In this letter,N-acryloyl-3-aminophenylboronic acid（AAPBA） was synthesized and then examined as a new functional monomer for protein imprinting.It was allowed to be copolymerized with acrylamide to produce hemoglobi...In this letter,N-acryloyl-3-aminophenylboronic acid（AAPBA） was synthesized and then examined as a new functional monomer for protein imprinting.It was allowed to be copolymerized with acrylamide to produce hemoglobin- or lysozyme-imprinted hydrogels.In template rebinding tests,the imprinted gels showed significant increase in the specific binding with the increase of the AAPBA amounts in the prepolymerization recipes.These results indicate that AAPBA may be a useful functional monomer for its moderate interactions with protein molecules.展开更多
Interpenetrated polymer networks of chitosan (CHI), polyacrylic acid (PAA) and polyacrylamide (PAM) were prepared by free radical polymerization. These hydrogels were either washed with double distilled water (CHI/PAA...Interpenetrated polymer networks of chitosan (CHI), polyacrylic acid (PAA) and polyacrylamide (PAM) were prepared by free radical polymerization. These hydrogels were either washed with double distilled water (CHI/PAA/PAM) A or hydrolyzed with 1M sodium hydroxide (NaOH), (CHI/PAA/PAM) S. Both types of hydrogels were characterized by infrared spectroscopy, microstructural techniques and compressive mechanical testing. Finally, hydrogels were loaded with bovine serum albumin (BSA) and release followed at different pHs. Infrared spectra analysis showed correspondence between hydrogels and monomer feed compositions. Hydrolyzed hydrogels, had increased water content and pH swelling dependence. Compression modulus of swelled hydrolyzed hydrogels decreased with increasing equilibrium water content. Higher BSA loadings were achieved on hydrolyzed hydrogels due to their high water content and porosity. Protein release from hydrogels was low (≤ 20% after 10 hours) at pH 1.2, but sustained release was observed at pH 6.8 and 7.4. The integrity of the protein released at 6.8 and 7.4 by hydrolyzed hydrogels was unaffected. The hydrogles showed no cytotoxic effects on human skin dermal fibroblasts as determined by MTT assay except for two compositions of (CHI/PAA/PAM) A samples, which after seven days presented a viability lower than 80% respect to the control.展开更多
Six different N-alkyl substituted acrylarnide nonionic hydrogels were prepared and their swelling characteristics were measured. Poly N-isopropyl acrylamide (PNIPA) and poly N-n-propyl-acrylamide (PNNPA) temperature s...Six different N-alkyl substituted acrylarnide nonionic hydrogels were prepared and their swelling characteristics were measured. Poly N-isopropyl acrylamide (PNIPA) and poly N-n-propyl-acrylamide (PNNPA) temperature sensitive hydrogels were chosen as the nonionic temperature sensitive hydrogels for concentration of very dilute aqueous protein solution. The