BACKGROUND A series of long non-coding RNAs(lncRNAs)have been reported to play a crucial role in cancer biology.Some previous studies report that lncRNA CDKN2B-AS1 is involved in some human malignancies.However,its ro...BACKGROUND A series of long non-coding RNAs(lncRNAs)have been reported to play a crucial role in cancer biology.Some previous studies report that lncRNA CDKN2B-AS1 is involved in some human malignancies.However,its role in hepatocellular carcinoma(HCC)has not been fully deciphered.AIM To decipher the role of CDKN2B-AS1 in the progression of HCC.METHODS CDKN2B-AS1 expression in HCC was detected by quantitative real-time polymerase chain reaction.The malignant phenotypes of Li-7 and SNU-182 cells were detected by the CCK-8 method,EdU method,and flow cytometry,respectively.RNA immunoprecipitation was executed to confirm the interaction between CDKN2B-AS1 and E2F transcription factor 1(E2F1).Luciferase reporter assay and chromatin immunoprecipitation were performed to verify the binding of E2F1 to the promoter of G protein subunit alpha Z(GNAZ).E2F1 and GNAZ were detected by western blot in HCC cells.RESULTS In HCC tissues,CDKN2B-AS1 was upregulated.Depletion of CDKN2B-AS1 inhibited the proliferation of HCC cells,and the depletion of CDKN2B-AS1 also induced cell cycle arrest and apoptosis.CDKN2B-AS1 could interact with E2F1.Depletion of CDKN2B-AS1 inhibited the binding of E2F1 to the GNAZ promoter region.Overexpression of E2F1 reversed the biological effects of depletion of CDKN2B-AS1 on the malignant behaviors of HCC cells.CONCLUSION CDKN2B-AS1 recruits E2F1 to facilitate GNAZ transcription to promote HCC progression.展开更多
Z-scan technique is an effective tool for measuring the optical nonlinearity of the materials. By using this technique the measurement was made for total protein and albumin. The nonlinear refractive index of the tota...Z-scan technique is an effective tool for measuring the optical nonlinearity of the materials. By using this technique the measurement was made for total protein and albumin. The nonlinear refractive index of the total protein and albumin were found to vary linearly with concentration. Hence by calculating the nonlinear refractive index it is possible to measure their concentration in the sample. The values measured thus are found in equivalence with conventional colorimetric method.展开更多
目的探讨先兆子痫患者血浆蛋白Z(PZ)水平及其内含子F G79A基因多态性的发生率与先兆子痫之间的相关性和临床意义。方法对先兆子痫组160例和正常妊娠对照组162例检测PZ水平并采用PCR和限制性内切酶片段长度多态性方法并结合基因测序检测P...目的探讨先兆子痫患者血浆蛋白Z(PZ)水平及其内含子F G79A基因多态性的发生率与先兆子痫之间的相关性和临床意义。方法对先兆子痫组160例和正常妊娠对照组162例检测PZ水平并采用PCR和限制性内切酶片段长度多态性方法并结合基因测序检测PZ内含子F G79A基因多态性,同时检测两组D-D、FDP、AT:A的水平,观察两组各项指标变化情况。结果先兆子痫组PZ水平显著低于正常妊娠对照组(1.57±0.32 mg/L vs 2.12±0.35 mg/L,P<0.05);两组人群均存在PZ内含子F G79A基因多态性。先兆子痫组GG型、GA型和AA型分别占19.38%、53.75%和26.87%,G、A等位基因频率分别为46.25%和53.75%;正常妊娠对照组GG型、GA型和AA型分别占17.90%、54.94%和27.16%,G、A等位基因频率分别为45.37%和54.63%,两组基因型和等位基因频率分布无显著差异(均P>0.05);先兆子痫组D-D、FDP水平明显高于正常对照组(5.15±1.03μg/ml vs 0.58±1.32μg/ml,P<0.05;19.46±3.28μg/ml vs 2.93±1.92μg/ml,P<0.05),而AT:A水平无显著差异(99.12±13.5%vs 100.8±10.6%,P>0.05)。结论先兆子痫患者存在PZ内含子FG79A基因多态性,但该基因多态性与疾病的发生关联不大,然而该疾病与PZ水平、D-D、FDP水平却有一定的相关性。展开更多
