Objective:Some patients exhibit septic symptoms following laparoscopic surgery,leading to a poor prognosis.Effective clinical subphenotyping is critical for guiding tailored therapeutic strategies in these cases.By id...Objective:Some patients exhibit septic symptoms following laparoscopic surgery,leading to a poor prognosis.Effective clinical subphenotyping is critical for guiding tailored therapeutic strategies in these cases.By identifying predisposing factors for postoperative sepsis,clinicians can implement targeted interventions,potentially improving outcomes.This study outlines a workflow for the subphenotype methodology in the context of laparoscopic surgery,along with its practical application.Methods:This study utilized data routinely available in clinical case systems,enhancing the applicability of our findings.The data included vital signs,such as respiratory rate,and laboratory measures,such as blood sodium levels.The process of categorizing clinical routine data involved technical complexities.A correlation heatmap was used to visually depict the relationships between variables.Ordering points were used to identify the clustering structure and combined with Consensus K clustering methods to determine the optimal categorization.Results:Our study highlighted the intricacies of identifying clinical subphenotypes following laparoscopic surgery,and could thus serve as a valuable resource for clinicians and researchers seeking to explore disease heterogeneity in clinical settings.By simplifying complex methodologies,we aimed to bridge the gap between technical expertise and clinical application,fostering an environment where professional medical knowledge is effectively utilized in subphenotyping research.Conclusion:This tutorial could primarily serve as a guide for beginners.A variety of clustering approaches were explored,and each step in the process contributed to a comprehensive understanding of clinical subphenotypes.展开更多
Precision surgery for primary liver cancer consists of precise exposure of hepatic veins and portal pedicles.If you resect a portion of the segment or subsegment of Couinaud perfectly,it is quite natural,that the hepa...Precision surgery for primary liver cancer consists of precise exposure of hepatic veins and portal pedicles.If you resect a portion of the segment or subsegment of Couinaud perfectly,it is quite natural,that the hepatic veins which run on the intersegmental plane or intersubsegmental plane should be exposed and the portal pedicle.展开更多
Gastrointestinal(GI)cancer has a high tumor incidence and mortality rate worldwide.Despite significant improvements in radiotherapy,chemotherapy,and targeted therapy for GI cancer over the last decade,GI cancer is cha...Gastrointestinal(GI)cancer has a high tumor incidence and mortality rate worldwide.Despite significant improvements in radiotherapy,chemotherapy,and targeted therapy for GI cancer over the last decade,GI cancer is characterized by high recurrence rates and a dismal prognosis.There is an urgent need for new diagnostic and therapeutic approaches.Recent technological advances and the accumulation of clinical data are moving toward the use of precision medicine in GI cancer.Here we review the application and status of precision medicine in GI cancer.Analyses of liquid biopsy specimens provide comprehensive real-time data of the tumor-associated changes in an individual GI cancer patient with malignancy.With the introduction of gene panels including next-generation sequencing,it has become possible to identify a variety of mutations and genetic biomarkers in GI cancer.Although the genomic aberration of GI cancer is apparently less actionable compared to other solid tumors,novel informative analyses derived from comprehensive gene profiling may lead to the discovery of precise molecular targeted drugs.These progressions will make it feasible to incorporate clinical,genome-based,and phenotype-based diagnostic and therapeutic approaches and apply them to individual GI cancer patients for precision medicine.展开更多
What if doctors could precisely edit your DNA to correct disease-causing mutations?Thanks to rapid advances in gene editing tools,this futuristic concept is creeping closer to reality.But precise genomic“surgery”rem...What if doctors could precisely edit your DNA to correct disease-causing mutations?Thanks to rapid advances in gene editing tools,this futuristic concept is creeping closer to reality.But precise genomic“surgery”remains challenging.Now,scientists have developed an innovative technique to integrate new DNA sequences into cells with record efficiency and accuracy-an important step toward next-generation gene therapies.展开更多