separation properties of PNIPA and PNNPA hydr0gels with different network dimensions were studied and the modification of the hydrogels was surveyed in order to decrease their surface adsorption of protein molecules. The experimental results of the concentration of BSA (Bovin serum albumin) dilute aqueous solution by hydroxylpropyl methacrylate (HPMA) copolymerized PNIPA hydrogel were given. The value and the limitation of concentration of dilute aqueous protein solution by this method was evaluated.展开更多
目的:探究重组贻贝粘蛋白水凝胶敷料(Recombined mussel adhesive protein hydrogel dressing,Rmaphd)在点阵CO_(2)激光治疗面部痤疮萎缩性瘢痕术后创面修复中的应用效果。方法:选择2022年6月-2023年2月面部痤疮萎缩性瘢痕患者117例,分...目的:探究重组贻贝粘蛋白水凝胶敷料(Recombined mussel adhesive protein hydrogel dressing,Rmaphd)在点阵CO_(2)激光治疗面部痤疮萎缩性瘢痕术后创面修复中的应用效果。方法:选择2022年6月-2023年2月面部痤疮萎缩性瘢痕患者117例,分为Rmaphd组、重组人表皮生长因子(Recombinant human epidermal growth factor,rhEGF)组和对照组,每组39例,三组均给予点阵CO_(2)激光术治疗,术后分别给予Rmaphd、rhEGF及生理盐水处理,比较三组疗效、ECCA评分、症状持续时间以及生活质量评分。结果:Rmaphd组和rhEGF组总有效率分别为92.31%和94.87%,均高于对照组76.92%(P<0.05),术后ECCA评分低于对照组(P<0.05),术后疼痛、红斑、痂皮持续时间短于对照组(P<0.05),Acne-QoL各指标得分优于对照组(P<0.05);上述各临床Rmaphd组与rhEGF组差异无统计学意义(P>0.05)。结论:Rmaphd用于点阵CO_(2)激光治疗面部痤疮萎缩性瘢痕术后创面修复疗效显著,具备在临床上辅助激光治疗术后修复的应用潜力。展开更多
蛋白-多糖乳液凝胶作为活性物的包载体系,具有载量高、生物相容性高、稳定性好等优点。该文以螺旋藻分离蛋白(Spirulina protein isolate,SPI)和透明质酸(hyaluronic acid,HA)为乳化剂和凝胶基质,制备了不同油相体积分数的SPI乳液、SPI...蛋白-多糖乳液凝胶作为活性物的包载体系,具有载量高、生物相容性高、稳定性好等优点。该文以螺旋藻分离蛋白(Spirulina protein isolate,SPI)和透明质酸(hyaluronic acid,HA)为乳化剂和凝胶基质,制备了不同油相体积分数的SPI乳液、SPI-HA乳液和乳液凝胶,表征了乳液的微观结构和粒径,分析了不同油相体积分数对乳液凝胶形成的影响,并探究了乳液凝胶对β-胡萝卜素的高温保护效果。结果表明,SPI乳液随着HA的引入,乳液粒径明显降低;乳液凝胶结构则随着油相体积分数的增加越来越致密;当油相体积分数为60%时,乳液凝胶的屈服应力、储能模量以及表观黏度均高于SPI乳液和SPI-HA乳液;乳液凝胶对70℃,8 h下β-胡萝卜素的保留率高达(78.83±2.75)%。综上所述,SPI-HA乳液凝胶在食品领域中具有良好的应用前景。展开更多
Temperature-sensitive hydrogel—poly(N-isopropyl acrylamide) (PNIPA) was prepared and applied to protein refolding. PNIPA gel disks and gel particles were synthesized by the solution polymerization and inverse suspens...Temperature-sensitive hydrogel—poly(N-isopropyl acrylamide) (PNIPA) was prepared and applied to protein refolding. PNIPA gel disks and gel particles were synthesized by the solution polymerization and inverse suspension polymerization respectively. The swelling kinetics of the gels was also studied. With these prepared PNIPA gels, the model protein lysozyme was renatured. Within 24h, PNIPA gel disks improved the yield of lysozyme activity by 49.3% from 3375.2U·mg^-1 to 5038.8U·mg^-1. With the addition of faster response PNIPA gel beads, the total lysozyme activity recovery was about 68.98% in 3h, as compared with 42.03% by simple batch dilution. The novel refolding system with PNIPA enables efficient refolding especially at high protein concentrations. Discussion about the mechanism revealed that when PNIPA gels were added into the refolding buffer, the hydrophobic interactions between denatured proteins and polymer gels could prevent the aggregation of refolding intermediates, thus enhanced the protein renaturation.展开更多
