Laboratory experiments were conducted to determine the effects of total dissolved gas(TDG) supersaturation on acute lethality and avoidance responses in juvenile rock carp(Procypris rabaudi Tchang).The juvenile rock c...Laboratory experiments were conducted to determine the effects of total dissolved gas(TDG) supersaturation on acute lethality and avoidance responses in juvenile rock carp(Procypris rabaudi Tchang).The juvenile rock carp were exposed to water with different levels of supersaturation(105%,115%,120%,125%,130%,135%,140%,and 145%) and depth of 0.20 m at 25 °C for 60 h.Median lethal time(LT50) was used to assess the lethal responses corresponding to different levels of gas supersaturation.The results show that half of the juvenile rock carp died at the 120%,125%,130%,135%,140%,and 145% levels of supersaturation,and the LT50 corresponding to different levels of supersaturation was 18.7,15.4,8.2,6.6,3.5,and 1.7 h.When the level of supersaturated water is below 115%,the mortality is negligible.Avoidance responses were observed 5 min after the fish were put into equilibrated water(99%,0.08 m deep) and water with different supersaturated levels(105%,115%,125%,135%,and 145%,0.08 m deep) at 25 °C.The fish exhibited strong avoidance responses in supersaturated water when the gas supersaturation was above 135%.However,they exhibited an obvious preference to supersaturated water when the gas supersaturation was below 115%.Thus,the juvenile rock carp can likely survive in water with a supersaturated level of 115%.展开更多
Total dissolved gas supersaturation(TDGS) appears when the pressures of gases in a solution exceed the barometric pressures.TDGS is often caused by flood discharge at dams.It may lead to gas bubble disease(GBD) for fi...Total dissolved gas supersaturation(TDGS) appears when the pressures of gases in a solution exceed the barometric pressures.TDGS is often caused by flood discharge at dams.It may lead to gas bubble disease(GBD) for fish and biochemical responses of selected fish and other aquatic organisms.The purpose of this study was to determine the impact of long-term TDGS levels on the growth and biochemical responses of rock carp(Procypris rabaudi Tchang) dwelling in the upper reaches of the Yangtze River.Three-year-old rock carp were exposed to TDGS levels at 100%,104%,108%,112%,and 116% for 42 d.Samples were taken every 7 d after the start of the trial in order to determine catalase(CAT) and superoxide dismutase(SOD) activities in gill and muscle tissues.Samples were taken at Days 0 and 42 of exposure to determine growth rate.Little effect was found on growth rate in all treatment groups.SOD and CAT activities varied in different tissues,according to time of exposure and TDGS levels.The biochemical response of fish exposed to TDGS was more obvious in gill tissue than in muscle tissue.Surveys of SOD and CAT activities in different tissues offer important information about the effect of TDGS on the rare fish in the Yangtze River,and may help evaluate the risk to the aquatic eco-environment and aquatic ecosystem in the downstream of the Yangtze River.展开更多
基金Project (No. 50979063) supported by the National Natural Science Foundation of China
文摘Laboratory experiments were conducted to determine the effects of total dissolved gas(TDG) supersaturation on acute lethality and avoidance responses in juvenile rock carp(Procypris rabaudi Tchang).The juvenile rock carp were exposed to water with different levels of supersaturation(105%,115%,120%,125%,130%,135%,140%,and 145%) and depth of 0.20 m at 25 °C for 60 h.Median lethal time(LT50) was used to assess the lethal responses corresponding to different levels of gas supersaturation.The results show that half of the juvenile rock carp died at the 120%,125%,130%,135%,140%,and 145% levels of supersaturation,and the LT50 corresponding to different levels of supersaturation was 18.7,15.4,8.2,6.6,3.5,and 1.7 h.When the level of supersaturated water is below 115%,the mortality is negligible.Avoidance responses were observed 5 min after the fish were put into equilibrated water(99%,0.08 m deep) and water with different supersaturated levels(105%,115%,125%,135%,and 145%,0.08 m deep) at 25 °C.The fish exhibited strong avoidance responses in supersaturated water when the gas supersaturation was above 135%.However,they exhibited an obvious preference to supersaturated water when the gas supersaturation was below 115%.Thus,the juvenile rock carp can likely survive in water with a supersaturated level of 115%.
基金Project (No. 50979063) supported by the National Natural Science Foundation of China
文摘Total dissolved gas supersaturation(TDGS) appears when the pressures of gases in a solution exceed the barometric pressures.TDGS is often caused by flood discharge at dams.It may lead to gas bubble disease(GBD) for fish and biochemical responses of selected fish and other aquatic organisms.The purpose of this study was to determine the impact of long-term TDGS levels on the growth and biochemical responses of rock carp(Procypris rabaudi Tchang) dwelling in the upper reaches of the Yangtze River.Three-year-old rock carp were exposed to TDGS levels at 100%,104%,108%,112%,and 116% for 42 d.Samples were taken every 7 d after the start of the trial in order to determine catalase(CAT) and superoxide dismutase(SOD) activities in gill and muscle tissues.Samples were taken at Days 0 and 42 of exposure to determine growth rate.Little effect was found on growth rate in all treatment groups.SOD and CAT activities varied in different tissues,according to time of exposure and TDGS levels.The biochemical response of fish exposed to TDGS was more obvious in gill tissue than in muscle tissue.Surveys of SOD and CAT activities in different tissues offer important information about the effect of TDGS on the rare fish in the Yangtze River,and may help evaluate the risk to the aquatic eco-environment and aquatic ecosystem in the downstream of the Yangtze River.
