Sequential ultrasonograms of the ccrvix and uterus were taken in both cross and longitudinal sections at different periods of involution in eleven postparturn Jersey cows, and in the meantime the concentrations of ...Sequential ultrasonograms of the ccrvix and uterus were taken in both cross and longitudinal sections at different periods of involution in eleven postparturn Jersey cows, and in the meantime the concentrations of ocstndiol-17β and progesterone in peripheral circulation of the cows were measured by radioimmunoassay(RIA). Cervical involution was showed in detail by using ultrasound scanner during its whole involuting phase, and the oestrus could be indicated by the differences of the ultrasonograms of the genital tract at the time. It took 46.7+3.9 days for the cervix to complete involution after calving, and its involuting rate was delayed by the occurence of the periparturient fatty liver and injury of the cervix during parturtion.展开更多
Two postpartum cows were used to study the ultrastructural changes of uterine endometrium by using scanning electron microscope.The results showed that the process of repair of uterine endometrium after calving was de...Two postpartum cows were used to study the ultrastructural changes of uterine endometrium by using scanning electron microscope.The results showed that the process of repair of uterine endometrium after calving was demonstrated by scanning electron microscope through a series of endometrium biopsy.Some part of the endometrium was damaged after calving and its adjacent endometrium cells became necrosis and exfoliated during the first 7 days post-partum;the cilium and microvillus of the epithelial cell in the undamaged area of the endometrium disappeared.By 26 days postpartum the damaged area reduced and the cilium and microvillus increased in their numbers.The damaged tissues were all repaired by day 60 postpartum.展开更多
Ovarian activitly in 10 postpartum Chinese-Holstein cows was monitored by ultrasonography every other day, stared from day 3 postpartum and finished by the end of a normal ovarian cycle. Meanwhile, the concentrations ...Ovarian activitly in 10 postpartum Chinese-Holstein cows was monitored by ultrasonography every other day, stared from day 3 postpartum and finished by the end of a normal ovarian cycle. Meanwhile, the concentrations of progesterone and 17β-oestradiol in peripheral plasma of the cows were measured by radioimmunoassay (RIA). The resutis showed that the first postpartum ovulation was found in 67% of the cows on the ovary opposed to the gravid horn. The first dominant foillicles ovulated for the six of ten cows. The time intervals from calving to the first ovulation were significantly associated with the number of follicular waves (r=0.83, P<0.01). No difference between the diameters of the first and the second ovulated follicles was found in the study, however, the growth rates of the first and the second ovulated follicles were significantly different (P<0.05), and the concentrations of 17β-oestradiol prior to the first ovulation were significantly lower than that before the second ovulation.展开更多
To investigate the roles of Escherichia coli in the pathogenesis of postpartum uterine diseases in dairy cows, a total of 145 E. coli isolates were recovered from 18 healthy cows （61 isolates） and 25 cows with clini...To investigate the roles of Escherichia coli in the pathogenesis of postpartum uterine diseases in dairy cows, a total of 145 E. coli isolates were recovered from 18 healthy cows （61 isolates） and 25 cows with clinical endometritis （84 isolates） at 25-35 days after parturition. Genomic characteristics including phylogenetic grouping, genetic diversity and virulence genes of E. coli isolates were screened to profile the characteristics related to uterine infections. The susceptibility of the bacteria against 23 antibiotics was also evaluated to support prevention and treatment of clinical cases. Genetic diversity of E. coli identified by random amplification of polymorphic DNA （RAPD） revealed 103 clonal types, including 3 common types to unaffected cows and endometritis cows, 39 types specific to healthy cows and 61 types in endometritis subjects. In addition, the isolates from endometritis uteri showed more genetic variability compared with that of healthy cows. Ac- cording to the findings of phylogenetic grouping, the E. coil isolates were assigned to group A （35.9%）, B1 （59.3%） and D （4.8%）. The expression of 10 of 20 virulence gens were detected positively, and only fimH gene was revealed significantly （P〈0.05） associated with endometritis. From antimicrobial susceptibility test, E. coli was found highly resistant to tetracy- cline, ampicillin, carbenicillin and amoxicillin, but sensitive to amikacin, netilmicin, tobramycin, cefepime and ceftazidime. In conclusion, E. cofiwere extensively observed in both healthy and endometritis cows, and presented a large clonal types, however, fimH was the only gene observed associated with clinical endometritis. Our results suggest that the drugs like amikacin, netilmicin, tobramycin and cefepime could be considered for preventing and treating clinical endometritis in the practical management of dairy cow.展开更多
文摘Sequential ultrasonograms of the ccrvix and uterus were taken in both cross and longitudinal sections at different periods of involution in eleven postparturn Jersey cows, and in the meantime the concentrations of ocstndiol-17β and progesterone in peripheral circulation of the cows were measured by radioimmunoassay(RIA). Cervical involution was showed in detail by using ultrasound scanner during its whole involuting phase, and the oestrus could be indicated by the differences of the ultrasonograms of the genital tract at the time. It took 46.7+3.9 days for the cervix to complete involution after calving, and its involuting rate was delayed by the occurence of the periparturient fatty liver and injury of the cervix during parturtion.
