Optical coherence tomography(OCT)enables in vivo imaging of port wine stains(PWS)lesions.The knowledge of vascular struct ure and epidermal thickness(ET)of PWS may aid the objectivediagnosis and optimal treatment.To o...Optical coherence tomography(OCT)enables in vivo imaging of port wine stains(PWS)lesions.The knowledge of vascular struct ure and epidermal thickness(ET)of PWS may aid the objectivediagnosis and optimal treatment.To obtain the structural parameters more rapidly and avoiduser intervention,an automated algorithm of energy map is introduced based on intensity andedge information to extract the skin surface using dynamic programming method.Subsequently,an averaged A-scan analysis is performed to obtain the mean ET and the relative intensity ofdermis indicating the corresponding vascular density.This approach is currently successfullyapplied in clinical diagnosis and shows promising guidance and assessment of PDT treatment.展开更多
Vascular-targeted photodynamic therapy(V-PDT)is an effective treatment for port wine stains(PWS).However,repeated treatment is usually needed to achieve optimal treatment outcomes,possibly due to the limited treatment...Vascular-targeted photodynamic therapy(V-PDT)is an effective treatment for port wine stains(PWS).However,repeated treatment is usually needed to achieve optimal treatment outcomes,possibly due to the limited treatment light penetration depth in the PWS lesion.The optical clearing technique can increase light penetration in depth by reducing light scattering.This study aimed to investigate the V-PDT in combination with an optical clearing agent(OCA)for the therapeutic enhancement of V-PDT in the rodent skinfold window chamber model.Vascular responses were closely monitored with laser speckle contrast imaging(LSCI),optical coherence tomography angiography,and stereo microscope before,during,and after the treatment.We further quantitatively demonstrated the effects of V-PDT in combination with OCA on the blood flow and blood vessel size of skin microvasculature.The combination of OCA and V-PDT resulted in significant vascular damage,including vasoconstriction and the reduction of blood flow.Our results indicate the promising potential of OCA for enhancing V-PDT for treating vascular-related diseases,including PWS.展开更多
Background：The location of facial port-wine stain （PWS） may be helpful for predicting some associated anomalies;high glaucoma incidence is found in patients with eyes close to PWS-affected areas （V1,ophthalmic bra...Background：The location of facial port-wine stain （PWS） may be helpful for predicting some associated anomalies;high glaucoma incidence is found in patients with eyes close to PWS-affected areas （V1,ophthalmic branch area of the trigeminal nerve）.This study aimed to investigate the characteristics of glaucoma in V1-affected PWS.Methods：A total of 569 patients with V1 area-affected PWS were reviewed in the study.The large series was based on the referral system between the Department of Plastic and Reconstructive Surgery and the Department of Ophthalmology.All patients were screened for glaucoma with assessments of intraocular pressure,cup-to-disc ratio,comeal diameter （only for infants）,and axial length.Results：Of the 569 patients,110 （19.3%） patients had glaucoma.Among the patients,18.1% （76/420） had early-onset glaucoma （under 4-year-old group）.In the 4 to 18-year-old age group,29.3% （29/99） of the patients had glaucoma.Compared with right lateral and bilateral PWS,left-sided PWS had a lower risk of glaucoma in this study （odds ratio =0.432 [95% confidence interval,0.264-0.706],P =0.01）.The under 4-year-old group showed a slight predominance of males （61.8%） in glaucoma.Conclusions：High glaucoma incidence was observed in patients with eyes close to PWS.More attention should be paid to glaucoma screening for right lateral and bilateral PWS patients.The predominance of males in Sturge-Weber syndrome （SWS） early-onset glaucoma patients might be due to the limitation of the case number;however,it might also provide us a new clue of potential relationship between SWS and PCG.展开更多
基金supported in part by the National Natural Science Foundation of China under Grant 61227807by the Ministry of Science and Technology of China under contracts 2006AA02Z472,001CB510307 and 2009CB929400+1 种基金by the Ministryof Education of China Grant 20130002110079 for Doctoral Programby the Tsinghua Initiative Scientific Research Program Grant 2013THZ02-3.
