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A Multimodal Critical Discourse Analysis of Lingnan Cultural Promotional Videos on Social Media
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作者 Lu Jiang 《Journal of Contemporary Educational Research》 2024年第4期59-68,共10页
In recent years,more and more directors of culture and tourism have taken part in the promotion of local cultural tourism by cross-dressing,talent shows,and pushing their limits on self-media platforms.This study inve... In recent years,more and more directors of culture and tourism have taken part in the promotion of local cultural tourism by cross-dressing,talent shows,and pushing their limits on self-media platforms.This study investigates short videos of Lingnan culture promoted by directors general and deputy directors general of the Culture,Radio,Television,Tourism,and Sports Bureau of counties and cities in Guangdong Province on social media by the method of multimodal critical discourse analysis.The analysis of 33 videos shows that Lingnan culture is a domineering and confident culture,historical culture,graceful and elegant culture,and vibrant and active culture.Domineering and confident culture is embedded in the utterances and behaviors of the directors general or deputy directors general in the video.Historical culture is realized through the conversation with historical figures through time travel.Graceful and elegant culture is constructed in the depiction of sceneries and the depiction of characters’manners.Vibrant and active culture is represented in the depiction of the characters’actional process and analytical process. 展开更多
关键词 Lingnan culture Multimodal critical discourse analysis promotional videos TikTok WeChat
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The Discourse-Historical Approach of Identity Construction in Corporate Promotional Videos for External Publicity Image
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作者 Xiaodong Wang Jing Yu 《Proceedings of Business and Economic Studies》 2024年第2期178-183,共6页
Corporate identity construction of external publicity image is an important part of the development of enterprises.Based on Wodak’s discourse-historical approach,this study takes the text of COFCO’s English promotio... Corporate identity construction of external publicity image is an important part of the development of enterprises.Based on Wodak’s discourse-historical approach,this study takes the text of COFCO’s English promotional video as the research object,analyzes the corporate brand image,media image,organizational image,and environmental image constructed by the enterprises from three steps:linguistic expression,discourse strategy,and theme to provide references for Chinese enterprises to enhance their international influence. 展开更多
关键词 Corporate promotional videos External publicity image Discourse-historical approach
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Theoretical study on the morphology of cobalt nanoparticles modulated by alkali metal promoters
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作者 Xiaobin Geng Hui Yang +3 位作者 Wenping Guo Xiaotong Liu Tao Yang Jinjia Liu 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2023年第10期2006-2013,共8页
Cobalt nanoparticles(NPs)catalysts are extensively used in heterogeneous catalytic reactions,and the addition of alkali metal promoters is a common method to modulate the catalytic performance because the catalyst'... Cobalt nanoparticles(NPs)catalysts are extensively used in heterogeneous catalytic reactions,and the addition of alkali metal promoters is a common method to modulate the catalytic performance because the catalyst's surface structures and morphologies are sensitive to the addition of promoters.However,the underlying modulation trend remains unclear.Herein,the adsorption of alkali metal promoters(Na and K)on the surfaces of face-centered-cubic(FCC)and hexagonal-closest packed(HCP)polymorphous cobalt was systematically investigated using density functional theory.Furthermore,the effect of alkali promoters on surface energies and nanoparticle morphologies was revealed on the basis of Wulff theory.For FCC-Co,the exposed area of the(111)facet in the nanoparticle increases with the adsorption coverage of alkali metal oxide.Meanwhile,the(311),(110),and(100)facets would disappear under the higher adsorption coverage of alkali metals.For HCPCo,the Wulff morphology is dominated by the(0001)and(1011)facets and is independent of the alkali metal adsorption coverage.This work provides insights into morphology modulation by alkali metal promoters for the rational design and synthesis of cobalt-based nanomaterials with desired facets and morphologies. 展开更多
关键词 cobalt nanoparticles alkali promoter density functional theory crystal morphology Wulff construction
