Objective： The aim of the study was to explore the bone morphogenetic protein 4 （BMP-4） how to regulate pro- lactin （PRL） secreting, cell proliferation and invasiveness in human prolactinorna. Methods： Ten patie...Objective： The aim of the study was to explore the bone morphogenetic protein 4 （BMP-4） how to regulate pro- lactin （PRL） secreting, cell proliferation and invasiveness in human prolactinorna. Methods： Ten patients who diagnosed as prolactinoma by clinical characteristics and pathology were divided into two groups, one was sensitive to Brornocriptine, the other was insensitive to Brornocriptine. Every case was conducted by primal cell culture then treated by different concentrations of BMP-4. The cell configuration was observed and PRL hormone was measured in different times. The expressions of BMP-4 rnRNA and BMP-4 in 37 cases of prolactinorna and 8 cases of normal pituitary tissues samples were detected by immunohistochemical and in situ hybridization technique, and the results were analyzed by statistic methods. Results： BMP-4 （5 ng/rnL） could accelerate the secreting of PRL in prolactinorna cell, and it could reach the greatest effect in the concentration of 20 ng/mL. BMP-4 could increase prolactinoma cell proliferation, but when the concentration was 50 ng/rnL, the BMP-4 effect of increasing secreting decreased. When 100 ng/mL, the cell began to die. The effects of the BMP-4 in sensitive group and insensitive group had no difference. The BMP-4 was highly expressed in the prolactinornas and was positively related with the invasiveness grades. Conclusion： BMP-4 have positive regulation in prolactinoma secreting, proliferation, invasiveness effects, BMP-4 probably has the important role in prolactinoma pathogenesis.展开更多
Objective To study the effect of Chinese herbs preparation Bu-shen on pituitary prolactin-releasing peptide receptor (PrRP-R) and prolactin receptor (PRL-R) expression in the bromocriptine-induced rat abortion model M...Objective To study the effect of Chinese herbs preparation Bu-shen on pituitary prolactin-releasing peptide receptor (PrRP-R) and prolactin receptor (PRL-R) expression in the bromocriptine-induced rat abortion model Materials & Methods Female SD rats were divided into five groups randomly. Group A,B,C and D were injected with bromocriptine [0. 3 mg/(kg · d)] during the pregnant day 6～ 8, respectively. Group B was given powder of Bu-shen herbs [3 g/(kg · d)]. Group C was injected with prolactin (8 IU) twice a day and Group D with progesterone [(8 mg/kg · d) in the pregnant day 1～ 11. The rats in Group E were normal pregnant rats. All these rats were killed at the pregnant day 12 to observe the expression of PrRP-R, PRL-R from the pituitary using RT-PCR. Results The pregnant rate and total number of litters in Group A were significantly lower than those in Group B, C, D and E (P＜0. 01) and the expression of PrRP-R and PRL-R in the pituitary of Group A was higher than that of any other group. Conclusion Preparation Bu-shen plays a regulatory role in the secretion of prolactin possibly via modulating rats' hypothalamus and pituitary.展开更多
Broody behavior is regulated by hypothalamic prolactin secretion,which seriously affects egg production in poulty production.Numerous studies have provided evidence that animal behavior is governed by dynamic bidirect...Broody behavior is regulated by hypothalamic prolactin secretion,which seriously affects egg production in poulty production.Numerous studies have provided evidence that animal behavior is governed by dynamic bidirectional communication between specific gut bacteria and their host via the brain-gut-microbiome axis.However,little research focused on how the gut microbiota influence broody behavior in poultry.In this study,Zhedong white geese in laying and brooding phases were selected.Ten differentially abundant bacteria in cecum were detected between brooding