文摘Optical coherence tomography(OCT)enables in vivo imaging of port wine stains(PWS)lesions.The knowledge of vascular struct ure and epidermal thickness(ET)of PWS may aid the objectivediagnosis and optimal treatment.To obtain the structural parameters more rapidly and avoiduser intervention,an automated algorithm of energy map is introduced based on intensity andedge information to extract the skin surface using dynamic programming method.Subsequently,an averaged A-scan analysis is performed to obtain the mean ET and the relative intensity ofdermis indicating the corresponding vascular density.This approach is currently successfullyapplied in clinical diagnosis and shows promising guidance and assessment of PDT treatment.
基金supported by the National Natural Science Foundation of China(Grant Numbers 62205025 and 61835015)Beijing Natural Science Foundation(7222309)+2 种基金the Open Project Program of Wuhan National Laboratory for Optoelectronics(2020WNLOKF025)CAMS Innovation Fund for Medical Sciences(CIFMS)(2019-I2M-5-061)Beijing Institute of Technology Research Fund Program for Young Scholars(XSQD-202123001).
文摘Vascular-targeted photodynamic therapy(V-PDT)is an effective treatment for port wine stains(PWS).However,repeated treatment is usually needed to achieve optimal treatment outcomes,possibly due to the limited treatment light penetration depth in the PWS lesion.The optical clearing technique can increase light penetration in depth by reducing light scattering.This study aimed to investigate the V-PDT in combination with an optical clearing agent(OCA)for the therapeutic enhancement of V-PDT in the rodent skinfold window chamber model.Vascular responses were closely monitored with laser speckle contrast imaging(LSCI),optical coherence tomography angiography,and stereo microscope before,during,and after the treatment.We further quantitatively demonstrated the effects of V-PDT in combination with OCA on the blood flow and blood vessel size of skin microvasculature.The combination of OCA and V-PDT resulted in significant vascular damage,including vasoconstriction and the reduction of blood flow.Our results indicate the promising potential of OCA for enhancing V-PDT for treating vascular-related diseases,including PWS.
基金This study was supported by grants from the National Natural Science Foundation （No. 81670845） and the Research Foundation of Shanghai Science and Technology Committee （No. 14411960600）.
文摘Background：The location of facial port-wine stain （PWS） may be helpful for predicting some associated anomalies;high glaucoma incidence is found in patients with eyes close to PWS-affected areas （V1,ophthalmic branch area of the trigeminal nerve）.This study aimed to investigate the characteristics of glaucoma in V1-affected PWS.Methods：A total of 569 patients with V1 area-affected PWS were reviewed in the study.The large series was based on the referral system between the Department of Plastic and Reconstructive Surgery and the Department of Ophthalmology.All patients were screened for glaucoma with assessments of intraocular pressure,cup-to-disc ratio,comeal diameter （only for infants）,and axial length.Results：Of the 569 patients,110 （19.3%） patients had glaucoma.Among the patients,18.1% （76/420） had early-onset glaucoma （under 4-year-old group）.In the 4 to 18-year-old age group,29.3% （29/99） of the patients had glaucoma.Compared with right lateral and bilateral PWS,left-sided PWS had a lower risk of glaucoma in this study （odds ratio =0.432 [95% confidence interval,0.264-0.706],P =0.01）.The under 4-year-old group showed a slight predominance of males （61.8%） in glaucoma.Conclusions：High glaucoma incidence was observed in patients with eyes close to PWS.More attention should be paid to glaucoma screening for right lateral and bilateral PWS patients.The predominance of males in Sturge-Weber syndrome （SWS） early-onset glaucoma patients might be due to the limitation of the case number;however,it might also provide us a new clue of potential relationship between SWS and PCG.