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Dual-targeting AAV9P1-mediated neuronal reprogramming in a mouse model of traumatic brain injury 被引量:1
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作者 Jingzhou Liu Xin Xin +8 位作者 Jiejie Sun Yueyue Fan Xun Zhou Wei Gong Meiyan Yang Zhiping Li Yuli Wang Yang Yang Chunsheng Gao 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第3期629-635,共7页
Traumatic brain injury results in neuronal loss and glial scar formation.Replenishing neurons and eliminating the consequences of glial scar formation are essential for treating traumatic brain injury.Neuronal reprogr... Traumatic brain injury results in neuronal loss and glial scar formation.Replenishing neurons and eliminating the consequences of glial scar formation are essential for treating traumatic brain injury.Neuronal reprogramming is a promising strategy to convert glial scars to neural tissue.However,previous studies have reported inconsistent results.In this study,an AAV9P1 vector incorporating an astrocyte-targeting P1 peptide and glial fibrillary acidic protein promoter was used to achieve dual-targeting of astrocytes and the glial scar while minimizing off-target effects.The results demonstrate that AAV9P1 provides high selectivity of astrocytes and reactive astrocytes.Moreover,neuronal reprogramming was induced by downregulating the polypyrimidine tract-binding protein 1 gene via systemic administration of AAV9P1 in a mouse model of traumatic brain injury.In summary,this approach provides an improved gene delivery vehicle to study neuronal programming and evidence of its applications for traumatic brain injury. 展开更多
关键词 AAV9P1 ASTROCYTES astrocyte-to-neuron conversion GFAP promoter glial scar induced neurons neuronal reprogramming P1 peptide PTBP1 traumatic brain injury
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DNA G-Quadruplexes as Targets for Natural Product Drug Discovery
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作者 Kai-Bo Wang Yingying Wang +1 位作者 Jonathan Dickerhoff Danzhou Yang 《Engineering》 SCIE EI CAS CSCD 2024年第7期39-51,共13页
DNA guanine(G)-quadruplexes(G4s)are unique secondary structures formed by two or more stacked Gtetrads in G-rich DNA sequences.These structures have been found to play a crucial role in highly transcribed genes,especi... DNA guanine(G)-quadruplexes(G4s)are unique secondary structures formed by two or more stacked Gtetrads in G-rich DNA sequences.These structures have been found to play a crucial role in highly transcribed genes,especially in cancer-related oncogenes,making them attractive targets for cancer therapeutics.Significantly,targeting oncogene promoter G4 structures has emerged as a promising strategy to address the challenge of undruggable and drug-resistant proteins,such as MYC,BCL2,KRAS,and EGFR.Natural products have long been an important source of drug discovery,particularly in the fields of cancer and infectious diseases.Noteworthy progress has recently been made in the discovery of naturally occurring DNA G4-targeting drugs.Numerous DNA G4s,such as MYC-G4,BCL2-G4,KRAS-G4,PDGFR-b-G4,VEGF-G4,and telomeric-G4,have been identified as potential targets of natural products,including berberine,telomestatin,quindoline,sanguinarine,isaindigotone,and many others.Herein,we summarize and evaluate recent advancements in natural and nature-derived DNA G4 binders,focusing on understanding the structural recognition of DNA G4s by small molecules derived from nature.We also discuss the challenges and opportunities associated with developing drugs that target DNA G4s. 展开更多
关键词 G-QUADRUPLEX Natural products ALKALOIDS CANCER PROMOTER
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Measurement,associations,patterns,and promotion:Unveiling vital areas in physical activity epidemiology
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作者 Kara M.Whitaker Guest editor Michael J.LaMonte 《Journal of Sport and Health Science》 SCIE CAS CSCD 2024年第5期605-607,共3页
Physical activity(PA)epidemiology first emerged as a new field of study around the 1950s with publication of seminal investigations on London civil servants1 and has continued to evolve as a scientific discipline to p... Physical activity(PA)epidemiology first emerged as a new field of study around the 1950s with publication of seminal investigations on London civil servants1 and has continued to evolve as a scientific discipline to present time.This subdiscipline of epidemiology studies the frequencies,distributions,and dynamics of PA and its impact on human health,morbidity,and mortality.Research over the past 75 years has demonstrated numerous health benefits of PA.Based on these research findings,the World Health Organization recommends adults aged 18-64 years engage in at least 150-300 min of moderate-intensity aerobic PA per week,or at least 75-150 min of vigorous-intensity aerobic PA per week,or a combination of both intensities. 展开更多
