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A Core Genome Multilocus Sequence Typing Scheme for Proteus mirabilis
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作者 CHEN Sheng Lin KANG Yu Tong +2 位作者 LIANG Yi He QIU Xiao Tong LI Zhen Jun 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2023年第4期343-352,共10页
Objective A core genome multilocus sequence typing(cgMLST)scheme to genotype and identify potential risk clonal groups(CGs)in Proteus mirabilis.Methods In this work,we propose a publicly available cgMLST scheme for P.... Objective A core genome multilocus sequence typing(cgMLST)scheme to genotype and identify potential risk clonal groups(CGs)in Proteus mirabilis.Methods In this work,we propose a publicly available cgMLST scheme for P.mirabilis using chew BBACA.In total 72 complete P.mirabilis genomes,representing the diversity of this species,were used to set up a cgMLST scheme targeting 1,842 genes,635 unfinished(contig,chromosome,and scaffold)genomes were used for its validation.Results We identified a total of 205 CGs from 695 P.mirabilis strains with regional distribution characteristics.Of these,159 unique CGs were distributed in 16 countries.CG20 and CG3 carried large numbers of shared and unique antibiotic resistance genes.Nine virulence genes(papC,papD,papE,papF,papG,papH,papI,papJ,and papK)related to the P fimbrial operon that cause severe urinary tract infections were only found in CG20.These CGs require attention due to potential risks.Conclusion This research innovatively performs high-resolution molecular typing of P.mirabilis using whole-genome sequencing technology combined with a bioinformatics pipeline(chewBBACA).We found that the CGs of P.mirabilis showed regional distribution differences.We expect that our research will contribute to the establishment of cgMLST for P.mirabilis. 展开更多
关键词 proteus mirabilis CgMLST GENOTYpING Clonal evolution ChewBBACA
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Phenotypic and Antimicrobial Susceptibility Studies of Proteus mirabilis Isolates from Fresh Water Fishes in FCT, Abuja-Nigeria
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作者 Victor Bitrus Shammah Samuel Mailafia +4 位作者 James Agbo Ameh Casmir Ifeanyichukwu Cejetan Ifeanyi Adetola Opeyemi Adebari Ebenezer Odey Odey Reece Asoloko Sabo 《Advances in Microbiology》 CAS 2023年第5期249-265,共17页
Our study was carried out to determine the phenotypic characterization antimicrobial susceptibility of Proteus mirabilis from fish in FCT, Abuja using isolation, selective plating, preliminary observation, complete bi... Our study was carried out to determine the phenotypic characterization antimicrobial susceptibility of Proteus mirabilis from fish in FCT, Abuja using isolation, selective plating, preliminary observation, complete biochemical method and antimicrobial susceptibility testing. The biochemical tests conducted includes include Citrate Utilization test, Triple Sugar Iron test, Urea test, Methyl Red test, Indole test, and Voges Proskauer test. The isolates were confirmed by Microbact<sup>TM</sup>GNB24E identification kit (Oxiod, UK). A total of 400 fish samples were bought in the market from three area council of the FCT. The result of the study showed overall prevalence rate of (13) 3.25% of Proteus mirabilis isolates. Distribution based on Area councils showed that AMAC had higher prevalence rate of 4.81%, while Bwari had 2.99% and Gwagwalada with 2.57% prevalence. All isolates were subjected to antimicrobial susceptibility testing using the modified single disc diffusion method. From the antimicrobial susceptibility testing done it was discovered that Proteus mirabilis are resistant to Amoxyclav (100%), Erythromycin (92.3%), Tetracycline (92.3%) and Ceftriaxone (23.1%). However, the isolates were susceptible to Ofloxacin (100%), Netillin (92.3%), Levofloxacin (92.3%), Ceftazidime (76.9%), Co-trimoxazole (69.2%) and Gentamicin (61.5%). Since Proteus mirabilis sources of zoonotic diseases and can potentially be dangerous to humans and other animals, our research was able to isolate it from fresh water fish sold in the Federal Capital Territory. This makes public health awareness of the risks associated with Proteus mirabilis in Nigeria necessary. 展开更多
