The wingless-related integration site(WNT)proteins are a family of secreted glycoproteins that are evolutionarily conserved and are believed to be involved in evolution in vertebrates and invertebrates.WNT signaling p...The wingless-related integration site(WNT)proteins are a family of secreted glycoproteins that are evolutionarily conserved and are believed to be involved in evolution in vertebrates and invertebrates.WNT signaling pathways may be associated with limb regeneration and development in crustaceans.However,the detail mechanisms remain unclear.Therefore,the distribution of WNT4 in the hepatopancreas,muscle,hemocyte,ganglion,heart,eyestalk,gill tissue,and diff erent larvae development stages of the swimming crab(Portunus trituberculatus)were characterized using immunofl uorescence,real-time PCR,and Western blotting.Signifi cant PtWNT4 expression was detected in heart and eyestalk.In addition,PtWNT4 was expressed in all larval stages of P.trituberculatus with a dynamic expression pattern,especially in the eyestalk and other organs in the carapace area.The injection of WNT4 dsRNA into regenerative limbs signifi cantly decreased PtWNT4 mRNA levels in the eyestalk,heart,and muscle,resulting in 1.9-fold,2.2-fold,and 2.7-fold decreases compared with those detected in the group injected with crab saline(P<0.05),respectively,indicating successful gene silencing.Overall,expression analysis on the WNT4 using RNAi provides an insight to its functional mechanism during limb regeneration in P.trituberculatus.The results not only demonstrated the requirement for WNT4 in limb regeneration of Crustaceans,but also suggested its ability to promote larval development at specifi c stages.展开更多
通过观察不同饥饿胁迫处理对三疣梭子蟹(Portunus trituberculatus)溞状Ⅰ期幼体变态和发育的影响,研究了其"营养储存饱和点"(the point of reserve saturation,PRS)和"不可恢复点"(the point of no return,PNR)现...通过观察不同饥饿胁迫处理对三疣梭子蟹(Portunus trituberculatus)溞状Ⅰ期幼体变态和发育的影响,研究了其"营养储存饱和点"(the point of reserve saturation,PRS)和"不可恢复点"(the point of no return,PNR)现象的发生情况。结果表明:以轮虫为饵料,三疣梭子蟹溞状Ⅰ期幼体(ZⅠ)在前期投喂后期饥饿的条件下,随着前期摄食时间的增加,幼体变态率和发育速度呈上升趋势,50%营养储存饱和点(PRS50)出现在47.5 h,100%营养储存饱和点(PRS100)出现在72 h;在前期饥饿后期投喂的条件下,随着前期饥饿时间的增加,幼体变态率和发育速度呈下降趋势,50%不可恢复点(PNR50)出现在39 h,100%不可恢复点(PNR100)出现在56 h。展开更多
基金Supported by the National Natural Science Foundation of China(No.31602152)the Major Agriculture Program of Ningbo(No.2017C110007)the K.C.Wong Magana Fund in Ningbo University.The funding body had no role in the study design,experimental implementation,interpretation of data,or writing of the manuscript。
文摘The wingless-related integration site(WNT)proteins are a family of secreted glycoproteins that are evolutionarily conserved and are believed to be involved in evolution in vertebrates and invertebrates.WNT signaling pathways may be associated with limb regeneration and development in crustaceans.However,the detail mechanisms remain unclear.Therefore,the distribution of WNT4 in the hepatopancreas,muscle,hemocyte,ganglion,heart,eyestalk,gill tissue,and diff erent larvae development stages of the swimming crab(Portunus trituberculatus)were characterized using immunofl uorescence,real-time PCR,and Western blotting.Signifi cant PtWNT4 expression was detected in heart and eyestalk.In addition,PtWNT4 was expressed in all larval stages of P.trituberculatus with a dynamic expression pattern,especially in the eyestalk and other organs in the carapace area.The injection of WNT4 dsRNA into regenerative limbs signifi cantly decreased PtWNT4 mRNA levels in the eyestalk,heart,and muscle,resulting in 1.9-fold,2.2-fold,and 2.7-fold decreases compared with those detected in the group injected with crab saline(P<0.05),respectively,indicating successful gene silencing.Overall,expression analysis on the WNT4 using RNAi provides an insight to its functional mechanism during limb regeneration in P.trituberculatus.The results not only demonstrated the requirement for WNT4 in limb regeneration of Crustaceans,but also suggested its ability to promote larval development at specifi c stages.
文摘通过观察不同饥饿胁迫处理对三疣梭子蟹(Portunus trituberculatus)溞状Ⅰ期幼体变态和发育的影响,研究了其"营养储存饱和点"(the point of reserve saturation,PRS)和"不可恢复点"(the point of no return,PNR)现象的发生情况。结果表明:以轮虫为饵料,三疣梭子蟹溞状Ⅰ期幼体(ZⅠ)在前期投喂后期饥饿的条件下,随着前期摄食时间的增加,幼体变态率和发育速度呈上升趋势,50%营养储存饱和点(PRS50)出现在47.5 h,100%营养储存饱和点(PRS100)出现在72 h;在前期饥饿后期投喂的条件下,随着前期饥饿时间的增加,幼体变态率和发育速度呈下降趋势,50%不可恢复点(PNR50)出现在39 h,100%不可恢复点(PNR100)出现在56 h。
