Objective To research a protein chip method which can simultaneously quantitative detect β-Lactoglobulin （β-L） and Lactoferrin （Lf） at one time. Methods Protein chip printer was used to print both anti-β-L anti...Objective To research a protein chip method which can simultaneously quantitative detect β-Lactoglobulin （β-L） and Lactoferrin （Lf） at one time. Methods Protein chip printer was used to print both anti-β-L antibodies and anti-Lf antibodies on each block of protein chip. And then an improved sandwich detection method was applied while the other two detecting antibodies for the two antigens were added in the block after they were mixed. The detection conditions of the quantitative detection for simultaneous measurement of β-L and Lf with protein chip were optimized and evaluated. Based on these detected conditions, two standard curves of the two proteins were simultaneously established on one protein chip. Finally, the new detection method was evaluated by using the analysis of precision and accuracy. Results By comparison experiment, mouse monoclonal antibodies of the two antigens were chosen as the printing probe. The concentrations of β-L and Lf probes were 0.5 mg/mL and 0.5 mg/mL, respectively, while the titers of detection antibodies both of β-L and Lf were 1：2,000. Intra- and inter-assay variability was between 4.88% and 38.33% for all tests. The regression coefficients of protein chip comparing with ELISA for β-L and Lf were better than 0.734, and both of the two regression coefficients were statistically significant （r = 0.734, t = 2.644, P = 0.038; and r = 0.774, t = 2.998, P = 0.024）. Conclusion A protein chip method of simultaneously quantitative detection for β-L and Lf has been established and this method is worthy in further application.展开更多
BACKGROUND： Studies have reported the combined use of two-dimensional gel electrophoresis and mass spectrometry to detect differentially expressed proteins in the rat brainstem following brain injury. However, the de...BACKGROUND： Studies have reported the combined use of two-dimensional gel electrophoresis and mass spectrometry to detect differentially expressed proteins in the rat brainstem following brain injury. However, the detected differential proteins often exhibit low sensitivity and high relative molecular weight. Although protein chip technology is thought to compensate for these inadequacies, no related studies or results have been reported. OBJECTIVE： To propose the application of weak cation exchange protein chips in combination with mass spectrometry for determining protein expression profiles and characteristics in the brainstem following closed brain injury. DESIGN, TIME AND SETTING： Randomized, controlled, animal experiments utilizing proteomics were performed from June 2007 to December 2008 in the Proteomics Laboratory, Medical College of Chinese People＇s Armed Police Force. MATERIALS： Weak cation exchange 2 protein chip, Ciphergen Proteinchip System （PBS-IIC）. METHODS： A total of 72 rats were randomly assigned to two groups： sham-surgery （n = 12） and injury （n = 60）. A closed traumatic brain injury model caused by falling object was replicated in the injury group, which was then subdivided into five subgroups according to different time points after injury： 4, 8, 12, 24, and 48 hours, with 12 rats in each subgroup. In the sham-surgery group, only the skin was removed and the stainless steel pad was fixed to the skull. MAIN OUTCOME MEASURES： The brain injury rats were sacrificed at 4, 8, 12, 24, and 48 hours after injury, respectively, and the control rats were sacrificed at 24 hours. Pathological changes in the brainstem were determined using hematoxylin-eosin staining, and differential protein expression in the brainstem was detected using a weak cation exchange 2 protein chip and protein chip reader. RESULTS： In the sham-surgery group, cells appeared normal. However, in the brain injury group, some brainstem neurons exhibited pyknosis, with reduced numbers of