Effects of thrombopoietin pre-treatment on peri-liver transplantation thrombocytopenia in a mouse model of cirrhosis with hypersplenism

BACKGROUND During cirrhosis,the liver is impaired and unable to synthesize and clear thrombopoietin properly.At the same time,the spleen assumes the function of hemofiltration and storage due to liver dysfunction,resulting in hypersplenism and excessive removal of platelets in the spleen,further reducing platelet count.When liver function is decompensated in cirrhotic patients,the decrease of thrombopoietin(TPO)synthesis is the main reason for the decrease of new platelet production.This change of TPO leads to thrombocytopenia and bleeding tendency in cirrhotic patients with hypersplenism.AIM To investigate the clinical efficacy of recombinant human TPO(rhTPO)in the treatment of perioperative thrombocytopenia during liver transplantation in cirrhotic mice with hypersplenism.METHODS C57BL/6J mice and TPO receptor-deficient mice were used to establish models of cirrhosis with hypersplenism.Subsequently,these mice underwent orthotopic liver transplantation(OLT).The mice in the experimental group were given rhTPO treatment for 3 consecutive days before surgery and 5 consecutive days after surgery,while the mice in the control group received the same dose of saline at the same frequency.Differences in liver function and platelet counts were determined between the experimental and control groups.Enzyme-linked immunosorbent assay was used to assess the expression of TPO and TPO receptor(c-Mpl)in the blood.RESULTS Preoperative administration of rhTPO significantly improved peri-OLT thrombocytopenia in mice with cirrhosis and hypersplenism.Blocking the expression of TPO receptors exacerbated peri-OLT thrombocytopenia.The concentration of TPO decreased while the concentration of c-Mpl increased in compensation in the mouse model of cirrhosis with hypersplenism.TPO pre-treatment significantly increased the postoperative TPO concentration in mice,which in turn led to a decrease in the c-Mpl concentration.TPO pre-treatment also significantly enhanced the Janus kinase(Jak)/signal transducers and activators of transcription pathway protein expressions in bone marrow stem cells of the C57BL/6J mice.Moreover,the administration of TPO,both before and after surgery,regulated the levels of biochemical indicators,such as alanine aminotransferase,alkaline phosphatase,and aspartate aminotransferase in the C57BL/6J mice.CONCLUSION Pre-treatment with TPO not only exhibited therapeutic effects on perioperative thrombocytopenia in the mice with cirrhosis and hypersplenism,who underwent liver transplantation but also significantly enhanced the perioperative liver function.

Comparison of pre-treatment and post-treatment use of selenium in retinal ischemia reperfusion injury

AIM: To investigate the effects of selenium in rat retinal ischemia reperfusion(IR) model and compare pretreatment and post-treatment use.METHODS: Selenium pre-treatment group(n =8) was treated with intraperitoneal(i.p.) selenium 0.5 mg/kg for7 d and terminated 24 h after the IR injury. Selenium posttreatment group( n = 8) was treated with i. p. selenium0.5 mg/kg for 7d after the IR injury with termination at the end of the 7d period. Sham group(n =8) received i.p.saline injections identical to the selenium volume for 7d with termination 24 h after the IR injury. Control group(n =8) received no intervention. Main outcome measures were retina superoxide dismutase(SOD), glutathione(GSH),total antioxidant status(TAS), malondialdehyde(MDA),DNA fragmentation levels, and immunohistological apoptosis evaluation.RESULTS: Compared to the Sham group, selenium pre-treatment had a statistical difference in all parameters except SOD. Post-treatment selenium also resulted in statistical differences in all parameters except the MDA levels. When comparing selenium groups, the pre-treatment selenium group had a statistically higher success in reduction of markers of cell damage such as MDA and DNA fragmentation. In contrast, the post-selenium treatment group had resulted in statisticallyhigher levels of GSH. Histologically both selenium groups succeeded to limit retinal thickening and apoptosis. Pre-treatment use was statistically more successful in decreasing apoptosis in ganglion cell layer compared to post-treatment use.CONCLUSION: Selenium was successful in retinal protection in IR injuries. Pre-treatment efficacy was superior in terms of prevention of tissue damage and apoptosis.

