利用InSAR监测矿山采空区地表沉降的过程中易在沉降中心周围产生失相干现象,导致形变结果缺失,无法获取准确的沉降区模型。基于此,提出了一种结合时序InSAR与PIM(Probability Integral Method)的监测方法,以云南省玉溪市大红山铁矿地下...利用InSAR监测矿山采空区地表沉降的过程中易在沉降中心周围产生失相干现象,导致形变结果缺失,无法获取准确的沉降区模型。基于此,提出了一种结合时序InSAR与PIM(Probability Integral Method)的监测方法,以云南省玉溪市大红山铁矿地下采区某工作面为研究对象,首先计算出时序InSAR累积沉降量,并提取其沉降区边缘的高相干点,结合少量沉降中心水准点,利用改进型PSO(Particle Swarm Optimization)算法获取PIM参数,并通过克里金插值法建立采空区地表整体沉降区模型,利用少量水准点数据对该方法的可靠性和精度进行验证,结果表明,该方法获得的沉降区分布与实际相吻合,其沉降区监测精度比单独使用时序InSAR或PIM的更高,可用于矿区地表沉降信息的准确获取。展开更多
BACKGROUND Resistance to sorafenib has become a challenge in clinical treatment of hepatocellular carcinoma(HCC).Physcion is a common bioactive anthraquinone that has potential as an anticancer agent.AIM To study the ...BACKGROUND Resistance to sorafenib has become a challenge in clinical treatment of hepatocellular carcinoma(HCC).Physcion is a common bioactive anthraquinone that has potential as an anticancer agent.AIM To study the effect of physcion on sensitizing HCC cells to sorafenib.METHODS Sorafenib-resistant HCC cells were established and treated with sorafenib and/or physcion.The cell viability,proliferation and apoptosis were measured by cell counting kit-8,colony formation,flow cytometry,and in vivo xenograft model.Glucose uptake,lactate acid production,extracellular acidification rate(ECAR),and oxygen consumption rate(OCR)were measured to analyze glycolysis.Expression of glycolysis-related regulators was assessed by western blotting.RESULTS The addition of physcion significantly enhanced the antitumor effects of sorafenib on sorafenib-resistant HCC cells,manifested by enhanced apoptosis and suppressed cell growth.The glucose uptake,lactate acid production,and ECAR were elevated,and OCR was suppressed by physcion treatment.The level of PIM1 was elevated and miR-370 was suppressed in sorafenib-resistant HCC cells compared with the parental cells,which was suppressed by physcion treatment.Inhibition of miR-370 notably reversed the effects of physcion on sorafenib-resistant HCC cells.CONCLUSION Our data indicated that physcion enhanced the sensitivity of HCC cells to sorafenib by enhancing miR-370 to suppress PIM1-promoted glycolysis.展开更多
肾脏疾病是临床上最常见的疾病之一,其进展至终末期肾衰竭将严重危害人类健康,因此对其发病机制的探讨研究以及寻求有效延缓疾病进展的治疗手段意义重大。莫洛尼小鼠白血病病毒前病毒插入位点1(provinal integration site 1 of murine l...肾脏疾病是临床上最常见的疾病之一,其进展至终末期肾衰竭将严重危害人类健康,因此对其发病机制的探讨研究以及寻求有效延缓疾病进展的治疗手段意义重大。莫洛尼小鼠白血病病毒前病毒插入位点1(provinal integration site 1 of murine leukemia virus,PIM1)是一种丝氨酸/苏氨酸激酶,具有调控细胞周期、凋亡、免疫、炎症和线粒体完整性等生物学功能。最近研究发现PIM1在肾脏疾病中也具有重要作用,本文围绕PIM1及其在肾脏疾病的发生发展中的作用机制做一综述,为临床肾脏疾病防治提供新的方向。展开更多
文摘利用InSAR监测矿山采空区地表沉降的过程中易在沉降中心周围产生失相干现象,导致形变结果缺失,无法获取准确的沉降区模型。基于此,提出了一种结合时序InSAR与PIM(Probability Integral Method)的监测方法,以云南省玉溪市大红山铁矿地下采区某工作面为研究对象,首先计算出时序InSAR累积沉降量,并提取其沉降区边缘的高相干点,结合少量沉降中心水准点,利用改进型PSO(Particle Swarm Optimization)算法获取PIM参数,并通过克里金插值法建立采空区地表整体沉降区模型,利用少量水准点数据对该方法的可靠性和精度进行验证,结果表明,该方法获得的沉降区分布与实际相吻合,其沉降区监测精度比单独使用时序InSAR或PIM的更高,可用于矿区地表沉降信息的准确获取。
基金Supported by National Natural Science Foundation of China,No.81960782.
文摘BACKGROUND Resistance to sorafenib has become a challenge in clinical treatment of hepatocellular carcinoma(HCC).Physcion is a common bioactive anthraquinone that has potential as an anticancer agent.AIM To study the effect of physcion on sensitizing HCC cells to sorafenib.METHODS Sorafenib-resistant HCC cells were established and treated with sorafenib and/or physcion.The cell viability,proliferation and apoptosis were measured by cell counting kit-8,colony formation,flow cytometry,and in vivo xenograft model.Glucose uptake,lactate acid production,extracellular acidification rate(ECAR),and oxygen consumption rate(OCR)were measured to analyze glycolysis.Expression of glycolysis-related regulators was assessed by western blotting.RESULTS The addition of physcion significantly enhanced the antitumor effects of sorafenib on sorafenib-resistant HCC cells,manifested by enhanced apoptosis and suppressed cell growth.The glucose uptake,lactate acid production,and ECAR were elevated,and OCR was suppressed by physcion treatment.The level of PIM1 was elevated and miR-370 was suppressed in sorafenib-resistant HCC cells compared with the parental cells,which was suppressed by physcion treatment.Inhibition of miR-370 notably reversed the effects of physcion on sorafenib-resistant HCC cells.CONCLUSION Our data indicated that physcion enhanced the sensitivity of HCC cells to sorafenib by enhancing miR-370 to suppress PIM1-promoted glycolysis.
文摘肾脏疾病是临床上最常见的疾病之一,其进展至终末期肾衰竭将严重危害人类健康,因此对其发病机制的探讨研究以及寻求有效延缓疾病进展的治疗手段意义重大。莫洛尼小鼠白血病病毒前病毒插入位点1(provinal integration site 1 of murine leukemia virus,PIM1)是一种丝氨酸/苏氨酸激酶,具有调控细胞周期、凋亡、免疫、炎症和线粒体完整性等生物学功能。最近研究发现PIM1在肾脏疾病中也具有重要作用,本文围绕PIM1及其在肾脏疾病的发生发展中的作用机制做一综述,为临床肾脏疾病防治提供新的方向。
