This paper presents a novel biosensor for bitter substance detection on the basis of light addressable potentiometric sensor(LAPS).Taste receptor cells(TRCs)were used as sensitive elements,which can respond to differe...This paper presents a novel biosensor for bitter substance detection on the basis of light addressable potentiometric sensor(LAPS).Taste receptor cells(TRCs)were used as sensitive elements,which can respond to different bitter stimuli with extreme high sensitivity and speci-ficity.TRCs were isolated from the taste buds of rats and cultured on the surface of LAPS chip.Due to the unique advantages such as single-cell recording,light addressable capability,and noninvasiveness,LAPS chip was used as secondary transducer to monitor the responses of TRCs by recording extracelluar potential changes.The results indicate LAPS chip can effectively record the responses of TRCs to different bitter substances used in this study in a real-time manner for a long-term.In addition,by performing principal component analysis on the LAPS recording data,different bitter substances tested can be successfully discriminated.It is suggested this TRCsLAPS hybrid biosensor could be a valuable tool for bitter substance detection.With further improvement and novel design,it has great potentials to be applied in both basic research and practical applications related to bitter taste detection.展开更多
<正>A novel neurochip based on light addressable potentiometric sensor (LAPS) is designed.Using its light addressable characteristic.The problems of the limitations of restricted discrete active sites of current...<正>A novel neurochip based on light addressable potentiometric sensor (LAPS) is designed.Using its light addressable characteristic.The problems of the limitations of restricted discrete active sites of current neurochips,such as microelectrode array and field effect transistor array can be settled easily.Based on the theoretical analysis of the interface between cells and LAPS,spontaneously discharges of hippocampal neurons induced by Mg~ 2+)-free media treatment were recorded by LAPS.The results demonstrate that this kind of neurochip has potential to monitor electrophysiology of cultured cells in a non-invasive way.展开更多
The light-addressable potentiometric sensor (LAPS) is a semiconductor-based cellular biosensor with an electrolyte-insulator-semiconductor (EIS) structure.By depositing biocompatible layers on the sensing surface fo...The light-addressable potentiometric sensor (LAPS) is a semiconductor-based cellular biosensor with an electrolyte-insulator-semiconductor (EIS) structure.By depositing biocompatible layers on the sensing surface for cell culture, it can be used to detect bioelectrical parameters of cells.The characteristic curve for photocurrent versus applied bias voltage to the system shows a current-voltage curve (Ⅰ-Ⅴcurve).This technique can be used to detect the action potential changes towards different drugs based on the bias voltage dependence of an optical current,and provides a dynamic system by scanning light beam at the very cell on the sensor device.The LAPS overcomes the limitation of recording sites,but high spatial resolution and sensitivity are also paramount.This paper discussed a novel structure of LAPS array for extracellular monitoring to decrease potential noise level.Both characteristics of active recording array areas and cell culture conditions are measured.展开更多
The authors developed a nitrate ion-selective electrode(ISE) based on poly(vinyl chloride)(PVC) membrane with methyltrioctylammonium nitrate as a carrier and 1-decanol as a plasticizer.The performance of the nit...The authors developed a nitrate ion-selective electrode(ISE) based on poly(vinyl chloride)(PVC) membrane with methyltrioctylammonium nitrate as a carrier and 1-decanol as a plasticizer.The performance of the nitrate-sensitive membranes was optimized by tuning the composition of components.The electrode exhibits a linear response with a Nernstian slope of(52±1.0) mV per decade for the nitrate ion concentration ranging from 5.8×10-5 mol/L to 1.0 mol/L.The electrode can be used to detect a low concentration of nitrate ions down to 3×10-5 mol/L in a pH range of 2.1―11.5 without any compensation.The advantage of the electrode includes simple preparation,short response time and good repeatability.The detection performance of the novel electrode on nitrate ions has been tested for water samples.展开更多
