Cyt b gene of North Sulawesi Tarsius sp., T. tumpara, T. sangirensis and T. tarsier (T. spectrum) had been partially sequenced. The homologous sequence of three groups had been compared to describe the phylogenetic po...Cyt b gene of North Sulawesi Tarsius sp., T. tumpara, T. sangirensis and T. tarsier (T. spectrum) had been partially sequenced. The homologous sequence of three groups had been compared to describe the phylogenetic position among them, as well as several other species accessed from the Genbank. Total DNA extracted from the muscular tissue had been obtained through tail cut sampling using the innuPREP DNA micro kit, and amplified using a pair of universal primer, L14841 and H15149. The size of the cyt b gene sequence amplified was 307 bp long. Sequence aligned using CLUSTAL-X program and diversity analysis were done using version 5.2.2. MEGA5 program. Genetic distance had been calculated by Tamura 3 parameter method and phylogenetic tree had been built using Neighbor-Joining and Maximum Likelihood methods. Genetic distance based on cyt b gene nucleotide was found from 0 to 0.240 with an average of 0.080. The phylogenetic tree constructed by Neighbor Joining and Maximum Likelihood methods indicated that T. tarsier, T. sangirensis and T. tumpara were closely related with Tarsius tarsier-complex, and distantly related with Cephalopachus bancanus and Carlito syrichta. The genetic distance and the phylogenetic tree had been constructed on the base of partial cyt b gene sequence of T. tarsier, T. sangirensis, T. tumpara and 5 other tarsier species and their accession. Those results are consistent with taxonomy based on morphology and vocal acoustic form.展开更多
构建了一个无中间体、无膜的微生物燃料电池,电池以生活污水为初始接种体,以醋酸钠水溶液为原料,考察了溶液浓度和电子受体等对电池性能的影响,分析了电池中微生物的反应动力学特征,并对电池中微生物的种属进行了鉴定。结果显示,微生物...构建了一个无中间体、无膜的微生物燃料电池,电池以生活污水为初始接种体,以醋酸钠水溶液为原料,考察了溶液浓度和电子受体等对电池性能的影响,分析了电池中微生物的反应动力学特征,并对电池中微生物的种属进行了鉴定。结果显示,微生物燃料电池中电流的产生主要是依靠电极表面吸附微生物直接将代谢产生的电子传递给电极表面所致;溶液COD的降解符合一级反应动力学特征;当溶液中存在O2和NO-3时溶液输出电压或电量均会降低,前者主要是发生好氧呼吸,后者主要是发生了反硝化作用。通过16 s rDNA的基因分析,确定了电池中的优势微生物为假单胞菌属。展开更多
分离到一株耐镉菌株XZC1,结合菌落特征和16S r DNA序列对其进行鉴定,将该菌在含Cd2+液体LB培养基中进行连续培养,获得菌体生长情况,分析该菌株镉耐性是否与质粒相关,检测菌株对其他几种重金属的耐性。结果表明,该菌株是Pseudomonas sp.,...分离到一株耐镉菌株XZC1,结合菌落特征和16S r DNA序列对其进行鉴定,将该菌在含Cd2+液体LB培养基中进行连续培养,获得菌体生长情况,分析该菌株镉耐性是否与质粒相关,检测菌株对其他几种重金属的耐性。结果表明,该菌株是Pseudomonas sp.,在Cd2+浓度为400mg/L LB培养基中可正常生长;经过质粒消除的XZC1不具有镉耐性,说明该菌株的镉耐性基因存在于大质粒上;XZC1对Fe2+、Cu2+、Mn2+、Ni2+的耐性较强,在400mg/L以上。菌株XZC1在重金属污染防治方面有深入研究价值。展开更多
