Prochlorococcus is the dominant population of the autotrophs in the oceanic area in theSouth China Sea; and contributes significantly to picoplanktonic (【2μm) carbon biomass. However, thereis no availabe data on its...Prochlorococcus is the dominant population of the autotrophs in the oceanic area in theSouth China Sea; and contributes significantly to picoplanktonic (【2μm) carbon biomass. However, thereis no availabe data on its growth the and productivity so far. In the present study, the in situ populationgrowth rate of Prochlorococus at the chlorophyll maximum layer at 6 .3°N, 110°E in the South China展开更多
Prochlorococcus, a tiny oxygenic photosynthetic picoplankton with unique pigment composition, has been found to be ubiquitous and abundant in the world oceans, and has been recognized to be closely related to living r...Prochlorococcus, a tiny oxygenic photosynthetic picoplankton with unique pigment composition, has been found to be ubiquitous and abundant in the world oceans, and has been recognized to be closely related to living resources and environmental issues. It has attracted the interest of marine biologists since its discovery, and field data on it over global oceans have accumulated rapidly in the past 10 years. In China, we have studied Prochlorococcus for 8 years, achieving a basic ecological understanding. The presence of Prochlorococcus in China seas, marginal seas of the west Pacific, was confirmed, and its distribution patterns were also brought to light. Prochlorococcus is very abundant in the South China Sea and the offshore regions of the East China Sea. It is seasonally present in the southeast part of the Yellow Sea and absent in the Bohai Sea. Temporal and spatial variations of the abundance of Prochlorococcus and their affecting factors, physiological and ecological characteristics of展开更多
Prochlorococcus,a group of marine cyanobacteria,is the most abundant and smallest oxygen-producing photosynthetic microorganism.It is mainly distributed in the euphotic zone of tropical and subtropical oceans and is a...Prochlorococcus,a group of marine cyanobacteria,is the most abundant and smallest oxygen-producing photosynthetic microorganism.It is mainly distributed in the euphotic zone of tropical and subtropical oceans and is a key participant in the marine biogeochemical cycle.Prochlorococcus has evolved continuously to adapt to the marine environment,and can be considered a recent evolutionary form of cyanobacteria,one of the most ancient life forms.The diverse ecotypes and subecotypes of Prochlorococcus increase its genomic diversity and environmental adaptability,allowing Prochlorococcus to become a dominant species in its environment.Here we review the ecotype classification and distribution,ecotype and subecotype differentiation,and environmental adaptability of Prochlorococcus.We also evaluate the role and significance of ecotype and sub-ecotype differentiation of Prochlorococcus,and discuss future research directions on this basis.展开更多
As the dominant primary producer in oligotrophic oceans, the unicellular picocyanobacterium Prochlorococcus is the smallest and most abundant photosynthetic phytoplankton in the world and plays an important role in ma...As the dominant primary producer in oligotrophic oceans, the unicellular picocyanobacterium Prochlorococcus is the smallest and most abundant photosynthetic phytoplankton in the world and plays an important role in marine carbon cycling.Cyanophages that infect Prochlorococcus influence the growth, carbon fixation, diversity, evolution, and environmental adaptation of their hosts. Here, we review studies on the isolation, genomics, and phylogenetic diversity of Prochlorococcus viruses and their interactions with Prochlorococcus. We also review the potential effects of Prochlorococcus viruses on biogeochemical cycling in the ocean.展开更多
The purpose of this study was to investigate the characteristics of transfer RNA(tRNA) responsible for the association between tRNA genes and genes of apparently foreign origin(genomic islands) in five high-light adap...The purpose of this study was to investigate the characteristics of transfer RNA(tRNA) responsible for the association between tRNA genes and genes of apparently foreign origin(genomic islands) in five high-light adapted Prochlorococcus strains.Both bidirectional best BLASTP(basic local alignment search tool for proteins) search and the conservation of gene order against each other were utilized to identify genomic islands,and 7 genomic islands were found to be immediately adjacent to tRNAs in Prochlorococcus marinus AS9601,11 in P.marinus MIT9515,8 in P.marinus MED4,6 in P.marinus MIT9301,and 6 in P.marinus MIT9312.Monte Carlo simulation showed that tRNA genes are hotspots for the integration of genomic islands in Prochlorococcus strains.The tRNA genes associated with genomic islands showed the following characteristics:(1) the association was biased towards a specific subset of all iso-accepting tRNA genes;(2) the codon usages of genes within genomic islands appear to be unrelated to the codons recognized by associated tRNAs;and,(3) the majority of the 3' ends of associated tRNAs lack CCA ends.These findings contradict previous hypotheses concerning the molecular basis for the frequent use of tRNA as the insertion site for foreign genetic materials.The analysis of a genomic island associated with a tRNA-Asn gene in P.marinus MIT9301 suggests that foreign genetic material is inserted into the host genomes by means of site-specific recombination,with the 3' end of the tRNA as the target,and during the process,a direct repeat of the 3' end sequence of a boundary tRNA(namely,a scar from the process of insertion) is formed elsewhere in the genomic island.Through the analysis of the sequences of these targets,it can be concluded that a region characterized by both high GC content and a palindromic structure is the preferred insertion site.展开更多
基金This study was supported by the 973 Project No.G2000078500,863 Project No.2001AA630509-2,NSFC Project No.4O232021,30170189 and 4017603
文摘Prochlorococcus is the dominant population of the autotrophs in the oceanic area in theSouth China Sea; and contributes significantly to picoplanktonic (【2μm) carbon biomass. However, thereis no availabe data on its growth the and productivity so far. In the present study, the in situ populationgrowth rate of Prochlorococus at the chlorophyll maximum layer at 6 .3°N, 110°E in the South China
基金This work was supported by the National Natural Science Foundation of China (Grant Nos. 39625008 and 49876033) the National Basic Research Programme (Grant No. G2000078500).