关键词 EPIDEMIOLOGY PROMOTION continued
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Pig H3K4me3,H3K27ac,and gene expression profiles reveal reproductive tissue-specific activity of transposable elements
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作者 Tao Jiang Zhi-Min Zhou +6 位作者 Zi-Qi Ling Qing Zhang Zhong-Zi Wu Jia-Wen Yang Si-Yu Yang Bin Yang Lu-Sheng Huang 《Zoological Research》 SCIE CSCD 2024年第1期138-151,共14页
Regulatory sequences and transposable elements(TEs)account for a large proportion of the genomic sequences of species;however,their roles in gene transcription,especially tissue-specific expression,remain largely unkn... Regulatory sequences and transposable elements(TEs)account for a large proportion of the genomic sequences of species;however,their roles in gene transcription,especially tissue-specific expression,remain largely unknown.Pigs serve as an excellent animal model for studying genomic sequence biology due to the extensive diversity among their wild and domesticated populations.Here,we conducted an integrated analysis using H3K27ac ChIP-seq,H3K4me3 ChIP-seq,and RNA-seq data from 10 different tissues of seven fetuses and eight closely related adult pigs.We aimed to annotate the regulatory elements and TEs to elucidate their associations with histone modifications and mRNA expression across different tissues and developmental stages.Based on correlation analysis between mRNA expression and H3K27ac and H3K4me3 peak activity,results indicated that H3K27ac exhibited stronger associations with gene expression than H3K4me3.Furthermore,1.45%of TEs overlapped with either the H3K27ac or H3K4me3 peaks,with the majority displaying tissue-specific activity.Notably,a TE subfamily(LTR4C_SS),containing binding motifs for SIX1 and SIX4,showed specific enrichment in the H3K27ac peaks of the adult and fetal ovaries.RNA-seq analysis also revealed widespread expression of TEs in the exons or promoters of genes,including 4688 TE-containing transcripts with distinct development stage-specific and tissue-specific expression.Of note,1967 TE-containing transcripts were enriched in the testes.We identified a long terminal repeat(LTR),MLT1F1,acting as a testis-specific alternative promoter in SRPK2(a cell cycle-related protein kinase)in our pig dataset.This element was also conserved in humans and mice,suggesting either an ancient integration of TEs in genes specifically expressed in the testes or parallel evolutionary patterns.Collectively,our findings demonstrate that TEs are deeply embedded in the genome and exhibit important tissue-specific biological functions,particularly in the reproductive organs. 展开更多
关键词 Transposable elements PORCINE Histone modification Alternative promoter TE-containing transcript
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Is it possible to run after positive affective experiences instead of health benefits without tackling social inequalities?
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作者 Raphael H.O.Araujo Andre O.Werneck +1 位作者 Gilmar M.de Jesus Danilo R.P.Silva 《Journal of Sport and Health Science》 SCIE CAS CSCD 2024年第6期756-758,共3页
We recently read the opinion piece titled“Why people should run after positive affective experiences instead of health benefits”,which was carried out by Maltagliati et al.^(1)and published in the Journal of Sport a... We recently read the opinion piece titled“Why people should run after positive affective experiences instead of health benefits”,which was carried out by Maltagliati et al.^(1)and published in the Journal of Sport and Health Science.The article started an important discussion about a physical activity(PA)promotion approach that encourages the use of affective experience to PA activity.Although we agree with the authors’point of view and believe that its message can generate an important insight for PA promotion,the authors presented a scenario where behavior change seems to be exclusively dependent on the individual.Recognizing the existence of other barriers to an active lifestyle(e.g.,social inequalities)could enhance the authors’suggested approach because many people live in conditions that reduce their opportunities to engage in affective experiences related to PA;furthermore,affective experiences can vary depending on individual context. 展开更多
关键词 PROMOTION INEQUALITIES furthermore
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Understanding the catalytic performance and deactivation behaviour of second-promoter doped Pt/WO_(χ)/γ-Al_(2)O_(3) catalysts in the glycerol hydrogenolysis for selective and cleaner production of 1,3-propanediol
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作者 Rafik Rajjak Shaikh Sittichai Damruang +2 位作者 Rais Ahmad Khan Supareak Praserthdam Piyasan Praserthdam 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第7期486-507,共22页
