Objective: To investigate whether the Bc1-2 antisense oligonucleotide(ASODN) may enhance radiation-induced apoptosis in Raji cell line. Methods: Cell surviving fraction was determined using the trypan blue dye exclusi...Objective: To investigate whether the Bc1-2 antisense oligonucleotide(ASODN) may enhance radiation-induced apoptosis in Raji cell line. Methods: Cell surviving fraction was determined using the trypan blue dye exclusion assay. The expression level of bc1-2 protein was assayed by immunofluorescence using fluoresce isothiocyanate label. Apoptosis was detected by Giemsa staining and flow cytomertric cell cycle analysis. Results: It was found that Bc1-2 ASODN combined with radiation had significantly reduced the number of viable cells (P<0.05). There was no difference on cell survival between mismatch Bc1-2 oligodeoxynucleotide/radiation combination and radiation-treated cells alone. Bc1-2 ASODN combined with radiation could significantly inhibit expression of Bc1-2 protein in Raji cells (P<0.05). Cells treated with Bc1-2 ASODN combined with radiation at 72 h displayed classic apoptotic changes. Apoptosis rates of Raji cells treated with Bc1-2 oligodeoxynucleotide/radiation combination and radiation-treated cells alone, respectively. Conclusion: Bc1-2 antisense oligonucleotide can enhance radiation-induced apoptosis in Raji cell line.展开更多
Objective To study the effects of P53, PCNA, Bc1-2 protein and their relationship in salivary adenoid cystic carclnoma(SACC). Methods These protelns were examlned by lmmunohistochemistry. Results overexpressions of Ps...Objective To study the effects of P53, PCNA, Bc1-2 protein and their relationship in salivary adenoid cystic carclnoma(SACC). Methods These protelns were examlned by lmmunohistochemistry. Results overexpressions of Ps, and PCNA were revealed in ACC samples, they were higher than those in (polymorphous adenomas) PA, but expression of Bc1-2 protein was not different between ACC and PA. In 3 subtypes of ACC, expressions of 3 proteins were different. Conciusion Mutations of P53, Bc1-2 may be involed in the occurrence of SACC, expression of PCNA and mutation of P53 may coexist in the development of the SACC.展开更多
Objective: To investigate the expression of E2F and Bc1-2 and the clinicopathological significance in hepatocellular carcinoma. Methods: The expressions of E2F-3 and Bc1-2 in 74 patients with hepatic carcinoma, paraca...Objective: To investigate the expression of E2F and Bc1-2 and the clinicopathological significance in hepatocellular carcinoma. Methods: The expressions of E2F-3 and Bc1-2 in 74 patients with hepatic carcinoma, paracarcinoma and 15 patients with liver cirrhosis were detected by S-P immunohistochemical staining. Results: The expression of E2F in hepatic carcinoma was significantly higher than that in paracarcinoma or liver cirrhosis (P<0.005), the expression of Bc1-2 in hepatic carcinoma was significantly higher than that in paracarcinoma (P<0.005), in which Bc1-2 expression was lower than in liver cirrhosis(P<0.05). The expression of E2F-3 was related with histological grade, tumor size, and the expression of Bc1-2 was related with histological grade, tumor size and tumor number. There was correlation between the expression of E2F-3 and Bc1-2 in hepatic carcinoma. Conclusion: E2F-3 and Bc1-2 expression may play an important role in development, progression and cell apoptosis of tumor.展开更多
基金this work was supported by the grants from The Natural Science Program Foundation of Gaungdong Province(No.021195) and The Guangzhou City Key Foundation of Science and Technology Program (No.2001-Z- 037-01).
文摘Objective: To investigate whether the Bc1-2 antisense oligonucleotide(ASODN) may enhance radiation-induced apoptosis in Raji cell line. Methods: Cell surviving fraction was determined using the trypan blue dye exclusion assay. The expression level of bc1-2 protein was assayed by immunofluorescence using fluoresce isothiocyanate label. Apoptosis was detected by Giemsa staining and flow cytomertric cell cycle analysis. Results: It was found that Bc1-2 ASODN combined with radiation had significantly reduced the number of viable cells (P<0.05). There was no difference on cell survival between mismatch Bc1-2 oligodeoxynucleotide/radiation combination and radiation-treated cells alone. Bc1-2 ASODN combined with radiation could significantly inhibit expression of Bc1-2 protein in Raji cells (P<0.05). Cells treated with Bc1-2 ASODN combined with radiation at 72 h displayed classic apoptotic changes. Apoptosis rates of Raji cells treated with Bc1-2 oligodeoxynucleotide/radiation combination and radiation-treated cells alone, respectively. Conclusion: Bc1-2 antisense oligonucleotide can enhance radiation-induced apoptosis in Raji cell line.
文摘Objective To study the effects of P53, PCNA, Bc1-2 protein and their relationship in salivary adenoid cystic carclnoma(SACC). Methods These protelns were examlned by lmmunohistochemistry. Results overexpressions of Ps, and PCNA were revealed in ACC samples, they were higher than those in (polymorphous adenomas) PA, but expression of Bc1-2 protein was not different between ACC and PA. In 3 subtypes of ACC, expressions of 3 proteins were different. Conciusion Mutations of P53, Bc1-2 may be involed in the occurrence of SACC, expression of PCNA and mutation of P53 may coexist in the development of the SACC.
文摘Objective: To investigate the expression of E2F and Bc1-2 and the clinicopathological significance in hepatocellular carcinoma. Methods: The expressions of E2F-3 and Bc1-2 in 74 patients with hepatic carcinoma, paracarcinoma and 15 patients with liver cirrhosis were detected by S-P immunohistochemical staining. Results: The expression of E2F in hepatic carcinoma was significantly higher than that in paracarcinoma or liver cirrhosis (P<0.005), the expression of Bc1-2 in hepatic carcinoma was significantly higher than that in paracarcinoma (P<0.005), in which Bc1-2 expression was lower than in liver cirrhosis(P<0.05). The expression of E2F-3 was related with histological grade, tumor size, and the expression of Bc1-2 was related with histological grade, tumor size and tumor number. There was correlation between the expression of E2F-3 and Bc1-2 in hepatic carcinoma. Conclusion: E2F-3 and Bc1-2 expression may play an important role in development, progression and cell apoptosis of tumor.
