Extraction of Total DNA from Populus euphratica Oliv. and Populus pruinosa Schrenk. by Improved CTAB Method

This study presented an improved CTAB method for extracting DNA from leaves of Populus euphratica Oliv. and Populus pruinosa Schrenk. based on the conventional CTAB method. The results showed that preventing DNA from browning is a key step to obtain the high-quality DNA during DNA extraction, and under the condition of grinding in the presence of liquid nitrogen, adding such three antioxidants as PVP dry powder, Vc and β-mercaptoethanol could prevent DNA from browning effectively. The total DNA extracted by the improved CTAB method was subjected to PCR detection which proved that it totally satisfied the requirements of subsequent study.

Evapotranspiration of a Populus euphratica Oliv. forest and its controlling factors in the lower Heihe River Basin,Northwest China

Evapotranspiration (ET) within an ecosystem is crucial for die water-limited environment that currently lacks adequate quantification in the arid region of Northwest China, mainly covered by phreatophytes, such as the Populus euphratica Oliv. tree and the Tamarix ramosissima Ledeb. shrub species. Accordingly. ET was measured for an entire year using eddy covariance (EC) in P. euphratica stands in the lower Heihe River Basin, Northwest China. During the growing season, the total ET was 850 mm, with a mean of 4.0 mm/d, Which is obviously more than that observed at tree-level and stand level scales, which was likely due to the different level of soil evaporation induced by irrigation via water conveyance. Factors associated with ET fall into either environmental or plant eco-physiological categories. Environmental factors account for at least 79% variation of ET and the linear relationship between ET and the voundwater table (GWT) revealed the potential water use of P. euphratica forests under the non-water stress condition with die GWT less than 3 m deep. Plant eco-physiological parameters, specifically die leaf area 'index (LAI), have direct impact on the seasonal pattern of ET which provides a valuable reference to the wide-area estimates of ET for riparian forests by using LAI. In conclusion, P. euphratica forests have high water use after water conveyance, which may be the result of long-term adapting to local climates and limited water availability.

胡杨(Populus euphratica Oliv.)根繁殖特征

以新疆塔里木河中游河岸北部河漫滩的胡杨幼林带为对象,从克隆生态学的角度,采用挖掘法、高压冲洗法及断根胁迫实验,研究了胡杨在资源异质环境中的风险分担、资源觅食与保持功能以及根源性克隆生长形态特征。结果表明:胡杨的地下根分枝类型为单轴型,同一生长方向2水平根的夹角平均约为180°,水平根与垂直根的夹角为90°～100°,水平根和垂直根的空间构型近似T形;基株的胚根周长>分株的次根周长;水平根呈侧圆台形,胡杨的2水平根之间存在着养分的传递。

Cloning of plasma membrane H^+-ATPase gene in Populus euphratica Oliv.

For this paper, the plasma membrane （PM） H^＋-ATPase gene has been cloned from Populus euphratica Oliv. through a ho- mology based strategy. The isolated 3,210 bp cDNA contains a single 2,862 bp open reading frame （ORF） which encodes a putative H^＋-ATPase protein of 953 amino acid residues, with a significant homology to plasma membrane H^＋-ATPase of Prunus persica, Phaseolus vulgaris, Sesbania rostrata and Daucus carota. The predicted protein has a molecular weight of 104,553 Da. The copy number analysis revealed multiple copies of the PM H^＋-ATPase in the P. euphratica genome after digestion of their genomic DNA by the restriction enzymes EcoRI, NdeI, FbaI and Bg/Ⅱ, and Southern blot.

Electrophoresis Analysis on the Protein Expression in Heteromorphic Leaves of Populus euphratica Oliv.

[Objective] This study was to elucidate the cellular and molecular mechanism of the development of heteromorphic leaves of Populus euphratica Oliv. [Method] By employing SDS-PAGE and 2-demensional electrophoresis (2-DE) techniques,proteins in various heteromorphic leaves from the same adult tree of P. euphratica were isolated and separated to the electrophoresis technique suitable for the separation and analysis of proteins in leaves of P. euphratica tree. [Results] There were significant differences in the expressions of proteins in various heteromorphic leaves of P. euphratica tree. SDS-PAGE pattern showed that bands of proteins with molecular weight of 57.2,13.2,30.2,23.9 and 33.3 kDa were remarkably different. 2-D electrophoresis pattern presented that proteins in leaves of P. euphratica tree mainly belong to acidic proteins distributed at pH value of 5.0-6.5 and with molecular weight of 20-40 kDa; totally 73 different protein spots were observed,of which 51 were up expressed and other 22 were down expressed in the serrated ovate leaves. [Conclusion] Based on these results,we speculate that regulated gene expression in leaves of P. euphratica tree results in the generation of different shapes of leaves,in order to adapt to the surroundings better.