In this study,a multisensor system consisting of 23 potentiometric sensors was applied for long-term online measurements in outlet flow of the water treatment plant.Within 1 month of continuous measurements,the data s...In this study,a multisensor system consisting of 23 potentiometric sensors was applied for long-term online measurements in outlet flow of the water treatment plant.Within 1 month of continuous measurements,the data set of more than 295,000 observations was acquired.The processing of this dataset with conventional chemometric tools was cumbersome and not very informative.Topological data analysis(TDA)was recently suggested in chemometric literature to deal with large spectroscopic datasets.In this research,we explore the opportunities of TDA with respect to multisensor data with only 23 variables.It is shown that TDA allows for convenient data visualization,studying the evolution of water quality during the measurements and tracking the periodical structure in the data related to the water quality depending on the time of the day and the day of the week.TDA appears to be a valuable tool for multisensor data exploration.展开更多
The variety of newly synthesized diamides of dipicolinic acid(DPA)bearing methyl-and oxymethyl substituents in various positions of phenyl ring was investigated as potential ligands for liquid-liquid extraction of rar...The variety of newly synthesized diamides of dipicolinic acid(DPA)bearing methyl-and oxymethyl substituents in various positions of phenyl ring was investigated as potential ligands for liquid-liquid extraction of rare earth metal ions and as membrane active compounds in plasticized polymeric membranes of potentiometric sensors.It is found that scandium is extracted with 2,6-pyridinedicarboxylic acid diamides similarly to heavy lanthanides,while yttrium is extracted together with light group lanthanides.DPA shows high selectivity for the separation of cadmium/zinc,lead/nickel or copper/nickel pairs.The four out of six studied ligands demonstrate significant potentiometric sensitivity towards scandium,yttrium and lanthanides in 10^(-7)-10^(-3)mol/L concentration range in nitric acid solutions.The trends in sensitivity values vary depending on a ligand structure and the highest values are observed for Sc^(3+)and heavy lanthanides.According to the evaluated selectivity Sm^(3+)is the most preferred ion,while La^(3+)is the most discriminated.The differences in sensor behavior in rare earth metal solutions assume that such devices can be applied in sensor arrays for potentiometric analysis of lanthanide mixtures.展开更多
基金This work was supported by the grants from the National Natural Science Foundation of China(Grant Nos.60725102,31000448)the China Postdoctoral Science Foundation(Grant Nos.20100471737,201104734)the Key Project of Zhejiang Province(Grant No.2010C14006).
文摘This paper presents a novel biosensor for bitter substance detection on the basis of light addressable potentiometric sensor(LAPS).Taste receptor cells(TRCs)were used as sensitive elements,which can respond to different bitter stimuli with extreme high sensitivity and speci-ficity.TRCs were isolated from the taste buds of rats and cultured on the surface of LAPS chip.Due to the unique advantages such as single-cell recording,light addressable capability,and noninvasiveness,LAPS chip was used as secondary transducer to monitor the responses of TRCs by recording extracelluar potential changes.The results indicate LAPS chip can effectively record the responses of TRCs to different bitter substances used in this study in a real-time manner for a long-term.In addition,by performing principal component analysis on the LAPS recording data,different bitter substances tested can be successfully discriminated.It is suggested this TRCsLAPS hybrid biosensor could be a valuable tool for bitter substance detection.With further improvement and novel design,it has great potentials to be applied in both basic research and practical applications related to bitter taste detection.
基金supported by the National Natural Science Foundation of China(Grant Nos.30270387,No.30570492)the Project of State Key Laboratory of Transducer Technology of China(Grant No.SKT0403)the Foundation for the Bureau of Zhejiang Province of China(Grant No.20040197).
文摘<正>A novel neurochip based on light addressable potentiometric sensor (LAPS) is designed.Using its light addressable characteristic.The problems of the limitations of restricted discrete active sites of current neurochips,such as microelectrode array and field effect transistor array can be settled easily.Based on the theoretical analysis of the interface between cells and LAPS,spontaneously discharges of hippocampal neurons induced by Mg~ 2+)-free media treatment were recorded by LAPS.The results demonstrate that this kind of neurochip has potential to monitor electrophysiology of cultured cells in a non-invasive way.
基金This work was supported by the National Natural Science Foundation of China (Grant Nos. 30270387, No. 30570492);the Project of State Key Laboratory of Transducer Technology of China (Grant No. SKT0403);the Foundation for the Bureau of Zhejiang Province of China (Grant No. 20040197).