关键词 proteus mirabilis FISHES Isolation Microbactp>TMp>GNB24E BIOCHEMICAL Characterization pREVALENCE
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Antimicrobial Resistance and Genotype Analysis of Extended-Spectrum-β-Lactamase-Producing Proteus Mirabilis 被引量:2
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作者 Ying Huang Yuanhong Xu +1 位作者 Zhongxin Wang Xianghong Lin 《Open Journal of Clinical Diagnostics》 2014年第1期57-62,共6页
To analyse the genotypes of clinical isolates of Extended-Spectrum-β-Lactamase-Producing (ESBL-producing) Proteus mirabilis (P. mirabilis) and the mechanisms of antimicrobial resistance, to guide reasonable use of an... To analyse the genotypes of clinical isolates of Extended-Spectrum-β-Lactamase-Producing (ESBL-producing) Proteus mirabilis (P. mirabilis) and the mechanisms of antimicrobial resistance, to guide reasonable use of antibiotics and to avoid nosocomial outbreak infections by ESBL-producing P. mirabilis. 125 clinical isolates of P. mirabilis were collected from the Drug-Resistant Bacteria Surveillance Center of Anhui Province (from Jan 2009 to May 2010). Searching for the genotypes of ESBLs was perfomed by PCR amplification and DNA sequencing, and performed conjugation test simultaneously. Among ESBL-producing strains, CTX-M was the major genotype (3 CTX-M-13 and 1 CTX-M-3). TEM-1b spectrum β-lactamase was also prevalence in P. mirabilis. The diversity of β-lactamases in P. mirabilis and the emergency of multi-drug-resistance clinical strains will present serious threat to clinical therapy and even will lead to outbreak of nosocomial infections. Our study emphasizes the need for enhanced supervision of ESBL-producing P. mirabilis. Timely and reasonable drug-resistance data are indispensable to clinical therapy. 展开更多
关键词 GENOTYpE Extended-Spectrum-β-Lactamase ANTIMICROBIAL Resistance proteus mirabilis
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Extracellular Biosynthesis of AgNPs by the Bacterium <i>Proteus mirabilis</i>and Its Toxic Effect on Some Aspects of Animal Physiology 被引量:1
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作者 Mohammad S. Al-Harbi Bahig A. El-Deeb +1 位作者 Nasser Mostafa Sayed A. M. Amer 《Advances in Nanoparticles》 2014年第3期83-91,共9页
The development of a reliable green chemistry process for the biogenic synthesis of nanomaterials is an important aspect of current nanotechnology research. Silver nanoparticles (AgNPs) have been used as antimicrobial... The development of a reliable green chemistry process for the biogenic synthesis of nanomaterials is an important aspect of current nanotechnology research. Silver nanoparticles (AgNPs) have been used as antimicrobial and disinfectant agents. However, there is limited information about its toxicity. Therefore, this study focused on the biosynthesis of AgNPs by the bacterium Proteus mirabilis and on determining its preliminary toxic effect on some aspects of animal physiology. A green method for the synthesis of AgNPs using culture supernatant of Proteus mirabilis has been developed in this study and the synthesized AgNPs were characterized by several techniques. The AgNPs showed a maximum absorbance at 445 nm on ultraviolet-visible spectra. The presence of proteins was identified by Fourier transform-infrared spectroscopy. The reduction of Ag+ to elemental silver was characterized by X-ray spectroscopy analysis. The transmission electron micrograph revealed the formation of polydispersed nanoparticles of 5 - 45 nm. The AgNPs were evaluated for their toxic effect on pregnant female albino rat. The result showed that liver enzymes (AST and ALP) were decreased significantly in the group treated with AgNPs. Mean corpuscular hemoglobin concentration also showed significant increase. 展开更多