Analysis of the Correlation between the Geographical Distribution of Populus euphratica Oliv. and the Climatic Environment

[Objective] This research was to study the correlation regional climatic characteristics and changing geographic distribution of Populus euphratica Oliv. (Salicaceae), as well as the adaption of Populus euphratica Oliv. to the climatic environment. [Method] The climatic characteristics, water source, groundwater and soil type in the distribution regions of Populus euphratica Oliv. and the effect of long-term human activities were comprehensively analyzed based an overview of Populus euphratica Oliv. and its distribution. [Result] Specific regional climatic characteristics and long term human activities are the principle determinants for the growth of Populus euphratica Oliv. The change of leaf shape is a distinct feature of Populus euphratica Oliv. in adapting to the climatic environment. Populus euphratica Oliv. withstands various environmental stresses by means of in vivo synthesis, transport and conversion of secondary phenolic metabolites. Effective protection and rehabilitation measures, and ecological water transport have obvious effect on the restoration and reconstruction of damaged ecological environment of Populus euphratica oasis. [Conclusion] This study is of great significance for the restoration of ecological environment in the arid inland regions, north-west China.

Effects of temperature on flowering phenological traits of Populus euphratica Oliv. and Populus pruinosa Schrenk populations, Xinjiang, China

The aims of this study were to explore the interspecific differences of Populus euphratica Oliv.and Populus pruinosa Schrenk populations and the intraspecific differences of males and females within the same species in flowering phenological traits,and the effects of temperatures on flowering phenological traits in different growth years(2001–2003 and 2013–2015).The results showed that P.euphratica population flowered earlier than P.pruinosa population.Moreover,flowering phenological period of population,number of days of flowering phenological period per population,number of days of flowering phenological period per plant and average number of days of flowering period per plant of P.euphratica population were less than those of P.pruinosa population.The differences between male and female within the same species indicated that the flowering periods of males P.euphratica and P.pruinosa populations were earlier than those of female plants.For both species,flowering phenological traits were significantly and negatively correlated with the average temperatures in previous ten days,previous one month and previous three months of flowering.Both species are sensitive to temperature changes and adjust to the changes by advancing the start of flowering and prolonging the duration of flowering.

Impacts of Defoliation on Morphological Characteristics and Non-Structural Carbohydrates of Populus talassica × Populus euphratica Seedlings

Leaves are important‘source’organs that synthesize organic matter,providing carbon sources for plant growth.Here,we used Populus talassica×Populus euphratica,the dominant species in ecological and timber forests,to simulate carbon limitation through artificial 25%,50%,and 75%defoliation treatments and explore the effects on root,stem,and leaf morphology,biomass accumulation,and carbon allocation strategies.At the 60th d after treat-ment,under 25%defoliation treatment,the plant height,specific leaf weight,root surface area and volume,and concentrations of non-structural carbohydrates in stem and root were significantly increased by 9.13%,20.00%,16.60%,31.95%,5.12%,and 9.34%,respectively,relative to the control.There was no significant change in the growth indicators under 50%defoliation treatment,but the concentrations of non-structural carbohydrates in the leaf and stem significantly decreased,showing mostly a negative correlation between them.The opposite was observed in the root.Under 75%defoliation treatment,the plant height,ground diameter,leaf number,single leaf area,root,stem,and total biomass were significantly reduced by 14.15%,10.24%,14.86%,11.31%,11.56%,21.87%,and 16.82%,respectively,relative to the control.The concentrations of non-structural carbohydrates in various organs were significantly reduced,particularly in the consumption of the starch concentrations in the stem and root.These results indicated that carbon allocation strategies can be adjusted to increase the con-centration of non-structural carbohydrates in root and meet plant growth needs under 25%and 50%defoliation.However,75%defoliation significantly limited the distribution of non-structural carbohydrates to roots and stems,reduced carbon storage,and thus inhibited plant growth.Defoliation-induced carbon limitation altered the carbon allocation pattern of P.talassica×P.euphratica,and the relationship between carbon reserves in roots and tree growth recovery after defoliation was greater.This study provides a theoretical basis for the comprehen-sive management of P.talassica×P.euphratica plantations,as well as a reference for the study of plantation car-bon allocation strategies in the desert and semi-desert regions of Xinjiang under carbon-limitation conditions.

