[Objective]The aim of this study was to understand the role of polyamine oxidative degradation in the process of lettuce seed germination. [Method]After lettuce seed soaking treatments with aminoguanidine （AG,a speci...[Objective]The aim of this study was to understand the role of polyamine oxidative degradation in the process of lettuce seed germination. [Method]After lettuce seed soaking treatments with aminoguanidine （AG,a specific inhibitor of polyamine oxidases）,seed germination rate,activities of polyamine oxidase （PAO） and diamine oxidase （DAO）,change of endogenous polyamine and H2O2 content were determined. [Result]Compared with the control,AG treatment strongly inhibited the seed germination,which also had an extremely significant difference in seed germination rate after incubation for 12 h. During the seed germination,activities of PAO and DAO significantly changed,while their activities firstly increased and then decreased,then DAO and PAO reached peaks at 24 h and 48 h respectively. AG treatment was strongly inhibitory for activities of DAO and PAO,whose activities even disappeared after incubation with AG for 24 h and 36 h. During the seed germination,endogenous Put reduced sharply in the first 24 h,then reduced slowly in 24-60 h,while Spd decreased slowly in the early stage of germination,and then sharply declined after 48 h. However,Spm content was low but slightly increased,and the total polyamine gradually decreased. AG treatment could significantly increase endogenous polyamines,especially Put and Spd contents. During the seed germination,H2O2 content gradually increased,and had a peak from 36 to 48 h,then kept a high level at last. AG treatment could significantly reduce H2O2 content. [Conclusion]During the seed germination,the changes of endogenous polyamine and H2O2 content correspond with the changes of PAO and DAO activities,which indicate that there is an active polyamine metabolism of oxidative degradation during the lettuce seed germination.展开更多
Aromatic rice is considered an important commodity in the global market because of its strong aroma and eating and cooking quality.Asian countries,such as India and Pakistan,are the leading traders of Basmati rice,whe...Aromatic rice is considered an important commodity in the global market because of its strong aroma and eating and cooking quality.Asian countries,such as India and Pakistan,are the leading traders of Basmati rice,whereas Thailand is the major supplier of Jasmine rice in the international market.The strong aroma of rice is associated with more than 300 volatile compounds,among which 2-acetyl-1-pyrroline(2-AP)is the principal component.2-AP is a phenotypic expression of spontaneous mutations in the recessive gene OsBadh2 or Badh2.The present review focuses on the origin,evolution and diversity of genetic resources of aromatic rice available worldwide.A brief discussion is presented on the genes responsible for quality traits along with details of their molecular genetics.This compilation and discussion will be useful for future breeding programs and the biofortification of quality traits of aromatic rice to ensure food security and nutritional need.展开更多
基金Supported by Doctorate Fund of Suzhou University (13120740)~~
文摘[Objective]The aim of this study was to understand the role of polyamine oxidative degradation in the process of lettuce seed germination. [Method]After lettuce seed soaking treatments with aminoguanidine （AG,a specific inhibitor of polyamine oxidases）,seed germination rate,activities of polyamine oxidase （PAO） and diamine oxidase （DAO）,change of endogenous polyamine and H2O2 content were determined. [Result]Compared with the control,AG treatment strongly inhibited the seed germination,which also had an extremely significant difference in seed germination rate after incubation for 12 h. During the seed germination,activities of PAO and DAO significantly changed,while their activities firstly increased and then decreased,then DAO and PAO reached peaks at 24 h and 48 h respectively. AG treatment was strongly inhibitory for activities of DAO and PAO,whose activities even disappeared after incubation with AG for 24 h and 36 h. During the seed germination,endogenous Put reduced sharply in the first 24 h,then reduced slowly in 24-60 h,while Spd decreased slowly in the early stage of germination,and then sharply declined after 48 h. However,Spm content was low but slightly increased,and the total polyamine gradually decreased. AG treatment could significantly increase endogenous polyamines,especially Put and Spd contents. During the seed germination,H2O2 content gradually increased,and had a peak from 36 to 48 h,then kept a high level at last. AG treatment could significantly reduce H2O2 content. [Conclusion]During the seed germination,the changes of endogenous polyamine and H2O2 content correspond with the changes of PAO and DAO activities,which indicate that there is an active polyamine metabolism of oxidative degradation during the lettuce seed germination.
基金University Grant Commission, New Delhi, India for providing Non-National Eligibility Test fellowship
文摘Aromatic rice is considered an important commodity in the global market because of its strong aroma and eating and cooking quality.Asian countries,such as India and Pakistan,are the leading traders of Basmati rice,whereas Thailand is the major supplier of Jasmine rice in the international market.The strong aroma of rice is associated with more than 300 volatile compounds,among which 2-acetyl-1-pyrroline(2-AP)is the principal component.2-AP is a phenotypic expression of spontaneous mutations in the recessive gene OsBadh2 or Badh2.The present review focuses on the origin,evolution and diversity of genetic resources of aromatic rice available worldwide.A brief discussion is presented on the genes responsible for quality traits along with details of their molecular genetics.This compilation and discussion will be useful for future breeding programs and the biofortification of quality traits of aromatic rice to ensure food security and nutritional need.
