Optimization of Extraction Technology of Auricularia auricula Polysaccharide

[Objective]This experiment aimed to study the extraction technology of Auricularia auricula polysaccharide（APS）.[Method] Polysaccharide yield was regarded as studying target.Based on the single factor experiment results,orthogonal experiment design was applied to study the effect of liquid-solid ratio,extraction time,extraction temperature on polysaccharide yield.[Result]The results showed that extraction temperature was an important factor that affects the yield of crude polysaccharide,the optimal technology condition of water extraction way was liquid-solid ratio of 1：70（g：ml）,4-hour extraction time with temperature at 100 ℃.[Conclusion] The yield of Auricularia auricular polysaccharide was 42.56% under the above mentioned condition.

Optimization of Ultrasonic-Assisted Extraction Process of Polysaccharides from American Ginseng and Evaluation of Its Immunostimulating Activity

Ultrasonic-assisted extraction （UAE） of American ginseng polysaccharides （AGP） was investigated using response surface methodology. Three-factor-three-level Box-Behnken design was employed to optimize the ultrasonic power, extraction time and ratio of water to raw material to obtain a high AGP yield. The analysis of variance and response surface plots indicated that ultrasonic power was the most important factor affecting the extraction yield. The optimal conditions were ultrasonic power 400 W, extraction time 71 min, and ratio of water to raw material 33 mL g-1. Under these conditions, the yield of AGP was 8.09%, which was agreed closely to the predicted value. Gas chromatography （GC） analysis showed that AGP was composed of arabinose, rhamnose, galactose, glucose, and galacturonic acid. Fourier transform infrared spectra revealed the general characteristic absorption peaks of AGP. In addition, AGP exhibited good immunostimulating activities by up-regulating the production of nitric oxide and cytokines. Compared with hot water extraction, UAE required shorter extraction time and gave a higher extraction yield, without changing the structure and immunostimulating activity of AGP. The results indicated that UAE could be an effective and advisable technique for the large scale production of plant polysaccharides.

Review on the Research Progress of Dictyophora rubrovolvata

Dictyophora rubrovolvata has beautiful shape,good taste and high nutritional value,and has anti-oxidation,anti-tumor,hypoglycemic and other effects.In this review,the biological characteristics,nutritive chemical composition,polysaccharide extraction and functions,cultivation and pest control of D.rubrovolvata were intensively discussed and summarized,and the future research direction was also prospected.This study aims to provide future recommendations for the research of D.rubrovolvata and then promote the development of D.rubrovolvata industry.