Effect of microwave pre-treatment on physical quality,bioactive compounds,safety risk factor,and storage stability of peanut butter

Microwave,as a new heat treatment technology,has the characteristics of uniform and fast heating speed.It is an energy-saving technology known for improving oilseed product quality.Its efficiency mainly depends on the roasting power and time.However,the production of high-quality peanut butter using short-time roasting con-ditions are limited.Herein,we determined an appropriate microwave roasting power and time for peanuts and evaluated its impacts on the quality of peanut butter.Different roasting powers(400 W,800 W and 1200 W)and times(4,4.5,5,and 5.5 min)were preliminarily tested.Among them,800 W at 5 min was the most suitable.The roasting efficiency was further evaluated using color,sensory,bioactive compounds,storage stability,and safety risk factors of peanut butter produced from four peanut cultivars(Silihong,Baisha-1016,Yuanza-9102,and Yuhua-9414).The pre-treated butter obtained from three cultivars(Silihong,Yuanza-9102,and Yuhua-9414)with moisture content between 5%and 7.2%had a similar sensory score(6-7)as the commercial on a 9-point hedonic scale compare to the other.The color of the pre-treated peanut butter varies statistically with the commercial but remained in the recommended range of Hunter L*values of 51-52,respectively,for high initial moisture peanut cultivars.The total polyphenol(35.20-31.59±0.59μmol GAE/g)and tocopherol(19.05±0.35 mg/100 g)content in the butter obtained from three cultivars(Yuahua-9102,Yuhua,and Baisha-1016)and Silihong respectively,were significantly(P<0.05)higher than those in the commercial butter.The induction times of all pre-treated butter(19.80±0.99-7.84±0.07 h)were significantly(P<0.05)longer during storage at accelerated temperature than commercial samples.In addition,no benzo[a]pyrene was found in the pre-treated samples.Collectively,the microwave pretreated peanut butter was superior to the commercial one.These findings provided data support and a reference basis to promote microwave use for peanut butter production.

Tree-ring δ^(15)N of Qinghai spruce in the central Qilian Mountains of China:Is pre-treatment of wood samples necessary?

A knowledge of the tree-ring stable nitrogen isotope ratio(δ^(15)N)can deepen our understanding of forest ecosystem dynamics by indicating the long-term availability,cycling and sources of nitrogen(N).However,the radial mobility of N blurs the interannual variations in the long-term N records.Previous studies of the chemical extraction of tree rings before analysis had produced inconsistent results and it is still unclear whether it is necessary to pre-treat wood samples from specific tree species to remove soluble N compounds before determining theδ^(15)N values.We compared the effects of pre-treatment with organic solvents and hot ultrapure water on the N concentration andδ^(15)N of tree rings from endemic Qinghai spruce(Picea crassifolia)growing in the interior of the central Qilian Mountains,China,during the last 60 a.We assessed the effects of different preparation protocols on the removal of the labile N compounds and investigated the need to pre-treat wood samples before determining theδ^(15)N values of tree rings.Increasing trends of the tree-ring N concentration were consistently observed in both the extracted and unextracted wood samples.The total N removed by extraction with organic solvents was about 17.60%,with a significantly higher amount in the sapwood section(P<0.01).Theδ^(15)N values of tree rings decreased consistently from 1960 to 2019 in both the extracted and unextracted wood samples.Extraction with organic solvents increased theδ^(15)N values markedly by about 5.2‰and reduced the variations in theδ^(15)N series.However,extraction with hot ultrapure water had little effect,with only a slight decrease in theδ^(15)N values of about 0.5‰.Our results showed that the radial pattern in the inter-ring movement of N in Qinghai spruce was not minimized by extraction with either organic solvents or hot ultrapure water.It is unnecessary to conduct hot ultrapure water extraction for the wood samples from Qinghai spruce because of its negligible effect on the removal of the labile N.Theδ^(15)N variation trend of tree rings in the unextracted wood samples was not influenced by the heartwood-sapwood transition zone.We suggest that theδ^(15)N values of the unextracted wood samples of the climate-sensitive Qinghai spruce could be used to explore the ecophysiological dynamics while focusing on the long-term variations.