SPS(Sanitary and Phyto-sanitary Agreement)(卫生与动植物检疫)措施是与风险密切相关的技术性贸易措施,SPS协议是用来规范成员国SPS措施的世贸组织协议,是成员国必须遵守的章程。目前SPS措施壁垒已成为中国禽肉出口的主要障碍。本文...SPS(Sanitary and Phyto-sanitary Agreement)(卫生与动植物检疫)措施是与风险密切相关的技术性贸易措施,SPS协议是用来规范成员国SPS措施的世贸组织协议,是成员国必须遵守的章程。目前SPS措施壁垒已成为中国禽肉出口的主要障碍。本文运用经济学局部均衡分析框架分析了SPS措施对我国禽肉出口的影响效应。研究认为,SPS措施对禽肉出口的短期影响为数量限制,而长期影响则表现为贸易限制(禁止)和贸易转移效应,最后用实证案例对SPS措施产生的贸易效应进行了验证。针对SPS措施的影响,认为加强我国防疫体系建设,建设无规定疫病区,严格控制药物使用和残留监控,采用HACCP认证等提高我国SPS水平,以较高的遵从成本换取更高的遵从收益。展开更多
文摘Cyt b gene of North Sulawesi Tarsius sp., T. tumpara, T. sangirensis and T. tarsier (T. spectrum) had been partially sequenced. The homologous sequence of three groups had been compared to describe the phylogenetic position among them, as well as several other species accessed from the Genbank. Total DNA extracted from the muscular tissue had been obtained through tail cut sampling using the innuPREP DNA micro kit, and amplified using a pair of universal primer, L14841 and H15149. The size of the cyt b gene sequence amplified was 307 bp long. Sequence aligned using CLUSTAL-X program and diversity analysis were done using version 5.2.2. MEGA5 program. Genetic distance had been calculated by Tamura 3 parameter method and phylogenetic tree had been built using Neighbor-Joining and Maximum Likelihood methods. Genetic distance based on cyt b gene nucleotide was found from 0 to 0.240 with an average of 0.080. The phylogenetic tree constructed by Neighbor Joining and Maximum Likelihood methods indicated that T. tarsier, T. sangirensis and T. tumpara were closely related with Tarsius tarsier-complex, and distantly related with Cephalopachus bancanus and Carlito syrichta. The genetic distance and the phylogenetic tree had been constructed on the base of partial cyt b gene sequence of T. tarsier, T. sangirensis, T. tumpara and 5 other tarsier species and their accession. Those results are consistent with taxonomy based on morphology and vocal acoustic form.
文摘构建了一个无中间体、无膜的微生物燃料电池,电池以生活污水为初始接种体,以醋酸钠水溶液为原料,考察了溶液浓度和电子受体等对电池性能的影响,分析了电池中微生物的反应动力学特征,并对电池中微生物的种属进行了鉴定。结果显示,微生物燃料电池中电流的产生主要是依靠电极表面吸附微生物直接将代谢产生的电子传递给电极表面所致;溶液COD的降解符合一级反应动力学特征;当溶液中存在O2和NO-3时溶液输出电压或电量均会降低,前者主要是发生好氧呼吸,后者主要是发生了反硝化作用。通过16 s rDNA的基因分析,确定了电池中的优势微生物为假单胞菌属。
文摘分离到一株耐镉菌株XZC1,结合菌落特征和16S r DNA序列对其进行鉴定,将该菌在含Cd2+液体LB培养基中进行连续培养,获得菌体生长情况,分析该菌株镉耐性是否与质粒相关,检测菌株对其他几种重金属的耐性。结果表明,该菌株是Pseudomonas sp.,在Cd2+浓度为400mg/L LB培养基中可正常生长;经过质粒消除的XZC1不具有镉耐性,说明该菌株的镉耐性基因存在于大质粒上;XZC1对Fe2+、Cu2+、Mn2+、Ni2+的耐性较强,在400mg/L以上。菌株XZC1在重金属污染防治方面有深入研究价值。
文摘SPS(Sanitary and Phyto-sanitary Agreement)(卫生与动植物检疫)措施是与风险密切相关的技术性贸易措施,SPS协议是用来规范成员国SPS措施的世贸组织协议,是成员国必须遵守的章程。目前SPS措施壁垒已成为中国禽肉出口的主要障碍。本文运用经济学局部均衡分析框架分析了SPS措施对我国禽肉出口的影响效应。研究认为,SPS措施对禽肉出口的短期影响为数量限制,而长期影响则表现为贸易限制(禁止)和贸易转移效应,最后用实证案例对SPS措施产生的贸易效应进行了验证。针对SPS措施的影响,认为加强我国防疫体系建设,建设无规定疫病区,严格控制药物使用和残留监控,采用HACCP认证等提高我国SPS水平,以较高的遵从成本换取更高的遵从收益。