and laying geese through metagenomic analyses and 16S rRNA sequencing(P<0.05),and Bacteroides fragilis was specifically identified as a key driver species in the brooding geese.Moverover,the serum metabolites were quantified,and the 313 differentially abundant metabolites were found between the two groups of different physiological geese.They were primarily enriched in the tryptophan metabolism pathways.Pearson correlation analyses revealed there was a significant positive correlation between B.fragilis abundance and the context of 11 tryptophan metabolism-related metabolites(such as serotonin,etc.)in broody geese,which hinted that those tryptophan metabolites might be produced or driven by B.fragilis.Finally,the serum hormone levels were also measured.We found there was a positive correlation between B.fragilis abundance and content of serotonin.Besides,prolactin secreted by the pituitary gland was greater in brooding geese than that in laying geese,which was also highly correlated with B.fragilis abundance.This result implied that B.fragilis could promote the secretion of prolactin by the pituitary gland.Together,the current study findings provided the information on gut microbiota influencing broody behavior,B.fragilis produced or driven more serum serotonin,and stimulated the pituitary gland to secret more prolactin,which potentially offered a new enlightenment for the intervention of broody behavior in poultry.展开更多
目的探究精神分裂症患者脑部代谢激素同型半胱氨酸(homocysteine,HCY)、催乳素(prolactin,PRL)与认知功能的相关性,挖掘重复经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)联合奥氮平治疗该病的效果,以期解读脑部代谢...目的探究精神分裂症患者脑部代谢激素同型半胱氨酸(homocysteine,HCY)、催乳素(prolactin,PRL)与认知功能的相关性,挖掘重复经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)联合奥氮平治疗该病的效果,以期解读脑部代谢激素与认知功能的影响机制,同时,挖掘rTMS联合奥氮平这种内外结合治疗方式来丰富该领域研究,为相关研究提供借鉴。方法选取2021年6月至2023年8月笔者医院治疗的128例精神分裂症患者资料进行回顾性分析,根据治疗方式的不同划分为观察组72例(rTMS联合奥氮平治疗)和对照组56例(奥氮平单药治疗)。分析两组患者血清HCY、PRL、阳性和阴性综合征量表(positive and negative syndrome scale,PANSS)、白细胞介素(interleukin,IL)-12,肿瘤坏死因子(tumor necrosis factor,TNF)-α及其相关性。比较两组患者不良反应发生率情况。结果治疗后,观察组PRL、HCY、IL-12、TNF-α水平低于对照组,差异有统计学意义(P<0.05)。交互作用、时点作用、组间作用下,两组患者的PANSS评分会因为不同治疗方式的时间推移而发生变化,但观察组均低于对照组,差异有统计学意义(P<0.05)。治疗方法与HCY、PRL、IL-12、TNF-α、PANSS均呈负相关,且HCY、PRL、IL–12、TNF–α、PANSS两两间为正相关(P<0.05)。观察组头痛、记忆受损、嗜睡、不良反应事件等发生率低于对照组,差异有统计学意义(P<0.05)。结论经rTMS联合奥氮平治疗后,可以降低精神分裂症患者HCY、PRL激素水平,减轻炎症,减少不良反应,改善患者认知功能。展开更多
文摘Objective： The aim of the study was to explore the bone morphogenetic protein 4 （BMP-4） how to regulate pro- lactin （PRL） secreting, cell proliferation and invasiveness in human prolactinorna. Methods： Ten patients who diagnosed as prolactinoma by clinical characteristics and pathology were divided into two groups, one was sensitive to Brornocriptine, the other was insensitive to Brornocriptine. Every case was conducted by primal cell culture then treated by different concentrations of BMP-4. The cell configuration was observed and PRL hormone was measured in different times. The expressions of BMP-4 rnRNA and BMP-4 in 37 cases of prolactinorna and 8 cases of normal pituitary tissues samples were detected by immunohistochemical and in situ hybridization technique, and the results were analyzed by statistic methods. Results： BMP-4 （5 ng/rnL） could accelerate the secreting of PRL in prolactinorna cell, and it could reach the greatest effect in the concentration of 20 ng/mL. BMP-4 could increase prolactinoma cell proliferation, but when the concentration was 50 ng/rnL, the BMP-4 effect of increasing secreting decreased. When 100 ng/mL, the cell began to die. The effects of the BMP-4 in sensitive group and insensitive group had no difference. The BMP-4 was highly expressed in the prolactinornas and was positively related with the invasiveness grades. Conclusion： BMP-4 have positive regulation in prolactinoma secreting, proliferation, invasiveness effects, BMP-4 probably has the important role in prolactinoma pathogenesis.
