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Effects of Different Wall-breaking Methods on Extraction of Solanesol from Stems and Leaves of Potato
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作者 Bingpeng WANG Jing MENG +3 位作者 Mei GUO Xinyi HUANG Fangyuan DONG Duolong DI 《Agricultural Biotechnology》 CAS 2017年第2期11-14,共4页
In order to extract solanesol from potato stems and leaves more effectively and improve the extraction rate of solanesol, the same batch of potato stems and leaves harvested from Guyuan, Ningxia was selected as a rese... In order to extract solanesol from potato stems and leaves more effectively and improve the extraction rate of solanesol, the same batch of potato stems and leaves harvested from Guyuan, Ningxia was selected as a research object, cell wall of potato stems and leaves was broken by enzymolysis with cellulase and high- speed shearing, and then reflux-extracted with 95% ethanol. Solanesol content was determined by HPLC, and extract yield was calculated. Extract yield and solanesol extraction rate was used as an index for comparison of difference between the 2 wall-breaking methods, so as to select the optimal wall-breaking method. The results showed that enzymolysis with cellulase exhibited extraction rate and extract yield of solanesol of 91.38% and 8.02%, respectively, which were better than those under high-speed shear emulsification technique. The enzymolysis wall-breaking method has the advantages of simple operation and strong feasibility. 展开更多
关键词 ENZYMOLYSIS High-speed shearing potato stems and leaves SOLANESOL
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