关键词 AGNpS p. mirabilis TEM X-Ray Liver ENZYMES Blood parameters
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Identification of the autotransporter Pet toxin in Proteus mirabilis strain isolated from patients with urinary tract infections 被引量:1
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作者 Luis Raúl Gutiérrez-Lucas Guillermo Mendoza-Hernández +3 位作者 Bertha González-Pedrajo Carlos Eslava-Campos Jaime Bustos-Martínez Teresita Sainz-Espunes 《Advances in Biological Chemistry》 2012年第3期283-290,共8页
Proteus mirabilis, a motile Gram-negative bacterium, represents a common cause of complicated urinary tract infections. Autotransporters are a family of secreted proteins from Gram-negative bacteria that direct their ... Proteus mirabilis, a motile Gram-negative bacterium, represents a common cause of complicated urinary tract infections. Autotransporters are a family of secreted proteins from Gram-negative bacteria that direct their own secretion across the outer membrane (type V autotransporter secretion mechanism). Serine protease autotransporters of Enterobacteriaceae (SPATEs) include adhesins, toxins, and proteases that can contribute to the virulence. Plasmid-encoded toxin (Pet) is the predominant protein in culture supernatants of enteroaggregative E. coli prototype strain 042 and has been extensively studied. Pet toxin is encoded on the 65-MDa adherence-related plasmid of EAEC 042 strain. In this work, Pet protein was found in the supernatant obtained from Proteus mirabilis RTX339 strain isolated from a psychiatric patient suffering complicated urinary tract infections (UTIs). The nucleotide sequence of pet gene was obtained using primers designed from E. coli 042 pet gene reported. The alignment of the sequence showed 100% identity with the pet gene reported. Is important to note that Proteus mirabilis RTX339 pet gene has chromosomal location. The chromosomal location of the gene was established since no plasmids were harbored by this strain. 展开更多
关键词 pET proteus mirabilis SpATEs
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Pathogenic infection and microbial composition of yellow catfish(Pelteobagrus fulvidraco)challenged by Aeromonas veronii and Proteus mirabilis
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作者 Wanying Zhai Qian Wang +2 位作者 Xinpeng Zhu Xiaoying Jia Liangbiao Chen 《Aquaculture and Fisheries》 CSCD 2023年第2期166-173,共8页
The catfishes are a group of economically important freshwater fish in China,which in recent years have suffered heavy losses as a result of bacterial outbreaks.In this study,we examined the diversity of the microbiom... The catfishes are a group of economically important freshwater fish in China,which in recent years have suffered heavy losses as a result of bacterial outbreaks.In this study,we examined the diversity of the microbiome of infected skin mucus of yellow catfish(Pelteobagrus fulvidraco)and channel catfish(Ictalurus punctatus)and analyzed the bacterial pathogens.We found several common pathogenic bacteria,such as Aeromonas spp.,Vibrio spp.,Moraxella spp.and Proteus spp.present in both fish species,but with significantly different bacterial community structures.We isolated and cultured Aeromonas veronii and Proteus mirabilis and validated their infectivity in zebrafish(Danio rerio)and yellow catfish.Intraperitoneal injection of either bacteria into zebrafish,but not immersion,caused 100%mortality and ovary fragmentation.Yellow catfish was more sensitive than zebrafish with 100%mortality in the immersion challenges with A.veronii or P.mirabilis,and with a higher abundance of A.veronii in the P.mirabilis-challenged group compared to control.To our knowledge,this is the first study on the pathogenicity of P.mirabilis in yellow catfish,and the results will help to develop effective strategies for the disease prevention and control in catfish farming. 展开更多
关键词 Aeromonas veronii proteus mirabilis Yellow catfish Channel catfish AQUACULTURE