背景:目前用于全膝关节置换的机器人系统设计的基本原理是将三维手术规划、术中危险区预警、实时数据反馈及机械臂辅助截骨等技术相结合,以实现全膝关节置换的精准化、个性化,这也恰好是它最大优势所在,因此近年来成为关节外科领域热点...背景:目前用于全膝关节置换的机器人系统设计的基本原理是将三维手术规划、术中危险区预警、实时数据反馈及机械臂辅助截骨等技术相结合,以实现全膝关节置换的精准化、个性化,这也恰好是它最大优势所在,因此近年来成为关节外科领域热点话题,备受关注。目的:文章将从机器人辅助全膝关节置换在关节外科领域的发展现状及其与传统全膝关节置换优劣势对比进行概述,此外,还将对机器人辅助全膝关节置换技术未来的发展进行展望。方法:应用计算机检索PubMed、中国知网、万方和维普数据库的相关文章,英文检索词:“robot OR robotic OR robotics OR robotically OR computer,total knee arthroplasty OR total knee replacement,TKA OR TKR”,中文检索词:“机器人辅助,计算机导航,全膝关节置换术”,最终纳入64篇文献进行综述分析。结果与结论:①用于辅助全膝关节置换的机器人系统根据其自由度分为主动式、半主动式和被动式。半主动式系统是目前使用最为广泛的机器人系统,该系统有效提高了全膝关节置换手术的精准性和个性化程度,但其高昂的使用成本与较长的学习曲线仍是在该领域内推广时需要权衡的主要因素。②机器人辅助全膝关节置换可实现膝关节局部三维空间的精准截骨、正确安置假体,已被广泛证明可以提供更好的假体植入精准度,减少影像学异常值,在术中可获得良好的软组织平衡,最终改善术后膝关节运动及功能状态。③但目前的机器人辅助系统依然存在客观的不足之处,包括不同机器人设备与术者之间的学习曲线问题、额外增加的安装和维护成本以及与机器人手术相关的潜在并发症,所以其能否让医疗系统及患者真正受益仍需要更长期的研究予以证明,机器人辅助系统也仍需进行更多实质性的改进。④机器人辅助全膝关节置换技术在临床上仍然处于初步研究阶段,并没有大范围地应用到临床,更加明确该技术的用法、完善该技术的临床操作规范和安全性成为了未来对该技术的研究侧重点。展开更多
目的:探讨精准营养联合情绪释放干预对腹腔镜结直肠癌根治术患者营养水平、免疫功能及心理韧性的影响。方法:选取2021年6月—2022年12月于宜春市人民医院进行腹腔镜结直肠癌根治术的患者64例,根据随机数字表法分为两组。对照组(n=32)采...目的:探讨精准营养联合情绪释放干预对腹腔镜结直肠癌根治术患者营养水平、免疫功能及心理韧性的影响。方法:选取2021年6月—2022年12月于宜春市人民医院进行腹腔镜结直肠癌根治术的患者64例,根据随机数字表法分为两组。对照组(n=32)采用常规肠外营养疗法及情绪释放干预,研究组(n=32)采用精准营养疗法联合情绪释放干预。对两组营养水平、免疫功能及心理韧性进行对比及分析。结果:干预后研究组血红蛋白(hemoglobin,Hb)、白蛋白(albumin,ALB)、血清总蛋白(serum total protein,STP)、转铁蛋白(transferrin,TRF)水平均较对照组更高(P<0.05)。干预后研究组CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)均较对照组更高(P<0.05)。干预后研究组免疫球蛋白(immunoglobulin,Ig)A、IgG、IgM水平均较对照组更高(P<0.05)。干预后研究组坚韧、乐观、力量评分及总分均较对照组更高(P<0.05)。结论:精准营养疗法联合情绪释放干预能有效增强腹腔镜结直肠癌根治术患者营养状况、免疫功能及心理韧性。展开更多
基金The study was funded by the China National Key Research and Development Program(2022YFC2504503,2023YFC3603104)General Health Science and Technology Program of Zhejiang Province(2024KY1099)+2 种基金the Huadong Medicine Joint Funds of the Zhejiang Provincial Natural Science Foundation of China(LHDMD24H150001)National Natural Science Foundation of China(82272180)the Project of Drug Clinical Evaluate Research of Chinese Pharmaceutical Association(CPA-Z06-ZC-2021e004).
文摘Objective:Some patients exhibit septic symptoms following laparoscopic surgery,leading to a poor prognosis.Effective clinical subphenotyping is critical for guiding tailored therapeutic strategies in these cases.By identifying predisposing factors for postoperative sepsis,clinicians can implement targeted interventions,potentially improving outcomes.This study outlines a workflow for the subphenotype methodology in the context of laparoscopic surgery,along with its practical application.Methods:This study utilized data routinely available in clinical case systems,enhancing the applicability of our findings.The data included vital signs,such as respiratory rate,and laboratory measures,such as blood sodium levels.The process of categorizing clinical routine data involved technical complexities.A correlation heatmap was used to visually depict the relationships between variables.Ordering points were used to identify the clustering structure and combined with Consensus K clustering methods to determine the optimal categorization.Results:Our study highlighted the intricacies of identifying clinical subphenotypes following laparoscopic surgery,and could thus serve as a valuable resource for clinicians and researchers seeking to explore disease heterogeneity in clinical settings.By simplifying complex methodologies,we aimed to bridge the gap between technical expertise and clinical application,fostering an environment where professional medical knowledge is effectively utilized in subphenotyping research.Conclusion:This tutorial could primarily serve as a guide for beginners.A variety of clustering approaches were explored,and each step in the process contributed to a comprehensive understanding of clinical subphenotypes.
文摘Precision surgery for primary liver cancer consists of precise exposure of hepatic veins and portal pedicles.If you resect a portion of the segment or subsegment of Couinaud perfectly,it is quite natural,that the hepatic veins which run on the intersegmental plane or intersubsegmental plane should be exposed and the portal pedicle.