Trunk volume estimation of irregular shaped Populus euphratica riparian forest using TLS point cloud data and multivariate prediction models

Background: Trunk volume(Vt) is an essential parameter for estimating forest stand volume, biomass, and carbon sequestration potential. As the dominant tree species in desert riparian forests, Euphrates poplar(Populus euphratica) has a high proportion of irregularly shaped tree trunks along the Tarim River, NW China, where the habitat is very fragile owing to long-term water stress. This causes uncertainty in estimation accuracy as well as technical challenges for forest surveys. Our study aimed to acquire P. euphratica Vtusing terrestrial laser scanning(TLS) and to establish a species-specific Vtprediction model.Methods: A total of 240 individual trees were measured by TLS multiple-station in 12 sampling plots in three sections along the lower reaches of the Tarim River. Vtwas calculated by a definite integration method using trunk diameters(Di) at every 0.1-m tree height obtained from TLS, and all data were split randomly into two sets:70% of data were used to estimate the model parameter calibration, and the remaining 30% were used for model validation. Sixteen widely used candidate tree Vtestimation models were fitted to the TLS-measured Vtand tree structural parameter data, including tree height(H), diameter at breast height(DBH), and basal diameter(BD). All model performances were evaluated and compared by the statistical parameters of determination coefficient(R^(2)),root mean square error(RMSE), Bayesian information criterion(BIC), mean prediction error(ME), mean absolute error(MAE), and modeling efficiency(EF), and accordingly the best model was selected.Results: TLS point cloud reflection intensity(RI) has advantageous in the extraction of data from irregular tree trunk structures. The P. euphratica tree Vtvalues showed obvious differences at the same tree height(H). There was no significant correlation between Vtand H(R^(2)=0.11, P < 0.01), which reflected the irregularity of P. euphratica trunk shape in the study area. Among all the models, model(14): Vt=0.909DBH1.184H0.487BD0.836(R^(2)=0.97, RMSE=0.14) had the best prediction capability for irregularly shaped Vtwith the highest R^(2), BIC(-37.96), and EF(0.96), and produced a smaller ME(0.006) and MAE(1.177) compared to other models. The prediction accuracy was 93.18%.Conclusions: TLS point cloud RI has a potential for nondestructively measuring irregularly shaped trunk structures of P. euphratica and developed Vtprediction models. The multivariate models more effectively predicted Vtfor irregularly shaped trees compared to one-way and general volume models.

Analysis on the change of water potential of Populus euphratica Oliv.and P.Russkii Jabl under different irrigation volumes in temperate desert zone

The measurement of plant water potential is considered to be a direct approach to confirming the irrigation time in present irrigation systems.In this current study,two shelter forests species in the Junggar Basin (Central Asia),Populus euphratica and P.Russkii Jabl,were studied.We monitored leaf water potential (ψl),stem water potential (ψs) and soil water potential (ψsoil) under different irrigation conditions.The characteristics of the variation in leaf and stem water potential (ψl and ψs) of P.euphratica and P.Russkii Jabl,as well as the impact of soil water potential (ψsoil) on the leaf and stem water potential (ψl and ψs) under different irrigation conditions were discussed.Our results showed the following.(1) With increased irrigation,the intensity of drought stress on P.euphratica and P.Russkii Jabl decreased.(2) The intensity of drought stress experienced was less for P.Russkii Jabl than for P.euphratica under the same irrigation conditions.(3) The more intense the drought,the less sensitive was P.Russkii Jabl,but the more sensitive was P.euphratica,and vice versa.(4) For the P.euphratica community the soil water potential (Ψsoil) at 60 cm depth responded to variation in irrigation more strongly than at 30 and 90 cm depths.For the P.Russkii Jabl community the soil water potential (ψsoil) in the shallow surface layer responded to irrigation variation more strongly than that in deep layers.(5) In the event of relatively sufficient soil water,predawn stem water potential (ψpds) of plant was a reasonable indicator reflecting soil water potential (ψsoil).(6) The water demand of P.euphratica and P.Russkii Jabl shelter forests can be met with different irrigation policies:large volume and less frequency for P.euphratica but small volume and more frequency for P.Russkii Jabl.