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Stereoselective Synthesis and Immunological Evaluation of Common O-antigen of P. mirabilis OE and P. vulgaris TG103 被引量:2
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作者 Xiaopei Wu Jinsheng Lai +7 位作者 Kexin Yang Yunxia Xue Jinxi Liao Liming Wang Jian-song Sun Jing Hu Jian Yin Qingju Zhang 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2023年第5期535-543,共9页
Proteus species especially Proteus mirabilis and Proteus vulgaris are zoonotic pathogens which can cause public health disease.Owing to their antibiotic-resistance,developing vaccines against these pathogens is urgent... Proteus species especially Proteus mirabilis and Proteus vulgaris are zoonotic pathogens which can cause public health disease.Owing to their antibiotic-resistance,developing vaccines against these pathogens is urgently required.Herein,we describe the first synthesis of the common O-antigen of Proteus mirabilis OE and Proteus vulgaris TG 103. 展开更多
关键词 Stereoselective glycosylation proteus mirabilis OE proteus vulgaris TG 103 Common O-antigen Immunological evaluation Syntheticcarbohydrate-based vaccine Total synthesis
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Emergence of Klebsiella pneumoniae carbapenemase-producing Proteus mirabilis in Hangzhou, China 被引量:11
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作者 SHENG Zi-ke LI Jun-jie SHENG Guo-ping SHENG Ji-fang LI Lan-juan 《Chinese Medical Journal》 SCIE CAS CSCD 2010年第18期2568-2570,共3页
Background Carbapenems are used to treat severe infections caused by multi-drug-resistant organisms, however, the emergence of carbapenem-resistant bacterial isolates is becoming an increasing therapeutic challenge. S... Background Carbapenems are used to treat severe infections caused by multi-drug-resistant organisms, however, the emergence of carbapenem-resistant bacterial isolates is becoming an increasing therapeutic challenge. Since the first Klebsiella (K.) pneumoniae carbapenemase (KPC)-producing K. pneumoniae was reported in 2001, KPC-producing isolates have been found increasingly, specially in Enterobacteriaceae. The aim of this study was to characterize the mechanisms of a carbapenem-resistant Proteus (P.) mirabilis. Methods A carbapenem-resistant P. mirabilis isolate was recovered from pleural drainage fluid of a patient admitted to surgical intensive care unit. Antimicrobial susceptibility testing of the isolate was performed by disk diffusion according to Clinical and Laboratory Standards Institute guidelines, and subsequent minimal inhibitory concentrations were determined with the E-test. Amplification of the blaKPC gene generated a positive band and the PCR products were sequenced subsequently. The plasmid of the isolate was extracted and was successfully transformed into Escherichia (E.) coli DH5a. Results The P. mirabilis isolate was resistant to all detected antimicrobial agents except tigecycline. KPC-2 was confirmed by DNA sequence analysis. The transformant E. coil was resistant to carbapenems. Further study demonstrated that upstream and downstream regions of b/aKPC-2 were identical to that observed in K. pneumoniae submitted to GenBank from China in 2007. Conclusion Carbapenem resistance in the P. mirabilis isolate in this study is mainly due to production of KPC-2. 展开更多
关键词 ENTEROBACTERIACEAE proteus mirabilis carbapenem Klebsiella pneumoniae carbapenemase
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Multidrug-resistant Proteus mirabilis isolates carrying blaOXA-1 and blaNDM-1 from wildlife in China:increasing public health risk 被引量:5
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作者 Qian KANG Xue WANG +14 位作者 Jianan ZHAO Zhihui LIU Fang JI Han CHANG Jianchun YANG Shijia HU Ting JIA Xiaojia WANG Jiagui TANG Guoying DONG Guocheng HU Jing WANG Yanyu ZHANG Jianhua QIN Chengmin WANG 《Integrative Zoology》 SCIE CSCD 2021年第6期798-809,共12页