数智化全自动标本转移系统在手术病理标本前处理流程中的应用

目的:探讨在数智化管理背景下,由手术室护士主导的数智化全自动标本转移系统在手术病理标本前处理流程中的应用效果,为提高手术病理标本质量提供参考。方法:构建数智化全自动标本转移系统,并将其应用于手术病理标本前处理流程中,比较系统应用前(2023年4月—7月)和系统应用后(2023年8月—11月)的病理标本送检效率、送检质量、手术室护士心理压力及医护人员满意度。结果:应用数智化全自动标本转移系统后,病理标本前处理流程时长较应用前平均减少3.66 min,一次性接收合格率由87.07%提高至96.42%,病理送检质量满意率较高,护士工作压力显著缓解,医务人员满意度明显提高,以上差异均具有统计学意义(P<0.05)。结论:数智化全自动标本转移系统应用于手术标本前处理流程中可有效提高手术标本前处理效率并缓解护士工作压力,保障病理标本送检质量,为医疗诊断提供可靠的依据,有效减少医务人员职业暴露,提升医护人员满意度。

贮前延迟联合热水处理对黄瓜果实冷藏期间冷害和生理代谢的影响

为探究黄瓜果实经热水处理后延迟方式对其冷藏期间品质和生理代谢的影响,根据前期利用二次正交旋转组合试验设计优化得出的延迟条件,以新鲜黄瓜为对照(CK),分别研究了水浴延迟(T1)、空气延迟(T2)和未延迟处理(T3)对经热激处理的黄瓜果实在(4±1)℃,相对湿度(80±5)%的冷库贮藏期间冷害(chilling injury,CI)症状、品质及抗氧化系统的影响。结果表明,低温贮藏3 d时,各组冷害指数最高仅为0.37,然而贮藏9 d时,CK和T3组果实冷害指数迅速升高至0.76和0.71,此时CK组冷害指数为T1和T2的1.25倍和1.21倍。而且T1和T2组能显著减缓果实失重率和电解质外渗率(electrolyte leakage,EL)的上升以及硬度的下降,同时能使果实保持较好色泽,维持较高的可溶性蛋白含量,有效减轻了丙二醛(malondialdehyde,MDA)、超氧阴离子(superoxide anion,O_(2)^(-)·)和过氧化氢(hydrogen peroxide,H_(2)O_(2))对果实的损伤;到贮藏末期,相比于CK,T1和T2组果实的H_(2)O_(2)含量分别降低了7.86%和11.64%。此外,T1和T2处理也能在一定程度上延缓过氧化氢酶(catalase,CAT)、过氧化物酶(peroxidase,POD)和还原型谷胱甘肽(reducedglutathione,GSH)含量的下降。综合分析,T1和T2处理组对于减轻黄瓜果实冷害,缓解品质劣变均有显著影响,可根据实际情况灵活选择适用性更高的处理方式。该研究对黄瓜贮藏保鲜技术的进一步完善有一定的理论和实践参考价值。

预检-挂号一体化信息系统在改进急诊科就诊流程及提升患者就诊体验中的作用分析

目的探讨分析预检-挂号一体化信息系统在改进急诊科就诊流程及提升患者就诊体验中的作用。方法选取2022年1月至12月浙江大学医学院附属儿童医院收治的100例急诊患者,为对照组,实施传统就诊模式,即预检与挂号分开进行;选取2023年1月至12月医院收治的100例急诊患者,为观察组,实施预检-挂号一体化就诊模式。对比两组患者分诊时间、分诊准确率、候诊时间、医务人员满意度及患者满意度。结果观察组分诊时间短于对照组,分诊准确率高于对照组,差异有统计学意义(P<0.05)。观察组总候诊时间较对照组短,差异有统计学意义(P<0.05)。观察组医护人员各条目及总分满意度评分均高于对照组,且差异有统计学意义(P<0.05)。观察组患者各条目评分及满意度总分均高于对照组,差异有统计学意义(P<0.05)。结论预检-挂号一体化信息系统可改进急诊科就诊流程,缩短分诊及候诊时间,提高分诊准确率,并可有效提升医务人员及患者的就诊满意度。

