[Objective] This study aimed to test the effects of high-intensity pulsed electric field on the amino acid content in Pu’er tea leaves. [Method] The amino acid contents of fermented and unfermented the leaves of larg...[Objective] This study aimed to test the effects of high-intensity pulsed electric field on the amino acid content in Pu’er tea leaves. [Method] The amino acid contents of fermented and unfermented the leaves of large-leaf Pu’er tea variety produced in Yunnan Mengku, were measured before and after the treatment with high-intensity pulsed electric field （HPEF）. [Result] The difference of amino acid contents was notable between fermented and unfermented tea leaves produced in the same year. The amino acid content of unfermented tea leaves was larger than that in fermented tea leaves. The amino acid content in tea leaves decreased over time during storage. HPEF treatment under al the conditions improved the amino acid content in tea leaves. [Conclusion] This experiment provides theoretical foundation for the application of HPEF in processing of Pu’er tea leaves.展开更多
Powdery mildew, caused by Erysiphe pisi D.C., is an important disease of pea(Pisum sativum L.).The use of cultivars carrying powdery mildew resistance alleles at the er1 locus is the most effective and economical mean...Powdery mildew, caused by Erysiphe pisi D.C., is an important disease of pea(Pisum sativum L.).The use of cultivars carrying powdery mildew resistance alleles at the er1 locus is the most effective and economical means of controlling this disease. The objectives of this study were to screen Chinese elite pea cultivars for resistance to E. pisi and to identify the responsible gene at the er1 locus. Among the 37 pea cultivars tested, three(Yunwan 8, Yunwan 21, and Yunwan 23) were immune to E. pisi infection in phenotypic evaluations. The full-length cD NA sequences of the er1 candidate gene, PsM LO1, from the three resistant cultivars and control plants were analyzed. Comparison of the cD NA sequences of 10 clones revealed differences among the powdery mildew-resistant cultivars, susceptible controls, and wild-type cultivar Sprinter. The observed resistance in Yunwan 8 plants resulted from a point mutation(C → G) at position 680 of PsM LO1 that introduced a stop codon, leading to premature termination of protein synthesis. The responsible resistance allele was identified as er1–1. Powdery mildew resistance in Yunwan 21 and Yunwan 23 plants was caused by identical insertions or deletions in PsM LO1. Three distinct PsM LO1 transcripts were observed in Yunwan 21 and Yunwan 23 plants. These transcripts were characterized by a129-bp deletion and 155- and 220-bp insertions, respectively. The responsible resistance allele was identified as er1–2. We have characterized two important er1 alleles in three E. pisi-resistant pea cultivars bred in Yunnan Province, China. These cultivars represent important genetic resources for the breeding of powdery mildew-resistant pea cultivars.展开更多