Establishment of in vitro culture of Populus euphratica Olivier

The purpose of our study was to establish a regeneration system for micropropagation of Populus euphratica Olivier. On the basis of an analysis of plant leaf mineral nutrients, a special medium was proposed, called MP2. In optimizing media for in vitro plant cultures including MS, B5 and MP2 media we employed hormones, auxin IAA, cytokine benzyladenine （BAP） and gibberellic acid （GA） in our factorial experiments on media. Adventitious shoots were derived from cuttings of adult plants taken from Xingii- ang, west China, on selected media with MP2^＋ 0.5 mg·L^-1BA＋0.1 mg·L^-1 NAA. The shoots were elongated on a medium with 0.25 mg·L^-1 BAP, 0.1 mg·L^-1NAA and 2 mg·L^-1 GA and were then rooted on a medium with 0.2-0.5 mg·L^-1 IBA. All the media were incorporated with 30 g·L^-1 sucrose and an adjusted pH at 6.3.

胡杨(Populus euphratica)在额济纳绿洲三种生境内的根蘖繁殖特性

以额济纳荒漠绿洲胡杨根蘖幼苗为研究对象,通过对绿洲内胡杨分布的3种主要生境内根蘖幼苗的调查,得出如下结论。近年来绿洲内胡杨种群的更新几乎完全依赖于无性繁殖更新,种子萌发产生的幼苗在调查地内没有发现。河岸沙丘地及胡杨林下地的根蘖幼苗中,3龄幼苗所占比重最大,龄级越小的根蘖苗在群落中的数量越少;在河水漫灌后的林间空地,2龄幼苗的数量最多。水漫灌后的林间空地中根蘖幼苗密度显著大于其余两生境内根蘖幼苗密度,同时该生境内每段胡杨根系萌生出的不定芽也大于另两生境。随着龄级增加,未枯枝根蘖幼苗的比率逐渐减小。根蘖幼苗的萌发点分布于土层30cm以内的根系上,分布深度大于30cm的根系不能萌发出根蘖幼苗。

Isolation of Protoplast and Ion Channel Recording in Plasma Membrane of Suspension Cells of Populus euphratica

The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “onozuka” R\|10, 0\^01% pectolyase Y\|23,0\^15% macerozyme R\|10 and 0\^1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell\|attached recording of patch\|clamp technique. In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species.

Gas Exchange, Xylem Ions and Abscisic Acid Response to Na^+-Salts and Cl^--Salts in Populus euphratica

We investigated the osmotic stress and ion-specific effects on xylem abscisic acid (ABA), ion uptake and transport and gas exchange in one-year-old seedlings of Populus euphratica Oliv. Net photosynthetic rates (P-n) and unit transpiration rates (TRN) were both significantly decreased upon an osmotic shock caused by PEG 6000 solution (osmotic potential = -0.24 MPa) or a saline, which was applied by 50 mmol/L Na+-salts (NaNO3 : NaHCO3 : NaH2PO4 = 5 : 4 : 1, pH 6.8, osmotic potential = -0.24 MPa) or by 50 mmol/L Cl--salts (KCl : NH4Cl = 1:1, osmotic potential = -0.24 MPa). However, salt-treated P. euphratica plants maintained typically higher TRN than those exposed to PEG. Xylem ABA concentrations increased rapidly following the PEG treatment, exhibiting peaking values at 1 h, then returning to pre-stress levels, followed by a gradual increase. Similarly, both Na+-treated and Cl--treated trees exhibited a rapid rise of ABA after salt stress was initiated. Notably, salt-treated plants maintained a relatively higher ABA than PEG-treated plants in a longer term. Collectively, results suggest that osmotic stress and ion-specific effects were both responsible for salt-induced ABA in P. euphratica : the initial rapid increase of xylem ABA appears to be a consequence of an osmotic shock, whereas specific salt effects seem to be responsible for ABA accumulation later on. Compared with Cl--treated trees, a higher inhibitory effect on gas exchange (P-n and TRN) was observed in Na+-salt plants, resulting from its long-sustained ABA and higher salt concentrations in the xylem. Displacement of membrane-associated Ca2+ by Na+ and the lesser capacity in Na+ compartmentation in root vacuoles likely contribute to the high influx of Na+ and Cl- in Na+-treated plants. Xylem K+, Ca2+ and Mg2+ concentrations were elevated by external Na+ -salts and Cl--salts, suggesting that P. euphratica maintained a higher capacity in nutrient uptake under saline conditions, which makes a contribution to its salinity tolerance.