基金The projectis supported financially by Heilongjiang( C- 970 2 ) and Chinese national( 39870 572 ) natural and scientificfunds.
文摘Two postpartum cows were used to study the ultrastructural changes of uterine endometrium by using scanning electron microscope.The results showed that the process of repair of uterine endometrium after calving was demonstrated by scanning electron microscope through a series of endometrium biopsy.Some part of the endometrium was damaged after calving and its adjacent endometrium cells became necrosis and exfoliated during the first 7 days post-partum;the cilium and microvillus of the epithelial cell in the undamaged area of the endometrium disappeared.By 26 days postpartum the damaged area reduced and the cilium and microvillus increased in their numbers.The damaged tissues were all repaired by day 60 postpartum.
文摘Ovarian activitly in 10 postpartum Chinese-Holstein cows was monitored by ultrasonography every other day, stared from day 3 postpartum and finished by the end of a normal ovarian cycle. Meanwhile, the concentrations of progesterone and 17β-oestradiol in peripheral plasma of the cows were measured by radioimmunoassay (RIA). The resutis showed that the first postpartum ovulation was found in 67% of the cows on the ovary opposed to the gravid horn. The first dominant foillicles ovulated for the six of ten cows. The time intervals from calving to the first ovulation were significantly associated with the number of follicular waves (r=0.83, P<0.01). No difference between the diameters of the first and the second ovulated follicles was found in the study, however, the growth rates of the first and the second ovulated follicles were significantly different (P<0.05), and the concentrations of 17β-oestradiol prior to the first ovulation were significantly lower than that before the second ovulation.
基金supported by the National Key Technology R&D Program of China (2011BAD19B02)
文摘To investigate the roles of Escherichia coli in the pathogenesis of postpartum uterine diseases in dairy cows, a total of 145 E. coli isolates were recovered from 18 healthy cows （61 isolates） and 25 cows with clinical endometritis （84 isolates） at 25-35 days after parturition. Genomic characteristics including phylogenetic grouping, genetic diversity and virulence genes of E. coli isolates were screened to profile the characteristics related to uterine infections. The susceptibility of the bacteria against 23 antibiotics was also evaluated to support prevention and treatment of clinical cases. Genetic diversity of E. coli identified by random amplification of polymorphic DNA （RAPD） revealed 103 clonal types, including 3 common types to unaffected cows and endometritis cows, 39 types specific to healthy cows and 61 types in endometritis subjects. In addition, the isolates from endometritis uteri showed more genetic variability compared with that of healthy cows. Ac- cording to the findings of phylogenetic grouping, the E. coil isolates were assigned to group A （35.9%）, B1 （59.3%） and D （4.8%）. The expression of 10 of 20 virulence gens were detected positively, and only fimH gene was revealed significantly （P〈0.05） associated with endometritis. From antimicrobial susceptibility test, E. coli was found highly resistant to tetracy- cline, ampicillin, carbenicillin and amoxicillin, but sensitive to amikacin, netilmicin, tobramycin, cefepime and ceftazidime. In conclusion, E. cofiwere extensively observed in both healthy and endometritis cows, and presented a large clonal types, however, fimH was the only gene observed associated with clinical endometritis. Our results suggest that the drugs like amikacin, netilmicin, tobramycin and cefepime could be considered for preventing and treating clinical endometritis in the practical management of dairy cow.