Nissl bodies in the cytoplasm swollen cell bodies and nuclei, irregular staining in the cytoplasm, and decreased numbers of neurons. Results from weak cation exchange 2 protein chip detection demonstrated that, compared with the sham-surgery group, the expression profiles of 2 proteins were altered in the brainstem of the injury group. At 12, 24, and 48 hours after injury, expression was increased （P 〈 0.01 ）. The mass charge ratio （M/Z） of 7 862 differentially expressed proteins was greater in the sham-surgery group compared with 12 and 24 hours after injury （P 〈 0.05）. CONCLUSION： The combined method of weak cation exchange 2 protein chip and mass spectrometry detected differential protein expression in the brainstem following closed brain injury in the rats, which suggested that closed brain injury induced altered protein expression profiles in the brainstem.展开更多
The proteomics of the differential protein expressions in human glioma cell line U251 cells infected with human cytomegalovirus(HCMV)was investigated at the protein level by using the surface enhanced laser desorption...The proteomics of the differential protein expressions in human glioma cell line U251 cells infected with human cytomegalovirus(HCMV)was investigated at the protein level by using the surface enhanced laser desorption/ionization(SELDI)protein chip system in order to develop a method of study for the pathogenesis of HCMV infection.In this study,the cultured U251 cells were infected with HC- MV in good condition and the supernatants of lysates and the extracellular fluids of the cultivated infect- ed cells were quantitatively defined for the expressed proteins.The proteomics of the differential protein expression in cells before and after infection was analyzed by WCX2 arrays on the protein chip reader. It was demonstrated that the cytopathic effects of infected cells appeared on the 5th day after infection, however,the differential protein expression was evident at 6 h after infection as revealed by RT-PCR and mass spectrometry.The protein peaks captured from different batches of samples,from the same sample detected with different arrays or for the different limes were all equivalent.With the molecular weight range from 2000 Da to 3000 Da,chip captured 82 peaks from the intracellular fluids and 11 protein peak from the cellular fluid in which compared with the control group,the protein peaks with molecular weight of 13 536.3 Da,10 046.1 Da and 17 106.2 Da were close to those ofβ-amyloid pro- tein,caspase-1 precursor and LPS-induced TNF-αfactor respectively,which showed brief up-regulation 4 h after infection,and continued to raise 48 h later.These results infer that these proteins may be re- lated to the apoptosis induced by HCMV infection,thus suggesting that the apeptosis induced by HC- MV infection may play a role in the pathogenesis of HCMV infection.展开更多
The purpose of this study was to develop a protein chip technique based on receptor binding assays to measure bioavailable serum sex hormone levels (BSSHL). 224 aging healthy Chinese were investigated to get the refer...The purpose of this study was to develop a protein chip technique based on receptor binding assays to measure bioavailable serum sex hormone levels (BSSHL). 224 aging healthy Chinese were investigated to get the referenced values of BSSHL for the first time. In the assays recombined sex hormone receptor proteins were jointed to polysaccharide coated slides to make protein chip, and the dose-dependence curves of sex hormone on chip were prepared. The data showed that this method had good precision (CV<16%) and accuracy (Bias<10%), and the sensitivity could reach 1 pmol/L. From the results, BSSHL of men and women declined with aging, but no significant differences were observed. The BSSHL of aging men were higher than those of women. The bioavailable serum androgen level of men was 52―112 pmol/L, women's was 3―70 pmol/L and the whole group was 41.9―81.4 pmol/L. The bioavailable serum estrogen level of men was 0.8―3.0 pmol/L, women's was 1.2―2.5 pmol/L and the whole group was 0.6―2.64 pmol/L. Based on the assays, BSSHL measurement by protein chip can meet the needs of epidemiological studies in terms of speed, accuracy and sample volume required, and was helpful in quantitative assessment of aging people's health.展开更多
