The exogenous total DNA of the wild high-protein soybean was transferred to cultivated soybean through the pollen tube channel and the genomic variation of the transformed progeny was detected by the method of RAPD(Ra...The exogenous total DNA of the wild high-protein soybean was transferred to cultivated soybean through the pollen tube channel and the genomic variation of the transformed progeny was detected by the method of RAPD(Random Amplified Polymorphic DNA). Distinguished variations were found in one of the 7 transformed plants of the first generation(D_1),of which the traits of fruition,outward appearance,leaf shape and flower colour were almost identical with those of the recipient parent;of which grain weight,seed coat colour and stem strength were situated between the two parents;and there were greatly more pods per plant and 12.5% higher content of protein in seeds than that of the recipient parent.All the properties have been invariably inherited for 3 generations. Through RAPD analysis of the genomes of the donor,the recipient and the transformed progeny(D_3)as a group,DNA polyrnorphisms were found in amplified products by 24 of 150 primers.The results prove that the exogenous DNA caused the distinct variance of the genome.The authors infer that the homogeneous recombination of large exogenous DNA is the main cause for the variance.展开更多
文摘The exogenous total DNA of the wild high-protein soybean was transferred to cultivated soybean through the pollen tube channel and the genomic variation of the transformed progeny was detected by the method of RAPD(Random Amplified Polymorphic DNA). Distinguished variations were found in one of the 7 transformed plants of the first generation(D_1),of which the traits of fruition,outward appearance,leaf shape and flower colour were almost identical with those of the recipient parent;of which grain weight,seed coat colour and stem strength were situated between the two parents;and there were greatly more pods per plant and 12.5% higher content of protein in seeds than that of the recipient parent.All the properties have been invariably inherited for 3 generations. Through RAPD analysis of the genomes of the donor,the recipient and the transformed progeny(D_3)as a group,DNA polyrnorphisms were found in amplified products by 24 of 150 primers.The results prove that the exogenous DNA caused the distinct variance of the genome.The authors infer that the homogeneous recombination of large exogenous DNA is the main cause for the variance.