The electron density recorded onboard the DEMETER satellite was analyzed to search for possible anomalies before earthquakes both in space and time. To distinguish pre-earthquake anomalies from the other anomalies rel...The electron density recorded onboard the DEMETER satellite was analyzed to search for possible anomalies before earthquakes both in space and time. To distinguish pre-earthquake anomalies from the other anomalies related to geomagnetic activity, data were filtered using the Kp index. The analysis is based on the comparison of data recorded closely to earthquakes in space and time and past data for the same area. In analyzing data around the time and location of the Pu＇er and Wenchuan earthquakes, obvious anomalies in electron density were found close to the epicenters, and some remarkable disturbances were detected before the earthquakes occurred. The results were finally compared with those of previous works that used the same data but employed different analysis methods. Good agreement was found which suggests that these anomalies have a close relation to the earthquake preparation.展开更多
The current situation of Pu'er tea in Yunnan was analyzed.The analysis result showed that the production output of Yunnan tea and the tea plantation area increased greatly when compared with the whole country,but ...The current situation of Pu'er tea in Yunnan was analyzed.The analysis result showed that the production output of Yunnan tea and the tea plantation area increased greatly when compared with the whole country,but the increase of Pu'er tea inclined to be stable.The restraining factors of Pu'er tea development were explored,and the corresponding countermeasures were put forward.It provided the basis for the sustainable development of Yunnan Pu'er tea.展开更多
数据质量评价是土地覆盖产品选择的依据和应用的前提。以云南省普洱市为研究区,采用面积相关系数、类型混淆分析、空间模式一致性分析以及混淆矩阵等方法,根据机载影像分类和亚米级影像目视判读结果,分析了该区的4种10m空间分辨率的土...数据质量评价是土地覆盖产品选择的依据和应用的前提。以云南省普洱市为研究区,采用面积相关系数、类型混淆分析、空间模式一致性分析以及混淆矩阵等方法,根据机载影像分类和亚米级影像目视判读结果,分析了该区的4种10m空间分辨率的土地覆盖产品(Pu′er LC,Esri_Land_Cover_2020_10m, ESA World Cover和Dynamic World)的精度和一致性。结果表明:1)4种土地覆盖产品的土地类型和分布格局基本一致,以林地为主,灌木与草地、耕地次之,面积相关性较高,相关系数均大于0.9;2)空间高一致性区域占普洱总面积的64.87%,以林地为主,空间一致性较低的区域内灌木与草地、林地、耕地、建设用地等土地覆盖类型交错分布;3)精度验证结果表明,Pu′er LC数据总体精度最高(88.52%),其他3个产品总体精度相差不大,在75%左右。研究可以为土地覆盖数据质量评价和使用等方面提供参考和借鉴。展开更多
The rare earth elements contents (La, Ce, Pr, Nd, Sm) in 150 Pu er tea samples were analyzed by using inductively coupled plasma atomic emission spectroscopy (ICP-AES), to evaluate the safety of Pu er tea. Among the i...The rare earth elements contents (La, Ce, Pr, Nd, Sm) in 150 Pu er tea samples were analyzed by using inductively coupled plasma atomic emission spectroscopy (ICP-AES), to evaluate the safety of Pu er tea. Among the investigated rare earth elements (REEs), Ce was the highest (35% of the total REEs content) whose concentrations were found to be in the range of 0.11–1.39 mg/kg, whereas that of Sm in the range 0.01–0.21 mg/kg, showing minimum levels (5.2%). La was the second highest element (28%) found in Pu e...展开更多
基金Supported by Scientific Research Fund from Department of Education of Yunnan Province(2012Z134C)~~
文摘[Objective] This study aimed to test the effects of high-intensity pulsed electric field on the amino acid content in Pu’er tea leaves. [Method] The amino acid contents of fermented and unfermented the leaves of large-leaf Pu’er tea variety produced in Yunnan Mengku, were measured before and after the treatment with high-intensity pulsed electric field （HPEF）. [Result] The difference of amino acid contents was notable between fermented and unfermented tea leaves produced in the same year. The amino acid content of unfermented tea leaves was larger than that in fermented tea leaves. The amino acid content in tea leaves decreased over time during storage. HPEF treatment under al the conditions improved the amino acid content in tea leaves. [Conclusion] This experiment provides theoretical foundation for the application of HPEF in processing of Pu’er tea leaves.