The emergence of multidrug resistance(MDR)in Proteus mirabilis clinical isolates is a growing public health concern and has serious implications for wildlife.What is the role of wildlife has been become one of the hot... The emergence of multidrug resistance(MDR)in Proteus mirabilis clinical isolates is a growing public health concern and has serious implications for wildlife.What is the role of wildlife has been become one of the hot issues in disseminating antimicrobial resistance.Here,54 P.mirabilis isolates from 12 different species were identified.Among them,25 isolates were determined to be MDR by profile of antimicrobial susceptibility;10 MDR P.mirabilis isolates were subjected to comparative genomic analysis by whole genome sequencing.Comprehensive analysis showed that chromosome of P.mirabilis isolates mainly carries multidrug-resistance complex elements harboring resistance to carbapenem genes blaOXA-1,blaNDM-1,and blaTEM-1.Class I integron is the insertion hotspot of IS26;it can be inserted into type I integron at different sites,thus forming a variety of multiple drug resistance decision sites.At the same time,Tn21,Tn7,and SXT/R391 mobile elements cause widespread spread of these drug resistance genes.In conclusion,P.mirabilis isolates from wildlife showed higher resistance to commonly used clinic drugs comparing to those from human.Therefore,wild animals carrying MDR clinical isolates should be paid attention to by the public health. 展开更多
关键词 blaNDM-1 blaOXA-1 MULTIDRUG-RESISTANT proteus mirabilis WILDLIFE
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Comparative outcomes of the pathogen in cultured Jones tubes used in lacrimal bypass surgery according to follow up periods
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作者 Bo Hyun Park Hui Kyung Kim +1 位作者 Yeon Ji Jo Jong Soo Lee 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第10期1623-1629,共7页
AIM:To evaluate the pathogens in cultured Jones tubes used in lacrimal bypass surgery according to the postoperative periods and to obtain data for the prevention of infection of functional lacrimal stent invention.ME... AIM:To evaluate the pathogens in cultured Jones tubes used in lacrimal bypass surgery according to the postoperative periods and to obtain data for the prevention of infection of functional lacrimal stent invention.METHODS:Totally 71 patients(81 eyes)who underwent the removal of Jones tubes were enrolled in study.All the removed Jones tubes were cultured for bacterial and fungal identification and tested for bacterial antibiotic sensitivity.The results were analyzed according to the duration of the inserted Jones tube after lacrimal bypass surgery.RESULTS:Of the 81 eyes,bacteria were isolated from 69 eyes(85.2%)and fungi from 6 eyes(7.4%).Among 69 eyes,40.6% showed Staphylococcus aureus(S.aureus),11.6% were Pseudomonas aeruginosa(P.aeruginosa).Gram-positive bacteria were isolated more than Gramnegative bacteria,but Gram-negative bacteria showed a higher incidence in the Jones tube implanted for over 10y(P=0.035).The antibiotic sensitivity test showed that 46.4% of S.aureus were resistant to oxacillin.In terms of antibiotics commonly used in ocular clinical practice,vancomycin was sensitive to S.aureus and Streptococcus pneumoniae(S.pneumoniae),amikacin responded to P.aeruginosa and Proteus mirabilis(P.mirabilis).Trimethoprim/sulfamethoxazole(TMP/SMX)was all sensitive to S.aureus,S.pneumoniae and P.mirabilis except P.aeruginosa.CONCLUSION:S.aureus is the most commonly found organism in the Jones tube after lacrimal bypass surgery,and 46.4% of them are methicillin-resistant S.aureus(MRSA),sensitive to vancomycin.Especially,P.mirabilis responded with amikacin is dominantly detected in the Jones tubes implanted for more than 10y. 展开更多
关键词 Jones tube lacrimal bypass surgery pseudomonas aeruginosa proteus mirabilis Staphylococcus aureus
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Pathological effects of vanillic acid on urinary tract in rats with infectious calculi
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作者 Shuai Guan Chuan Hao Cheng-Yong Li 《Journal of Hainan Medical University》 2020年第8期12-15,共4页