NaCl胁迫下胡杨(Populus euphratica)和群众杨(P.popularis)抗氧化能力及耐盐性

在盐浓度逐渐提高的胁迫条件下，对抗盐的胡杨（Populuseuphratica）和盐敏感的群众杨（Ppopularis35-44）1年实生苗木叶片中Na^＋、Cl^-水平、O2^-产生速率以及抗氧化酶：超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）、过氧化氢酶（CAT）和谷胱甘肽还原酶（GR）的动态变化以及抗盐性进行了研究。结果表明，在盐浓度不断提高的胁迫条件下，群众杨叶片中Na^＋、Cl^-浓度持续增加，盐胁迫18d时，群众杨叶中Na^＋、Cl^-含量分别达到对照的17．8和14．6倍，此时O2^-产生速率也达到最高水平。而在盐胁迫期间，群众杨叶片SOD活性没有明显提高，CAT活性维持在对照水平以下，只有APX和GR活性在盐胁迫13—18d，即盐害症状出现前才有所上升，属于典型的盐害反应。胡杨与群众杨明显不同：在盐胁迫初期，胡杨叶片Na^＋、Cl^-含量虽然没有明显的变化，但胡杨叶片中O2^-产生速率在盐胁7d时明显提高，SOD、APX、CAT活性也都先后相应上升，表明胡杨能响应盐胁迫并上调SOD、APX和CAT等保护酶类，降低盐诱导的膜脂过氧化，从而减少了电解质外渗，最终提高了树木的抗盐性。

额济纳绿洲胡杨(Populus euphratica)酚类物质含量和分布及其与土壤水分的关系

采用分光光度法测定了额济纳绿洲胡杨（Populus euphratica）的披针形叶、卵圆形叶、嫩枝、枝（D〈5mm）、枝（5～10mm）、主干树皮、根（D〈2mm）、根（2～5mm）和根（5～10mm）9类器官中的总酚、黄酮和缩合单宁含量。结果表明，总酚含量较高的器官为皮（27．93mg／g），叶、根、枝中总酚含量分别为17．64mg／g（两类叶均值）、16．72mg／g（三类根均值）、12．19mg／g（三类枝均值）；黄酮含量较高的器官为皮（51．30mg／g），叶、根、枝中黄酮含量分别为28．45mg／g（两类叶均值）、39．99mg／g（三类根均值）、23．67mg／g（三类枝均值）；根中缩合单宁含量较高，三类根均值为22．10mg／g，皮、叶、枝中缩合单宁含量分别为8．41mg／g、4．03mg／g（两类叶均值）、4．47mg／g（三类枝均值）。披针形叶和卵圆形叶中酚类物质含量没有显著性差异（P〉0．05）；随着枝不断成熟，嫩枝、枝（D〈5mm）、枝（5～10mm）中酚类物质逐渐减少；随着根直径减少，根中缩合单宁逐渐增加，细根（D〈2mm）中的缩合单宁含量最高（25．95mg／g）。分析胡杨各器官中酚类物质含量与土壤水分的关系，结果表明卵圆形叶中酚类物质含量与土壤水分含量成显著负相关关系（P〈0．05，总酚：r=-0．949；黄酮：r=-0．923；缩合单宁：r=-0．944）。研究揭示了极端干旱地区胡杨各器官中酚类物质的变化规律，及其与环境因子的相互作用关系。