基金supported by the China Agriculture Research System (CARS-09)the Agricultural Science and Technology Program for Innovation Team on Identification and Excavation of Elite Crop Germplasm from Chinese Academy of Agricultural Sciences (CAAS)+1 种基金the Special Fund for Agro-scientific Research in the Public Interest (1610092015002-01) from the Institute of Crop Science, CAASthe Fund (2013BB010) from Science and Technology Department of Yunnan Province
文摘Powdery mildew, caused by Erysiphe pisi D.C., is an important disease of pea(Pisum sativum L.).The use of cultivars carrying powdery mildew resistance alleles at the er1 locus is the most effective and economical means of controlling this disease. The objectives of this study were to screen Chinese elite pea cultivars for resistance to E. pisi and to identify the responsible gene at the er1 locus. Among the 37 pea cultivars tested, three(Yunwan 8, Yunwan 21, and Yunwan 23) were immune to E. pisi infection in phenotypic evaluations. The full-length cD NA sequences of the er1 candidate gene, PsM LO1, from the three resistant cultivars and control plants were analyzed. Comparison of the cD NA sequences of 10 clones revealed differences among the powdery mildew-resistant cultivars, susceptible controls, and wild-type cultivar Sprinter. The observed resistance in Yunwan 8 plants resulted from a point mutation(C → G) at position 680 of PsM LO1 that introduced a stop codon, leading to premature termination of protein synthesis. The responsible resistance allele was identified as er1–1. Powdery mildew resistance in Yunwan 21 and Yunwan 23 plants was caused by identical insertions or deletions in PsM LO1. Three distinct PsM LO1 transcripts were observed in Yunwan 21 and Yunwan 23 plants. These transcripts were characterized by a129-bp deletion and 155- and 220-bp insertions, respectively. The responsible resistance allele was identified as er1–2. We have characterized two important er1 alleles in three E. pisi-resistant pea cultivars bred in Yunnan Province, China. These cultivars represent important genetic resources for the breeding of powdery mildew-resistant pea cultivars.
基金supported by the National Key Technology Research and Development Programme of China(No.2008BAC35B02)
文摘The electron density recorded onboard the DEMETER satellite was analyzed to search for possible anomalies before earthquakes both in space and time. To distinguish pre-earthquake anomalies from the other anomalies related to geomagnetic activity, data were filtered using the Kp index. The analysis is based on the comparison of data recorded closely to earthquakes in space and time and past data for the same area. In analyzing data around the time and location of the Pu＇er and Wenchuan earthquakes, obvious anomalies in electron density were found close to the epicenters, and some remarkable disturbances were detected before the earthquakes occurred. The results were finally compared with those of previous works that used the same data but employed different analysis methods. Good agreement was found which suggests that these anomalies have a close relation to the earthquake preparation.
基金Supported by the Earmarked Fund for Modern Agro-industry Technology Research System
文摘The current situation of Pu'er tea in Yunnan was analyzed.The analysis result showed that the production output of Yunnan tea and the tea plantation area increased greatly when compared with the whole country,but the increase of Pu'er tea inclined to be stable.The restraining factors of Pu'er tea development were explored,and the corresponding countermeasures were put forward.It provided the basis for the sustainable development of Yunnan Pu'er tea.
文摘数据质量评价是土地覆盖产品选择的依据和应用的前提。以云南省普洱市为研究区,采用面积相关系数、类型混淆分析、空间模式一致性分析以及混淆矩阵等方法,根据机载影像分类和亚米级影像目视判读结果,分析了该区的4种10m空间分辨率的土地覆盖产品(Pu′er LC,Esri_Land_Cover_2020_10m, ESA World Cover和Dynamic World)的精度和一致性。结果表明:1)4种土地覆盖产品的土地类型和分布格局基本一致,以林地为主,灌木与草地、耕地次之,面积相关性较高,相关系数均大于0.9;2)空间高一致性区域占普洱总面积的64.87%,以林地为主,空间一致性较低的区域内灌木与草地、林地、耕地、建设用地等土地覆盖类型交错分布;3)精度验证结果表明,Pu′er LC数据总体精度最高(88.52%),其他3个产品总体精度相差不大,在75%左右。研究可以为土地覆盖数据质量评价和使用等方面提供参考和借鉴。
基金Project supported by the Foundation of National Key Technology R & D Program (2007BAD58B05-3)
文摘The rare earth elements contents (La, Ce, Pr, Nd, Sm) in 150 Pu er tea samples were analyzed by using inductively coupled plasma atomic emission spectroscopy (ICP-AES), to evaluate the safety of Pu er tea. Among the investigated rare earth elements (REEs), Ce was the highest (35% of the total REEs content) whose concentrations were found to be in the range of 0.11–1.39 mg/kg, whereas that of Sm in the range 0.01–0.21 mg/kg, showing minimum levels (5.2%). La was the second highest element (28%) found in Pu e...