Objective:The pathological changes of urinary tract were observed after the treatment of infectious stone by vanillic acid.Methods:40 male Wistar rats were randomly divided into blank control group,infectious stone gr... Objective:The pathological changes of urinary tract were observed after the treatment of infectious stone by vanillic acid.Methods:40 male Wistar rats were randomly divided into blank control group,infectious stone group,vanillic acid group and acetohydroxamic acid group.Blank control group:4 male Wistar rats were fed without special treatment.Infectious Stone Group:12 male Wistar rats were implanted with 4 mm bacteria bearing foreign body in the front of the polyurethane vein indwelling needle infected with Proteus mirabilis into the bladder to induce the formation of infection stone.In the vanillic acid group,after the model were establishmented,12 male Wistar rats were treated with vanillic acid at 50mg/kg?d for 3 weeks.Acetohydroxamic acid group:after the model were establishmented,12 male Wistar rats were treated with acetohydroxamic acid at 12.5mg/kg?d for 3 weeks.The rats were killed 21 days later.The kidney,ureter and bladder were fixed in 10%paraformaldehyde solution for 24 hours.The specimens were embedded in paraffin,and the pathological analysis was carried out after HE and immunohistochemical staining.Results:In addition to the blank control group,the other three groups of rats had calculus formation in the bladder.No obvious pathological changes were found in the kidney,ureter and bladder in the blank control group,and inflammatory reactions were found in the other three groups:severe urinary tract inflammation was found in the infectious stone group,followed by acetylhydroxamic acid group.In the vanillic acid group,the pathological changes of urinary tract were the lightest,with a small amount of inflammatory cell infiltration and mucosal hyperplasia,but no serious pathological changes such as mucosal loss.The expression of Tamm-Horsfall Protein(THP)in the ascending branch of loop of Henle and the proximal part of distal tubule in vanillic acid group was not significant compared with that in the infectious stone group and acetohydroxamic acid group.Conclusion:Proteus mirabilis and its infective stones can lead to the damage of urinary epithelium.Vanillic acid can reduce the inflammatory lesions of urinary tissue. 展开更多
关键词 proteus mirabilis Vanillic acid Acetohydroxamic acid STONE INFECTION pATHOLOGY
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Investigation of the Potential Association between Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) and Antibiotic Resistance Pattern of Bacterial Strains Isolated from Medical Waste and Environmental Water
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作者 Md.Shahariar Alam Sajib Chakraborty +4 位作者 Taibur Rahman Md.Ismail Hosen Anik Paul A.K.M.Mahbub Hasan M.Anwar Hossain 《Open Journal of Medical Microbiology》 2018年第2期13-25,共13页
Enterobacteriaceae are important human pathogens that cause many food and waterborne illness. Rapid emergence of multi-drug resistant bacteria in Bangladesh has become a serious problem. Phage-host interaction is now ... Enterobacteriaceae are important human pathogens that cause many food and waterborne illness. Rapid emergence of multi-drug resistant bacteria in Bangladesh has become a serious problem. Phage-host interaction is now considered as the major driving force for the conversion of non-pathogenic bacteria to pathogenic ones. Evolution of highly pathogenic and antibiotic resistant bacteria largely depends upon the horizontal gene transfer by means of plasmid, megaplasmid and bacteriophages. Conversely, bacteria may acquire a novel defence mechanism called CRISPR (Clustered regularly interspersed short palindromic repeats) that can restrict horizontal transfer of plasmids and bacteriophages to limit the spread of antibiotic resistance genes among bacterial species. In this study, twenty