Design and Research of High-throughput Automation System for Food Testing Laboratory

In the process of food testing,human operation is an important variable affecting the experimental results.In order to reasonably avoid the influence of human subjective operation behavior on the accuracy of detection results,the laboratory information management system was used as the information platform to design a high-throughput laboratory automation pre-treatment system based on the deep integration of mechanical principles,visual analysis,high-speed conduction,intelligent storage and other technical systems.The experimental results showed that the system could shorten the sample circulation cycle,effectively improve the laboratory biosafety,and meet the requirements of high-throughput processing of samples.

Potential role of Lycium barbarum polysaccharides in glaucoma management:evidence from preclinical in vivo studies

In recent years,the pharmacological benefits of herbal extracts have been revisited for their potential neuroprotective effects in glaucoma.The polysaccharides extracted from the fruits of Lycium barbarum L.,or Lycium barbarum polysaccharides,exert their anti-aging effect through reducing oxidative stress,modulating the immune response,enhancing neuronal responses,and promoting cytoprotection.The therapeutic efficacy of Lycium barbarum polysaccharides in preserving retinal ganglion cells and their functions was demonstrated in a range of experimental models of optic neuropathies.These include the acute and chronic ocular hypertension models,the partial optic nerve transection model,and the ischemic-reperfusion injuries model.Based on these findings,Lycium barbarum polysaccharides appear to be a good candidate to be developed as a neuroprotective agent for treating multifactorial diseases.This review aims to present a comprehensive review on the latest preclinical evidence on the pre-and post-treatment benefits of Lycium barbarum polysaccharides in retinal ganglion cell neuroprotection.The possible mechanisms of Lycium barbarum polysaccharides mediating retinal ganglion cell neuroprotection will also be described.Moreover,the potential research gaps in the effective translation of Lycium barbarum polysaccharides treatment into clinical glaucoma management will be discussed.

5G互联转运系统研发及应用实践

目的:提升急救效率,探索院前-院内急救转运医疗服务新模式。方法:利用5G网络对原有重症救护车进行改造,在救护车内装载5G脉搏一号,基于用户面功能锚点(UPF)对5G网络进行弹性切片,实现与转运系统的稳定高效互联。结果:在物理终端层实现了心电、床旁监护仪参数快速数据采集,网络传输层实现了数据传输到院内急诊5G抢救室,并同步到医院5G指挥中心。结论:5G互联转运系统提升了院前急救的时效性,实现了院前-院内急诊救治一体化。

Effect of Pre-Sowing Treatments on Mamalis (Pittosporum pentandrum Blanco Merr.) Seeds Germination under Nursery Condition

Seeds were subjected to three different pre-sowing seed treatments: immersion in lukewarm water for 2 hours, immersion in concentrated sulfuric acid (H2SO4) for 5 minutes, hilar removal, and a control in which the seeds were sown without being treated. The experiment was laid out in the Completely Randomized Design (CRD) with four replicates and 60 seeds per treatment. Seeds were sown in an improvised Seedbox in October 2019. Germination was monitored daily for one month. The results showed that Mamalis seeds treated with lukewarm water have the earliest germination of twelve days, with a germination percentage of 66.67%. The germination rate of another treatment ranges from 0 - 44 percent, compared to 45 percent for the control treatment. It seems prudent to conclude that to enhance the vegetative propagation methods is to soaking in warm water at 37.5˚C for 2 hours could provide the best growth.