Neonatal hypoxic-ischemic encephalopathy is often associated with permanent cerebral palsy,neurosensory impairments,and cognitive deficits,and there is no effective treatment for complications related to hypoxic-ische...Neonatal hypoxic-ischemic encephalopathy is often associated with permanent cerebral palsy,neurosensory impairments,and cognitive deficits,and there is no effective treatment for complications related to hypoxic-ischemic encephalopathy.The therapeutic potential of human placental chorionic plate-derived mesenchymal stem cells for various diseases has been explored.However,the potential use of human placental chorionic plate-derived mesenchymal stem cells for the treatment of neonatal hypoxic-ischemic encephalopathy has not yet been investigated.In this study,we injected human placental chorionic plate-derived mesenchymal stem cells into the lateral ventricle of a neonatal hypoxic-ischemic encephalopathy rat model and observed significant improvements in both cognitive and motor function.Protein chip analysis showed that interleukin-3 expression was significantly elevated in neonatal hypoxic-ischemic encephalopathy model rats.Following transplantation of human placental chorionic plate-derived mesenchymal stem cells,interleukin-3 expression was downregulated.To further investigate the role of interleukin-3 in neonatal hypoxic-ischemic encephalopathy,we established an in vitro SH-SY5Y cell model of hypoxic-ischemic injury through oxygen-glucose deprivation and silenced interleukin-3 expression using small interfering RNA.We found that the activity and proliferation of SH-SY5Y cells subjected to oxygen-glucose deprivation were further suppressed by interleukin-3 knockdown.Furthermore,interleukin-3 knockout exacerbated neuronal damage and cognitive and motor function impairment in rat models of hypoxic-ischemic encephalopathy.The findings suggest that transplantation of hpcMSCs ameliorated behavioral impairments in a rat model of hypoxic-ischemic encephalopathy,and this effect was mediated by interleukin-3-dependent neurological function.展开更多
目的筛选出与流感病毒Non-structural protein 1(NS1)蛋白结合的人类宿主蛋白并加以分析,确定这些结合蛋白富集的方向及关键蛋白,为抗流感病毒的新药研发提供思路。方法将NS1样本、Biotin样本分别与HuProt^(TM)人类蛋白质组芯片进行杂...目的筛选出与流感病毒Non-structural protein 1(NS1)蛋白结合的人类宿主蛋白并加以分析,确定这些结合蛋白富集的方向及关键蛋白,为抗流感病毒的新药研发提供思路。方法将NS1样本、Biotin样本分别与HuProt^(TM)人类蛋白质组芯片进行杂交孵育,以两重复均满足Z-Score≥3为筛选条件对与NS1蛋白有结合的宿主蛋白进行筛选得到特异性检出蛋白,实验组(NS1蛋白)与对照组(Biotin)比值I Mean_Ratio≥1.4为条件筛选出显著特异性检出蛋白。用检出的195个蛋白进行GO(Biological Process,Molecular Function,Cellular Component)和KEGG_PATHWAY分析,通过蛋白-蛋白相互作用(PPI)及MCODE分析得到关键蛋白。结果获得显著特异性检出蛋白195个,GO分析结果显示这些蛋白主要参与了mRNA加工、RNA结合、蛋白结合,KEGG分析主要富集到RNA降解、氨基酸的生物合成等通路。得到的4个关键蛋白DDX6、HSPD1、PKLR、MTHFD1中DDX6与RNA的合成、翻译等过程相关,而NS1蛋白可以通过调控流感病毒RNA和宿主RNA促进病毒的感染,推测DDX6可能在该过程发挥作用;其他3个蛋白目前虽然没有明确的研究指明其与流感病毒有关系,但是能在其他RNA病毒的感染过程中发挥作用。结论与NS1结合的人类蛋白主要富集到RNA合成、加工、转录等过程中,MCODE分析得到的关键蛋白有潜力成为抗流感病毒新的靶点,但作用机制需要后续实验进行进一步验证。展开更多
目的探讨贝伐珠单抗靶向治疗卵巢癌对肿瘤标志物的影响。方法选取2019年1月—2021年12月福建医科大学附属福州市第一医院收治的80例卵巢癌患者,根据不同治疗药物进行分组,分别纳入单独行化疗的化疗组和行贝伐珠单抗靶向治疗的靶向组,每...目的探讨贝伐珠单抗靶向治疗卵巢癌对肿瘤标志物的影响。方法选取2019年1月—2021年12月福建医科大学附属福州市第一医院收治的80例卵巢癌患者,根据不同治疗药物进行分组,分别纳入单独行化疗的化疗组和行贝伐珠单抗靶向治疗的靶向组,每组40例。测评2组治疗前后肿瘤标志物蛋白芯片中糖链抗原125(glycoantigens in protein chips-125,CA-125)、人附睾蛋白4(Human epididymal protein 4,HE4)水平及血管内皮生长因子(vascular endothelial growth factor,VEGF)水平,治疗后客观缓解率、疾病控制率、免疫指标、癌症患者生命质量测定量表(quality of life scale for cancer patients,FACT-G)和抑郁自评量表(self-rating depression scale,SDS)、焦虑自评量表(self-rating anxiety scale,SAS)评分。结果治疗后,靶向组治疗客观缓解率为65.00%,疾病控制率为87.50%,高于化疗组的42.50%、70.00%(P<0.05);靶向组CA-125、HE4、VEGF指标低于化疗组,CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)高于化疗组,FACT-G评分高于化疗组,而SDS、SAS评分低于化疗组,差异有统计学意义(P<0.05)。结论贝伐珠单抗靶向治疗卵巢癌患者可抑制肿瘤新生血管形成和生长,增强治疗效果,改善患者的心理情绪和生活质量。展开更多
