The significant function of Toll-like receptors (TLR) is the detection of microbes by host guard cells that guide to the innate immune responses and to the successive adaptive. The current study patterns of TLR2, TLR3...The significant function of Toll-like receptors (TLR) is the detection of microbes by host guard cells that guide to the innate immune responses and to the successive adaptive. The current study patterns of TLR2, TLR3 and TLR9 expressing antigen presenting cells (APCs) in blood of mice after colonization with L. plantarum NC8 strain were assessed. The power of L. plantarum on serum innate cytokine and TLR responses stimulated by recombinant NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep and NC8-pSIP409-pgsA were also assessed. We confirmed that L. plantarum NC8 stimulated powerful TLR2 expressing APC responses in blood Recombinant strain stimulated a TLR3 response in spleen, and TLR9 responses were stimulated in blood or in spleen. Recombinant NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep on TLR2 and TLR9 expressing APC responses has a preservative outcome, reliable with the DCpep adjuvant outcome. In serum the recombinant NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep has increased the IL-4 and IFN-γ responses, except that on the TLR3 and TLR9 expressing CD14 APC responses it had an oppressive consequence in spleen and the IFN-α response in serum-stimulated by PRV. Our results give details that following PRV infection after immunization with NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep, the systemic TLR2, TLR3, and TLR9 expressing cDC and macrophage/monocyte responses.展开更多
A群轮状病毒(GAR)是引起人类和多种动物腹泻的主要病原体,病原的分离与鉴定是轮状病毒(RV)流行病学、病原学等研究的基础。本研究采用接种MA104细胞的方法,从内蒙古某猪场患病猪腹泻粪便中分离一株病毒,经3次蚀斑克隆纯化后,采用电镜观...A群轮状病毒(GAR)是引起人类和多种动物腹泻的主要病原体,病原的分离与鉴定是轮状病毒(RV)流行病学、病原学等研究的基础。本研究采用接种MA104细胞的方法,从内蒙古某猪场患病猪腹泻粪便中分离一株病毒,经3次蚀斑克隆纯化后,采用电镜观察、PCR鉴定和序列测定进行分析,结果表明该分离株为猪轮状病毒(PoRV)。致病性试验表明该分离株能够引起仔猪急性腹泻,RT-PCR扩增其VP4、VP6和VP7的基因节段并进行序列测定,按照A群RV的最新分类方法,确定该分离株的VP6、VP7和VP4基因型分别为I5型、G9型和P[23]型。因此,将该分离株命名为Rotavirus A pig/China/NMTL/2009/Q9P[23]。展开更多
[ Objectives ] To study the pathogenicity of rotavirns causing piglet diarrhea in Beijing area. [ Methods ] Six newborn piglets were randomly divided into two groups ： three piglets in test group and three in control...[ Objectives ] To study the pathogenicity of rotavirns causing piglet diarrhea in Beijing area. [ Methods ] Six newborn piglets were randomly divided into two groups ： three piglets in test group and three in control group. A total of 3 mL three-generation cell virus were evenly assigned to the three test piglets by both oral mode and subcutaneous injection, and the detoxification was observed. Anal swabs were collected at the 24^th, 48^th, 72^th and 96^th hours before and after the infection respectively, virus antigens were detected by RT-PCR, detoxlllcation was observed; small intestine, liver, spleen and other tissues of the 5-d-infected piglets were collected to observe the pathological changes. [ Results ] Anal swab detection showed that rotavirus could be detected in piglets at the 12^th after being in- fected, and the situation could continue to the 5^th d. Observation on tissue sections showed that piglet small intestinal epithelial cells and surrounding villi degenerated, epithelial cells became rounded, swollen; and enlarged nuclei and irregular cell margins could be observed. Degenerated cells fell off from matrix to lumen, at- rcphy villi was covered by flat epithelial cells, a small amount of cell debris could be observed in lamina propria. [ Conclusions ] The infection severity of porcine rotavirus depends on the atrophy degree and distribution of small intestinal villi. In addition, host, environment and other relevant factors also played an important role in the pathogenesis of porcine rotavirus.展开更多
文摘The significant function of Toll-like receptors (TLR) is the detection of microbes by host guard cells that guide to the innate immune responses and to the successive adaptive. The current study patterns of TLR2, TLR3 and TLR9 expressing antigen presenting cells (APCs) in blood of mice after colonization with L. plantarum NC8 strain were assessed. The power of L. plantarum on serum innate cytokine and TLR responses stimulated by recombinant NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep and NC8-pSIP409-pgsA were also assessed. We confirmed that L. plantarum NC8 stimulated powerful TLR2 expressing APC responses in blood Recombinant strain stimulated a TLR3 response in spleen, and TLR9 responses were stimulated in blood or in spleen. Recombinant NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep on TLR2 and TLR9 expressing APC responses has a preservative outcome, reliable with the DCpep adjuvant outcome. In serum the recombinant NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep has increased the IL-4 and IFN-γ responses, except that on the TLR3 and TLR9 expressing CD14 APC responses it had an oppressive consequence in spleen and the IFN-α response in serum-stimulated by PRV. Our results give details that following PRV infection after immunization with NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep, the systemic TLR2, TLR3, and TLR9 expressing cDC and macrophage/monocyte responses.
文摘A群轮状病毒(GAR)是引起人类和多种动物腹泻的主要病原体,病原的分离与鉴定是轮状病毒(RV)流行病学、病原学等研究的基础。本研究采用接种MA104细胞的方法,从内蒙古某猪场患病猪腹泻粪便中分离一株病毒,经3次蚀斑克隆纯化后,采用电镜观察、PCR鉴定和序列测定进行分析,结果表明该分离株为猪轮状病毒(PoRV)。致病性试验表明该分离株能够引起仔猪急性腹泻,RT-PCR扩增其VP4、VP6和VP7的基因节段并进行序列测定,按照A群RV的最新分类方法,确定该分离株的VP6、VP7和VP4基因型分别为I5型、G9型和P[23]型。因此,将该分离株命名为Rotavirus A pig/China/NMTL/2009/Q9P[23]。
基金Supported by Beijing"Vegetable Basket"Science and Technology ability upgrading project&Beijing Agricultural Vocational College Project(XY-YF-16-24)
文摘[ Objectives ] To study the pathogenicity of rotavirns causing piglet diarrhea in Beijing area. [ Methods ] Six newborn piglets were randomly divided into two groups ： three piglets in test group and three in control group. A total of 3 mL three-generation cell virus were evenly assigned to the three test piglets by both oral mode and subcutaneous injection, and the detoxification was observed. Anal swabs were collected at the 24^th, 48^th, 72^th and 96^th hours before and after the infection respectively, virus antigens were detected by RT-PCR, detoxlllcation was observed; small intestine, liver, spleen and other tissues of the 5-d-infected piglets were collected to observe the pathological changes. [ Results ] Anal swab detection showed that rotavirus could be detected in piglets at the 12^th after being in- fected, and the situation could continue to the 5^th d. Observation on tissue sections showed that piglet small intestinal epithelial cells and surrounding villi degenerated, epithelial cells became rounded, swollen; and enlarged nuclei and irregular cell margins could be observed. Degenerated cells fell off from matrix to lumen, at- rcphy villi was covered by flat epithelial cells, a small amount of cell debris could be observed in lamina propria. [ Conclusions ] The infection severity of porcine rotavirus depends on the atrophy degree and distribution of small intestinal villi. In addition, host, environment and other relevant factors also played an important role in the pathogenesis of porcine rotavirus.