The selective aqueous-phase glycerol hydrogenolysis is a promising reaction to produce commercially useful 1,3-propanediol(1,3-PDO).The Pt-WOx bifunctional catalyst can catalyse the glycerol hydrogenol-ysis but the ca... The selective aqueous-phase glycerol hydrogenolysis is a promising reaction to produce commercially useful 1,3-propanediol(1,3-PDO).The Pt-WOx bifunctional catalyst can catalyse the glycerol hydrogenol-ysis but the catalyst deactivation via sintering,metal leaching,and coking can predominantly occur in the aqueous phase reaction.In this work,the effect of reaction temperature,pressure and second promoter(Cu,Fe,Rh,Mn,Re,Ru,Ir,Sn,B,and P)on catalytic performance and deactivation behaviour of Pt/WOx/-Al2O3 was investigated.When doped with Rh,Mn,Re,Ru,Ir,B,and P,the second promoter boosts catalytic activity by promoting great dispersion of Pt on support and increasing Pt surface area.The increased Bronsted acid sites lead to selective synthesis of 1,3-PDO than 1,2-propanediol(1,2-PDO).The characterization studies of fresh and spent catalysts reveal that the main cause of catalyst deactivation is the Pt sintering,as interpreted based on XRD,CO chemisorption,and TEM analyses.The Pt sintering is affected depending on the second promoter that can either or reduce the interaction between Pt,WO_(χ)/γ and Al_(2)O_(3).As an electron acceptor of Pt in Pt/WO_(χ)/γ-Al_(2)O_(3),Re and Mn as second promoters resulted in increased Pt^(2+) on the catalytic surface,which strengthens the contact between Pt andγ-Al_(2)O_(3) and WO_(χ),resulting in a decrease in Pt sintering.The metal leaching and coking are not affected by the presence of second promoter.The catalyst modified with a second promoter possesses improved catalytic activity and 1,3-PDO production,however the stability continues to remain a challenge.The present work unrav-elled the determining parameters of catalytic activity and deactivation,thus providing a promising pro-tocol toward effective catalysts for glycerol hydrogenolysis. 展开更多
关键词 Glycerol hydrogenolysis 1 3-PROPANEDIOL Metal-support interaction Second promoter Liquid phase Catalyst deactivation
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Enhanced extracellular production of alpha-lactalbumin from Bacillus subtilis through signal peptide and promoter screening
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作者 Yuqi Zhu Pengdong Sun +6 位作者 Chunjian Li Yu Zhang Yu Wang Jingyuan Li Yanfeng Liu Jian Chen Yang Deng 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第4期2310-2316,共7页
Alpha-lactalbumin(α-LA)is a major whey protein found in breast milk and plays a crucial role in the growth and development of infants.In this study,Bacillus subtilis RIK1285 harboring AprE signal peptide(SP)was selec... Alpha-lactalbumin(α-LA)is a major whey protein found in breast milk and plays a crucial role in the growth and development of infants.In this study,Bacillus subtilis RIK1285 harboring AprE signal peptide(SP)was selected as the original strain for the production ofα-LA.It was found thatα-LA was identified in the pellet after ultrasonic disruption and centrifugation instead of in the fermentation supernatant.The original strain most likely only producedα-LA intracellular,but not extracellular.To improve the expression and secretion ofα-LA in RIK1285,a library of 173 homologous SPs from the B.subtilis 168 genome was fused with target LALBA gene in the pBE-S vector and expressed extracellularly in RIK1285.SP YjcN was determined to be the best signal peptide.Bands in supernatant were observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and purified by nickel column to calculate the highest yield signal peptide.In addition,different promoters(P_(aprE),P_(43),and P_(glv))were compared and applied.The results indicated that the strain RIK1285-pBE-P_(glv)-YjcN-LALBA had the highestα-LA yield,reaching 122.04μg/mL.This study demonstrates successful expression and secretion of humanα-LA in B.subtilis and establishes a foundation for simulating breast milk for infant formulas and developing bioengineered milk. 展开更多
关键词 Bacillus subtilis ALPHA-LACTALBUMIN Bioengineering milk Signal peptide Promoter screening
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Revisiting the mitigation of coke formation:Synergism between support&promoters'role toward robust yield in the CO_(2)reformation of methane
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作者 Zahra Taherian Vahid Shahed Gharahshiran +3 位作者 Xiaoxuan Wei Alireza Khataee Yeojoon Yoon Yasin Orooji 《Nano Materials Science》 EI CAS CSCD 2024年第5期536-547,共12页
