Impact of three different processing methods on the digestibility and allergenicity of Chinese mitten crab(Eriocheir sinensis)tropomyosin

The tropomyosin(TM)fractions of crab protems may cause allergic reactions in mdividuals susceptible to allergies;however,efficient and safe methods by which to reduce such allergenicity are not currently available.Therefore,in this study,the effects of three different processing methods,i.e.,microwave,ultrasound,and high temperature-pressure(HTP)treatments,on the digestion stability of TM from Chinese mitten crab muscle and the allergenicity of TM digestion products were explored.sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that microwaving had little effect on the digestion stability of TM.In contrast,ultrasound and HTP treatments facilitated the degradation of TM.Similarly,Western blotting and inhibition ELISA indicated that the IgE-binding activity of TM was significantly reduced after treatment with ultrasound or HTP.Among the three different proces sing methods,HTP was the most effective method for improving digestibility of TM and reducing immunoreactivity.This finding provides new insights into treatments for crab allergies.

Impact of Selected Processing Methods of High-Level Cyanide in Cassava on Optic Neuropathy in Wistar Albino Rats—An Experimental Study

Background: Cassava tuber crop is a staple food rich in carbohydrates and utilized in various forms by millions of Nigerians. The storage root of the cassava contains linamarin, a cyanogenic glycoside that is easily hydrolyzed to release cyanide salt compounds which is toxic to the nervous system especially the optic nerve, sometimes leading to optic neuropathy and visual impairment. Aim: The aim of this study is to find out the impact of selected processing methods of high-level cyanide in cassava on optic neuropathy in Wistar albino rats. Methodology: Twenty-four Wistar albino rats were fed with different concentration and duration of predetermined high-cyanide content cassava root cultivar which was processed using different processing methods adopted by various communities in Rivers State, Nigeria (for human consumption). A control group of 3 Wistar albino rats was fed with normal “Growth Mesh” meals. The pre and post weights of the animals and the fundoscopic optic nerve status of the rats were evaluated after 30 and 60 days. SPSS Version 25 was employed for descriptive and inferential statistical analyses. A p-value of ≤0.05 was considered statistically significant. Results: The Cassava species available in Rivers State have high cyanide content (2336.79 mg CN-/kg dry weight of cassava). There was statistically significant reduction in the cyanide content (p = 0.000) depending on the various common processing methods (into garri for human consumption): 24 hours, 48 hours, fermentation;with and without red palm oil additive. The individual weights as well as the mean weight of the 24 rats in the experimental group increased gradually from the first week to the 9th week with a slight weight reduction on the third and fourth weeks which was not statistically significant (p = 0.092). However, there was a steady increase in the weights of the animals in the control group throughout the 9 weeks. Varying degrees of optic neuropathy occurred, worse with the rats that had 24-hour fermented cassava twice daily for 60 days. The intra and inter group differences in the optic disc changes was statistically significant (p = 0.000). Conclusion: Longer duration of processing cassava roots into garri for human consumption reduces its cyanide content and minimizes the adverse impact on the optic nerve.

Influence of Different Processing Methods on Honeysuckle Quality

Processing method is one of the maln factors affecting the quality of hon-eysuckIe herbs, which is directIy reIated to economic benefits of farmers. This paper compares various processing methods of honeysuckIe to provide some references for deveIoping a suitabIe processing procedure that can be used in Iarge-scale pro-duction and improve herb quality.

Accumulation of L-DOPA in various organs of faba bean and influence of drought, nitrogen stress, and processing methods on L-DOPA yield

Faba bean(Vicia faba L.)has been identified as a rich source of L-DOPA,which is used in treating Parkinson's disease.Biosynthesis and accumulation of active substances such as L-DOPA in plant tissues may interact with growing conditions and processing methods.Accumulation trends of L-DOPA in various faba bean organs and the effect of drought stress and N fertilization on L-DOPA content were studied in a field and two greenhouse experiments.The influence of various processing methods on L-DOPA content of faba bean tissues was evaluated.The highest L-DOPA content was detected in fresh leaves(22.4 mg g^(-1))followed by flowers,young pods,mature seeds,and roots.Regardless of processing method,L-DOPA concentration in faba bean tissues was significantly reduced when tissues were boiled or dried.Among various methods of processing,freezing had the lowest detrimental effect,reducing L-DOPA concentrations by 24.1%and 21.1%in leaves and seeds,respectively.Drought stress elevated L-DOPA concentration,and maximum L-DOPA(23.3 mg g^(-1)of biomass)was extracted from plants grown under severe drought stress.However,L-DOPA yield(L-DOPA concentration×biomass)was compromised,owing to the adverse influence of drought stress on dry matter production.No significant difference in L-DOPA concentration was detected among various N application rates.