院前急救与院内救治智能手术网络系统在创伤患者救治中的应用效果观察

目的 探讨院前急救与院内救治智能手术网络系统(TPEITS)在创伤患者救治中的应用效果。方法 106例创伤患者根据干预方案的不同分为两组,对照组采用常规院前急救及院内救治,观察组采用院前急救与TPEITS,比较两组的抢救效果、并发症、满意度。结果 观察组的有效救治时间、住院时间均短于对照组,并发症发生率低于对照组,满意度高于对照组(P<0.05)。结论 院前急救与TPEITS能够缩短创伤患者的有效救治时间及住院时间,降低并发症发生率,提高满意度。

社区联动下胸痛患者院前救治体系的实践与思考

对危重胸痛病人的救治,时间就是生命。上海交通大学附属胸科医院与长宁区、市急救中心紧密合作,从急救120、社区医生、胸痛中心、居民等维度,通过流程标准化、工作信息化、救治协同化、宣教社区化建设,建立了"社区—胸科"一体化的社区联动救治模式,缩短了从病人发生胸痛或前兆到首次医疗接触的时间,提升了救治效果。建议要进一步优化胸痛中心布局,减少基层转诊延误时间,优化信息化快速反应力,提升自我保健判断力。

Fluent与Patran的接口实现及其在船舶力学中的应用

结合当前Fluent和Patran软件的特点,并针对船舶力学计算、流固耦合分析等的需要,阐述了两者接口开发的重要性。还对Fluent和Patran接口的功能、开发过程做了详细的介绍,并给出了应用的算例说明,对接口在船舶力学中的应用做了有启发性的展望。

活性污泥中温厌氧消化热水解预处理工艺及系统

从污泥热水解的机理出发,总结了热水解对污泥的作用过程及其对污泥特性的影响。在阐述热水解对污泥厌氧消化的强化作用及其强化机理的基础上,详细介绍了由同济大学开发的热水解污泥预处理系统及其作用机理和处理效率。

高浓度香料香精废水处理工程设计

香料香精废水中含有苯、酚、醇、胺、醛类及脂类、油类等物质,硫酸盐含量高,水量、水质变化大。设计采用隔油—气浮—微电解—催化氧化预处理工艺,UASB—两级A/O生化工艺,混凝/沉淀—砂滤深度处理工艺。运行结果表明:当高浓度原水CODCr高达18 073 mg/L时,出水CODCr仍在100 mg/L以下,其他指标均达到《污水综合排放标准》(GB 8978—1996)一级标准。

磁力机械式氧分析仪预处理系统的改进

在监测焦炉煤气氧含量的磁力机械式氧分析仪系统中,采样预处理系统是一个重要环节。在介绍磁力机械式氧分析仪的工作原理和采样预处理系统结构的基础上,结合现场实际,分析了采样预处理系统存在的问题,提出了相应的改进措施,经实施后收到了良好的效果,分析仪运行稳定、测量准确,过滤纸和分析仪测量室的寿命延长。

热力发电厂水汽氢电导率超标原因分析及处理

氢电导率超标,可能影响锅炉的水化学工况,导致锅炉受热面腐蚀甚至引发爆管事故。通过对某发电厂11、12号机组水汽系统氢电导率长期超标问题进行分析研究,找出了氢电导率超标的原因。对预处理系统进行改造,减少丙酮肟的加药量,大大提升了氢电导率的合格率。

UASB工艺系统设计方法探讨

本文全面介绍了UASB系统的设计问题。重点介绍了厌氧预处理工艺和UASB反应器的负荷设计原则和设计方法、混凝土结构矩形UASB反应器各个部分尺寸的计算和确定原则。对UASB的进水配水系统和布水方式作了详细介绍。对三相分离器和UASB建筑材料等问题也进行了讨论。

电厂化学水处理DCS的应用研究

国电谏壁发电厂为全面提升水汽处理的自动化水平,提高水汽品质,满足热力设备长期稳定、安全、经济运行的需要,进行了300MW机组化学水处理分散控制系统(DCS)的应用研究。将DCS应用于电厂化学水处理系统,解决了一些目前同类程控系统难以解决的问题。在项目实施过程中针对国电谏壁发电厂化学水处理DCS应用的技术难点,充分发挥了DCS固有的技术优势,实现了锅炉内、外水汽品质的全面、集中、综合控制。化学水处理DCS的应用,拓展了控制范围,实现了较复杂的控制策略,对实现电厂DCS管控一体化有借鉴作用。