CO_(2)reformation of methane(CRM)and CO_(2)methanation are two interconnected processes with significant implications for greenhouse gas reduction and sustainable energy production for industrial purposes.While Nibase... CO_(2)reformation of methane(CRM)and CO_(2)methanation are two interconnected processes with significant implications for greenhouse gas reduction and sustainable energy production for industrial purposes.While Nibased catalysis suffers from poor stability due to coke formation or sintering,we report a super stable remedy.The active sites of mesoporous MgO were loaded using wet impregnation.The incorporation of Ni and promoters altered the physical features of the catalysts.Sm–Ni/MgO showed the smallest crystallite size,specific surface area,and pore volume.The Sm–Ni/MgO catalyst was selected as the most suitable candidate for CRM,with 82%CH4 and H2/CO ratio of approximately 100%and also for CO_(2)methanation with the conversion of carbon dioxide(82%)and the selectivity toward methane reaches 100%at temperatures above 300ᵒC.Furthermore,the Sm–Ni/MgO catalyst was stable for 900 min of continuous reaction,without significant carbon deposition.This stability was largely due to the high oxygen mobility on the catalyst surface in the presence of Sm.Overall,we demonstrated the efficacy of using promoted Ni catalysts supported by mesoporous magnesia for the improved reformation of greenhouse gases. 展开更多
关键词 Mesoporous MgO Dry reforming CO_(2)methanation Oxygen vacancies PROMOTER
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Development of biofertilizers for sustainable agriculture over four decades(1980-2022)
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作者 Guangxu Zhao Xiaoling Zhu +8 位作者 Gang Zheng Guangfan Meng Ziliang Dong Ju Hye Baek Che Ok Jeon Yanlai Yao Yuan Hu Xuan Jie Zhang Baolei Jia 《Geography and Sustainability》 CSCD 2024年第1期19-28,共10页
The application of biofertilizers is becoming an inevitable trend to substitute chemical fertilizers for sustainable agriculture.To better understand the development of biofertilizers from 1980 to 2022,we used bibliom... The application of biofertilizers is becoming an inevitable trend to substitute chemical fertilizers for sustainable agriculture.To better understand the development of biofertilizers from 1980 to 2022,we used bibliometric mining to analyze 12,880 journal articles related to biofertilizer.The network cooccurrence analysis suggested that the biofertilizers research can be separated into three stages.The first stage(1980-2005)focused on nitrogen fixation.The second stage(2006-2015)concentrated on the mechanisms for increasing plant yield.The third stage(2016-2022)was the application of biofertilizers to improve the soil environment.The keyword analysis revealed the mechanisms of biofertilizers to improve plant-growth:biofertilizers can impact the nutritional status of plants,regulate plant hormones,and improve soil environments and the microbiome.The bacteria use as biofertilizers,included Pseudomonas,Azospirillum,and Bacillus,were also identified through bibliometric mining.These findings provide critical discernment to aid further study of biofertilizers for sustainable agriculture. 展开更多
关键词 BIOFERTILIZER Plant-growth-promoting bacteria Plant-growth promoting rhizobacteria BIBLIOMETRIC Research hotspots
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孔令义/夏元铮/王凯波团队在Advanced Science发表中药活性成分黄连碱稳定ATF4启动子G-四链体克服癌症持久性细胞的最新研究成果
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作者 《中国药科大学学报》 CAS CSCD 北大核心 2024年第4期442-442,共1页
7月12日,我校孔令义(本刊副主编)/夏元铮/王凯波团队在学科顶尖期刊Advanced Science在线发表题为“Overcoming Cancer Persister Cells by Stabilizing the ATF4 Promoter G-quadruplex”的最新研究成果。我校2021级博士生肖成梅。
关键词 黄连碱 最新研究成果 王凯波 中药活性成分 PROMOTER 持久性
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Molecular cloning,characterization and promoter analysis of LbgCWIN1 and its expression profiles in response to exogenous sucrose during in vitro bulblet initiation in lily
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作者 Cong Gao Shiqi Li +4 位作者 Yunchen Xu Yue Liu Yiping Xia Ziming Ren Yun Wu 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第2期545-555,共11页
Lily(Lilium spp.) is an important ornamental flower, which is mainly propagated by bulbs. Cell wall invertases(CWINs), which catalyze the irreversibly conversion of sucrose into glucose and fructose in the extracellul... Lily(Lilium spp.) is an important ornamental flower, which is mainly propagated by bulbs. Cell wall invertases(CWINs), which catalyze the irreversibly conversion of sucrose into glucose and fructose in the extracellular space, are key enzymes participating in sucrose allocation in higher plants. Previous studies have shown that CWINs play an essential role in bulblet initiation process in bulbous crops, but the underlying molecular mechanism remains unclear. Here, a CWIN gene of Lilium brownii var. giganteum(Lbg) was identified and amplified from genomic DNA. Quantitative RT-PCR assays revealed that the expression level of LbgCWIN1 was highly upregulated exactly when the endogenous starch degraded in non-sucrose medium during in vitro bulblet initiation in Lbg. Phylogenetic relationship, motif, and domain analysis of LbgCWIN1 protein and CWINs in other plant species showed that all sequences of these CWIN proteins were highly conserved. The promoter sequence of LbgCWIN1 possessed a number of alpha-amylase-, phytohormone-, light-and stress-responsive cis-elements. Meanwhile, β-glucuronidase(GUS) assay showed that the 459 bp upstream fragment from the translational start site displayed maximal promoter activity. These results revealed that LbgCWIN1 might function in the process of in vitro bulblet initiation and be in the response to degradation of endogenous starch. 展开更多
