期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
Effect of Triptolide on Expression of Oxidative Carbonyl Protein in Renal Cortex of Rats with Diabetic Nephropathy 被引量:15
1
作者 董兴刚 安增梅 +2 位作者 过源 周佳亮 秦涛 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2017年第1期25-29,共5页
The traditional Chinese medicine(Tripterygium wilfordii Hook.f., TWH) has been clinically used to treat primary and secondary renal diseases and proteinuria for nearly 40 years. However, there is a rare literature a... The traditional Chinese medicine(Tripterygium wilfordii Hook.f., TWH) has been clinically used to treat primary and secondary renal diseases and proteinuria for nearly 40 years. However, there is a rare literature about the effect of triptolide(the main active ingredient of TWH) on the expression of oxidative carbonyl protein(OCP) in diabetic nephropathy(DN). This study aimed to provide experimental evidence for triptolide treatment on DN through its effect on the expression of OCP, in order to investigate the effects of triptolide on the expression of OCP in rats with DN. Sixty SD rats were randomly divided into five groups: control group, high-dose triptolide(Th) group, low-dose triptolide(Tl) group, DN model group, and positive control(benazepril) group. The DN model was established using streptozotocin. Urinary protein excretion, fasting blood glucose(FBG), superoxide dismutase(SOD) in renal homogenate, malondialdehyde(MDA) in renal homogenate and renal nitrotyrosine by immunohistochemistry, and the expression of OCP by oxyblotimmune blotting were detected. In the DN model group, rat urinary protein excretion and renal MDA were significantly increased, while renal SOD significantly decreased and nitrotyrosine expression was obviously upregulated in the kidney. After triptolide treatment, 24-h urinary protein excretion(61.96±19.00 vs. 18.32±4.78 mg/day, P〈0.001), renal MDA(8.09±0.79 vs. 5.45±0.68 nmol/L, P〈0.001), and nitrotyrosine expression were decreased. Furthermore, renal OCP significantly decreased, while renal SOD(82.50±19.10 vs. 124.00±20.52 U/L, P〈0.001) was elevated. This study revealed that triptolide can down-regulate the expression of OCP in the renal cortex of DN rats. 展开更多
关键词 triptolide diabetic nephropathy nitrotyrosine oxidative carbonyl protein
下载PDF
Assessment of oxidative stress in chronic pancreatitis patients 被引量:4
2
作者 Mariette Verlaan Hennie MJ Roelofs +4 位作者 Annie van Schaik Geert JA Wanten Jan BMJ Jansen Wilbert HM Peters Joost PH Drenth 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第35期5705-5710,共6页
AIM: To assess the levels of antioxidant capacity and oxidative damage in blood of chronic pancreatitis (CP) patients in comparison with those in healthy control sub- jects, by using several different analytical techn... AIM: To assess the levels of antioxidant capacity and oxidative damage in blood of chronic pancreatitis (CP) patients in comparison with those in healthy control sub- jects, by using several different analytical techniques. METHODS: Thirty-five CP patients and 35 healthy con- trol subjects were investigated prospectively with re- spect to plasma levels of thiols, ferric reducing ability of plasma (FRAP, i.e. antioxidant capacity), levels of protein carbonyls and thiobarbituric acid reactive substances (TBARS). Additionally, we evaluated the production of reactive oxygen species (ROS) in whole blood. RESULTS: The antioxidative thiols including cysteine, cysteinylglycine and glutathione were significantly lower in CP patients. In addition, the non-enzymatic antioxi- dant capacity was significantly lower in CP patients, which correlated with the amount of oxidative protein (protein carbonyls) and the extent of lipid damage (TBARS), both were significantly higher in CP patients. The ROS production in whole blood after stimulation with phorbol 12-myritate 13-acetaat, demonstrated a strong tendency to produce more ROS in CP patients. CONCLUSION: Oxidative stress may contribute to the pathogenesis of chronic pancreatitis by decreasing anti- oxidant capacity and increasing oxidative damage in CP patients may be a rationale for intervention with antioxi- dant therapy. 展开更多
关键词 Chronic pancreatitis Oxidative stress THIOLS Ferric reducing ability of plasma protein carbonyls Thiobarbituric acid reactive substances Reactive oxygen species
下载PDF
Evaluation of oxidative and nitrosative stress in relapsing remitting multiple sclerosis
3
作者 Stefan Lukac Terezia Kalnovicova Jana Muchova 《Health》 2013年第11期1924-1928,共5页