Objective： To study the expressive characteristics of SKP2 protein in lung carcinoma and its implication for prognosis. Methods： The expression of SKP2 protein was detected in 89 non small cell lung carcinoma, 13 sm...Objective： To study the expressive characteristics of SKP2 protein in lung carcinoma and its implication for prognosis. Methods： The expression of SKP2 protein was detected in 89 non small cell lung carcinoma, 13 small cell lung carcinoma, 10 lung benign lesion tissues by Tissue Chip and Immunohistochemistry technology. Results： The positive rate of SKP2 protein staining was （23.52±13.57）% in non small cell lung carcinoma and （53.85±12.26）% in small cell lung carcinoma, which were significantly higher than （2.91±1.27）% in lung benign lesion tissues. It was highest in small cell lung carcinoma and lowest in lung benign lesion tissues, with a significant difference between them （P=0.000）. The expressive level of SKP2 protein in lung carcinoma tissues was closely related to cell differentiation, lymph node metastasis and pathological types, but not to age, sex, smoking history, tumor site and size, and TNM staging. The survival analysis revealed that the 5-year survival rate of lung carcinoma patients was lower in SKP2 protein positive expression group than that in negative expression group （P1=0.003/0.002; r=-0.275, P2=0.005）. Conclusion. The positive expression of SKP2 protein is higher in lung carcinoma than in lung benign lesion tissues, in particular, much higher in small cell lung carcinoma. In lung carcinoma, its expressive level was closely related to cell differentiation, lymph node metastasis and pathological types. Moreover, it may be an independent factor to prognosis of patients with lung carcinoma.展开更多
染色体免疫共沉淀测序(Chromatin immunoprecipitation followed by sequencing,ChIP-seq)是研究DNA-蛋白质互作的有力工具,被广泛用于RNA聚合酶、转录因子和组蛋白修饰等在基因组上的精确定位。近年来,在ChIP-seq技术的基础上,科学家...染色体免疫共沉淀测序(Chromatin immunoprecipitation followed by sequencing,ChIP-seq)是研究DNA-蛋白质互作的有力工具,被广泛用于RNA聚合酶、转录因子和组蛋白修饰等在基因组上的精确定位。近年来,在ChIP-seq技术的基础上,科学家提出了一系列研究DNA-蛋白质互作的技术方法,提高了测序分辨率,降低了实验成本,极大推动了表观基因组学的发展。本文综述了多种DNA-蛋白质互作研究技术的原理及其应用场景,介绍了在单细胞水平上研究DNA-蛋白质互作的实现方法,并展望其未来发展的方向。展开更多
基金Sponsored by the Young Scholar Scientific Research Foundation of China CDC[2015A202]:The establishment of testing platform of quantitatively detecting main protein of cow milk by using protein chip technique
文摘Objective To research a protein chip method which can simultaneously quantitative detect β-Lactoglobulin （β-L） and Lactoferrin （Lf） at one time. Methods Protein chip printer was used to print both anti-β-L antibodies and anti-Lf antibodies on each block of protein chip. And then an improved sandwich detection method was applied while the other two detecting antibodies for the two antigens were added in the block after they were mixed. The detection conditions of the quantitative detection for simultaneous measurement of β-L and Lf with protein chip were optimized and evaluated. Based on these detected conditions, two standard curves of the two proteins were simultaneously established on one protein chip. Finally, the new detection method was evaluated by using the analysis of precision and accuracy. Results By comparison experiment, mouse monoclonal antibodies of the two antigens were chosen as the printing probe. The concentrations of β-L and Lf probes were 0.5 mg/mL and 0.5 mg/mL, respectively, while the titers of detection antibodies both of β-L and Lf were 1：2,000. Intra- and inter-assay variability was between 4.88% and 38.33% for all tests. The regression coefficients of protein chip comparing with ELISA for β-L and Lf were better than 0.734, and both of the two regression coefficients were statistically significant （r = 0.734, t = 2.644, P = 0.038; and r = 0.774, t = 2.998, P = 0.024）. Conclusion A protein chip method of simultaneously quantitative detection for β-L and Lf has been established and this method is worthy in further application.
