Identification of Hybrids between Prunus salicina and Prunus armeniaca by RAPD and SSR

[ Objective] This study aimed to investigate the genetic variation between hybrids and their parents, and to find a rapid identification method. [ Method ] The progenies between Prunus armeniaca （ apricot ） Katy and Prunus salicina （plum） Moriteni were analyzed by means of RAPD and SSR molecular markers. [ Result] We found the differences in the electrophoresis bands between progenies and their parents, indicating that hybrid seedlings obtained in this study were real. [ Conclusion ] The combination of the above methods is effective and fast to identify the hybrids, and can shorten the breeding period.

杏NBS基因家族鉴定及其对果实抗病性的影响

【目的】探讨杏果实发育过程中NBS基因家族的表达规律,为果实抗病机制研究提供理论指导。【方法】运用生物信息学方法进行NBS基因家族鉴定与转录组分析。【结果】在串枝红杏基因组中共鉴定到155个NBS抗病基因,主要分为CNL型(126个)和NL型(29个),45%的基因主要分布在1号与2号染色体上,顺式作用元件较多且大多属于激素调控元件,发现1个杏特有的基序CNBS-1。NBS基因在不同品种发育过程中的基因表达模式类似,但在表达量上存在较大差异。8个NBS基因在串枝红果实发育的4个时期表达量均显著高于同时期大白杏,表明这些基因可能与串枝红果实抗病性高于大白杏有关。【结论】获得8个NBS差异表达基因,主要与抗致病疫霉病原与霜霉病原有关,为杏果实抗病育种提供理论指导。

不同浓度水杨酸处理对杏李恐龙蛋采后贮藏品质的影响

以杏李恐龙蛋(Prunus domestica×armeniaca konglongdan)为试验材料,研究了不同浓度水杨酸处理对其采后生理和贮藏品质的影响。结果表明,与对照相比,不同浓度水杨酸处理均能抑制恐龙蛋果实的乙烯释放速率,降低果实的呼吸强度,保持果实较高的硬度,降低果实失重率和腐烂率,此外,水杨酸处理可维持果实较高的可溶性固形物含量,同时显著提高果实的抗氧化酶活性。水杨酸处理能增强采后恐龙蛋果实的耐贮性和维持较高的贮藏品质,尤其以0.1 mmol/L水杨酸处理的保鲜效果最佳,可作为提高恐龙蛋果实采后耐贮性、延长其保鲜期的适宜处理条件。