Multiple sclerosis (MS) is a chronic autoimmune disorder affecting the central nervous system (CNS) through demyelination and neurodegeneration. Several lines of evidence support a role for oxidative and nitrative str... Multiple sclerosis (MS) is a chronic autoimmune disorder affecting the central nervous system (CNS) through demyelination and neurodegeneration. Several lines of evidence support a role for oxidative and nitrative stress (OS and NS) in pathogenesis of multiple sclerosis. The mechanism of influence of OS and NS on blood-brainbarrier (BBB) has critical importance for evaluating antioxidant therapies. As far as we know, markers of oxidative and nitrative stress in MS patients have been investigated independently for their relationship with the state of the blood-brain-barrier. Blood plasma samples of 58 patients with relapse-remitting MS (RRMS) with normal (Group A, n = 48, 36.2 ± 10.5 years) and damaged BBB (Group B, n = 10, 38.2 ± 11.2 years) and of 44 healthy controls (39.2 ± 14.9 years) were analyzed. TAS (total antioxidant plasma status), lipoperoxides, protein carbonyls, 3-nitrotyrosine and uric acid were evaluated in each group. Our results confirmed decreased TAS (Group A: 1.35 ± 0.55 mmol/l, P e level of lipoperoxidation positively correlated with the state of BBB (P of protein’s carbonyls (A: 0.48 ± 0.11 nmol/mg protein, P nmol/l, P ed damage to plasma proteins, what was confirmed by their positive mutual correlation (P The level of uric acid was physiological and correlated negatively with protein’s carbonyls (P 0.05) while there was no significant relationship with 3-nitotyrosine. The results suggest the role of this antioxidant in the protection of the proteins against OS what was confirmed by its positive correlation with TAS 展开更多
关键词 Relapsing Remiting Multiple Sclerosis Oxidative Stress Nitrative Stress BLOOD-BRAIN-BARRIER Total Antioxidant Status 3-NITROTYROSINE protein carbonyls Lipoperoxides Uric Acid
下载PDF
Star fruit extract and C-glycosylated flavonoid components have potential to prevent air pollutant-induced skin inflammation and premature aging
4
作者 Ping Wu Hiroyasu Iwahashi +5 位作者 Hai-Hui Xie Ying Wang Yan-Yang Zhou Akinori Kiso Yoshihito Kawashima Xiao-Yi Wei 《Natural Products and Bioprospecting》 2022年第1期158-167,共10页
Air pollution adversely affects skin,leading to skin inflammation and premature skin aging.Plant derived antioxidant compounds have been considered to be promising in discovery of effective agents for the protection o... Air pollution adversely affects skin,leading to skin inflammation and premature skin aging.Plant derived antioxidant compounds have been considered to be promising in discovery of effective agents for the protection of skin from the damage by air pollutants.Our previous studies demonstrated that Averrhoa carambola fruit(known as star fruit)is rich in flavonoid C-glycosides with unique structures and potent antioxidant activity.Thus,the star fruit extract(SFE)and main flavonoid C-glycoside components,carambolasides I,J,and P(1-3),carambolaflavone B(4),and isovitexin 2″-O-α-l-rhamnoside(5),were investigated for the activity against air pollutant stress in human epidermis.As a result,SFE and compounds 1-5 exhibited significant inhibitory activity against protein carbonylation in oxidative-stressed stratum corneum with the best activity being shown by compound 3.SFE and compounds 2-5 were also active against engine exhaust-induced protein carbonylation in stratum corneum.When further evaluated,SFE and com-pound 3 significantly inhibited gene expression of the key inflammation mediators IL-1αand COX-2 in PM-stressed keratinocytes.The results indicated that SFE and the flavonoid C-glycosides are potentially effective against air pollutant-induced skin inflammation and premature aging. 展开更多
关键词 Star fruit Carambolaside P Air pollution protein carbonylation Skin inflammation Premature skin aging
下载PDF
Mechanism of Novel Natural Products Maintaining Skin
5
作者 Chen Min Pang Ying Pan Sha 《China Detergent & Cosmetics》 2021年第4期56-60,共5页
The inducement of skin carbonylation was briefly introduced,then the mechanism of protein carbonylation from skin was discussed and adverse reactions to human skin were analyzed.Finally,according to the mechanism of p... The inducement of skin carbonylation was briefly introduced,then the mechanism of protein carbonylation from skin was discussed and adverse reactions to human skin were analyzed.Finally,according to the mechanism of protein carbonylation and the degradation mode,the proposed solutions were as follows:diglucosyl gallic acid,Hibiscus sabdariffa flower extract;Prunus cerasus flower extract can reduce the protein carbonylation level of skin by reducing t ROS and Phaeodactylum tricornutum extract can improve the activity of 20s enzyme and accelerating the hydrolysis level of carbonyl protein,to achieve the perfect repair of skin. 展开更多
关键词 protein carbonylation skin maintian diglucosyl gallic acid hibiscus sabdariffa flower extract
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部