基金the National Natural Science Foundation of China, No.30471934
文摘BACKGROUND： Studies have reported the combined use of two-dimensional gel electrophoresis and mass spectrometry to detect differentially expressed proteins in the rat brainstem following brain injury. However, the detected differential proteins often exhibit low sensitivity and high relative molecular weight. Although protein chip technology is thought to compensate for these inadequacies, no related studies or results have been reported. OBJECTIVE： To propose the application of weak cation exchange protein chips in combination with mass spectrometry for determining protein expression profiles and characteristics in the brainstem following closed brain injury. DESIGN, TIME AND SETTING： Randomized, controlled, animal experiments utilizing proteomics were performed from June 2007 to December 2008 in the Proteomics Laboratory, Medical College of Chinese People＇s Armed Police Force. MATERIALS： Weak cation exchange 2 protein chip, Ciphergen Proteinchip System （PBS-IIC）. METHODS： A total of 72 rats were randomly assigned to two groups： sham-surgery （n = 12） and injury （n = 60）. A closed traumatic brain injury model caused by falling object was replicated in the injury group, which was then subdivided into five subgroups according to different time points after injury： 4, 8, 12, 24, and 48 hours, with 12 rats in each subgroup. In the sham-surgery group, only the skin was removed and the stainless steel pad was fixed to the skull. MAIN OUTCOME MEASURES： The brain injury rats were sacrificed at 4, 8, 12, 24, and 48 hours after injury, respectively, and the control rats were sacrificed at 24 hours. Pathological changes in the brainstem were determined using hematoxylin-eosin staining, and differential protein expression in the brainstem was detected using a weak cation exchange 2 protein chip and protein chip reader. RESULTS： In the sham-surgery group, cells appeared normal. However, in the brain injury group, some brainstem neurons exhibited pyknosis, with reduced numbers of Nissl bodies in the cytoplasm swollen cell bodies and nuclei, irregular staining in the cytoplasm, and decreased numbers of neurons. Results from weak cation exchange 2 protein chip detection demonstrated that, compared with the sham-surgery group, the expression profiles of 2 proteins were altered in the brainstem of the injury group. At 12, 24, and 48 hours after injury, expression was increased （P 〈 0.01 ）. The mass charge ratio （M/Z） of 7 862 differentially expressed proteins was greater in the sham-surgery group compared with 12 and 24 hours after injury （P 〈 0.05）. CONCLUSION： The combined method of weak cation exchange 2 protein chip and mass spectrometry detected differential protein expression in the brainstem following closed brain injury in the rats, which suggested that closed brain injury induced altered protein expression profiles in the brainstem.
基金This work was supported by National Natural Science Foundation of China(No.30471527 and No.30540075)partly supported by Mr.Tai Scholar Construction Engineering Foundation.