豌豆分离蛋白(pea protein isolate,PPI)较差的凝胶性限制了其在食品中的应用。该文探究不同pH值(10、11、12)对转谷氨酰胺酶(transglutaminase,TG)诱导和葡萄糖酸-δ-内酯(glucono-δ-lactone,GDL)酸诱导豌豆分离蛋白凝胶(TG凝胶和GDL...豌豆分离蛋白(pea protein isolate,PPI)较差的凝胶性限制了其在食品中的应用。该文探究不同pH值(10、11、12)对转谷氨酰胺酶(transglutaminase,TG)诱导和葡萄糖酸-δ-内酯(glucono-δ-lactone,GDL)酸诱导豌豆分离蛋白凝胶(TG凝胶和GDL凝胶)特性和结构的影响。结果表明,pH值偏移可显著改善TG凝胶的性质,特别是pH12偏移改性后TG凝胶的持水性可达到最大值(92.13±2.40)%,硬度可达到最大值(413.82±4.90)g;游离巯基和非网络蛋白含量分别显著降低至(2.60±0.04)μmol/g和(6.00±0.40)%。TG添加量大于50 U/g时对凝胶的持水性和非网络蛋白含量没有显著影响。相反,pH值偏移对GDL凝胶的持水性和硬度无明显的改善作用。二级结构中α-螺旋和β-折叠含量的增加以及微观结构中孔径小、表面均匀致密的凝胶网络均表明pH值偏移可以显著改善TG凝胶的性质。展开更多
Neural electrodes,the core component of neural prostheses,are usually encapsulated in polydimethylsiloxane（PDMS）.However,PDMS can generate a tissue response after implantation.Based on the physicochemical properties...Neural electrodes,the core component of neural prostheses,are usually encapsulated in polydimethylsiloxane（PDMS）.However,PDMS can generate a tissue response after implantation.Based on the physicochemical properties and excellent biocompatibility of polyurethane（PU）and poly（vinyl alcohol）（PVA）when used as coating materials,we synthesized PU/PVA hydrogel coatings and coated the surface of PDMS using plasma treatment,and the cytocompatibility to rat pheochromocytoma（PC12）cells was assessed.Protein adsorption tests indicated that the amount of protein adsorption onto the PDMS substrate was reduced by 92%after coating with the hydrogel.Moreover,the PC12 cells on the PU/PVA-coated PDMS showed higher cell density and longer and more numerous neurites than those on the uncoated PDMS.These results indicate that the PU/PVA hydrogel is cytocompatible and a promising coating material for neural electrodes to improve their biocompatibility.展开更多
目的·探究水凝胶刚度对髓核细胞表型及其治疗大鼠椎间盘退变功能的影响。方法·构建不同浓度甲基丙烯酸酯明胶(methacrylate gelatin,GelMA)的水凝胶,使用流变分析和单轴压缩实验检测其刚度,扫描电子显微镜(scanning electron ...目的·探究水凝胶刚度对髓核细胞表型及其治疗大鼠椎间盘退变功能的影响。方法·构建不同浓度甲基丙烯酸酯明胶(methacrylate gelatin,GelMA)的水凝胶,使用流变分析和单轴压缩实验检测其刚度,扫描电子显微镜(scanning electron microscope,SEM)观察其微观结构和形态。将具有正常表型的髓核细胞接种于GelMA水凝胶表面,活/死细胞染色评价水凝胶的生物相容性,鬼笔环肽染色观察髓核细胞在不同刚度培养基质上的生长形态。免疫荧光染色追踪髓核细胞Yes相关蛋白(Yes-associated protein,YAP)的核定位,实时定量PCR检测髓核细胞相关基因[神经细胞黏附分子1(neural cell adhesion molecule 1,Ncam-1)、聚集蛋白聚糖(aggrecan,Acan)和Y染色体性别决定区(sex-determing region of Y chromosome,SRY)-盒转录因子9(SRY-box transcription factor 9,Sox9)]的表达水平。建立大鼠尾椎针刺椎间盘退变模型,获取不同培养基上的髓核细胞并分别注射到退变椎间盘中,4周后行磁共振成像检测,分析各实验组椎间盘含水量,组织学方法检测椎间盘结构和蛋白聚糖水平。结果·当GelMA预聚溶液浓度为4%和15%时,所得水凝胶的弹性模量分别为1 kPa和200 kPa。SEM显示水凝胶均呈疏松多孔结构,且水凝胶的孔隙率随其刚度增加而显著降低。体外实验显示2种刚度的GelMA水凝胶基质均有良好的生物相容性。相较于硬水凝胶基质(15%GelMA),软水凝胶基质(4%GelMA)上培养的髓核细胞伸长率更低、扩散面积更小,并表现出YAP在细胞质聚集的趋势。髓核细胞相关基因表达检测显示:软水凝胶基质组Sox9、Acan和Ncam-1的水平分别是对照组的23.7、6.6和12.7倍。体内实验显示,用不同刚度基质培养的髓核细胞治疗退变椎间盘,软水凝胶基质组的椎间盘含水量及结构完整度均高于硬水凝胶基质组。结论·相比于高刚度GelMA水凝胶,低刚度水凝胶基质能更好地维持髓核细胞的生长表型,并使其发挥更好的治疗椎间盘退变的功效。展开更多