文摘The light-addressable potentiometric sensor (LAPS) is a semiconductor-based cellular biosensor with an electrolyte-insulator-semiconductor (EIS) structure.By depositing biocompatible layers on the sensing surface for cell culture, it can be used to detect bioelectrical parameters of cells.The characteristic curve for photocurrent versus applied bias voltage to the system shows a current-voltage curve (Ⅰ-Ⅴcurve).This technique can be used to detect the action potential changes towards different drugs based on the bias voltage dependence of an optical current,and provides a dynamic system by scanning light beam at the very cell on the sensor device.The LAPS overcomes the limitation of recording sites,but high spatial resolution and sensitivity are also paramount.This paper discussed a novel structure of LAPS array for extracellular monitoring to decrease potential noise level.Both characteristics of active recording array areas and cell culture conditions are measured.
基金Supported by the Project of Wuxi Pollution Prevention Funding Agency,China(No.2008-1)
文摘The authors developed a nitrate ion-selective electrode(ISE) based on poly(vinyl chloride)(PVC) membrane with methyltrioctylammonium nitrate as a carrier and 1-decanol as a plasticizer.The performance of the nitrate-sensitive membranes was optimized by tuning the composition of components.The electrode exhibits a linear response with a Nernstian slope of(52±1.0) mV per decade for the nitrate ion concentration ranging from 5.8×10-5 mol/L to 1.0 mol/L.The electrode can be used to detect a low concentration of nitrate ions down to 3×10-5 mol/L in a pH range of 2.1―11.5 without any compensation.The advantage of the electrode includes simple preparation,short response time and good repeatability.The detection performance of the novel electrode on nitrate ions has been tested for water samples.
基金The authors are grateful to O.Lominoga and Zh.Lyadova from SUE“Vodokanal of St.Petersburg”for their valuable help in organizing the experiments.DK acknowledges financial support from RFBR project#17-33-50101.EL and AL acknowledge partial financial support from the Government of Russian Federation,Grant 08-08.VB thanks the Russian Ministry of Education and Science for support of this work within the framework of the basic part of the state task on the theme:“Adaptive technologies of analytical control based on optical sensors”(Project No.4.7001.2017/BP).
文摘In this study,a multisensor system consisting of 23 potentiometric sensors was applied for long-term online measurements in outlet flow of the water treatment plant.Within 1 month of continuous measurements,the data set of more than 295,000 observations was acquired.The processing of this dataset with conventional chemometric tools was cumbersome and not very informative.Topological data analysis(TDA)was recently suggested in chemometric literature to deal with large spectroscopic datasets.In this research,we explore the opportunities of TDA with respect to multisensor data with only 23 variables.It is shown that TDA allows for convenient data visualization,studying the evolution of water quality during the measurements and tracking the periodical structure in the data related to the water quality depending on the time of the day and the day of the week.TDA appears to be a valuable tool for multisensor data exploration.
文摘The variety of newly synthesized diamides of dipicolinic acid(DPA)bearing methyl-and oxymethyl substituents in various positions of phenyl ring was investigated as potential ligands for liquid-liquid extraction of rare earth metal ions and as membrane active compounds in plasticized polymeric membranes of potentiometric sensors.It is found that scandium is extracted with 2,6-pyridinedicarboxylic acid diamides similarly to heavy lanthanides,while yttrium is extracted together with light group lanthanides.DPA shows high selectivity for the separation of cadmium/zinc,lead/nickel or copper/nickel pairs.The four out of six studied ligands demonstrate significant potentiometric sensitivity towards scandium,yttrium and lanthanides in 10^(-7)-10^(-3)mol/L concentration range in nitric acid solutions.The trends in sensitivity values vary depending on a ligand structure and the highest values are observed for Sc^(3+)and heavy lanthanides.According to the evaluated selectivity Sm^(3+)is the most preferred ion,while La^(3+)is the most discriminated.The differences in sensor behavior in rare earth metal solutions assume that such devices can be applied in sensor arrays for potentiometric analysis of lanthanide mixtures.