[Objectives] This study was conducted to optimize the extraction process of protocatechuic acid from Emilia sonchifolia DC. [Methods] The optimal extraction conditions were determined by single factor,response surface...[Objectives] This study was conducted to optimize the extraction process of protocatechuic acid from Emilia sonchifolia DC. [Methods] The optimal extraction conditions were determined by single factor,response surface analysis and variance analysis,and the content of protocatechuic acid was determined by HPLC. [Results] The protocatechuic acid standard curve equation was: y = 1 435 x + 8 403,R^2= 0. 999 8,indicating a good linear relationship. The optimal extraction conditions were as follows: a temperature at 80 ℃,an extraction time of 1 h,a material-to-liquid ratio at 1:10 and an ultrasonic power of 600 W,and the content of protocatechuic acid extracted was 1. 93 mg/g. The method showed a RSD of 0. 41%,less than 2%,and the detection limit was 0. 0000047261 g/ml.The experimental sample X1 was the low-level 0. 1 mg/ml standard solution,which showed recovery of protocatechuic acid between 100.8% and 105.2%,with a RSD of 0. 013%;and the sample X2 was the high-level 1. 0 mg/ml standard solution,which exhibited recovery between 100. 6% and 102. 2%,with a RSD of 0.076%. Thus,the recovery was high,and the requirements of the performance index were met. [Conclusions] The detection method is stable and reliable and can produce satisfactory results.展开更多
AIM:To evaluate the contribution of alcohol dehydrogenase-1B(ADH1B)and aldehyde dehydrogenase-2 (ALDH2)polymorphisms to the risk of esophageal cancer.METHODS:Nineteen articles were included by searching MEDLINE,EMBASE...AIM:To evaluate the contribution of alcohol dehydrogenase-1B(ADH1B)and aldehyde dehydrogenase-2 (ALDH2)polymorphisms to the risk of esophageal cancer.METHODS:Nineteen articles were included by searching MEDLINE,EMBASE and the Chinese Biomedical Database,13 on ADH1B and 18 on ALDH2.We performed a meta-analysis of case-control studies including 13 studies on ADH1B(cases/controls:2390/7100)and 18 studies on ALDH2(2631/6030).RESULTS:The crude odds ratio[OR(95%confidence interval)]was 2.91(2.04-4.14)for ADH1B*1/*1(vs ADH1B*2/*2)and 1.32(1.17-1.49)for ADH1B*1/*2.The crude OR for ALDH2*1/*2(vs ALDH2*1/*1)was 2.52(1.76-3.61).ADH1B*1/*1 increased the risk of esophageal cancer among never/rare[1.56(0.93-2.61)],moderate[2.71(1.37-5.35)],and heavy drinkers[3.22 (2.27-4.57)].ADH1B*1/*2 was associated with a modest risk among moderate drinkers[1.43(1.09-1.87)].ALDH2*1/*2 increased the risk among never/rare[1.28 (0.91-1.80)],moderate[3.12(1.95-5.01)],and heavy [7.12(4.67-10.86)]drinkers,and among ex-drinkers [5.64(1.57-20.25)].ALDH2*2/*2 increased the risk among drinkers[4.42(1.72-11.36)].ADH1B*1/*1 plus ALDH2*1/*2 was associated with the highest risk for heavy drinkers[12.45(2.9-53.46)].The results of the meta-regression analysis showed that the effects of ADH1B*1/*1 and ALDH2*1/*2 increased with the level of alcohol consumption.ALDH2*1/*2 was associated with a high risk among Taiwan Chinese and Japanese drinkers,as opposed to a moderate risk among drinkers in high-incidence regions of China's Mainland.ADH1B*1/*1 in heavy drinkers and ALDH2*1/*2 in moderate-toheavy drinkers was associated with similarly high risk among both men and women.CONCLUSION:ADH1B/ALDH2 genotypes affect the risk of esophageal cancer,and the risk is modified by alcohol consumption,ethnicity,and gender.展开更多