bacterial strains were isolated from water of different medical waste and Buriganga river. Therefore, CRISPR locus was investigated following various biochemical and molecular analysis of those bacterial isolates. Identification of the bacterial isolates was conducted by Polymerase Chain Reaction (PCR) based assay of 16S rDNA extracted from those isolated strains. Results indicated that most strains were identified as Proteus mirabilis and Citrobacter freundii which mainly cause septicemia, and pneumonia in human. Thereafter, antibiogram of these strains was performed by using 11 different antibiotic discs where bacterial isolates from medical drainage system showed more resistant to antibiotics than the river water. In this study, CRISPR locus was also investigated within the genome of the isolated bacterial stains but unexpectedly, we did not find any CRISPR locus in their genome. In conclusion, we confirm that multi-drug resistant bacterial strains are devoid of CRISPR locus suggesting a possible negative association between CRISPR locus and antibiotic resistance. Further studies to pinpoint are required to elucidate the underlying mechanism of the association between CRISPR and antibiotic resistance in these isolated strains. 展开更多
关键词 ENTEROBACTERIACEAE Antibiotic Resistance proteus mirabilis CITROBACTER freundii CRISpR-Cas
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奇异变形杆菌毒力因子的研究进展 被引量:12
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作者 陈小敏 杨华 +3 位作者 桂国弘 温雪婷 戴宝玲 肖英平 《微生物学杂志》 CAS CSCD 2019年第1期109-114,共6页
奇异变形杆菌是革兰阴性细菌,在自然界中广泛存在,具有特殊的群集运动能力,与临床关系密切,可在膀胱和肾脏中形成结石,在尿道留置管外表面、内腔中形成结晶生物膜,是引起复杂尿路感染的主要病原体,主要的毒力因子包括菌毛、黏附素、尿... 奇异变形杆菌是革兰阴性细菌,在自然界中广泛存在,具有特殊的群集运动能力,与临床关系密切,可在膀胱和肾脏中形成结石,在尿道留置管外表面、内腔中形成结晶生物膜,是引起复杂尿路感染的主要病原体,主要的毒力因子包括菌毛、黏附素、尿素酶、溶血素、金属摄取和免疫逃避等。综述了近年来有关奇异变形杆菌毒力因子的研究,为相关研究提供参考。 展开更多
关键词 奇异变形杆菌(proteus mirabilis pM) 尿路感染 毒力因子
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棘胸蛙烂皮病奇异变形杆菌的分离、鉴定及对药物敏感性研究 被引量:35
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作者 王瑞君 熊筱娟 《淡水渔业》 CSCD 北大核心 2012年第4期31-34,共4页
从患烂皮病的棘胸蛙(Rana spinosa)中分离到一株致病菌,通过形态学、生理生化试验等方法鉴定该致病菌株为奇异变形杆菌(Proteus mirabilis)。同时,通过抑菌圈法研究了该菌株对11种抗生素、11种中草药、4种消毒剂的敏感性。结果显示:该... 从患烂皮病的棘胸蛙(Rana spinosa)中分离到一株致病菌,通过形态学、生理生化试验等方法鉴定该致病菌株为奇异变形杆菌(Proteus mirabilis)。同时,通过抑菌圈法研究了该菌株对11种抗生素、11种中草药、4种消毒剂的敏感性。结果显示:该菌株对头孢曲松、阿莫西林、链霉素、诺氟沙星、庆大霉素、卡那霉素、氯霉素等抗生素敏感,对四环素、红霉素不敏感;未观察到黄芪、茵陈对该菌的抑制作用,忍冬藤抑菌效果最好,其次依次为紫花地丁、金银花与甘草、鱼腥草、大青叶与柴胡、陈皮、黄连;致病菌株对消毒剂的敏感性依次为:高锰酸钾﹥三氯异氰脲酸﹥硫酸铜﹥氧化钙。 展开更多
关键词 棘胸蛙(Rana spinosa) 烂皮病 奇异变形杆菌(proteus mirabilis) 抗生素 中草药 消毒剂
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鼠奇异变形杆菌的鉴别诊断 被引量:8
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作者 高正琴 王春玲 +3 位作者 邢进 贺争鸣 高翔 邢瑞昌 《实验动物科学与管理》 2005年第4期46-49,共4页
目的对一株小鼠奇异变形杆菌进行了鉴定,为实验动物细菌检测、鉴别诊断提供参考依据。方法通过培养特性、菌落形态、染色和系列生化试验等检查,对疑似沙门氏菌的小鼠分离菌株进行初步鉴定,并采用血清凝集试验对小鼠分离菌株作进一步鉴... 目的对一株小鼠奇异变形杆菌进行了鉴定,为实验动物细菌检测、鉴别诊断提供参考依据。方法通过培养特性、菌落形态、染色和系列生化试验等检查,对疑似沙门氏菌的小鼠分离菌株进行初步鉴定,并采用血清凝集试验对小鼠分离菌株作进一步鉴定。结果通过表型生物学特性鉴定,并结合血清学诊断鉴别方法,确证该小鼠分离菌株为奇异变形杆菌。结论尽管检出的奇异变形杆菌不是SPF动物所必须排除的病原菌,但仍需指出:该菌为条件致病菌,对实验动物和研究人员均有潜在的危害,对科学实验也可能造成不利影响,故应引起高度重视。 展开更多
关键词 小鼠 奇异变形杆菌 鉴定
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大肠杆菌与变形杆菌引起母猪流产死亡报告 被引量:2
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作者 黄印尧 孔繁德 +3 位作者 周斌华 曹国进 庄耀宗 赖清金 《福建畜牧兽医》 1996年第1期2-4,共3页
本文在国内首次报道一起由埃希氏大肠杆菌和奇异变形杆菌联合引起母猪流产死亡病例及其疫情扑灭过程,同时证实这两菌混合感染对小百鼠的毒力大大高于单一菌,自家菌苗免疫是防制本病的最佳办法。
关键词 母猪 大肠杆菌 变形杆菌 防治 流产
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引起急性腹泻的两种变形杆菌的耐药性分析
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作者 陈日荣 李慧冰 《热带医学杂志》 CAS 2005年第5期677-678,共2页
目的了解引起急性腹泻的普通变形杆菌与奇异变形杆菌种类特征,耐药性与其菌种的关系。方法对2002年9月~2004年9月急性腹泻病人粪便中分离的奇异变形杆菌127株,普通变形杆菌30株进行鉴定。采用纸片扩散法进行药敏试验。结果普通变形杆... 目的了解引起急性腹泻的普通变形杆菌与奇异变形杆菌种类特征,耐药性与其菌种的关系。方法对2002年9月~2004年9月急性腹泻病人粪便中分离的奇异变形杆菌127株,普通变形杆菌30株进行鉴定。采用纸片扩散法进行药敏试验。结果普通变形杆菌对阿米卡星、头孢哌酮、头孢他定、头孢吡肟,亚胺培南耐药率在20%以下,可作为经验首选药物,对氨苄青霉素、头孢噻吩为100%耐药。但奇异变形杆菌对抗生素敏感性好,氨苄青霉素,头孢噻吩耐药率在23%以下。结论两种变形杆菌对抗生素的耐药性有较大区别,治疗时可根据菌种选择抗生素,同时,通过某些药敏试验结果还可以协助菌种鉴定。 展开更多
关键词 急性腹泻 变形杆菌 抗生素耐药性
