[ Objective] This study aimed to screen and identify tobacco mutants resistant to potato virus Y （PVY）, thus laying the foundation for obtaining PVY resistance genes. [ Method ] At seedling stage, tobacco mutant mat...[ Objective] This study aimed to screen and identify tobacco mutants resistant to potato virus Y （PVY）, thus laying the foundation for obtaining PVY resistance genes. [ Method ] At seedling stage, tobacco mutant materials were inoculated with PVY virus and preliminarily screened by naked-eye observation. Enzyme-linked immunosorbent assay （ELISA）, real-time fluorescence quantitative PCR and test strip assay were performed to further identify the pre-screened PVY- resistant seedlings. [ Result ] In 2011, two highly PVY-resistant tobacco mutants （MZE2-15 and MZE2-16） and three PVY-tolerant mutants （MZE2-70, MZE2- 207 and MZE2-228） were obtained, which were further screened and identified in 2012. According to the results, tobacco mutant materials MZE2-407 and MZE2- 428 were susceptible to PVY; mutant materials MZE2-16 and MZE2-15 were resistant to PVY. [ Conclusion] This study provide theoretical basis for the control of tobacco PVY disease.展开更多
Potato virus Y(PVY)is a non-persistent virus that is transmitted by many aphid species and causes significant damage to potato production.We constructed a spatially-explicit model simulating PVY spread in a potato fie...Potato virus Y(PVY)is a non-persistent virus that is transmitted by many aphid species and causes significant damage to potato production.We constructed a spatially-explicit model simulating PVY spread in a potato field and used it to investigate possible effects of transmission efficiency,initial inoculum levels,vector behavior,vector abundance,and timing of peak vector activity on PVY incidence at the end of a simulated growing season.Lower PVY incidence in planted seed resulted in lower virus infection at the end of the season.However,when populations of efficient PVY vectors were high,significant PVY spread occurred even when initial virus inoculum was low.Non-colonizing aphids were more important for PVY spread compared to colonizing aphids,particularly at high densities.An early-season peak in the numbers of noncolonizing aphids resulted in the highest number of infected plants in the end of the season,while mid-and late-season peaks caused relatively little virus spread.Our results highlight the importance of integrating different techniques to prevent the number of PVY-infected plants from exceeding economically acceptable levels instead of trying to control aphids within potato fields.Such management plans should be implemented very early in a growing season.展开更多
马铃薯Y病毒(Potato virus Y,PVY)是危害烟草的重要病害,种植抗病品种是经济有效的防治措施。分子标记辅助选择可提高抗病育种效率。来源于X-射线诱变的Virgin A Mutante(VAM)的隐性抗PVY基因位点(va)被广泛应用于烟草抗病育种。为了提...马铃薯Y病毒(Potato virus Y,PVY)是危害烟草的重要病害,种植抗病品种是经济有效的防治措施。分子标记辅助选择可提高抗病育种效率。来源于X-射线诱变的Virgin A Mutante(VAM)的隐性抗PVY基因位点(va)被广泛应用于烟草抗病育种。为了提高va位点的育种利用效率,根据烟草隐性抗PVY基因(感病基因)e IF4E-1基因序列,设计特异扩增的引物CF2GR11,开发e IF4E-1基因的分子标记,并检测了该标记与抗性的遗传距离和在常见烟草资源中的适用性。CF2GR11在云烟87、红花大金元和K326等感PVY品种可扩增出500 bp产物,在NC102、NC55和K326PVY等抗病品种无扩增条带。以抗、感PVY亲本构建的101个F2单株为定位群体,遗传连锁分析表明,CF2GR11标记与烟草PVY感病基因的遗传距离为0.99 c M。对46份PVY抗性明确的栽培烟草资源的检测表明,供试资源的标记检测结果与抗性的吻合度为100%,表明CF2GR11标记适用性高。该目的基因标记可用于抗PVY育种的辅助选择和抗PVY资源的鉴定。展开更多
基金Supported by Major Mutant Program of National Tobacco Genome Project of China[110201201004(JY-04)]
文摘[ Objective] This study aimed to screen and identify tobacco mutants resistant to potato virus Y （PVY）, thus laying the foundation for obtaining PVY resistance genes. [ Method ] At seedling stage, tobacco mutant materials were inoculated with PVY virus and preliminarily screened by naked-eye observation. Enzyme-linked immunosorbent assay （ELISA）, real-time fluorescence quantitative PCR and test strip assay were performed to further identify the pre-screened PVY- resistant seedlings. [ Result ] In 2011, two highly PVY-resistant tobacco mutants （MZE2-15 and MZE2-16） and three PVY-tolerant mutants （MZE2-70, MZE2- 207 and MZE2-228） were obtained, which were further screened and identified in 2012. According to the results, tobacco mutant materials MZE2-407 and MZE2- 428 were susceptible to PVY; mutant materials MZE2-16 and MZE2-15 were resistant to PVY. [ Conclusion] This study provide theoretical basis for the control of tobacco PVY disease.
基金supported in part by the United States Department of Agriculture National institute of Food and Agriculture Special Crops Research initiative (Award # 2014-51181-22373)Funding for Hongchun Qu’s stay at the University of Maine was received from the National Natural Science Foundation of China (Award # 61871061)
文摘Potato virus Y(PVY)is a non-persistent virus that is transmitted by many aphid species and causes significant damage to potato production.We constructed a spatially-explicit model simulating PVY spread in a potato field and used it to investigate possible effects of transmission efficiency,initial inoculum levels,vector behavior,vector abundance,and timing of peak vector activity on PVY incidence at the end of a simulated growing season.Lower PVY incidence in planted seed resulted in lower virus infection at the end of the season.However,when populations of efficient PVY vectors were high,significant PVY spread occurred even when initial virus inoculum was low.Non-colonizing aphids were more important for PVY spread compared to colonizing aphids,particularly at high densities.An early-season peak in the numbers of noncolonizing aphids resulted in the highest number of infected plants in the end of the season,while mid-and late-season peaks caused relatively little virus spread.Our results highlight the importance of integrating different techniques to prevent the number of PVY-infected plants from exceeding economically acceptable levels instead of trying to control aphids within potato fields.Such management plans should be implemented very early in a growing season.
文摘马铃薯Y病毒(Potato virus Y,PVY)是危害烟草的重要病害,种植抗病品种是经济有效的防治措施。分子标记辅助选择可提高抗病育种效率。来源于X-射线诱变的Virgin A Mutante(VAM)的隐性抗PVY基因位点(va)被广泛应用于烟草抗病育种。为了提高va位点的育种利用效率,根据烟草隐性抗PVY基因(感病基因)e IF4E-1基因序列,设计特异扩增的引物CF2GR11,开发e IF4E-1基因的分子标记,并检测了该标记与抗性的遗传距离和在常见烟草资源中的适用性。CF2GR11在云烟87、红花大金元和K326等感PVY品种可扩增出500 bp产物,在NC102、NC55和K326PVY等抗病品种无扩增条带。以抗、感PVY亲本构建的101个F2单株为定位群体,遗传连锁分析表明,CF2GR11标记与烟草PVY感病基因的遗传距离为0.99 c M。对46份PVY抗性明确的栽培烟草资源的检测表明,供试资源的标记检测结果与抗性的吻合度为100%,表明CF2GR11标记适用性高。该目的基因标记可用于抗PVY育种的辅助选择和抗PVY资源的鉴定。