Rescuing cells from stress damage emerges a potential therapeutic strategy to combat myocardial infarction.Protocatechuic aldehyde(PCA)is a major phenolic acid in Chinese herb Danshen(Salvia miltiorrhiza root).This st...Rescuing cells from stress damage emerges a potential therapeutic strategy to combat myocardial infarction.Protocatechuic aldehyde(PCA)is a major phenolic acid in Chinese herb Danshen(Salvia miltiorrhiza root).This study investigated whether PCA regulated nuclear pyruvate kinase isoform M2(PKM2)function to protect cardiomyocytes.In rats subjected to isoprenaline,PCA attenuated heart injury and protected cardiomyocytes from apoptosis.Through DARTS and CETSA assays,we identified that PCA bound and promoted PKM2 nuclear translocation in cardiomyocytes exposed to oxygen/glucose deprivation(OGD).In the nucleus,PCA increased the binding of PKM2 to β-Catenin via preserving PKM2 acetylation,and the complex,in cooperation with T-cell factor 4(TCF4),was required for transcriptional induction of genes encoding anti-apoptotic proteins,contributing to rescuing cardiomyocyte survival.In addition,PCA ameliorated mitochondrial dysfunction and prevented mitochondrial apoptosis dependent on PKM2.Consistently,PCA increased the binding of PKM2 to β-Catenin,improved heart contractive function,normalized heart structure and attenuated oxidative damage in mice subjected to artery ligation,but the protective effects were lost in Pkm2-deficient heart.Together,we showed that PCA regulated nuclear PKM2 function to rescue cardiomyocyte survival via β-Catenin/TCF4 signaling cascade,suggesting the potential of pharmacological intervention of PKM2 shuttle to protect the heart.展开更多
Background Alcohol dehydrogenase （ADH） and aldehyde dehydrogenase 2 （ALDH2） are the key en- zymes for alcohol metabolism. Several genetic studies have investigated the association between ADH and ALDH2 genetic pol...Background Alcohol dehydrogenase （ADH） and aldehyde dehydrogenase 2 （ALDH2） are the key en- zymes for alcohol metabolism. Several genetic studies have investigated the association between ADH and ALDH2 genetic polymorphisms and serum lipid profile （SLP）, however, the results were inconsistent. Methods Fourteen articles involving 27,917 participants were included in this meta-analysis. Weighted mean difference （WMD） and 95% confidence intervals （CIs） were presented using random effects model. Publication bias was evaluated by funnel plot and Begg＇s test. In addition, to further explore the heterogeneity, subgroup analysis were performed. Results Overall, there was no association between ADH genetic polymorphisms and SLP with no regard for drinking status. However, compared with ALDH2 wild homozygous genotype, ALDH2 mutant genotypes were associated with significant decrease in serum high density lipoprotein cholesterol （HDL- C） （WMD -1.80 mg/dL, 95% CI -1.88 to -1.72, P 〈 0.001） and total cholesterol （TC） levels （WMD -1.]0 mg/dL, 95% CI -1.59 to -0.62, P 〈 0.001）, and significant increase in serum low density lipoprotein choles- terol （LDL-C） level （WMD 0.30 mg/dL, 95% CI 0.18 to 0.43, P 〈 0.001）. Although there was no significant difference in serum triglyceride level in the overall population, subgroup analysis revealed that compared with ALDH2 ＊ 1 wild homozygote, ALDH2 ＊ 2 allele displayed a significant difference in serum triglyceride level between the female and male （female： WMD 1.69 mg/dl, 95% CI 1.08 to 2.30, P 〈 0.001; male： WMD -6.42 mg/dL, 95% CI -12.15 to -0.68, P = 0.028）. Conclusion ADH genetic polymorphism has no association with SLP, regardless of sex category and drinking status. ALDH2 genetic polymorphism has slight association with HDL-C, LDL-C and TC levels and sex-specific association with serum triglyceride level. Whether or not the association between ADH2 genetic polymorphisms and SLP is resulted from alcohol con-sumption needs further investigation.展开更多