文摘The proteomics of the differential protein expressions in human glioma cell line U251 cells infected with human cytomegalovirus(HCMV)was investigated at the protein level by using the surface enhanced laser desorption/ionization(SELDI)protein chip system in order to develop a method of study for the pathogenesis of HCMV infection.In this study,the cultured U251 cells were infected with HC- MV in good condition and the supernatants of lysates and the extracellular fluids of the cultivated infect- ed cells were quantitatively defined for the expressed proteins.The proteomics of the differential protein expression in cells before and after infection was analyzed by WCX2 arrays on the protein chip reader. It was demonstrated that the cytopathic effects of infected cells appeared on the 5th day after infection, however,the differential protein expression was evident at 6 h after infection as revealed by RT-PCR and mass spectrometry.The protein peaks captured from different batches of samples,from the same sample detected with different arrays or for the different limes were all equivalent.With the molecular weight range from 2000 Da to 3000 Da,chip captured 82 peaks from the intracellular fluids and 11 protein peak from the cellular fluid in which compared with the control group,the protein peaks with molecular weight of 13 536.3 Da,10 046.1 Da and 17 106.2 Da were close to those ofβ-amyloid pro- tein,caspase-1 precursor and LPS-induced TNF-αfactor respectively,which showed brief up-regulation 4 h after infection,and continued to raise 48 h later.These results infer that these proteins may be re- lated to the apoptosis induced by HCMV infection,thus suggesting that the apeptosis induced by HC- MV infection may play a role in the pathogenesis of HCMV infection.
文摘The purpose of this study was to develop a protein chip technique based on receptor binding assays to measure bioavailable serum sex hormone levels (BSSHL). 224 aging healthy Chinese were investigated to get the referenced values of BSSHL for the first time. In the assays recombined sex hormone receptor proteins were jointed to polysaccharide coated slides to make protein chip, and the dose-dependence curves of sex hormone on chip were prepared. The data showed that this method had good precision (CV<16%) and accuracy (Bias<10%), and the sensitivity could reach 1 pmol/L. From the results, BSSHL of men and women declined with aging, but no significant differences were observed. The BSSHL of aging men were higher than those of women. The bioavailable serum androgen level of men was 52―112 pmol/L, women's was 3―70 pmol/L and the whole group was 41.9―81.4 pmol/L. The bioavailable serum estrogen level of men was 0.8―3.0 pmol/L, women's was 1.2―2.5 pmol/L and the whole group was 0.6―2.64 pmol/L. Based on the assays, BSSHL measurement by protein chip can meet the needs of epidemiological studies in terms of speed, accuracy and sample volume required, and was helpful in quantitative assessment of aging people's health.
基金supported by the National Natural Science Foundation of China,No.82001604Guizhou Provincial Higher Education Science and Technology Innovation Team,No.[2023]072+1 种基金Guizhou Province Distinguished Young Scientific and Technological Talent Program,No.YQK[2023]040Guizhou Provincial Basic Research Program(Natural Science),No.ZK[2021]-368(all to LXiong),and Zunyi City Innovative Talent Team Training Plan,No.[2022]-2.
文摘Neonatal hypoxic-ischemic encephalopathy is often associated with permanent cerebral palsy,neurosensory impairments,and cognitive deficits,and there is no effective treatment for complications related to hypoxic-ischemic encephalopathy.The therapeutic potential of human placental chorionic plate-derived mesenchymal stem cells for various diseases has been explored.However,the potential use of human placental chorionic plate-derived mesenchymal stem cells for the treatment of neonatal hypoxic-ischemic encephalopathy has not yet been investigated.In this study,we injected human placental chorionic plate-derived mesenchymal stem cells into the lateral ventricle of a neonatal hypoxic-ischemic encephalopathy rat model and observed significant improvements in both cognitive and motor function.Protein chip analysis showed that interleukin-3 expression was significantly elevated in neonatal hypoxic-ischemic encephalopathy model rats.Following transplantation of human placental chorionic plate-derived mesenchymal stem cells,interleukin-3 expression was downregulated.To further investigate the role of interleukin-3 in neonatal hypoxic-ischemic encephalopathy,we established an in vitro SH-SY5Y cell model of hypoxic-ischemic injury through oxygen-glucose deprivation and silenced interleukin-3 expression using small interfering RNA.We found that the activity and proliferation of SH-SY5Y cells subjected to oxygen-glucose deprivation were further suppressed by interleukin-3 knockdown.Furthermore,interleukin-3 knockout exacerbated neuronal damage and cognitive and motor function impairment in rat models of hypoxic-ischemic encephalopathy.The findings suggest that transplantation of hpcMSCs ameliorated behavioral impairments in a rat model of hypoxic-ischemic encephalopathy,and this effect was mediated by interleukin-3-dependent neurological function.