基金supported by the National Natural Science Foundation of China(No.20574038)the Natural Science Foundation of Tianjin(No.09JCYBJC02900).
文摘In this letter,N-acryloyl-3-aminophenylboronic acid（AAPBA） was synthesized and then examined as a new functional monomer for protein imprinting.It was allowed to be copolymerized with acrylamide to produce hemoglobin- or lysozyme-imprinted hydrogels.In template rebinding tests,the imprinted gels showed significant increase in the specific binding with the increase of the AAPBA amounts in the prepolymerization recipes.These results indicate that AAPBA may be a useful functional monomer for its moderate interactions with protein molecules.
文摘Interpenetrated polymer networks of chitosan (CHI), polyacrylic acid (PAA) and polyacrylamide (PAM) were prepared by free radical polymerization. These hydrogels were either washed with double distilled water (CHI/PAA/PAM) A or hydrolyzed with 1M sodium hydroxide (NaOH), (CHI/PAA/PAM) S. Both types of hydrogels were characterized by infrared spectroscopy, microstructural techniques and compressive mechanical testing. Finally, hydrogels were loaded with bovine serum albumin (BSA) and release followed at different pHs. Infrared spectra analysis showed correspondence between hydrogels and monomer feed compositions. Hydrolyzed hydrogels, had increased water content and pH swelling dependence. Compression modulus of swelled hydrolyzed hydrogels decreased with increasing equilibrium water content. Higher BSA loadings were achieved on hydrolyzed hydrogels due to their high water content and porosity. Protein release from hydrogels was low (≤ 20% after 10 hours) at pH 1.2, but sustained release was observed at pH 6.8 and 7.4. The integrity of the protein released at 6.8 and 7.4 by hydrolyzed hydrogels was unaffected. The hydrogles showed no cytotoxic effects on human skin dermal fibroblasts as determined by MTT assay except for two compositions of (CHI/PAA/PAM) A samples, which after seven days presented a viability lower than 80% respect to the control.
基金This project sponsored by the National Natural Science Foundation of China.
文摘Six different N-alkyl substituted acrylarnide nonionic hydrogels were prepared and their swelling characteristics were measured. Poly N-isopropyl acrylamide (PNIPA) and poly N-n-propyl-acrylamide (PNNPA) temperature sensitive hydrogels were chosen as the nonionic temperature sensitive hydrogels for concentration of very dilute aqueous protein solution. The separation properties of PNIPA and PNNPA hydr0gels with different network dimensions were studied and the modification of the hydrogels was surveyed in order to decrease their surface adsorption of protein molecules. The experimental results of the concentration of BSA (Bovin serum albumin) dilute aqueous solution by hydroxylpropyl methacrylate (HPMA) copolymerized PNIPA hydrogel were given. The value and the limitation of concentration of dilute aqueous protein solution by this method was evaluated.