An aldehyde-reactive probe based on 2-amino benzamidoxime(ABAO)framework was introduced,which can selectively label aldehydes in DNA through intramolecular ring closure under mild aqueous solutions.We screened ABAO de...An aldehyde-reactive probe based on 2-amino benzamidoxime(ABAO)framework was introduced,which can selectively label aldehydes in DNA through intramolecular ring closure under mild aqueous solutions.We screened ABAO derivatives that can undergo a cyclization with the formylated nucleobases to generate a fluorescence nucleoside,and of these derivatives 5–methoxy-ABAO(PMA)emerged as the optimal choice.PMA can sensitively and selectively react with 5f U,5f C and AP to form fluorogenic dihydroquinazoline derivatives,which also can quantify DNA damages induced byγ-irradiation.PMA-initiated labeling strategy provides great convenience for qualitative and quantitative detection of aldehydes in DNA.展开更多
基金Supported by Basic and Applied Basic Research Plan of Hainan Province in2019 (2019RC245)Natural Science Foundation of Hainan Province in 2017(217157)。
文摘[Objectives] This study was conducted to optimize the extraction process of protocatechuic acid from Emilia sonchifolia DC. [Methods] The optimal extraction conditions were determined by single factor,response surface analysis and variance analysis,and the content of protocatechuic acid was determined by HPLC. [Results] The protocatechuic acid standard curve equation was: y = 1 435 x + 8 403,R^2= 0. 999 8,indicating a good linear relationship. The optimal extraction conditions were as follows: a temperature at 80 ℃,an extraction time of 1 h,a material-to-liquid ratio at 1:10 and an ultrasonic power of 600 W,and the content of protocatechuic acid extracted was 1. 93 mg/g. The method showed a RSD of 0. 41%,less than 2%,and the detection limit was 0. 0000047261 g/ml.The experimental sample X1 was the low-level 0. 1 mg/ml standard solution,which showed recovery of protocatechuic acid between 100.8% and 105.2%,with a RSD of 0. 013%;and the sample X2 was the high-level 1. 0 mg/ml standard solution,which exhibited recovery between 100. 6% and 102. 2%,with a RSD of 0.076%. Thus,the recovery was high,and the requirements of the performance index were met. [Conclusions] The detection method is stable and reliable and can produce satisfactory results.
文摘AIM:To evaluate the contribution of alcohol dehydrogenase-1B(ADH1B)and aldehyde dehydrogenase-2 (ALDH2)polymorphisms to the risk of esophageal cancer.METHODS:Nineteen articles were included by searching MEDLINE,EMBASE and the Chinese Biomedical Database,13 on ADH1B and 18 on ALDH2.We performed a meta-analysis of case-control studies including 13 studies on ADH1B(cases/controls:2390/7100)and 18 studies on ALDH2(2631/6030).RESULTS:The crude odds ratio[OR(95%confidence interval)]was 2.91(2.04-4.14)for ADH1B*1/*1(vs ADH1B*2/*2)and 1.32(1.17-1.49)for ADH1B*1/*2.The crude OR for ALDH2*1/*2(vs ALDH2*1/*1)was 2.52(1.76-3.61).ADH1B*1/*1 increased the risk of esophageal cancer among never/rare[1.56(0.93-2.61)],moderate[2.71(1.37-5.35)],and heavy drinkers[3.22 (2.27-4.57)].ADH1B*1/*2 was associated with a modest risk among moderate drinkers[1.43(1.09-1.87)].ALDH2*1/*2 increased the risk among never/rare[1.28 (0.91-1.80)],moderate[3.12(1.95-5.01)],and heavy [7.12(4.67-10.86)]drinkers,and among ex-drinkers [5.64(1.57-20.25)].ALDH2*2/*2 increased the risk among drinkers[4.42(1.72-11.36)].ADH1B*1/*1 plus ALDH2*1/*2 was associated with the highest risk for heavy drinkers[12.45(2.9-53.46)].The results of the meta-regression analysis showed that the effects of ADH1B*1/*1 and ALDH2*1/*2 increased with the level of alcohol consumption.ALDH2*1/*2 was associated with a high risk among Taiwan Chinese and Japanese drinkers,as opposed to a moderate risk among drinkers in high-incidence regions of China's Mainland.ADH1B*1/*1 in heavy drinkers and ALDH2*1/*2 in moderate-toheavy drinkers was associated with similarly high risk among both men and women.CONCLUSION:ADH1B/ALDH2 genotypes affect the risk of esophageal cancer,and the risk is modified by alcohol consumption,ethnicity,and gender.