文摘目的探讨贝伐珠单抗靶向治疗卵巢癌对肿瘤标志物的影响。方法选取2019年1月—2021年12月福建医科大学附属福州市第一医院收治的80例卵巢癌患者,根据不同治疗药物进行分组,分别纳入单独行化疗的化疗组和行贝伐珠单抗靶向治疗的靶向组,每组40例。测评2组治疗前后肿瘤标志物蛋白芯片中糖链抗原125(glycoantigens in protein chips-125,CA-125)、人附睾蛋白4(Human epididymal protein 4,HE4)水平及血管内皮生长因子(vascular endothelial growth factor,VEGF)水平,治疗后客观缓解率、疾病控制率、免疫指标、癌症患者生命质量测定量表(quality of life scale for cancer patients,FACT-G)和抑郁自评量表(self-rating depression scale,SDS)、焦虑自评量表(self-rating anxiety scale,SAS)评分。结果治疗后,靶向组治疗客观缓解率为65.00%,疾病控制率为87.50%,高于化疗组的42.50%、70.00%(P<0.05);靶向组CA-125、HE4、VEGF指标低于化疗组,CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)高于化疗组,FACT-G评分高于化疗组,而SDS、SAS评分低于化疗组,差异有统计学意义(P<0.05)。结论贝伐珠单抗靶向治疗卵巢癌患者可抑制肿瘤新生血管形成和生长,增强治疗效果,改善患者的心理情绪和生活质量。
文摘Objective： To study the expressive characteristics of SKP2 protein in lung carcinoma and its implication for prognosis. Methods： The expression of SKP2 protein was detected in 89 non small cell lung carcinoma, 13 small cell lung carcinoma, 10 lung benign lesion tissues by Tissue Chip and Immunohistochemistry technology. Results： The positive rate of SKP2 protein staining was （23.52±13.57）% in non small cell lung carcinoma and （53.85±12.26）% in small cell lung carcinoma, which were significantly higher than （2.91±1.27）% in lung benign lesion tissues. It was highest in small cell lung carcinoma and lowest in lung benign lesion tissues, with a significant difference between them （P=0.000）. The expressive level of SKP2 protein in lung carcinoma tissues was closely related to cell differentiation, lymph node metastasis and pathological types, but not to age, sex, smoking history, tumor site and size, and TNM staging. The survival analysis revealed that the 5-year survival rate of lung carcinoma patients was lower in SKP2 protein positive expression group than that in negative expression group （P1=0.003/0.002; r=-0.275, P2=0.005）. Conclusion. The positive expression of SKP2 protein is higher in lung carcinoma than in lung benign lesion tissues, in particular, much higher in small cell lung carcinoma. In lung carcinoma, its expressive level was closely related to cell differentiation, lymph node metastasis and pathological types. Moreover, it may be an independent factor to prognosis of patients with lung carcinoma.
文摘染色体免疫共沉淀测序(Chromatin immunoprecipitation followed by sequencing,ChIP-seq)是研究DNA-蛋白质互作的有力工具,被广泛用于RNA聚合酶、转录因子和组蛋白修饰等在基因组上的精确定位。近年来,在ChIP-seq技术的基础上,科学家提出了一系列研究DNA-蛋白质互作的技术方法,提高了测序分辨率,降低了实验成本,极大推动了表观基因组学的发展。本文综述了多种DNA-蛋白质互作研究技术的原理及其应用场景,介绍了在单细胞水平上研究DNA-蛋白质互作的实现方法,并展望其未来发展的方向。