胡杨(Populus euphratica)径向生长日变化特征分析

利用树木径向生长测定仪、自动气象站和土壤水分自动监测仪,在黑河下游胡杨河岸林内,同步监测了胡杨径向生长日变化和太阳辐射、气温、空气相对湿度及主要根系层土壤水分动态等环境因子.结果表明:胡杨径向生长日变化呈抛物线形,每日16:00～20:00时达到最低值点,随后逐步上升至翌日06:00～09:00时达到最高值点,完成一个由收缩期、膨胀恢复期和生长期3阶段构成的日变化周期;不同季节,胡杨径向生长变动幅度存在较大差异.就监测样树而言,不同胡杨个体,胸径越大,日净生长量越大.对各环境因子统计表明,胡杨径向生长日变化与总辐射、气温和土壤体积含水率呈负相关关系,与空气相对湿度呈正相关关系.各环境因子对胡杨径向生长影响存在延时滞后作用,特别是土壤水分条件.

Studies on the Proton Pumping Activity of H^+-ATPase in Tonoplast Vesicles of Populus euphratica

Tonoplast-enriched vesicles were prepared from suspension-cultured Populus euphratica Oliv. cells by differential centrifugation and discontinuous sucrose density gradient centrifugation. The properties of the proton pumping activity of H+-ATPases in tonoplast vesicles were studied by acridine orange fluorescent quenching measured at 22 degreesC. The proton pumping activity of ATPase was ATP-dependent with apparent Michaelis-Menten Constant (K-m) for ATP about 0.65 mmol/L. The optimal pH for H+-ATPases activity was 7.5. The proton pumping activity of H+-ATPase could be initiated by some divalent cations, Mg2+ being highly efficient, much more than Fe2+; and Ca2+, Cu2+ and Zn2+ were inefficient under the experimental condition. The proton translocation could be stimulated by halide anions, with potencies decreasing in the order Cl- > Br- > I- > F-. The proton pumping activity was greatly inhibited by N-ethylmaleimide (NEM), N, N'-dicyclohexylcarbodiimide (DCCD), NO3- and Bafilomycin A(1), but not by orthovanadate and azide. These results demonstrated that the H+-ATPase in the tonoplast of Populus euphratica belonged to vacuolar type ATPase. This work was the first time that tonoplast-enriched vesicles were isolated from Populus euphratica cells.

胡杨(Populus euphratica)叶片呼吸作用对地下水埋深的响应

土壤水分条件能够改变叶片呼吸作用,进而影响叶片、个体及生态系统的碳平衡。在内蒙古自治区额济纳旗境内,根据地下水埋深度的差异建立了干旱和湿润2块样地,并对其中生长的胡杨(Populus euphratica)叶片的呼吸作用特性进行了观测。结果表明,干旱样地胡杨叶片的相对含水量(RWC)和单位质量氮含量(Nmass)显著低于湿润样地,但叶片单位面积氮含量(Narea)和比叶面积(SLA)差异不显著。叶片暗呼吸速率(Rn)和光下线粒体呼吸速率(RL)均随地下水埋深的降低而升高,其中地下水埋深度的变化对RL影响更加明显。光对叶片暗呼吸速率具有抑制作用,其抑制程度在11%—57%变化。光对暗呼吸的抑制程度同Rubisco酶的最大氧化速率(vo)呈负相关。湿润样地胡杨叶片光合速率(A)同光下线粒体呼吸速率的比值(A/RL)显著高于A同Rn的比值(A/Rn),而在干旱样地2者间差异不显著。胡杨叶片呼吸速率和光对暗呼吸抑制作用对地下水埋深的响应可能同细胞抗旱对能量和物质需求的增加有关。此外,叶片A/R比值不仅受到光照条件的影响,同时受到水分条件的调控。

胡杨(Populus euphratica)盐相关基因的克隆

以 2年生胡杨实生苗为研究材料 ,通过用 119mmol L的NaCl溶液浇灌处理 1h ,分别收集NaCl处理前后的细嫩白根设立对照样品与处理样品 ,分离mRNA。用抑制性扣除杂交技术建立胡杨盐处理的cDNA扣除文库 ,并以cDNA阵列杂交技术用32 P标记探针进行杂交 ,共筛选 6 72个有效克隆 ,从中确认 3个探针进行了序列分析 ,其中 1个为盐抑制表达基因片段 ,2个为盐诱导表达基因片段。分析表明 ,1个盐诱导表达基因片段为 1含 188氨基酸开放阅读框的EST ,该EST具有 10 79个核苷酸。该结果与我们早先检测到的