This study was carried out to determine the effect of 21 Citrus interstocks and the control Citrus macrophylla rootstock budded with Mexican lime (Citrus aurantifolia (Chistm.) Swingle) on growth and yield. Three ...This study was carried out to determine the effect of 21 Citrus interstocks and the control Citrus macrophylla rootstock budded with Mexican lime (Citrus aurantifolia (Chistm.) Swingle) on growth and yield. Three types of mandarin and 13 trifoliate types including "C-35" and "Benton" used as interstocks, developed vigorous trees similar or more productive than the control. "Morton" and "Benecke" citranges, "Pomeroy" trifoliate trifoliate orange and the "Severinia buxifolia" interstocks showed a moderated effect on tree growth. Flying dragon (FDT) and Hiryu trifoliate oranges performed as semi-dwarfing interstocks. These interstocks reduced the tree height, canopy volume and surface area covered by the foliage on 25%-35%, 20%-30% and 20%-30%, respectively, compared to the control. While most interstocks surpassed the assigned area of growth (24 m2) in the 7th year, "FDT" and "Hiryu" did that at the 10th year. These semi-dwarfing interstocks produced up to 80 kg/tree per year. Most treatments recorded the highest production from the 8th to 10th year, except FDT and Hiryu, which showed the highest yield (130 kg/tree) from the 5th to 7th year. This study provides useful results to face the huanglongbing (HLB) problem using moderate or semi-dwarfing interstocks for Mexican lime orchards in high density plantations.展开更多
Forsythia fructus has been shown to have antioxidative, anti-inflammatory, antibacterial, anti-aging and whitening effects. Hoechunyangkyeok-san (Forsythia viridissima-prescription) is a traditional herbal medicine, w...Forsythia fructus has been shown to have antioxidative, anti-inflammatory, antibacterial, anti-aging and whitening effects. Hoechunyangkyeok-san (Forsythia viridissima-prescription) is a traditional herbal medicine, which has been clinically used for treating febrile and inflammatory disorders. This work was carried out to investigate the skin whitening effects of Forsythia viridissima-prescription extract (a hydrolyzed extract of Hoechunyangkyeok-san: SID White HYC) on skin. The effects of SID White HYC were assessed the melanin contents in B161 melanoma cells and the pigmented equivalent with HMB45 and Fontana Masson staining in 3D skin model. Then, we examined the expression of major pigment enzymes regulating melanin synthesis and melanosome transport related proteins in B16F1 cells. SID White HYC significantly inhibited the melanin synthesis (56.7% and 30.6% inhibition at 100 μg/mL, intracellular and secreted, respectively) in B16F1 cells and 3D skin model. In addition, western blotting analysis showed that SID White HYC reduced the expression of melanin synthesis and melanosome transport related proteins in B16F1 cells. In clinical trials, the cream containing 0.05% SID White HYC showed skin depigmentation effect without any irritation. These results suggest that SID White HYC may be useful inhibition of melanogenesis and melanosome transport. Therefore, SID White HYC may have potential as a skin-whitening ingredient in cosmetics.展开更多
文摘This study was carried out to determine the effect of 21 Citrus interstocks and the control Citrus macrophylla rootstock budded with Mexican lime (Citrus aurantifolia (Chistm.) Swingle) on growth and yield. Three types of mandarin and 13 trifoliate types including "C-35" and "Benton" used as interstocks, developed vigorous trees similar or more productive than the control. "Morton" and "Benecke" citranges, "Pomeroy" trifoliate trifoliate orange and the "Severinia buxifolia" interstocks showed a moderated effect on tree growth. Flying dragon (FDT) and Hiryu trifoliate oranges performed as semi-dwarfing interstocks. These interstocks reduced the tree height, canopy volume and surface area covered by the foliage on 25%-35%, 20%-30% and 20%-30%, respectively, compared to the control. While most interstocks surpassed the assigned area of growth (24 m2) in the 7th year, "FDT" and "Hiryu" did that at the 10th year. These semi-dwarfing interstocks produced up to 80 kg/tree per year. Most treatments recorded the highest production from the 8th to 10th year, except FDT and Hiryu, which showed the highest yield (130 kg/tree) from the 5th to 7th year. This study provides useful results to face the huanglongbing (HLB) problem using moderate or semi-dwarfing interstocks for Mexican lime orchards in high density plantations.
文摘Forsythia fructus has been shown to have antioxidative, anti-inflammatory, antibacterial, anti-aging and whitening effects. Hoechunyangkyeok-san (Forsythia viridissima-prescription) is a traditional herbal medicine, which has been clinically used for treating febrile and inflammatory disorders. This work was carried out to investigate the skin whitening effects of Forsythia viridissima-prescription extract (a hydrolyzed extract of Hoechunyangkyeok-san: SID White HYC) on skin. The effects of SID White HYC were assessed the melanin contents in B161 melanoma cells and the pigmented equivalent with HMB45 and Fontana Masson staining in 3D skin model. Then, we examined the expression of major pigment enzymes regulating melanin synthesis and melanosome transport related proteins in B16F1 cells. SID White HYC significantly inhibited the melanin synthesis (56.7% and 30.6% inhibition at 100 μg/mL, intracellular and secreted, respectively) in B16F1 cells and 3D skin model. In addition, western blotting analysis showed that SID White HYC reduced the expression of melanin synthesis and melanosome transport related proteins in B16F1 cells. In clinical trials, the cream containing 0.05% SID White HYC showed skin depigmentation effect without any irritation. These results suggest that SID White HYC may be useful inhibition of melanogenesis and melanosome transport. Therefore, SID White HYC may have potential as a skin-whitening ingredient in cosmetics.