[Objectives]To explore the effects of Polygona fallax Hemsl water extract on gastrointestinal motility in normal mice and gastric motility disorder model mice and approximate mechanism.[Methods]Using normal mice and m...[Objectives]To explore the effects of Polygona fallax Hemsl water extract on gastrointestinal motility in normal mice and gastric motility disorder model mice and approximate mechanism.[Methods]Using normal mice and mice with gastric motility disorders(modeled with atropine),the effects of different mass concentration groups of P.fallax Hemsl water extract(0.125,0.250,0.500 g/mL)and domperidone groups on gastric residual rate,small intestine propulsion rate,serum motilin(MLT),vasoactive intestinal peptide(VIP),and tissue morphology were studied.[Results]There was a highly significant difference(P<0.01)in the small intestine propulsion rate of liquid in normal mice among the different concentration groups of P.fallax Hemsl water extract compared to the blank group.The small intestine propulsion rate and gastric residue rate of semi-solid paste were statistically significant compared to the blank group(P<0.05).Among them,there was a highly significant difference between the high concentration group(67.75%±7.65%,46.5%±10.62%)and the medium concentration group(60.90%±5.87%,59.27%±7.82%)(P<0.01).There was statistical significance in normal mouse serum MLT content in the high concentration group(P<0.05).There was no effect on serum VIP levels in normal mice;no effect on the morphology of stomach and intestinal tissues of normal mice.The small intestine propulsion rate and gastric residue rate of liquid and semi-solid paste in mice with gastric motility disorders were statistically significant compared to the atropine group,with extremely significant differences(P<0.01).[Conclusions]P.fallax Hemsl water extract has a promoting effect on gastrointestinal motility.One of the specific mechanisms by which P.fallax Hemsl promotes gastrointestinal motility in normal mice may be related to the content of MLT in mouse serum.The mechanism of action in atropine induced gastric paresis mice may be related to the reactivation of M receptors,and the action mechanism of P.fallax Hemsl does not change the original histological basis.It can be inferred that P.fallax Hemsl water extract has a synergistic effect on promoting gastrointestinal motility through other mechanisms,but it is not fully understood and further in-depth research is needed.展开更多
Objective:To study the effects of the ethnic medicine Polygala fallax Hemsl with Guangxi characteristics on the sex hormones andβ-EP in research objective perimenopausal rat models.Methods:40 female SPF rats were ran...Objective:To study the effects of the ethnic medicine Polygala fallax Hemsl with Guangxi characteristics on the sex hormones andβ-EP in research objective perimenopausal rat models.Methods:40 female SPF rats were randomly divided into 4 groups,including the normal,model,high-dose and lowdose groups.Rats of three groups except for the normal one were treated with perimenopausal modelling through the method of subcutaneous injection of compound 4-VCD for 15 consecutive days.Rats of the normal and model group were normally fed without any treatment.Rats of the high-dose and low-dose groups were administered by high-and low-dose intragastric administration of the extract of Polygala fallax Hemsl.According to the menstrual cycle of the vaginal smear of the rat,each menstrual cycle is a course of treatment and 6 consecutive courses of treatment would be given.The indexes of serum sex hormones(E2,FSH,LH)andβ-EP of rats in each group were observed after treatment.Results:After the treatment of 6 cycles,for the levels ofβ-EP and E2,the model group was lowest(P<0.05),the normal group was highest(P<0.05);and the high-dose group was higher than the low-dose group;For the levels of FSH and LH,the normal group was lowest(P<0.05),the model group was highest(P<0.05),and the high-dose group was lower than the low-dose group.Conclusion:Guangxi characteristic national medicine Polygala fallax Hemsl can effectively improve the levels of serum sex hormones andβ-EP in perimenopausal rat models and relieve the related symptoms with a certain dose-effect relationship.展开更多
[Objectives]The paper was to study the effects of polysaccharide from Polygala fallax on apoptosis of B16 melanoma and its mechanism.[Methods]P.fallax polysaccharide was prepared by water extraction and alcohol precip...[Objectives]The paper was to study the effects of polysaccharide from Polygala fallax on apoptosis of B16 melanoma and its mechanism.[Methods]P.fallax polysaccharide was prepared by water extraction and alcohol precipitation method combined with Sevag method.The effects of different concentrations of P.fallax polysaccharide on B16 cell proliferation were detected by MTT assay.Apoptosis was detected by Hoechst 33258 staining assay and flow cytometry.The expressions of Bcl-2,Bax,Cleaved Caspase-3 mRNA were detected by Real-time PCR assay.The expressions of Bcl-2,Bax,Caspase-3,Caspase-8,Caspase-9,Cleaved Caspase-3,Cleaved Caspase-9,ERK,p-ERK,JNK,p-JNK proteins were detected by Western Blot assay.[Results]P.fallax polysaccharide inhibited the cell proliferation of B16 melanoma in a concentration-dependent manner(24 h IC 50=0.2133 mg/L;48 h IC 50=0.08489μg/mL).P.fallax polysaccharide up-regulated the expressions of Bax,Cleaved Caspase-3 mRNA and protein,down-regulated the expressions of Bcl-2 mRNA and protein,activated Caspase-3,Caspase-8 and Caspase-9,and decreased the levels of p-ERK/ERK and p-JNK/JNK.[Conclusions]P.fallax polysaccharide can inhibit the cell proliferation of B16 melanoma and induce their apoptosis,probably attributed to the regulation of MAPK,Bcl-2 and Caspase family signaling pathways.展开更多
基金2022 National College Student Innovation and Entrepreneurship Training Program(202210599004).
