To characterize and purify the allergen extracted from Amaranthus viridis pollen.Extracts of Amaranthus viridis pollen was prepared and analyzed by SDS-PAGE.The protein bands were visualized by staining the gel with C...To characterize and purify the allergen extracted from Amaranthus viridis pollen.Extracts of Amaranthus viridis pollen was prepared and analyzed by SDS-PAGE.The protein bands were visualized by staining the gel with Coomassis blue and analyzed by immuno-blotting with serum from 30 allergic patients and from 20 normal people.30 protein bands were detected in SDS-PAGE.2 of these bands with molecular weight of 75 kD a and 67 kD a,showed immumo-reactivity with IgE in the sera from patients with allergy to Amaranthus viridis pollen.The 75 kD a and 67 kD a protein was the major allergen.Meamwhile,Ion-exchange chromatography with DE-52 was used for purification of the antigens.The purified products were characterized by Westerm-blotting.Result from the Ion-exchange chromatography indicated that the pollen allergens(75 kD a and 67 kD a)from the Amaranthus viridis pollen were eluted in theⅠandⅡpeak.The study shows pollen extracts from Amaranthus viridis pollen was purified and characterized and the major allergen was 75 kD a and 67 kD a protein.展开更多
Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lac...Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lactic acid bacteria(LAB)were used to ferment Brassica napus BP for alleviating its allergenicity.Four novel allergens(glutaredoxin,oleosin-B2,catalase and lipase)were identified with significant decreases in LAB-fermented BP(FBP)than natural BP by proteomics.Meanwhile,metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP,indicating the degradation of LAB on allergens.Moreover,FBP showed alleviatory effects in BALB/c mice,which relieved pathological symptoms and lowered production of allergic mediators.Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity,which were closely correlated with the alleviation of allergic reactivity.These findings could contribute to the development and utilization of hypoallergenic BP products.展开更多
This study set out to look into the palyno-morphological characteristics of specific allergenic species from diverse plant families in Samarkand, Uzbekistan. Six different species i.e. Betula pendula, Fraxinus pennsyl...This study set out to look into the palyno-morphological characteristics of specific allergenic species from diverse plant families in Samarkand, Uzbekistan. Six different species i.e. Betula pendula, Fraxinus pennsylvanica, Magnolia × soulangeana, Pinus brutia var. eldarica, Populus alba, Quercus robur were collected, pressed, identified, and then examined under a microscope. Under light microscope, both quantitative and qualitative characteristics of the pollen grains were recorded, including pollen type, pollen size, pollen shape (polar and equatorial view), P/E ratio, mesocolpium distance, exine thickness, colpi type, colpi length, and width. It was found that a warm spring in Uzbekistan causes an early commencement of the vegetative and dusting phases, in contrast to the climatic circumstances of the researched entities’ motherland (cold spring). Studies in aeropalinology and morphology enable the resolution of numerous environmental issues. The findings highlighted the importance of pollen morphology as an identifying aid and showed how the tested species’ palynological characteristics varied. In order to promote future phylogenetic description of the Uzbekistan flora, the current study may help us better understand the palynomorphological traits of native plants. This essay only briefly and inadequately addresses these issues. The information gathered will be used to make an atlas of allergic plants in Samarkand and an electronic warning system for the general public about the spread of pollen from the city’s allergenic plants in the future.展开更多
基金National Natural Science Foundation of China(Grant No:81160372)Natural Science Foundation of Jiangxi(Grant No:0640002,2007GZY0536)+1 种基金Science and Technology Project supported by the Education Department of Jiangxi(Grant No:Ganjiaojizi[2007]303)Science and Technology Project of Ganzhou(Grant No:Ganshikefa[2009]31-119)
文摘To characterize and purify the allergen extracted from Amaranthus viridis pollen.Extracts of Amaranthus viridis pollen was prepared and analyzed by SDS-PAGE.The protein bands were visualized by staining the gel with Coomassis blue and analyzed by immuno-blotting with serum from 30 allergic patients and from 20 normal people.30 protein bands were detected in SDS-PAGE.2 of these bands with molecular weight of 75 kD a and 67 kD a,showed immumo-reactivity with IgE in the sera from patients with allergy to Amaranthus viridis pollen.The 75 kD a and 67 kD a protein was the major allergen.Meamwhile,Ion-exchange chromatography with DE-52 was used for purification of the antigens.The purified products were characterized by Westerm-blotting.Result from the Ion-exchange chromatography indicated that the pollen allergens(75 kD a and 67 kD a)from the Amaranthus viridis pollen were eluted in theⅠandⅡpeak.The study shows pollen extracts from Amaranthus viridis pollen was purified and characterized and the major allergen was 75 kD a and 67 kD a protein.
基金supported by the National Natural Science Foundation of China(32102605)the Agricultural Science and Technology Innovation Program under Grant(CAAS-ASTIP-2020-IAR)。
文摘Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lactic acid bacteria(LAB)were used to ferment Brassica napus BP for alleviating its allergenicity.Four novel allergens(glutaredoxin,oleosin-B2,catalase and lipase)were identified with significant decreases in LAB-fermented BP(FBP)than natural BP by proteomics.Meanwhile,metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP,indicating the degradation of LAB on allergens.Moreover,FBP showed alleviatory effects in BALB/c mice,which relieved pathological symptoms and lowered production of allergic mediators.Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity,which were closely correlated with the alleviation of allergic reactivity.These findings could contribute to the development and utilization of hypoallergenic BP products.
文摘This study set out to look into the palyno-morphological characteristics of specific allergenic species from diverse plant families in Samarkand, Uzbekistan. Six different species i.e. Betula pendula, Fraxinus pennsylvanica, Magnolia × soulangeana, Pinus brutia var. eldarica, Populus alba, Quercus robur were collected, pressed, identified, and then examined under a microscope. Under light microscope, both quantitative and qualitative characteristics of the pollen grains were recorded, including pollen type, pollen size, pollen shape (polar and equatorial view), P/E ratio, mesocolpium distance, exine thickness, colpi type, colpi length, and width. It was found that a warm spring in Uzbekistan causes an early commencement of the vegetative and dusting phases, in contrast to the climatic circumstances of the researched entities’ motherland (cold spring). Studies in aeropalinology and morphology enable the resolution of numerous environmental issues. The findings highlighted the importance of pollen morphology as an identifying aid and showed how the tested species’ palynological characteristics varied. In order to promote future phylogenetic description of the Uzbekistan flora, the current study may help us better understand the palynomorphological traits of native plants. This essay only briefly and inadequately addresses these issues. The information gathered will be used to make an atlas of allergic plants in Samarkand and an electronic warning system for the general public about the spread of pollen from the city’s allergenic plants in the future.