文摘目的:探究重组贻贝粘蛋白水凝胶敷料(Recombined mussel adhesive protein hydrogel dressing,Rmaphd)在点阵CO_(2)激光治疗面部痤疮萎缩性瘢痕术后创面修复中的应用效果。方法:选择2022年6月-2023年2月面部痤疮萎缩性瘢痕患者117例,分为Rmaphd组、重组人表皮生长因子(Recombinant human epidermal growth factor,rhEGF)组和对照组,每组39例,三组均给予点阵CO_(2)激光术治疗,术后分别给予Rmaphd、rhEGF及生理盐水处理,比较三组疗效、ECCA评分、症状持续时间以及生活质量评分。结果:Rmaphd组和rhEGF组总有效率分别为92.31%和94.87%,均高于对照组76.92%(P<0.05),术后ECCA评分低于对照组(P<0.05),术后疼痛、红斑、痂皮持续时间短于对照组(P<0.05),Acne-QoL各指标得分优于对照组(P<0.05);上述各临床Rmaphd组与rhEGF组差异无统计学意义(P>0.05)。结论:Rmaphd用于点阵CO_(2)激光治疗面部痤疮萎缩性瘢痕术后创面修复疗效显著,具备在临床上辅助激光治疗术后修复的应用潜力。
文摘蛋白-多糖乳液凝胶作为活性物的包载体系,具有载量高、生物相容性高、稳定性好等优点。该文以螺旋藻分离蛋白(Spirulina protein isolate,SPI)和透明质酸(hyaluronic acid,HA)为乳化剂和凝胶基质,制备了不同油相体积分数的SPI乳液、SPI-HA乳液和乳液凝胶,表征了乳液的微观结构和粒径,分析了不同油相体积分数对乳液凝胶形成的影响,并探究了乳液凝胶对β-胡萝卜素的高温保护效果。结果表明,SPI乳液随着HA的引入,乳液粒径明显降低;乳液凝胶结构则随着油相体积分数的增加越来越致密;当油相体积分数为60%时,乳液凝胶的屈服应力、储能模量以及表观黏度均高于SPI乳液和SPI-HA乳液;乳液凝胶对70℃,8 h下β-胡萝卜素的保留率高达(78.83±2.75)%。综上所述,SPI-HA乳液凝胶在食品领域中具有良好的应用前景。
基金the National Natural Science Foundation of China (No. 20276065).
文摘Temperature-sensitive hydrogel—poly(N-isopropyl acrylamide) (PNIPA) was prepared and applied to protein refolding. PNIPA gel disks and gel particles were synthesized by the solution polymerization and inverse suspension polymerization respectively. The swelling kinetics of the gels was also studied. With these prepared PNIPA gels, the model protein lysozyme was renatured. Within 24h, PNIPA gel disks improved the yield of lysozyme activity by 49.3% from 3375.2U·mg^-1 to 5038.8U·mg^-1. With the addition of faster response PNIPA gel beads, the total lysozyme activity recovery was about 68.98% in 3h, as compared with 42.03% by simple batch dilution. The novel refolding system with PNIPA enables efficient refolding especially at high protein concentrations. Discussion about the mechanism revealed that when PNIPA gels were added into the refolding buffer, the hydrophobic interactions between denatured proteins and polymer gels could prevent the aggregation of refolding intermediates, thus enhanced the protein renaturation.