Effects of different processing methods on the lipid composition of hazelnut oil:a lipidomics analysis

Although hazelnut oil is rich in nutrients,its quality is greatly affected by how it is processed.However,no studies to date have comprehensively analyzed the lipid composition of hazelnut oil using different processing methods.Here,we conducted a lipidomics analysis using UPLC-QTOF-MS to characterize the lipid composition of cold-pressed hazelnut oil(CPO),ultrasonic-assisted hexane hazelnut oil(UHO)and enzyme-assisted aqueous hazelnut oil(EAO).A total of 10 subclasses of 98 lipids were identified,including35 glycerolipids(GLs),56 glycerophospholipids(GPs)and 7 sphingolipids(SPs).The total lipid and GL content were the highest in CPO,GP content was the highest in UHO and the ceramide content in SPs was most abundant in EAO.Multivariate statistical analysis showed that the lipid profiles of hazelnut oil prepared with different processing methods varied.Twelve significantly different lipids(TAG 54:3,TAG 52:2,TAG54:4,TAG 54:2,TAG 52:3,TAG 54:5,DAG 36:2,DAG 36:4,DAG 36:3,PC 36:2,PA 36:2 and PE 36:3)were identified,and these lipids could potentially be used as biomarkers to distinguish between hazelnut oil subjected to different processing methods.Our results provide useful information for hazelnut oil applications and new insight into the effects of edible oil processing.

Review of Processing Methods of Jujube

Historical evolution of jujube processing,recording situation of modern processing specifications of jujube,research status of processing methods of jujube at home in recent years,content analysis of main chemical composition in jujube processed products,and impact of processing on chemical composition of jujube are reviewed,which could provide basic data for fully studying,developing and utilizing jujube by scholars and technical staff.

Different processing methods and pharmacological effects of Atractylodis Rhizoma

Atractylodis Rhizoma,a traditional Chinese medicine with an extensive history of treating gastrointestinal disorders and other diseases,undergoes various processing methods in China to enhance its therapeutic efficacy for specific conditions.However,a comprehensive report detailing the changes in chemical composition and pharmacological effects due to these processing methods is currently lacking.This article provides a systematic review of the commonly employed processing techniques for Atractylodis Rhizoma,including raw Atractylodis Rhizoma(SCZ),bran-fried Atractylodis Rhizoma(FCZ),deep-fried Atractylodis Rhizoma(JCZ),and rice water-processed Atractylodis Rhizoma(MCZ).It examines the alterations in chemical constituents and pharmacological activities resulting from these processes and elucidates the mechanisms of action of the primary components in the various processed forms of Atractylodis Rhizoma in the treatment of gastrointestinal diseases.

信息型常见毒物质谱用户库的建立及其在毒物快速筛选上的应用研究

The spectrum user’s librarys containing 1533 familiar toxicants have been built; A method with high separability and sensitivity has been found,The RT and the detection limit of 210 familiar abuse drugs has been confirmed; the processing methods to analyse spectrum data automatically have been found; It is simple,quick,sensitive and reliable,and can take place of manual operation,It is very useful to screen the familiar toxicants in unkown sample.

Dietary energy sources and levels shift the multi-kingdom microbiota and functions in the rumen of lactating dairy cows

