP-R_2。分离程序包括用盐溶液提取,经CM-Sephadex离子交换层析、凝胶过滤层析和羟基磷灰石柱层析纯化。在PGA培养基上,0.1mg/ml蛋白明显抑制木霉菌丝的生长;但对细菌的增殖,即使1mg/ml也无抑制作用。用SDS-PAGE测得二者的相对分子量各为13kD和15kD,单多肽链,等电点约为5.8。用酚-硫酸法未测出含糖。二种蛋白均高含半胱氨酸(19mol/mol)。用Edman降解法测得二者的N末端均为Ala。秋天采集的根中,这二种蛋白含量均很低,但富含由二条PAFP-R_1肽链以双硫键联结的二聚体,它无抗真菌活性。冬天宿根中抗真菌蛋白主要成分是Mr为17kD的单肽链蛋白。上述蛋白对于人红血球均无凝集活性,因此不是PWM的成分。以上结果说明,商陆根中有多种具抗真菌活性的蛋白,成分随季节发生变化,它们都是不含糖,高含半胱氨酸,分子量小于20kD的单肽链蛋白。