文摘BACKGROUND A series of long non-coding RNAs(lncRNAs)have been reported to play a crucial role in cancer biology.Some previous studies report that lncRNA CDKN2B-AS1 is involved in some human malignancies.However,its role in hepatocellular carcinoma(HCC)has not been fully deciphered.AIM To decipher the role of CDKN2B-AS1 in the progression of HCC.METHODS CDKN2B-AS1 expression in HCC was detected by quantitative real-time polymerase chain reaction.The malignant phenotypes of Li-7 and SNU-182 cells were detected by the CCK-8 method,EdU method,and flow cytometry,respectively.RNA immunoprecipitation was executed to confirm the interaction between CDKN2B-AS1 and E2F transcription factor 1(E2F1).Luciferase reporter assay and chromatin immunoprecipitation were performed to verify the binding of E2F1 to the promoter of G protein subunit alpha Z(GNAZ).E2F1 and GNAZ were detected by western blot in HCC cells.RESULTS In HCC tissues,CDKN2B-AS1 was upregulated.Depletion of CDKN2B-AS1 inhibited the proliferation of HCC cells,and the depletion of CDKN2B-AS1 also induced cell cycle arrest and apoptosis.CDKN2B-AS1 could interact with E2F1.Depletion of CDKN2B-AS1 inhibited the binding of E2F1 to the GNAZ promoter region.Overexpression of E2F1 reversed the biological effects of depletion of CDKN2B-AS1 on the malignant behaviors of HCC cells.CONCLUSION CDKN2B-AS1 recruits E2F1 to facilitate GNAZ transcription to promote HCC progression.
文摘Z-scan technique is an effective tool for measuring the optical nonlinearity of the materials. By using this technique the measurement was made for total protein and albumin. The nonlinear refractive index of the total protein and albumin were found to vary linearly with concentration. Hence by calculating the nonlinear refractive index it is possible to measure their concentration in the sample. The values measured thus are found in equivalence with conventional colorimetric method.
文摘目的探讨先兆子痫患者血浆蛋白Z(PZ)水平及其内含子F G79A基因多态性的发生率与先兆子痫之间的相关性和临床意义。方法对先兆子痫组160例和正常妊娠对照组162例检测PZ水平并采用PCR和限制性内切酶片段长度多态性方法并结合基因测序检测PZ内含子F G79A基因多态性,同时检测两组D-D、FDP、AT:A的水平,观察两组各项指标变化情况。结果先兆子痫组PZ水平显著低于正常妊娠对照组(1.57±0.32 mg/L vs 2.12±0.35 mg/L,P<0.05);两组人群均存在PZ内含子F G79A基因多态性。先兆子痫组GG型、GA型和AA型分别占19.38%、53.75%和26.87%,G、A等位基因频率分别为46.25%和53.75%;正常妊娠对照组GG型、GA型和AA型分别占17.90%、54.94%和27.16%,G、A等位基因频率分别为45.37%和54.63%,两组基因型和等位基因频率分布无显著差异(均P>0.05);先兆子痫组D-D、FDP水平明显高于正常对照组(5.15±1.03μg/ml vs 0.58±1.32μg/ml,P<0.05;19.46±3.28μg/ml vs 2.93±1.92μg/ml,P<0.05),而AT:A水平无显著差异(99.12±13.5%vs 100.8±10.6%,P>0.05)。结论先兆子痫患者存在PZ内含子FG79A基因多态性,但该基因多态性与疾病的发生关联不大,然而该疾病与PZ水平、D-D、FDP水平却有一定的相关性。