Background: Dietary energy source and level in lactation diets can profoundly affect milk yield and composition.Such dietary effects on lactation performance are underpinned by alteration of the rumen microbiota, of which bacteria, archaea, fungi, and protozoa may vary differently. However, few studies have examined all the four groups of rumen microbes. This study investigated the effect of both the level and source of dietary energy on rumen bacteria, archaea, fungi, and protozoa in the rumen of lactating dairy cows. A 2 × 2 factorial design resulted in four dietary treatments: low and high dietary energy levels(LE: 1.52–1.53;and HE: 1.71–1.72 Mcal/kg dry matter) and two dietary energy sources(GC: finely ground corn;and SFC: steam-flaked corn). We used a replicated 4 × 4 Latin square design using eight primiparous Chinese Holstein cows with each period lasting for 21 d. The rumen microbiota was analyzed using metataxonomics based on kingdom-specific phylogenetic markers [16 S r RNA gene for bacteria and archaea, 18 S r RNA gene for protozoa, and internally transcribed spacer 1(ITS1) for fungi] followed with subsequent functional prediction using PICRUSt2.Results: The GC resulted in a higher prokaryotic(bacterial and archaeal) species richness and Faith's phylogenetic diversity than SFC. For the eukaryotic(fungi and protozoa) microbiota, the LE diets led to significantly higher values of the above measurements than the HE diets. Among the major classified taxa, 23 genera across all the kingdoms differed in relative abundance between the two dietary energy levels, while only six genera(none being protozoal)were differentially abundant between the two energy sources. Based on prokaryotic amplicon sequence variants(ASVs) from all the samples, overall functional profiles predicted using PICRUSt2 differed significantly between LE and HE but not between the two energy sources. Fish Taco analysis identified Ruminococcus and Coprococcus as the taxa potentially contributing to the enriched KEGG pathways for biosynthesis of amino acids and to the metabolisms of pyruvate, glycerophospholipid, and nicotinate and nicotinamide in the rumen of HE-fed cows. The co-occurrence networks were also affected by the dietary treatments, especially the LE and GC diets, resulting in distinct co-occurrence networks. Several microbial genera appeared to be strongly correlated with one or more lactation traits.Conclusions: Dietary energy level affected the overall rumen multi-kingdom microbiota while little difference was noted between ground corn and steam-flaked corn. Some genera were also affected differently by the four dietary treatments, including genera that had been shown to be correlated with lactation performance or feed efficiency.The co-occurrence patterns among the genera exclusively found for each dietary treatment may suggest possible metabolic interactions specifically affected by the dietary treatment. Some of the major taxa were positively correlated to milk properties and may potentially serve as biomarkers of one or more lactation traits.

Effects of processing technology on essential oil and pharmacological action of frankincense

Background:According to the traditional Chinese medicine theory,the common processing methods for Ruxiang(frankincense)include stir-frying processing and vinegar processing.Methods:With network pharmacology and thermal analysis methods,we selected and identified the main and irritating ingredients,established the pyrolysis characteristic parameters for identifying frankincense quality,quantified the starting and optimum temperature limits for processing frankincense,and analyzed the influences of processing adjuvants(vinegar)on combustion pyrolysis characteristics of frankincense.By applying Fourier transformation infrared spectrometry,high performance liquid chromatography,gas chromatography and UV-visible spectrometry,we evaluated the processing technology developed in our study of frankincense and its processed products.Results:Based on network pharmacology,we can find that the classical compounds of essential oil are the main pharmacodynamics components of frankincense possessing the anti-inflammatory,analgesia,anti-tumor,anti-ulcer and ant-inflammatory bowel disease effects.The pyrolysis combustion rate peaks of frankincense at 285.27±7.05℃and 476.99±13.46℃were the characteristic peaks used to identify frankincense quality.The specific temperature of slow-fire processing,including the vinegar processing and stir-frying processing,was 148.72±5.09℃.Heating for 5.67±0.98 min after reaching processing temperature was needed for processing frankincense with vinegar.The kinetics analysis showed that both frankincense and its vinegar mixture exhibited good linear relationships.The feasibility and practicability for our processing technology were verified through the determination of medicinal compounds by Fourier transformation infrared spectrometry,high performance liquid chromatography,gas chromatography and UV.Conclusion:Frankincense essential oils were the main active components of frankincense,which also can be taken as the internal chemical control indexes to judge the quality control and to optimize the processing technology of frankincense.The pyrolysis combustion rate peaks of frankincense at 285.27±7.05℃and 476.99±13.46℃were the characteristic peaks that can be used to identify frankincense quality.The optimum processing conditions of vinegar-processed frankincense with were as follows:with vinegar,20%;heating temperature,148.72±5.09℃;and heating time,5.67±0.98 min.Under these conditions,frankincense essential oil could cause a moderate effect on combustion pyrolysis characteristics to exert its corresponding pharmacological effects.