文摘Prochlorococcus, a tiny oxygenic photosynthetic picoplankton with unique pigment composition, has been found to be ubiquitous and abundant in the world oceans, and has been recognized to be closely related to living resources and environmental issues. It has attracted the interest of marine biologists since its discovery, and field data on it over global oceans have accumulated rapidly in the past 10 years. In China, we have studied Prochlorococcus for 8 years, achieving a basic ecological understanding. The presence of Prochlorococcus in China seas, marginal seas of the west Pacific, was confirmed, and its distribution patterns were also brought to light. Prochlorococcus is very abundant in the South China Sea and the offshore regions of the East China Sea. It is seasonally present in the southeast part of the Yellow Sea and absent in the Bohai Sea. Temporal and spatial variations of the abundance of Prochlorococcus and their affecting factors, physiological and ecological characteristics of
基金supported by the National Natural Science Foundation of China(Grant Nos.41906110,41876150,41861144018&91751207)the Fundamental Research Funds for the Central Universities(Grant Nos.26420190053,20720190141&20720170107)the State Key Laboratory of Marine Environmental Science(Xiamen University)Visiting Fellowship Program(Grant No.MELRS1911)。
文摘Prochlorococcus,a group of marine cyanobacteria,is the most abundant and smallest oxygen-producing photosynthetic microorganism.It is mainly distributed in the euphotic zone of tropical and subtropical oceans and is a key participant in the marine biogeochemical cycle.Prochlorococcus has evolved continuously to adapt to the marine environment,and can be considered a recent evolutionary form of cyanobacteria,one of the most ancient life forms.The diverse ecotypes and subecotypes of Prochlorococcus increase its genomic diversity and environmental adaptability,allowing Prochlorococcus to become a dominant species in its environment.Here we review the ecotype classification and distribution,ecotype and subecotype differentiation,and environmental adaptability of Prochlorococcus.We also evaluate the role and significance of ecotype and sub-ecotype differentiation of Prochlorococcus,and discuss future research directions on this basis.
基金supported by the Qingdao National Laboratory for Marine Science and Technology (Grant No. QNLM2016ORP0303)the National Natural Science Foundation of China (Grant Nos. 41522603 & 91428308)the China National Offshore Oil Corporation (Grant Nos. CNOOC-KJ125FZDXM00TJ001-2014 & CNOOC-KJ125FZDXM00ZJ001-2014)
文摘As the dominant primary producer in oligotrophic oceans, the unicellular picocyanobacterium Prochlorococcus is the smallest and most abundant photosynthetic phytoplankton in the world and plays an important role in marine carbon cycling.Cyanophages that infect Prochlorococcus influence the growth, carbon fixation, diversity, evolution, and environmental adaptation of their hosts. Here, we review studies on the isolation, genomics, and phylogenetic diversity of Prochlorococcus viruses and their interactions with Prochlorococcus. We also review the potential effects of Prochlorococcus viruses on biogeochemical cycling in the ocean.
基金(No. 2006AA10A102) supported by the National High-Tech R & D Program (863) of China
文摘The purpose of this study was to investigate the characteristics of transfer RNA(tRNA) responsible for the association between tRNA genes and genes of apparently foreign origin(genomic islands) in five high-light adapted Prochlorococcus strains.Both bidirectional best BLASTP(basic local alignment search tool for proteins) search and the conservation of gene order against each other were utilized to identify genomic islands,and 7 genomic islands were found to be immediately adjacent to tRNAs in Prochlorococcus marinus AS9601,11 in P.marinus MIT9515,8 in P.marinus MED4,6 in P.marinus MIT9301,and 6 in P.marinus MIT9312.Monte Carlo simulation showed that tRNA genes are hotspots for the integration of genomic islands in Prochlorococcus strains.The tRNA genes associated with genomic islands showed the following characteristics:(1) the association was biased towards a specific subset of all iso-accepting tRNA genes;(2) the codon usages of genes within genomic islands appear to be unrelated to the codons recognized by associated tRNAs;and,(3) the majority of the 3' ends of associated tRNAs lack CCA ends.These findings contradict previous hypotheses concerning the molecular basis for the frequent use of tRNA as the insertion site for foreign genetic materials.The analysis of a genomic island associated with a tRNA-Asn gene in P.marinus MIT9301 suggests that foreign genetic material is inserted into the host genomes by means of site-specific recombination,with the 3' end of the tRNA as the target,and during the process,a direct repeat of the 3' end sequence of a boundary tRNA(namely,a scar from the process of insertion) is formed elsewhere in the genomic island.Through the analysis of the sequences of these targets,it can be concluded that a region characterized by both high GC content and a palindromic structure is the preferred insertion site.