During oncogenesis,the hyper-activation of proto-oncogenes and defection of tumor suppressor genes（Zhao et al.,2012,2016a）can regulate cell proliferation,differentiation,apoptosis,and cell-to-cell communication（Bal...During oncogenesis,the hyper-activation of proto-oncogenes and defection of tumor suppressor genes（Zhao et al.,2012,2016a）can regulate cell proliferation,differentiation,apoptosis,and cell-to-cell communication（Balmain et al.,2003;Haber and Settleman,2007）.Recent evidence has shown that non-coding RNAs, such as microRNAs （miRNAs） （Chen, 2005）, and long non- coding RNAs （lncRNAs）, can also act as oncogenes to initiate and promote cancer progression.展开更多
Objective To explore the effects of liposome C erbB 2 antisense phosphorothioate oligodeoxynucleotides (S ODNs) on C erbB 2 proto oncogene expression and cell proliferation in human ovarian cancer cells Metho...Objective To explore the effects of liposome C erbB 2 antisense phosphorothioate oligodeoxynucleotides (S ODNs) on C erbB 2 proto oncogene expression and cell proliferation in human ovarian cancer cells Methods The effects of liposome C erbB 2 S ODNs on C erbB 2 protein expression, cell cycle and cell proliferation in human ovarian cancer cells were studied by means of flow cytometry and 3H thymidine incorporation Results Liposome C erbB 2 S ODNs can specifically reduce C erbB 2 protein expression in human ovarian cancer cells, accompanied by a 30% inhibition of cell proliferation The effectiveness of liposome C erbB 2 S ODNs on the expression of C erbB 2 was about 40 times higher than that of C erbB 2 S ODNs Conclusions The data suggest that antisense therapy might be a useful method of gene therapy in ovarian cancer The effectiveness of C erbB 2 S ODNs could be greatly increased by adsorption of S ODNs by liposomes展开更多
文摘During oncogenesis,the hyper-activation of proto-oncogenes and defection of tumor suppressor genes（Zhao et al.,2012,2016a）can regulate cell proliferation,differentiation,apoptosis,and cell-to-cell communication（Balmain et al.,2003;Haber and Settleman,2007）.Recent evidence has shown that non-coding RNAs, such as microRNAs （miRNAs） （Chen, 2005）, and long non- coding RNAs （lncRNAs）, can also act as oncogenes to initiate and promote cancer progression.
基金ThisworkwassupportedbytheNationalNaturalScienceFoundationof China (No 39470 72 4)
文摘Objective To explore the effects of liposome C erbB 2 antisense phosphorothioate oligodeoxynucleotides (S ODNs) on C erbB 2 proto oncogene expression and cell proliferation in human ovarian cancer cells Methods The effects of liposome C erbB 2 S ODNs on C erbB 2 protein expression, cell cycle and cell proliferation in human ovarian cancer cells were studied by means of flow cytometry and 3H thymidine incorporation Results Liposome C erbB 2 S ODNs can specifically reduce C erbB 2 protein expression in human ovarian cancer cells, accompanied by a 30% inhibition of cell proliferation The effectiveness of liposome C erbB 2 S ODNs on the expression of C erbB 2 was about 40 times higher than that of C erbB 2 S ODNs Conclusions The data suggest that antisense therapy might be a useful method of gene therapy in ovarian cancer The effectiveness of C erbB 2 S ODNs could be greatly increased by adsorption of S ODNs by liposomes