Effects of Different Processing Modes of Reconstituted Tobacco on Cigarette Quality

In order to explore the most suitable mode for the primary processing model of reconstituted tobacco, another two kinds of conditioning process schemes for reconstituted tobacco were designed based on the existing reconstituted tobacco processing method. Comparative analysis and evaluation were conducted for moisture content uniformity, intrinsic chemical compositions, casing uniformity, blending uniformity and sensory quality of the samples from the three kind of processing modes. The results showed that loosing and conditioning or the vacuum conditioning pretreatment of the reconstituted tobacco could effectively reduce the difference in moisture content during the blending, and improve the casing uniformity and blending uniformity. Moreover, the samples with the loosing and conditioning pretreatment had higher total sugar and reducing sugar contents, which indicated that the loosing and conditioning pretreatment method was more favorable for the improvement of the intrinsic quality of cigarettes. In addition, the three kinds of aromatic components contents of cigarettes using the loosing and conditioning pretreatment were higher,and the casing uniformity, blending uniformity and overall sensory quality of this pretreatment were all superior to that of the other two kinds of reconstituted tobacco pretreatment methods. The contents of total sugar, reducing sugar as well as the three kinds of aromatic components of cigarettes processed with vacuum conditioning pretreatment were all slightly lower than those of the existing methods, but the casing uniformity and blending uniformity were improved, while it showed small difference in the sensory quality with the existing processing method.

An iterative wavefront sensing algorithm for high-contrast imaging systems

Wavefront sensing from multiple focal plane images is a promising technique for high-contrast imaging systems.However,the wavefront error of an optics system can be properly reconstructed only when it is very small.This paper presents an iterative optimization algorithm for the direct measurement of large static wavefront errors from only one focal plane image.We first measure the intensity of the pupil image to get the pupil function of the system and acquire the aberrated image on the focal plane with a phase error that will be measured.Then we induce a dynamic phase on the tested pupil function and calculate the associated intensity of the reconstructed image on the focal plane.The algorithm will then try to minimize the intensity difference between the reconstructed image and the aberrated test image in the focal plane,where the induced phase is a variable of the optimization algorithm.The simulation shows that the wavefront of an optical system can theoretically be reconstructed with high precision,which indicates that such an iterative algorithm may be an effective way to perform wavefront sensing for high-contrast imaging systems.

Alunite processing method selection using the AHP and TOPSIS approaches under fuzzy environment

Alunite is the most important non bauxite resource for alumina. Various methods have been proposed and patented for processing alunite, but none has been performed at industrial scale and no technical,operational and economic data is available to evaluate methods. In addition, selecting the right approach for alunite beneficiation, requires introducing a wide range of criteria and careful analysis of alternatives.In this research, after studying the existing processes, 13 methods were considered and evaluated by 14 technical, economic and environmental analyzing criteria. Due to multiplicity of processing methods and attributes, in this paper, Multi Attribute Decision Making methods were employed to examine the appropriateness of choices. The Delphi Analytical Hierarchy Process(DAHP) was used for weighting selection criteria and Fuzzy TOPSIS approach was used to determine the most profitable candidates. Among 13 studied methods, Spanish, Svoronos and Hazan methods were respectively recognized to be the best choices.

Improvements of Traditional Laser Fraunhofer Diffraction Experiment Using Digital Image Processing Method

We present an improved digital image processing(DIP)method to calculate the widths of single slits.Different from the traditional laser Fraunhofer diffraction experiment in college physical experiments,by performing fast Fourier transform,inverse fast Fourier transform and the nonlinear leastsquare fitting on the diffraction pattern taken by a camera,the DIP method can quickly return an analytic expression,whose period is used to calculate widths of single slits.By comparing the measured results by the DIP method and the successional difference(SD)method,we find that for a single slit whose width is 60372μm,the DIP method is more accurate.Experimental results show that for single slits with widths between 40μm and 160μm,the relative error of the DIP method is less than 2.78%.Also,the DIP method can be used to measure the diameter of filament and fibres online in real time.

Wavelet Analysis of Space Solar Telescope Images

The scientific satellite SST (Space Solar Telescope) is an important research project strongly supported by the Chinese Academy of Sciences. Every day, SST acquires 50 GB of data (after processing) but only 10GB can be transmitted to the ground because of limited time of satellite passage and limited channel volume. Therefore, the data must be compressed before transmission. Wavelets analysis is a new technique developed over the last 10 years, with great potential of application. We start with a brief introduction to the essential principles of wavelet analysis, and then describe the main idea of embedded zerotree wavelet coding, used for compressing the SST images. The results show that this coding is adequate for the job.