关键词 Lilium brownii var.giganteum LbgCWIN1 Phylogenetic analysis Promoter analysis Bulblet initiation
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Contemporary strategies and approaches for characterizing composition and enhancing biofilm penetration targeting bacterial extracellular polymeric substances
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作者 Lan Lu Yuting Zhao +4 位作者 Mingxing Li Xiaobo Wang Jie Zhu Li Liao Jingya Wang 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2024年第4期506-524,共19页
Extracellular polymeric substances(EPS)constitutes crucial elements within bacterial biofilms,facili-tating accelerated antimicrobial resistance and conferring defense against the host's immune cells.Developing pr... Extracellular polymeric substances(EPS)constitutes crucial elements within bacterial biofilms,facili-tating accelerated antimicrobial resistance and conferring defense against the host's immune cells.Developing precise and effective antibiofilm approaches and strategies,tailored to the specific charac-teristics of EPS composition,can offer valuable insights for the creation of novel antimicrobial drugs.This,in turn,holds the potential to mitigate the alarming issue of bacterial drug resistance.Current analysis of EPS compositions relies heavily on colorimetric approaches with a significant bias,which is likely due to the selection of a standard compound and the cross-interference of various EPS compounds.Considering the pivotal role of EPS in biofilm functionality,it is imperative for EPS research to delve deeper into the analysis of intricate compositions,moving beyond the current focus on polymeric materials.This ne-cessitates a shift from heavy reliance on colorimetric analytic methods to more comprehensive and nuanced analytical approaches.In this study,we have provided a comprehensive summary of existing analytical methods utilized in the characterization of EPS compositions.Additionally,novel strategies aimed at targeting EPS to enhance biofilm penetration were explored,with a specific focus on high-lighting the limitations associated with colorimetric methods.Furthermore,we have outlined the challenges faced in identifying additional components of EPS and propose a prospective research plan to address these challenges.This review has the potential to guide future researchers in the search for novel compounds capable of suppressing EPS,thereby inhibiting biofilm formation.This insight opens up a new avenue for exploration within this research domain. 展开更多
关键词 Analytic strategies and approaches Composition characterization Extracellular polymeric substances(EPS) Promoting biofilm penetration
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Landscape of Sequence Variations in Homologous Copies of FAD2 and FAD3 in Rapeseed(Brassica napus L.)Germplasm with High/Low Linolenic Acid Trait
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作者 Haoxue Wu Xiaohan Zhang +5 位作者 Xiaoyu Chen Kang Li Aixia Xu Zhen Huang Jungang Dong Chengyu Yu 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第3期627-640,共14页
Genetic manipulation(either restraint or enhancement)of the biosynthesis pathway ofα-linolenic acid(ALA)in seed oil is an important goal in Brassica napus breeding.B.napus is a tetraploid plant whose genome often har... Genetic manipulation(either restraint or enhancement)of the biosynthesis pathway ofα-linolenic acid(ALA)in seed oil is an important goal in Brassica napus breeding.B.napus is a tetraploid plant whose genome often har-bors four and six homologous copies,respectively,of the two fatty acid desaturases FAD2 and FAD3,which con-trol the last two steps of ALA biosynthesis during seed oil accumulation.In this study,we compared their promoters,coding sequences,and expression levels in three high-ALA inbred lines 2006L,R8Q10,and YH25005,a low-ALA line A28,a low-ALA/high-oleic-acid accession SW,and the wildtype ZS11.The expression levels of most FAD2 and FAD3 homologs in the three high-ALA accessions were higher than those in ZS11 and much higher than those in A28 and SW.The three high-ALA accessions shared similar sequences with the pro-moters and CDSs of BnFAD3.C4 and BnFAD3.A3.In A28 and SW,substitution of three amino acid residues in BnFAD2.A5 and BnFAD2.C5,an absence of BnFAD2.C1 locus,and a 549 bp long deletion on the BnFAD3.A3 promoter were detected.The profile of BnFAD2 mutation in the two low-ALA accessions A28 and SW is different from that reported in previous studies.The mutations in BnFAD3 in the high-ALA accessions are reported for thefirst time.In identifying the sites of these mutations,we provide detailed information to aid the design of mole-cular markers for accelerated breeding schemes. 展开更多
