Objective To investigate the effects of acute and chronic administration of the non-competitive NMDA receptor antagonists MK-801 on c-Fos protein expression in different brain regions of mice and an-tagonistic action ...Objective To investigate the effects of acute and chronic administration of the non-competitive NMDA receptor antagonists MK-801 on c-Fos protein expression in different brain regions of mice and an-tagonistic action of clozapine.Methods Immunohistochemistry was used to detect the expression of c-Fos protein.Results MK-801(0.6 mg·kg-1)acute administration produced a significant increase in the expression of c-Fos protein in the layers Ⅲ-Ⅳ of posterior cingulate and retrosplenial(PC/RS)cortex,which was consistent with the previous reports.Moreover,we presented a new finding that MK-801(0.6 mg·kg-1)chronic administration for 8 days produced a significant increase of c-Fos protein expression in the PC/RS cortex,prefrontal cortex(PFC)and hypothalamus of mice.Among that,c-Fos protein expression in the PC/RS cortex of mice was most significant.Compared acute administration with chronic administration,we found that MK-801 chronic administration significantly increased the expression of c-Fos protein in the PC/RS cortex,PFC and hypothalamus.Furthermore,pretreatment of mice with clozapine significantly decreased the expression of c-Fos protein induced by MK-801 acute and chronic administration.Conclusions Marked expression of c-Fos protein induced by MK-801 is associated with neurotransmitters' change noted in our previous studies,and c-Fos protein,the marker of neuronal activation,might play an important role in the chronic pathophysiological process of schizophrenic model induced by NMDA receptor antagonist.展开更多
目的:探讨便秘型肠易激综合征(C-IBS)大鼠模型对直肠球囊扩张的内脏敏感性改变和脊髓背角5-羟色胺(5-HT)、c-fos的分布和异常表达.方法:C-IBS大鼠模型组应用冰水ig方法建立(A组,n=10),和正常对照大鼠(B组,n=10).所有大鼠给予直肠内球囊...目的:探讨便秘型肠易激综合征(C-IBS)大鼠模型对直肠球囊扩张的内脏敏感性改变和脊髓背角5-羟色胺(5-HT)、c-fos的分布和异常表达.方法:C-IBS大鼠模型组应用冰水ig方法建立(A组,n=10),和正常对照大鼠(B组,n=10).所有大鼠给予直肠内球囊扩张,检测球囊扩张引起腹部收缩反射的最小容量阈值及球囊不同容量扩张时腹部收缩反射的次数.腰骶段脊髓背角5-HT和c-fos表达应用免疫组织化学染色及计算机图像分析系统半定量进行分析.结果:直肠内球囊扩张时,A组引起腹部收缩的最小容量阈值略高于B组,无明显统计学差并(0.59±0.09 vs 0.57±0.13,P>0.05).直肠球囊扩张体积1.0mL时A组腹部收缩反射次数低于B组(10.3±3.3 vs 18.3±5.5,P<0.05);体积1.5和2.0mL高容量扩张时两组无明显差异(P>0.05).A组腰骶段脊髓背角5-HT、c-fos阳性神经组织的面积均明显高于B组(5-HT面积:146.5±15.1 vs 109.3±18.5;5-HT OD:45826±2563.2 vs 29358±8965.5:c-fos面积:125.4±23.3 vs 88.7±23.2;c-fos OD:46258±4642 vs 33238±4587;均P<0.05).结论:C-IBS大鼠模型存在对直肠球囊扩张的内脏敏感性异常,脊髓背角5-HT、c-fos的异常表达可能参与C-IBS大鼠内脏敏感性异常的调节.展开更多
文摘Objective To investigate the effects of acute and chronic administration of the non-competitive NMDA receptor antagonists MK-801 on c-Fos protein expression in different brain regions of mice and an-tagonistic action of clozapine.Methods Immunohistochemistry was used to detect the expression of c-Fos protein.Results MK-801(0.6 mg·kg-1)acute administration produced a significant increase in the expression of c-Fos protein in the layers Ⅲ-Ⅳ of posterior cingulate and retrosplenial(PC/RS)cortex,which was consistent with the previous reports.Moreover,we presented a new finding that MK-801(0.6 mg·kg-1)chronic administration for 8 days produced a significant increase of c-Fos protein expression in the PC/RS cortex,prefrontal cortex(PFC)and hypothalamus of mice.Among that,c-Fos protein expression in the PC/RS cortex of mice was most significant.Compared acute administration with chronic administration,we found that MK-801 chronic administration significantly increased the expression of c-Fos protein in the PC/RS cortex,PFC and hypothalamus.Furthermore,pretreatment of mice with clozapine significantly decreased the expression of c-Fos protein induced by MK-801 acute and chronic administration.Conclusions Marked expression of c-Fos protein induced by MK-801 is associated with neurotransmitters' change noted in our previous studies,and c-Fos protein,the marker of neuronal activation,might play an important role in the chronic pathophysiological process of schizophrenic model induced by NMDA receptor antagonist.
文摘目的:探讨便秘型肠易激综合征(C-IBS)大鼠模型对直肠球囊扩张的内脏敏感性改变和脊髓背角5-羟色胺(5-HT)、c-fos的分布和异常表达.方法:C-IBS大鼠模型组应用冰水ig方法建立(A组,n=10),和正常对照大鼠(B组,n=10).所有大鼠给予直肠内球囊扩张,检测球囊扩张引起腹部收缩反射的最小容量阈值及球囊不同容量扩张时腹部收缩反射的次数.腰骶段脊髓背角5-HT和c-fos表达应用免疫组织化学染色及计算机图像分析系统半定量进行分析.结果:直肠内球囊扩张时,A组引起腹部收缩的最小容量阈值略高于B组,无明显统计学差并(0.59±0.09 vs 0.57±0.13,P>0.05).直肠球囊扩张体积1.0mL时A组腹部收缩反射次数低于B组(10.3±3.3 vs 18.3±5.5,P<0.05);体积1.5和2.0mL高容量扩张时两组无明显差异(P>0.05).A组腰骶段脊髓背角5-HT、c-fos阳性神经组织的面积均明显高于B组(5-HT面积:146.5±15.1 vs 109.3±18.5;5-HT OD:45826±2563.2 vs 29358±8965.5:c-fos面积:125.4±23.3 vs 88.7±23.2;c-fos OD:46258±4642 vs 33238±4587;均P<0.05).结论:C-IBS大鼠模型存在对直肠球囊扩张的内脏敏感性异常,脊髓背角5-HT、c-fos的异常表达可能参与C-IBS大鼠内脏敏感性异常的调节.