基金Supported by KAKENHI(Grant-in-Aid for Scientific Research),No.18H02883
文摘Gastrointestinal(GI)cancer has a high tumor incidence and mortality rate worldwide.Despite significant improvements in radiotherapy,chemotherapy,and targeted therapy for GI cancer over the last decade,GI cancer is characterized by high recurrence rates and a dismal prognosis.There is an urgent need for new diagnostic and therapeutic approaches.Recent technological advances and the accumulation of clinical data are moving toward the use of precision medicine in GI cancer.Here we review the application and status of precision medicine in GI cancer.Analyses of liquid biopsy specimens provide comprehensive real-time data of the tumor-associated changes in an individual GI cancer patient with malignancy.With the introduction of gene panels including next-generation sequencing,it has become possible to identify a variety of mutations and genetic biomarkers in GI cancer.Although the genomic aberration of GI cancer is apparently less actionable compared to other solid tumors,novel informative analyses derived from comprehensive gene profiling may lead to the discovery of precise molecular targeted drugs.These progressions will make it feasible to incorporate clinical,genome-based,and phenotype-based diagnostic and therapeutic approaches and apply them to individual GI cancer patients for precision medicine.
文摘What if doctors could precisely edit your DNA to correct disease-causing mutations?Thanks to rapid advances in gene editing tools,this futuristic concept is creeping closer to reality.But precise genomic“surgery”remains challenging.Now,scientists have developed an innovative technique to integrate new DNA sequences into cells with record efficiency and accuracy-an important step toward next-generation gene therapies.
文摘背景:目前用于全膝关节置换的机器人系统设计的基本原理是将三维手术规划、术中危险区预警、实时数据反馈及机械臂辅助截骨等技术相结合,以实现全膝关节置换的精准化、个性化,这也恰好是它最大优势所在,因此近年来成为关节外科领域热点话题,备受关注。目的:文章将从机器人辅助全膝关节置换在关节外科领域的发展现状及其与传统全膝关节置换优劣势对比进行概述,此外,还将对机器人辅助全膝关节置换技术未来的发展进行展望。方法:应用计算机检索PubMed、中国知网、万方和维普数据库的相关文章,英文检索词:“robot OR robotic OR robotics OR robotically OR computer,total knee arthroplasty OR total knee replacement,TKA OR TKR”,中文检索词:“机器人辅助,计算机导航,全膝关节置换术”,最终纳入64篇文献进行综述分析。结果与结论:①用于辅助全膝关节置换的机器人系统根据其自由度分为主动式、半主动式和被动式。半主动式系统是目前使用最为广泛的机器人系统,该系统有效提高了全膝关节置换手术的精准性和个性化程度,但其高昂的使用成本与较长的学习曲线仍是在该领域内推广时需要权衡的主要因素。②机器人辅助全膝关节置换可实现膝关节局部三维空间的精准截骨、正确安置假体,已被广泛证明可以提供更好的假体植入精准度,减少影像学异常值,在术中可获得良好的软组织平衡,最终改善术后膝关节运动及功能状态。③但目前的机器人辅助系统依然存在客观的不足之处,包括不同机器人设备与术者之间的学习曲线问题、额外增加的安装和维护成本以及与机器人手术相关的潜在并发症,所以其能否让医疗系统及患者真正受益仍需要更长期的研究予以证明,机器人辅助系统也仍需进行更多实质性的改进。④机器人辅助全膝关节置换技术在临床上仍然处于初步研究阶段,并没有大范围地应用到临床,更加明确该技术的用法、完善该技术的临床操作规范和安全性成为了未来对该技术的研究侧重点。
文摘目的:探讨精准营养联合情绪释放干预对腹腔镜结直肠癌根治术患者营养水平、免疫功能及心理韧性的影响。方法:选取2021年6月—2022年12月于宜春市人民医院进行腹腔镜结直肠癌根治术的患者64例,根据随机数字表法分为两组。对照组(n=32)采用常规肠外营养疗法及情绪释放干预,研究组(n=32)采用精准营养疗法联合情绪释放干预。对两组营养水平、免疫功能及心理韧性进行对比及分析。结果:干预后研究组血红蛋白(hemoglobin,Hb)、白蛋白(albumin,ALB)、血清总蛋白(serum total protein,STP)、转铁蛋白(transferrin,TRF)水平均较对照组更高(P<0.05)。干预后研究组CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)均较对照组更高(P<0.05)。干预后研究组免疫球蛋白(immunoglobulin,Ig)A、IgG、IgM水平均较对照组更高(P<0.05)。干预后研究组坚韧、乐观、力量评分及总分均较对照组更高(P<0.05)。结论:精准营养疗法联合情绪释放干预能有效增强腹腔镜结直肠癌根治术患者营养状况、免疫功能及心理韧性。