关键词 Brassica napus linolenic acid FAD2 FAD3 promoter coding sequences mutation
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Unstable evolution of railway slope under the rainfall-vibration joint action
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作者 DONG Haoyu WANG Jiading +2 位作者 ZHANG Dengfei LI Lin XU Yuanjun 《Journal of Mountain Science》 SCIE CSCD 2024年第4期1419-1431,共13页
Understanding the unstable evolution of railway slopes is the premise for preventing slope failure and ensuring the safe operation of trains.However,as two major factors affecting the stability of railway slopes,few s... Understanding the unstable evolution of railway slopes is the premise for preventing slope failure and ensuring the safe operation of trains.However,as two major factors affecting the stability of railway slopes,few scholars have explored the unstable evolution of railway slopes under the joint action of rainfall-vibration.Based on the model test of sandy soil slope,the unstable evolution process of slope under locomotive vibration,rainfall,and rainfall-vibration joint action conditions was simulated in this paper.By comparing and analyzing the variation trends of soil pressure and water content of slope under these conditions,the change laws of pore pressure under the influence of vibration and rainfall were explored.The main control factors affecting the stability of slope structure under the joint action conditions were further defined.Combined with the slope failure phenomena under these three conditions,the causes of slope instability resulting from each leading factor were clarified.Finally,according to the above conclusions,the unstable evolution of the slope under the rainfall-vibration joint action was determined.The test results show that the unstable evolution process of sandy soil slope,under the rainfall-vibration joint action,can be divided into:rainfall erosion cracking,vibration promotion penetrating,and slope instability sliding three stages.In the process of slope unstable evolution,rainfall and vibration play the roles of inducing and promoting slide respectively.In addition,the deep cracks,which are the premise for the formation of the sliding surface,and the violent irregular fluctuation of soil pressure,which reflects the near penetration of the sliding surface,constitute the instability characteristics of the railway slope together.This paper reveals the unstable evolution of sandy soil slopes under the joint action of rainfall-vibration,hoping to provide the theoretical basis for the early warning and prevention technology of railway slopes. 展开更多
关键词 Rainfall vibration joint action Small scale model tests Unstable evolution process Unstable characteristics Inducing sliding and promoting sliding
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Shaping global health promotion:a comprehensive analysis of the 10 Global Conferences on Health Promotion Conferences(1986-2021)
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作者 Shiyu Liu Meichen Sun +4 位作者 Na Zhang Zhaozhang Sun Xiangyang Tian Liming Li Youfa Wang 《Global Health Journal》 2024年第2期91-96,共6页
Objective This study analyzed how the 10 Global Conferences on Health Promotion have played a significant role in shaping and promoting a worldwide consensus and actions on health promotion,effectively addressing dive... Objective This study analyzed how the 10 Global Conferences on Health Promotion have played a significant role in shaping and promoting a worldwide consensus and actions on health promotion,effectively addressing diverse health challenges that evolved over different periods.Methods The textual analysis method was used in this study and text encoding was conducted to systematically examine the declarations and reports presented by the 10 Global Conferences on Health Promotion held during 1986-2021.We summarized the themes and key achievements,and key vocabulary in the conference declarations was extracted and analyzed to construct the global health promotion consensus and actions.Results The fundamental principles of the conferences are to foster consensus and initiate actions in the realm of health promotion on a global scale.The primary purpose and goal are to promote health from regional to global.Significantly,our findings highlight a transition in the primary actors driving health promotion.It underscores a shift in health promotion from being driven primarily by organizations like the World Health