文摘豌豆分离蛋白(pea protein isolate,PPI)较差的凝胶性限制了其在食品中的应用。该文探究不同pH值(10、11、12)对转谷氨酰胺酶(transglutaminase,TG)诱导和葡萄糖酸-δ-内酯(glucono-δ-lactone,GDL)酸诱导豌豆分离蛋白凝胶(TG凝胶和GDL凝胶)特性和结构的影响。结果表明,pH值偏移可显著改善TG凝胶的性质,特别是pH12偏移改性后TG凝胶的持水性可达到最大值(92.13±2.40)%,硬度可达到最大值(413.82±4.90)g;游离巯基和非网络蛋白含量分别显著降低至(2.60±0.04)μmol/g和(6.00±0.40)%。TG添加量大于50 U/g时对凝胶的持水性和非网络蛋白含量没有显著影响。相反,pH值偏移对GDL凝胶的持水性和硬度无明显的改善作用。二级结构中α-螺旋和β-折叠含量的增加以及微观结构中孔径小、表面均匀致密的凝胶网络均表明pH值偏移可以显著改善TG凝胶的性质。
基金supported by the National Natural Science Foundation of China,No.81170768grant from the Fundamental Research Project of Shanxi Province of China,No.2015021079
文摘Neural electrodes,the core component of neural prostheses,are usually encapsulated in polydimethylsiloxane（PDMS）.However,PDMS can generate a tissue response after implantation.Based on the physicochemical properties and excellent biocompatibility of polyurethane（PU）and poly（vinyl alcohol）（PVA）when used as coating materials,we synthesized PU/PVA hydrogel coatings and coated the surface of PDMS using plasma treatment,and the cytocompatibility to rat pheochromocytoma（PC12）cells was assessed.Protein adsorption tests indicated that the amount of protein adsorption onto the PDMS substrate was reduced by 92%after coating with the hydrogel.Moreover,the PC12 cells on the PU/PVA-coated PDMS showed higher cell density and longer and more numerous neurites than those on the uncoated PDMS.These results indicate that the PU/PVA hydrogel is cytocompatible and a promising coating material for neural electrodes to improve their biocompatibility.
文摘目的·探究水凝胶刚度对髓核细胞表型及其治疗大鼠椎间盘退变功能的影响。方法·构建不同浓度甲基丙烯酸酯明胶(methacrylate gelatin,GelMA)的水凝胶,使用流变分析和单轴压缩实验检测其刚度,扫描电子显微镜(scanning electron microscope,SEM)观察其微观结构和形态。将具有正常表型的髓核细胞接种于GelMA水凝胶表面,活/死细胞染色评价水凝胶的生物相容性,鬼笔环肽染色观察髓核细胞在不同刚度培养基质上的生长形态。免疫荧光染色追踪髓核细胞Yes相关蛋白(Yes-associated protein,YAP)的核定位,实时定量PCR检测髓核细胞相关基因[神经细胞黏附分子1(neural cell adhesion molecule 1,Ncam-1)、聚集蛋白聚糖(aggrecan,Acan)和Y染色体性别决定区(sex-determing region of Y chromosome,SRY)-盒转录因子9(SRY-box transcription factor 9,Sox9)]的表达水平。建立大鼠尾椎针刺椎间盘退变模型,获取不同培养基上的髓核细胞并分别注射到退变椎间盘中,4周后行磁共振成像检测,分析各实验组椎间盘含水量,组织学方法检测椎间盘结构和蛋白聚糖水平。结果·当GelMA预聚溶液浓度为4%和15%时,所得水凝胶的弹性模量分别为1 kPa和200 kPa。SEM显示水凝胶均呈疏松多孔结构,且水凝胶的孔隙率随其刚度增加而显著降低。体外实验显示2种刚度的GelMA水凝胶基质均有良好的生物相容性。相较于硬水凝胶基质(15%GelMA),软水凝胶基质(4%GelMA)上培养的髓核细胞伸长率更低、扩散面积更小,并表现出YAP在细胞质聚集的趋势。髓核细胞相关基因表达检测显示:软水凝胶基质组Sox9、Acan和Ncam-1的水平分别是对照组的23.7、6.6和12.7倍。体内实验显示,用不同刚度基质培养的髓核细胞治疗退变椎间盘,软水凝胶基质组的椎间盘含水量及结构完整度均高于硬水凝胶基质组。结论·相比于高刚度GelMA水凝胶,低刚度水凝胶基质能更好地维持髓核细胞的生长表型,并使其发挥更好的治疗椎间盘退变的功效。