文摘Objective To study the effect of Chinese herbs preparation Bu-shen on pituitary prolactin-releasing peptide receptor (PrRP-R) and prolactin receptor (PRL-R) expression in the bromocriptine-induced rat abortion model Materials & Methods Female SD rats were divided into five groups randomly. Group A,B,C and D were injected with bromocriptine [0. 3 mg/(kg · d)] during the pregnant day 6～ 8, respectively. Group B was given powder of Bu-shen herbs [3 g/(kg · d)]. Group C was injected with prolactin (8 IU) twice a day and Group D with progesterone [(8 mg/kg · d) in the pregnant day 1～ 11. The rats in Group E were normal pregnant rats. All these rats were killed at the pregnant day 12 to observe the expression of PrRP-R, PRL-R from the pituitary using RT-PCR. Results The pregnant rate and total number of litters in Group A were significantly lower than those in Group B, C, D and E (P＜0. 01) and the expression of PrRP-R and PRL-R in the pituitary of Group A was higher than that of any other group. Conclusion Preparation Bu-shen plays a regulatory role in the secretion of prolactin possibly via modulating rats' hypothalamus and pituitary.
基金supported by the Modern Agro-industry Technology Research System,China(CARS-42-3)the“JBGS”Project of Seed Industry Revitalization in Jiangsu Province,China(JBGS(2021)023)the Project in Ministry of Agriculture and Rural Affairs of China(19211168).
文摘Broody behavior is regulated by hypothalamic prolactin secretion,which seriously affects egg production in poulty production.Numerous studies have provided evidence that animal behavior is governed by dynamic bidirectional communication between specific gut bacteria and their host via the brain-gut-microbiome axis.However,little research focused on how the gut microbiota influence broody behavior in poultry.In this study,Zhedong white geese in laying and brooding phases were selected.Ten differentially abundant bacteria in cecum were detected between brooding and laying geese through metagenomic analyses and 16S rRNA sequencing(P<0.05),and Bacteroides fragilis was specifically identified as a key driver species in the brooding geese.Moverover,the serum metabolites were quantified,and the 313 differentially abundant metabolites were found between the two groups of different physiological geese.They were primarily enriched in the tryptophan metabolism pathways.Pearson correlation analyses revealed there was a significant positive correlation between B.fragilis abundance and the context of 11 tryptophan metabolism-related metabolites(such as serotonin,etc.)in broody geese,which hinted that those tryptophan metabolites might be produced or driven by B.fragilis.Finally,the serum hormone levels were also measured.We found there was a positive correlation between B.fragilis abundance and content of serotonin.Besides,prolactin secreted by the pituitary gland was greater in brooding geese than that in laying geese,which was also highly correlated with B.fragilis abundance.This result implied that B.fragilis could promote the secretion of prolactin by the pituitary gland.Together,the current study findings provided the information on gut microbiota influencing broody behavior,B.fragilis produced or driven more serum serotonin,and stimulated the pituitary gland to secret more prolactin,which potentially offered a new enlightenment for the intervention of broody behavior in poultry.
文摘目的探究精神分裂症患者脑部代谢激素同型半胱氨酸(homocysteine,HCY)、催乳素(prolactin,PRL)与认知功能的相关性,挖掘重复经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)联合奥氮平治疗该病的效果,以期解读脑部代谢激素与认知功能的影响机制,同时,挖掘rTMS联合奥氮平这种内外结合治疗方式来丰富该领域研究,为相关研究提供借鉴。方法选取2021年6月至2023年8月笔者医院治疗的128例精神分裂症患者资料进行回顾性分析,根据治疗方式的不同划分为观察组72例(rTMS联合奥氮平治疗)和对照组56例(奥氮平单药治疗)。分析两组患者血清HCY、PRL、阳性和阴性综合征量表(positive and negative syndrome scale,PANSS)、白细胞介素(interleukin,IL)-12,肿瘤坏死因子(tumor necrosis factor,TNF)-α及其相关性。比较两组患者不良反应发生率情况。结果治疗后,观察组PRL、HCY、IL-12、TNF-α水平低于对照组,差异有统计学意义(P<0.05)。交互作用、时点作用、组间作用下,两组患者的PANSS评分会因为不同治疗方式的时间推移而发生变化,但观察组均低于对照组,差异有统计学意义(P<0.05)。治疗方法与HCY、PRL、IL-12、TNF-α、PANSS均呈负相关,且HCY、PRL、IL–12、TNF–α、PANSS两两间为正相关(P<0.05)。观察组头痛、记忆受损、嗜睡、不良反应事件等发生率低于对照组,差异有统计学意义(P<0.05)。结论经rTMS联合奥氮平治疗后,可以降低精神分裂症患者HCY、PRL激素水平,减轻炎症,减少不良反应,改善患者认知功能。