文摘[Objectives]To explore the effects of Polygona fallax Hemsl water extract on gastrointestinal motility in normal mice and gastric motility disorder model mice and approximate mechanism.[Methods]Using normal mice and mice with gastric motility disorders(modeled with atropine),the effects of different mass concentration groups of P.fallax Hemsl water extract(0.125,0.250,0.500 g/mL)and domperidone groups on gastric residual rate,small intestine propulsion rate,serum motilin(MLT),vasoactive intestinal peptide(VIP),and tissue morphology were studied.[Results]There was a highly significant difference(P<0.01)in the small intestine propulsion rate of liquid in normal mice among the different concentration groups of P.fallax Hemsl water extract compared to the blank group.The small intestine propulsion rate and gastric residue rate of semi-solid paste were statistically significant compared to the blank group(P<0.05).Among them,there was a highly significant difference between the high concentration group(67.75%±7.65%,46.5%±10.62%)and the medium concentration group(60.90%±5.87%,59.27%±7.82%)(P<0.01).There was statistical significance in normal mouse serum MLT content in the high concentration group(P<0.05).There was no effect on serum VIP levels in normal mice;no effect on the morphology of stomach and intestinal tissues of normal mice.The small intestine propulsion rate and gastric residue rate of liquid and semi-solid paste in mice with gastric motility disorders were statistically significant compared to the atropine group,with extremely significant differences(P<0.01).[Conclusions]P.fallax Hemsl water extract has a promoting effect on gastrointestinal motility.One of the specific mechanisms by which P.fallax Hemsl promotes gastrointestinal motility in normal mice may be related to the content of MLT in mouse serum.The mechanism of action in atropine induced gastric paresis mice may be related to the reactivation of M receptors,and the action mechanism of P.fallax Hemsl does not change the original histological basis.It can be inferred that P.fallax Hemsl water extract has a synergistic effect on promoting gastrointestinal motility through other mechanisms,but it is not fully understood and further in-depth research is needed.
基金Project for Improving Basic Capabilities of Middle-aged and Young Teachers in Guangxi Institutions of Higher Learning(No.KY2016YB835)National Natural Science Foundation of China(No.81660830)+3 种基金Open Project for Guangxi Key Laboratory of Chinese Medicine Foundation Research of Guangxi(No.15-140-32-06)Open Project for Guangxi First-class Discipline Construction of Guangxi University of Chinese Medicine(No.2019XK038)Funded by Development Program of High-level Talent Team under Qihuang Project of Guangxi University of Chinese Medicine(No.2018005)Traditional Chinese Medicine-Guangxi first-class discipline construction project(No.Gui Jiao Ke Yan[2018]12).
文摘Objective:To study the effects of the ethnic medicine Polygala fallax Hemsl with Guangxi characteristics on the sex hormones andβ-EP in research objective perimenopausal rat models.Methods:40 female SPF rats were randomly divided into 4 groups,including the normal,model,high-dose and lowdose groups.Rats of three groups except for the normal one were treated with perimenopausal modelling through the method of subcutaneous injection of compound 4-VCD for 15 consecutive days.Rats of the normal and model group were normally fed without any treatment.Rats of the high-dose and low-dose groups were administered by high-and low-dose intragastric administration of the extract of Polygala fallax Hemsl.According to the menstrual cycle of the vaginal smear of the rat,each menstrual cycle is a course of treatment and 6 consecutive courses of treatment would be given.The indexes of serum sex hormones(E2,FSH,LH)andβ-EP of rats in each group were observed after treatment.Results:After the treatment of 6 cycles,for the levels ofβ-EP and E2,the model group was lowest(P<0.05),the normal group was highest(P<0.05);and the high-dose group was higher than the low-dose group;For the levels of FSH and LH,the normal group was lowest(P<0.05),the model group was highest(P<0.05),and the high-dose group was lower than the low-dose group.Conclusion:Guangxi characteristic national medicine Polygala fallax Hemsl can effectively improve the levels of serum sex hormones andβ-EP in perimenopausal rat models and relieve the related symptoms with a certain dose-effect relationship.
基金Supported by Scientific Research and Technology Development Program of Guilin City(2020011203-1)Guilin Science and Technology Development Program(20210202-1)+1 种基金the Open Funds of the Guangxi Key Laboratory of Tumor Immunology and Microenvironmental Regulation(2022KF005)Guangxi Major Science and Technology Project(GK AA22096020).
文摘[Objectives]The paper was to study the effects of polysaccharide from Polygala fallax on apoptosis of B16 melanoma and its mechanism.[Methods]P.fallax polysaccharide was prepared by water extraction and alcohol precipitation method combined with Sevag method.The effects of different concentrations of P.fallax polysaccharide on B16 cell proliferation were detected by MTT assay.Apoptosis was detected by Hoechst 33258 staining assay and flow cytometry.The expressions of Bcl-2,Bax,Cleaved Caspase-3 mRNA were detected by Real-time PCR assay.The expressions of Bcl-2,Bax,Caspase-3,Caspase-8,Caspase-9,Cleaved Caspase-3,Cleaved Caspase-9,ERK,p-ERK,JNK,p-JNK proteins were detected by Western Blot assay.[Results]P.fallax polysaccharide inhibited the cell proliferation of B16 melanoma in a concentration-dependent manner(24 h IC 50=0.2133 mg/L;48 h IC 50=0.08489μg/mL).P.fallax polysaccharide up-regulated the expressions of Bax,Cleaved Caspase-3 mRNA and protein,down-regulated the expressions of Bcl-2 mRNA and protein,activated Caspase-3,Caspase-8 and Caspase-9,and decreased the levels of p-ERK/ERK and p-JNK/JNK.[Conclusions]P.fallax polysaccharide can inhibit the cell proliferation of B16 melanoma and induce their apoptosis,probably attributed to the regulation of MAPK,Bcl-2 and Caspase family signaling pathways.