基金supported by the National Key R&D Program of China(2019YFC1711000)the National Natural Science Foundation of China(81421005 and 81722048)“Double First-Class”University project(CPU2018GF04 and CPU2018GY09,China)。
文摘Rescuing cells from stress damage emerges a potential therapeutic strategy to combat myocardial infarction.Protocatechuic aldehyde(PCA)is a major phenolic acid in Chinese herb Danshen(Salvia miltiorrhiza root).This study investigated whether PCA regulated nuclear pyruvate kinase isoform M2(PKM2)function to protect cardiomyocytes.In rats subjected to isoprenaline,PCA attenuated heart injury and protected cardiomyocytes from apoptosis.Through DARTS and CETSA assays,we identified that PCA bound and promoted PKM2 nuclear translocation in cardiomyocytes exposed to oxygen/glucose deprivation(OGD).In the nucleus,PCA increased the binding of PKM2 to β-Catenin via preserving PKM2 acetylation,and the complex,in cooperation with T-cell factor 4(TCF4),was required for transcriptional induction of genes encoding anti-apoptotic proteins,contributing to rescuing cardiomyocyte survival.In addition,PCA ameliorated mitochondrial dysfunction and prevented mitochondrial apoptosis dependent on PKM2.Consistently,PCA increased the binding of PKM2 to β-Catenin,improved heart contractive function,normalized heart structure and attenuated oxidative damage in mice subjected to artery ligation,but the protective effects were lost in Pkm2-deficient heart.Together,we showed that PCA regulated nuclear PKM2 function to rescue cardiomyocyte survival via β-Catenin/TCF4 signaling cascade,suggesting the potential of pharmacological intervention of PKM2 shuttle to protect the heart.
基金supported by National Natural Science Foundation of China(No.81301676)the PhD Start-up Program of Guangdong Province Natural Science Fund(No.S2012040008047)
文摘Background Alcohol dehydrogenase （ADH） and aldehyde dehydrogenase 2 （ALDH2） are the key en- zymes for alcohol metabolism. Several genetic studies have investigated the association between ADH and ALDH2 genetic polymorphisms and serum lipid profile （SLP）, however, the results were inconsistent. Methods Fourteen articles involving 27,917 participants were included in this meta-analysis. Weighted mean difference （WMD） and 95% confidence intervals （CIs） were presented using random effects model. Publication bias was evaluated by funnel plot and Begg＇s test. In addition, to further explore the heterogeneity, subgroup analysis were performed. Results Overall, there was no association between ADH genetic polymorphisms and SLP with no regard for drinking status. However, compared with ALDH2 wild homozygous genotype, ALDH2 mutant genotypes were associated with significant decrease in serum high density lipoprotein cholesterol （HDL- C） （WMD -1.80 mg/dL, 95% CI -1.88 to -1.72, P 〈 0.001） and total cholesterol （TC） levels （WMD -1.]0 mg/dL, 95% CI -1.59 to -0.62, P 〈 0.001）, and significant increase in serum low density lipoprotein choles- terol （LDL-C） level （WMD 0.30 mg/dL, 95% CI 0.18 to 0.43, P 〈 0.001）. Although there was no significant difference in serum triglyceride level in the overall population, subgroup analysis revealed that compared with ALDH2 ＊ 1 wild homozygote, ALDH2 ＊ 2 allele displayed a significant difference in serum triglyceride level between the female and male （female： WMD 1.69 mg/dl, 95% CI 1.08 to 2.30, P 〈 0.001; male： WMD -6.42 mg/dL, 95% CI -12.15 to -0.68, P = 0.028）. Conclusion ADH genetic polymorphism has no association with SLP, regardless of sex category and drinking status. ALDH2 genetic polymorphism has slight association with HDL-C, LDL-C and TC levels and sex-specific association with serum triglyceride level. Whether or not the association between ADH2 genetic polymorphisms and SLP is resulted from alcohol con-sumption needs further investigation.
基金financially supported by National Natural Science Foundation of China(Nos.22077088 and 21877082)Foundation from Science and Technology Department of Sichuan Province(Nos.2020JDJQ0017,2021YFH0132)。
文摘An aldehyde-reactive probe based on 2-amino benzamidoxime(ABAO)framework was introduced,which can selectively label aldehydes in DNA through intramolecular ring closure under mild aqueous solutions.We screened ABAO derivatives that can undergo a cyclization with the formylated nucleobases to generate a fluorescence nucleoside,and of these derivatives 5–methoxy-ABAO(PMA)emerged as the optimal choice.PMA can sensitively and selectively react with 5f U,5f C and AP to form fluorogenic dihydroquinazoline derivatives,which also can quantify DNA damages induced byγ-irradiation.PMA-initiated labeling strategy provides great convenience for qualitative and quantitative detection of aldehydes in DNA.