Data processing and initial results of Chang'e-3 lunar penetrating radar

To improve our understanding of the formation and evolution of the Moon, one of the payloads onboard the Chang＇e-3 （CE-3） rover is Lunar Penetrating Radar （LPR）. This investigation is the first attempt to explore the lunar subsurface structure by using ground penetrating radar with high resolution. We have probed the subsur- face to a depth of several hundred meters using LPR. In-orbit testing, data processing and the preliminary results are presented. These observations have revealed the con- figuration of regolith where the thickness of regolith varies from about 4 m to 6 m. In addition, one layer of lunar rock, which is about 330 m deep and might have been accumulated during the depositional hiatus of mare basalts, was detected.

Data processing and initial results from the CE-3 Extreme Ultraviolet Camera

The Extreme Ultraviolet Camera （EUVC） onboard the Chang＇e-3 （CE-3） lander is used to observe the structure and dynamics of Earth＇s plasmasphere from the Moon. By detecting the resonance line emission of helium ions （He＋） at 30.4 nm, the EUVC images the entire plasmasphere with a time resolution of 10 min and a spatial resolution of about 0.1 Earth radius （RE） in a single frame. We first present details about the data processing from EUVC and the data acquisition in the commissioning phase, and then report some initial results, which reflect the basic features of the plas- masphere well. The photon count and emission intensity of EUVC are consistent with previous observations and models, which indicate that the EUVC works normally and can provide high quality data for future studies.

A method and results of color calibration for the Chang'e-3 terrain camera and panoramic camera

The terrain camera （TCAM） and panoramic camera （PCAM） are two of the major scientific payloads installed on the lander and rover of the Chang＇e 3 mission re- spectively. They both use a Bayer color filter array covering CMOS sensor to capture color images of the Moon＇s surface. RGB values of the original images are related to these two kinds of cameras. There is an obvious color difference compared with human visual perception. This paper follows standards published by the International Commission on Illumination to establish a color correction model, designs the ground calibration experiment and obtains the color correction coefficient. The image qual- ity has been significantly improved and there is no obvious color difference in the corrected images. Ground experimental results show that： （1） Compared with uncor- rected images, the average color difference of TCAM is 4.30, which has been reduced by 62.1%. （2） The average color differences of the left and right cameras in PCAM are 4.14 and 4.16, which have been reduced by 68.3% and 67.6% respectively.

Stochastic parallel gradient descent based adaptive optics used for a high contrast imaging coronagraph

An adaptive optics （AO） system based on a stochastic parallel gradient descent （SPGD） algorithm is proposed to reduce the speckle noises in the optical system of a stellar coronagraph in order to further improve the contrast. The principle of the SPGD algorithm is described briefly and a metric suitable for point source imaging optimization is given. The feasibility and good performance of the SPGD algorithm is demonstrated by an experimental system featured with a 140-actuator deformable mirror and a Hartrnann-Shark wavefront sensor. Then the SPGD based AO is applied to a liquid crystal array （LCA） based coronagraph to improve the contrast. The LCA can modulate the incoming light to generate a pupil apodization mask of any pattern. A circular stepped pattern is used in our preliminary experiment and the image contrast shows improvement from 10^-3 to 10^-4.5 at an angular distance of 2A/D after being corrected by SPGD based AO.

Characterizing motion types of G-band bright points in the quiet Sun

We study the motion of G-band bright points （GBPs） in the quiet Sun to obtain the characteristics of different motion types. A high resolution image sequence taken with the Hinode/Solar Optical Telescope （SOT） is used, and GBPs are automat- ically tracked by segmenting 3D evolutional structures in a space-time cube. After putting the GBPs that do not move during their lifetimes aside, the non-stationary GBPs are categorized into three types based on an index of their motion type. Most GBPs that move in straight or nearly straight lines are categorized as a straight mo- tion type, a few moving in rotary paths into rotary motion, and the others fall into a motion type we called erratic. The mean horizontal velocities are 2.18±0.08 km s-1, 1.63±0.09km s^-1 and 1.33±0.07 km s^-1 for straight, erratic and rotary motion types, respectively. We find that a GBP drifts at a higher and constant velocity during its whole life if it moves in a straight line. However, it has a lower and variational velocity if it moves on a rotary path. The diffusive process is ballistic-, super- and sub-diffusion for straight, erratic and rotary motion types, respectively. The corresponding diffusion index （γ） and coefficients （K） are 2.13±0.09 and 850±37km^2 s^-1, 1.82±0.07 and 331 ±24 km^2 s^-1, and 0.73±0.19 and 13±9 km^2 s^-1. In terms of direction of motion, it is homogeneous and isotropic, and usually persists between neighboring frames, no matter what motion type a GBP is classified as.