Organization,governments,and international bodies,to a more inclusive approach involving non-governmental organizations and the general public.This development implies that health promotion has evolved into a collective global endeavor,demanding the proactive involvement of various stakeholders,and forging new alliances in public health.Meanwhile,the coronavirus disease 2019(COVID-19)pandemic has further shaped the landscape of health promotion,underscoring the need for intensified focus on areas including disease prevention,health education,and the integration of digital health technologies,and emphasizing the importance of a multidimensional,responsive approach in public health initiatives.Conclusions Sustained collaboration and innovative strategies are pivotal to advancing health promotion globally.Countries,together with public and private entities,should intensify cooperation.Multisectoral collaboration among partners such as healthcare,education,social security,and the industry is vital for health promotion and achieving global health goals. 展开更多
关键词 Global conference on health promotion Health promotion Consensus and actions
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Spatiotemporal variations,influencing factors,and configurational promotion paths of energy efficiency in China’s cities
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作者 Ruyu Xu Keliang Wang +1 位作者 Zhuang Miao Lingxuan Sun 《Chinese Journal of Population,Resources and Environment》 2024年第1期74-86,共13页
The promotion of energy efficiency(EE)helps address energy constraints and promote environmental sustainability.This study comprehensively explores the spatiotemporal variations,influencing factors,and configuration p... The promotion of energy efficiency(EE)helps address energy constraints and promote environmental sustainability.This study comprehensively explores the spatiotemporal variations,influencing factors,and configuration promotion paths of EE in 284 Chinese cities during 2003‒2019 using the global super-efficiency minimum distance to strong efficient frontier(G-S-MinDS),exploratory spatial data analysis(ESDA),multiscale geographically weighted regression(MGWR),and fuzzy set qualitative comparative analysis(fsQCA)methods.The findings are:①China’s cities have an annual average EE of 0.658 with a growth rate of 0.53%,showing considerable promotion potential.②Industrial structure optimization,population agglomeration,economic development,and increased green coverage contribute positively,while government intervention and openness hinder China’s urban EE.③Four configurational promotion paths for enhancing China’s urban EE are identified,where among those paths population density is a core condition,while government intervention is not.This study provides valuable insights into substantially improving urban EE,emphasizing the need for targeted policies to address energy and environmental crises in China. 展开更多
关键词 Energy efficiency Spatiotemporal variations Influencing factors Configurational promotion paths
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Research Progress on the Growth-Promoting Effect of Plant Biostimulants on Crops
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作者 Qi Lu Longfei Jin +3 位作者 Cuiling Tong Feng Liu Bei Huang Dejian Zhang 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第4期661-679,共19页
A Plant Biostimulant is any substance or microorganism applied to plants to enhance nutrition efficiency,abioticstress tolerance,and/or crop quality traits,regardless of its nutrient content.The application of Plant b... A Plant Biostimulant is any substance or microorganism applied to plants to enhance nutrition efficiency,abioticstress tolerance,and/or crop quality traits,regardless of its nutrient content.The application of Plant biostimulants(PBs)in production can reduce the application of traditional pesticides and chemical fertilizers and improvethe quality and yield of crops,which is conducive to the sustainable development of agriculture.An in-depthunderstanding of the mechanism and effect of various PBs is very important for how to apply PBs reasonablyand effectively in the practice of crop production.This paper summarizes the main classification of PBs;Thegrowth promotion mechanism of PBs was analyzed from four aspects:improving soil physical and chemical properties,enhancing crop nutrient absorption capacity,photosynthesis capacity,and abiotic stress tolerance;At thesame time,the effects of PBs application on seed germination,seedling vigor,crop yield,and quality were summarized;Finally,how to continue to explore and study the use and mechanism of PBs in the future is analyzedand prospected,to better guide the application of PBs in crop production in the future. 展开更多
关键词 Plant biostimulants growth promoting effect crop production
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