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β-Cyclodextrin-modified AuBi metallic aerogels enable efficient peroxidase mimicking for colorimetric sensing of urease-positive pathogenic bacteria
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作者 Minghui Wang Peixian Wu +4 位作者 Sha Yang Gui-long Wu Na Li Xiaofeng Tan Qinglai Yang 《Nano Research》 SCIE EI CSCD 2023年第7期9663-9671,共9页
The detection of pathogenic bacteria with improved accessibility,reduced analysis time,and increased sensitivity is of great importance for diagnosing the infected disease.Nanozymes have attracted rising attention in ... The detection of pathogenic bacteria with improved accessibility,reduced analysis time,and increased sensitivity is of great importance for diagnosing the infected disease.Nanozymes have attracted rising attention in the bioassay field.Designing a model nanozyme needs the combined merit of sensible nanostructures and a large specific surface area to guarantee exceptional enzyme-mimic activity.Herein,aβ-cyclodextrin modified AuBi aerogel is prepared by a one-pot reduction strategy.The introduction ofβ-cyclodextrin(featured with a hydrophobic cavity and hydrophilic surface)enhances the catalytic activity of AuBi aerogels by engendering host-guest complex and improving dispersity/stability.Based on the specific urea hydrolysis,which could produce NH_(3)to raise pH by urease,the pH up-regulation would inhibit the peroxidase-mimicking performances ofβ-cyclodextrin/AuBi aerogels.Therefore,the sensitive colorimetric detection platform for urease activity could be constructed.Moreover,the sensing platform can detect straightforwardly urease-positive Proteus mirabilis in urine circumstances with a wide detection range and a low limit of detection(LOD)of 4 colony-forming unit(CFU)·mL^(-1).The reproducibility,stability,and specificity of this approach are verified to be satisfactory.Also,as an inhibitor of urease activity,the fluoride ion could be detected by the constructed sensing platform sensitively and specifically.Overall,this work provides a blueprint for designing an ideal nanozyme and paves a new roadway for detecting pathogenic bacteria. 展开更多
关键词 metallic aerogels surface modification urea hydrolysis urease activity fluoride ion proteus mirabilis bacteria detection
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貉源奇异变形杆菌分离鉴定及系统发育分析 被引量:5
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作者 张香斋 张召兴 +6 位作者 常超越 耿田田 李蕴玉 贾青辉 张艳英 李佩国 史秋梅 《黑龙江畜牧兽医(下半月)》 北大核心 2016年第12期193-195,共3页
为研究引起貉肺炎的病原,笔者无菌采集病貉肺脏、肝脏、心血等病料,分离出1株细菌并命名为H1,采用形态特征、培养特性、生化鉴定、动物致病性试验等方法进行了相关鉴定,初步确定该分离菌株为奇异变形杆菌(P.mirabilis)。结果表明:该... 为研究引起貉肺炎的病原,笔者无菌采集病貉肺脏、肝脏、心血等病料,分离出1株细菌并命名为H1,采用形态特征、培养特性、生化鉴定、动物致病性试验等方法进行了相关鉴定,初步确定该分离菌株为奇异变形杆菌(P.mirabilis)。结果表明:该分离菌株对小鼠具有较强致病作用;对H1菌株的16S rRNA序列在NCBI上利用BLAST软件与Gen Bank中公布的12株奇异变形杆菌参考株进行同源性对比分析,同源性为96.5%~99.9%,系统发育树分析发现,分离菌株H1与4株参考株亲缘关系较近,同源性均为99.9%,证明该分离菌株H1为奇异变形杆菌;该菌株对氟苯尼考、头孢曲松和阿奇霉素等高度敏感。 展开更多
关键词 奇异变形杆菌(p.mirabilis) 分离鉴定 系统发育分析 药敏试验
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奇异变形杆菌感染Caco-2细胞模型的建立
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作者 白芳 龚泽龙 +3 位作者 左乐 王淼 石晓路 袁建辉 《热带医学杂志》 CAS 2017年第3期328-332,F0002,共6页
目的建立奇异变形杆菌感染人克隆结肠腺癌细胞Caco-2的细胞模型,比较不同来源奇异变形杆菌感染Caco-2细胞的致病性特征。方法活细胞动态观察优化奇异变形杆菌感染Caco-2细胞的感染条件,采用台盼蓝细胞膜通透性检测、稀释平板计数、吉姆... 目的建立奇异变形杆菌感染人克隆结肠腺癌细胞Caco-2的细胞模型,比较不同来源奇异变形杆菌感染Caco-2细胞的致病性特征。方法活细胞动态观察优化奇异变形杆菌感染Caco-2细胞的感染条件,采用台盼蓝细胞膜通透性检测、稀释平板计数、吉姆萨(Giemsa)染色及透射电子显微镜观察不同来源奇异变形杆菌感染Caco-2细胞后细胞形态学及胞内感染情况的变化,评价该模型中不同来源奇异变形杆菌侵入细胞的特点和病理损伤规律。结果不同来源奇异变形杆菌感染Caco-2细胞的最佳感染复数(MOI)均为100∶1,感染时间均为2 h。致腹泻奇异变形杆菌C02011感染Caco-2细胞的细胞膜通透性改变,黏附率为(0.647±0.038)%,侵袭率为(0.261±0.005)%。健康人群体检株奇异变形杆菌B02005的细胞膜通透性出现轻微受损,黏附率为(0.975±0.030)%,侵袭率为(0.134±0.003)%。尿道分离奇异变形杆菌标准株ATCC29906的黏附率为(0.303±0.027)%,侵袭率为(0.104±0.003)%,并未发现Caco-2的细胞膜发生变化。吉姆萨染色和透射电子显微镜观察与上述方法结果一致,说明了不同来源奇异变形杆菌感染Caco-2细胞的致病性不同(P<0.05);同时,透射电子显微镜实验也验证了细胞模型的成功建立。结论成功构建奇异变形杆菌感染Caco-2的细胞模型,并分析了不同来源奇异变形杆菌的致病性特征。 展开更多
关键词 奇异变形杆菌 CACO-2细胞模型 透射电子显微镜
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